With his publication in 1873 [1] J. W. Gibbs formulated the thermodynamic theory. It describes almost all macroscopically observed properties of matter and could also describe all phenomena if only the free energy U -...With his publication in 1873 [1] J. W. Gibbs formulated the thermodynamic theory. It describes almost all macroscopically observed properties of matter and could also describe all phenomena if only the free energy U - ST were explicitly known numerically. The thermodynamic uniqueness of the free energy obviously depends on that of the internal energy U and the entropy S, which in both cases Gibbs had been unable to specify. This uncertainty, lasting more than 100 years, was not eliminated either by Nernst’s hypothesis S = 0 at T = 0. This was not achieved till the advent of additional proof of the thermodynamic relation U = 0 at T = Tc. It is noteworthy that from purely thermodynamic consideration of intensive and extensive quantities it is possible to derive both Gibbs’s formulations of entropy and internal energy and their now established absolute reference values. Further proofs of the vanishing value of the internal energy at the critical point emanate from the fact that in the case of the saturated fluid both the internal energy and its phase-specific components can be represented as functions of the evaporation energy. Combining the differential expressions in Gibbs’s equation for the internal energy, d(μ/T)/d(1/T) and d(p/T)/d(1/T), to a new variable d(μ/T)/d(p/T) leads to a volume equation with the lower limit vc as boundary condition. By means of a variable transformation one obtains a functional equation for the sum of two dimensionless variables, each of them being related to an identical form of local interaction forces between fluid particles, but the different particle densities in the vapor and liquid spaces produce different interaction effects. The same functional equation also appears in another context relating to the internal energy. The solution of this equation can be given in analytic form and has been published [2] [3]. Using the solutions emerging in different sets of problems, one can calculate absolutely the internal energy as a function of temperature-dependent, phase-specific volumes and vapor pressure.展开更多
The international tidal gravity reference values at Wuhan station are determined accurately based on the comprehensive analysis of the tidal gravity observations obtained from 8 instruments. By comparing these with th...The international tidal gravity reference values at Wuhan station are determined accurately based on the comprehensive analysis of the tidal gravity observations obtained from 8 instruments. By comparing these with those in the tidal models given by Dehant (1997) while considering simultaneously (i) the global satellite altimeters tidal data, and (ii) the Schwiderski global tidal data and the local ones along the coast of China, it is found that the average discrepancy of the amplitude factors and of the phase differences for four main waves are given as 5.2% and 3.6% and as 0.16?and 0.08?respectively. They are improved evidently compared to those determined in early stage, indicating the important procedures in improving the Wuhan international tidal gravity reference values when including the long-series observations obtained with a superconducting gravimeter, and when considering the influence of the ocean loading and of the nearly daily free wobble of the Earth’s core.展开更多
AIM: To evaluate the level of sperm chromosome aberrations in male patients with hepatitis B, and to directly detect whether there are HBV DNA integrations in sperm chromosomes of hepatitis B patients.METHODS: Sperm c...AIM: To evaluate the level of sperm chromosome aberrations in male patients with hepatitis B, and to directly detect whether there are HBV DNA integrations in sperm chromosomes of hepatitis B patients.METHODS: Sperm chromosomes of 14 tested subjects (5healthy controls, 9 patients with HBV infection, including 1with acute hepatitis B, 2 with chronic active hepatitis B, 4with chronic persistent hepatitis B, 2 chronic HBsAg carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free golden hamster ova and human spermatozoa, and the frequencies of aberration spermatozoa were compared between subjects of HBV infection and controls. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm chromosomes.RESULTS: The total frequency of sperm chromosome aberrations in HBV infection group (14.8%, 33/223) was significantly higher than that in the control group (4.3%,5/116). Moreover, the sperm chromosomes in HBV infection patients commonly presented stickiness, clumping, failure to staining, etc, which would affect the analysis of sperm chromosomes. Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis. In 9 (9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots, others presented 2 to 4signals. There was significant difference of fluorescence intensity among the signal spots. The distribution of signal sites among chromosomes was random.CONCLUSION: HBV infection can bring about mutagenic effects on sperm chromosomes. Integrations of viral DNA into sperm chromosomes which are multisites and nonspecific, can further increase the instability of sperm chromosomes. This study suggested that HBV infection can create extensively hereditary effects by alteration genetic constituent and/or induction chromosome aberrations, as well as the possibility of vertical transmission of HBV via the germ line to the next generation.展开更多
