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GFPuv标记猕猴桃溃疡病菌的生物学特性及其在土壤、根系中的定殖 被引量:28
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作者 黄其玲 高小宁 +2 位作者 赵志博 秦虎强 黄丽丽 《中国农业科学》 CAS CSCD 北大核心 2013年第2期282-291,共10页
【目的】获得具有荧光标记的猕猴桃溃疡病菌(Pseudomonas syringae pv.actinidiae)菌株,为进一步揭示其侵染致病过程奠定基础。【方法】电击法对病菌进行GFPuv基因标记,应用荧光显微镜和平板稀释法研究标记菌株在土壤和根部的定殖情况... 【目的】获得具有荧光标记的猕猴桃溃疡病菌(Pseudomonas syringae pv.actinidiae)菌株,为进一步揭示其侵染致病过程奠定基础。【方法】电击法对病菌进行GFPuv基因标记,应用荧光显微镜和平板稀释法研究标记菌株在土壤和根部的定殖情况。【结果】GFPuv标记的猕猴桃溃疡病菌菌株在荧光显微镜下发出强烈的绿色荧光,8株标记菌基因组DNA中均扩增出约700 bp的目的片段。标记菌株PSAmx7-GFPuv1的菌体形态、生长曲线、最适温度、最适pH、致病性均与野生型无显著差异,其绿色荧光可稳定遗传。标记菌在灭菌土壤中可存活3个月左右,在未灭菌土壤中也能存活3周;灌根1 d检测,根表、根内组织中可分离到目标菌落,随后标记菌株数量呈现"先增后降"的趋势。【结论】电击法成功地将GFPuv基因转入猕猴桃溃疡病菌;导入的GFPuv基因对宿主菌的生物学特性没有影响;标记菌可在灭菌土壤中长期存活,并在根部定殖和增殖。 展开更多
关键词 猕猴桃溃疡病菌 pdsk-GFPuv 电击转化 土壤存活 根系定殖
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Biomechanical characteristics of an anterior cruciate ligament injury in javelin throwing 被引量:3
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作者 Boyi Dai Min Mao +1 位作者 William E. Garrett Bing Yu 《Journal of Sport and Health Science》 SCIE 2015年第4期333-340,共8页
Purpose: The purpose of this study was to understand the mechanism of an anterior cruciate ligament (ACL) injury in javelin throwing and javelin throwing techniques relevant to this ACL injury. Methods: The patien... Purpose: The purpose of this study was to understand the mechanism of an anterior cruciate ligament (ACL) injury in javelin throwing and javelin throwing techniques relevant to this ACL injury. Methods: The patient in this study was an elite female javelin thrower who completed the first three trials and sustained a non-contact ACL injury on her left knee in the fourth trial of javelin throwing during a recent track and field meet. Three-dimensional kinematic data were collected in the injury and non-injury trials. The kinematic data of 52 male and 54 female elite javelin throwers were obtained from a javelin throwing biomechanical database. Results: The patient had greater forward center of mass velocity and less vertical center of mass velocity after the first 25% of the delivery phase in the injury trial compared to non-injury trials. The patient had less left knee flexion angle and angular velocity but similar left knee valgus and internal rotation angles during the first 21% of the delivery phase in the injury trial compared to non-injury trials. The video images showed an obvious tibia anterior translation at the 30% of the delivery phase in the injury trial. The left knee flexion angle and angular velocity at the time of the left foot landing and the maximal left knee flexion angle during the delivery phase were not significantly correlated to the official distance for 52 male and 54 female elite javelin throwers. Conclusion: The ACL injury in this study occurred during the first 30% of the delivery phase, most likely during the first 25% of the delivery phase. A stiff landing of the left leg with a small knee flexion angle was the primary contributor to this injury. Javelin throwers may have a soft left leg landing with a flexed knee, which may help them prevent ACL injuries without compromising performance. 展开更多
关键词 ACL injury BIOMECHANICS Injury mechanism Injury prevention pdsk factors
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112例肝癌发病影响因素的病例对照研究 被引量:2
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作者 江飞 陈增瑞 《浙江预防医学》 2008年第2期3-5,共3页
目的了解浙江省玉环县海岛居民肝癌的主要危险因素为肝癌的病因预防和人群防制提供依据。方法以2004-2005年发现的原发性肝癌患者112例(男99例,女13例,年龄57.5±13.2岁)进行1:2配比对病例对照研究,并用条件Logistic回归模型对... 目的了解浙江省玉环县海岛居民肝癌的主要危险因素为肝癌的病因预防和人群防制提供依据。方法以2004-2005年发现的原发性肝癌患者112例(男99例,女13例,年龄57.5±13.2岁)进行1:2配比对病例对照研究,并用条件Logistic回归模型对所获资料进行单因素和多因素分析。结果多因素条件Logistic回归分析结果表明,父母恶性肿瘤史、乙型肝炎/HBsAg携带者、吃番茄、吃胡萝卜及吃腌制品的OR值分别为:6.03、246.48、4.02、0.05和5.70。结论乙型肝炎/HBsAg携带者、父母亲恶性肿瘤史者、吃腌制品(≥1次/周)、吃番茄可能是肝癌发生的危险因素;吃胡萝卜可能是肝癌发生的保护因素。 展开更多
关键词 原发性肝癌 危险因素 病例对照研究
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