AIM: To estimate the frequency of microdeletions in the long arm of Y-chromosome of 20 infertile males from South India. METHODS: Polymerase chain reaction (PCR) amplification using Y-specific STS of azoospermia facto...AIM: To estimate the frequency of microdeletions in the long arm of Y-chromosome of 20 infertile males from South India. METHODS: Polymerase chain reaction (PCR) amplification using Y-specific STS of azoospermia factor (AZF) regions i.e., SY 84 for AZFa, SY 127 for AZFb and SY 254 for AZFc. RESULTS: Of the 20 infertile subjects 3 (15 %), one azoospermic and two oligozoospermic, showed microdeletions in the AZF region of Y-chromosome. CONCLUSION: The frequency of deletions involving AZF region of the Y-chromosome is 15 % in azoospermic and severely oligozoospermic infertile men. PCR amplification of AZF locus is useful for the diagnosis of microdeletions in the Y-chromosome.展开更多
AIM: To investigate the effect of adrenalectomy (ADX) on the epididymidis of Sprague-Dawley rats. METHODS: The histological, biochemical (cholesterol protein, zinc, copper, alkaline and acid phosphatase aryl sulphatas...AIM: To investigate the effect of adrenalectomy (ADX) on the epididymidis of Sprague-Dawley rats. METHODS: The histological, biochemical (cholesterol protein, zinc, copper, alkaline and acid phosphatase aryl sulphatase, lactic dehydrogenase and leucine amino peptidase) and hormonal (FSH, LH and testosterone) changes of caput and cauda epididymis in ADX rats were observed. RESULTS: Organ wet weight, histological studies and morphometric measurements indicated a cellular degeneration in caput and cauda epididymis of ADX rats. Serum testosterone level was significantly lower in ADX than in sham-operated rats, while the serum FSH and LH were below the detection limit of 1 mIU/mL. The enzymatic activity was higher in ADX than in sham-operated rats. Epididymal zinc level increased whereas copper level decreased in ADX rats compared to the sham-operated. CONCLUSION: Adrenalectomy leads to degeneration of caput and cauda epididymidis epithelial cells as a result of decreased supply of testosterone.展开更多
文摘With his publication in 1873 [1] J. W. Gibbs formulated the thermodynamic theory. It describes almost all macroscopically observed properties of matter and could also describe all phenomena if only the free energy U - ST were explicitly known numerically. The thermodynamic uniqueness of the free energy obviously depends on that of the internal energy U and the entropy S, which in both cases Gibbs had been unable to specify. This uncertainty, lasting more than 100 years, was not eliminated either by Nernst’s hypothesis S = 0 at T = 0. This was not achieved till the advent of additional proof of the thermodynamic relation U = 0 at T = Tc. It is noteworthy that from purely thermodynamic consideration of intensive and extensive quantities it is possible to derive both Gibbs’s formulations of entropy and internal energy and their now established absolute reference values. Further proofs of the vanishing value of the internal energy at the critical point emanate from the fact that in the case of the saturated fluid both the internal energy and its phase-specific components can be represented as functions of the evaporation energy. Combining the differential expressions in Gibbs’s equation for the internal energy, d(μ/T)/d(1/T) and d(p/T)/d(1/T), to a new variable d(μ/T)/d(p/T) leads to a volume equation with the lower limit vc as boundary condition. By means of a variable transformation one obtains a functional equation for the sum of two dimensionless variables, each of them being related to an identical form of local interaction forces between fluid particles, but the different particle densities in the vapor and liquid spaces produce different interaction effects. The same functional equation also appears in another context relating to the internal energy. The solution of this equation can be given in analytic form and has been published [2] [3]. Using the solutions emerging in different sets of problems, one can calculate absolutely the internal energy as a function of temperature-dependent, phase-specific volumes and vapor pressure.
文摘The international tidal gravity reference values at Wuhan station are determined accurately based on the comprehensive analysis of the tidal gravity observations obtained from 8 instruments. By comparing these with those in the tidal models given by Dehant (1997) while considering simultaneously (i) the global satellite altimeters tidal data, and (ii) the Schwiderski global tidal data and the local ones along the coast of China, it is found that the average discrepancy of the amplitude factors and of the phase differences for four main waves are given as 5.2% and 3.6% and as 0.16?and 0.08?respectively. They are improved evidently compared to those determined in early stage, indicating the important procedures in improving the Wuhan international tidal gravity reference values when including the long-series observations obtained with a superconducting gravimeter, and when considering the influence of the ocean loading and of the nearly daily free wobble of the Earth’s core.
基金the Natural Science Foundation of Guangdong Province,No.940567the National Natural Science Foundation of China,No.39970374
文摘AIM: To evaluate the level of sperm chromosome aberrations in male patients with hepatitis B, and to directly detect whether there are HBV DNA integrations in sperm chromosomes of hepatitis B patients.METHODS: Sperm chromosomes of 14 tested subjects (5healthy controls, 9 patients with HBV infection, including 1with acute hepatitis B, 2 with chronic active hepatitis B, 4with chronic persistent hepatitis B, 2 chronic HBsAg carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free golden hamster ova and human spermatozoa, and the frequencies of aberration spermatozoa were compared between subjects of HBV infection and controls. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm chromosomes.RESULTS: The total frequency of sperm chromosome aberrations in HBV infection group (14.8%, 33/223) was significantly higher than that in the control group (4.3%,5/116). Moreover, the sperm chromosomes in HBV infection patients commonly presented stickiness, clumping, failure to staining, etc, which would affect the analysis of sperm chromosomes. Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis. In 9 (9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots, others presented 2 to 4signals. There was significant difference of fluorescence intensity among the signal spots. The distribution of signal sites among chromosomes was random.CONCLUSION: HBV infection can bring about mutagenic effects on sperm chromosomes. Integrations of viral DNA into sperm chromosomes which are multisites and nonspecific, can further increase the instability of sperm chromosomes. This study suggested that HBV infection can create extensively hereditary effects by alteration genetic constituent and/or induction chromosome aberrations, as well as the possibility of vertical transmission of HBV via the germ line to the next generation.
文摘AIM: To estimate the frequency of microdeletions in the long arm of Y-chromosome of 20 infertile males from South India. METHODS: Polymerase chain reaction (PCR) amplification using Y-specific STS of azoospermia factor (AZF) regions i.e., SY 84 for AZFa, SY 127 for AZFb and SY 254 for AZFc. RESULTS: Of the 20 infertile subjects 3 (15 %), one azoospermic and two oligozoospermic, showed microdeletions in the AZF region of Y-chromosome. CONCLUSION: The frequency of deletions involving AZF region of the Y-chromosome is 15 % in azoospermic and severely oligozoospermic infertile men. PCR amplification of AZF locus is useful for the diagnosis of microdeletions in the Y-chromosome.
文摘AIM: To investigate the effect of adrenalectomy (ADX) on the epididymidis of Sprague-Dawley rats. METHODS: The histological, biochemical (cholesterol protein, zinc, copper, alkaline and acid phosphatase aryl sulphatase, lactic dehydrogenase and leucine amino peptidase) and hormonal (FSH, LH and testosterone) changes of caput and cauda epididymis in ADX rats were observed. RESULTS: Organ wet weight, histological studies and morphometric measurements indicated a cellular degeneration in caput and cauda epididymis of ADX rats. Serum testosterone level was significantly lower in ADX than in sham-operated rats, while the serum FSH and LH were below the detection limit of 1 mIU/mL. The enzymatic activity was higher in ADX than in sham-operated rats. Epididymal zinc level increased whereas copper level decreased in ADX rats compared to the sham-operated. CONCLUSION: Adrenalectomy leads to degeneration of caput and cauda epididymidis epithelial cells as a result of decreased supply of testosterone.
