mRNAs are transported within a plant through phloem.Aphids are phloem feeders and dodders(Cuscuta spp.)are parasites which establish phloem connections with host plants.When aphids feed on dodders,whether there is tra...mRNAs are transported within a plant through phloem.Aphids are phloem feeders and dodders(Cuscuta spp.)are parasites which establish phloem connections with host plants.When aphids feed on dodders,whether there is trafficking of mRNAs among aphids,dodders,and host plants and if aphid feeding a匚fects the mRNA transfer between dodders and hosts are unclear.We constructed a green peach aphid(GPA,Myzus persicae)-dodder(Cuscuta austra/is)-cucumber(Cucumis sativus)tritrophic system by infesting GPAs on C.australis,which parasitized cucumber hosts.We found that GPA feeding activated defense-related phytohormonal and transcriptomic responses in both C.australis and cucumbers and large numbers of mRNAs were found to be transferred between C.australis and cucumbers and between C.australis and GPAs;importantly,GPA feeding on C.australis greatly altered inter-species mobile mRNA profiles.Furthermore,three cucumber mRNAs and three GPA mRNAs could be respectively detected in GPAs and cucumbers.Moreover,our statistical analysis indicated that mRNAs with high abundances and long transcript lengths are likely to be mobile.This study reveals the existence of inter-species and even inter-kingdom mRNA movement among insects,parasitic plants,and parasite hosts,and suggests complex regulation of mRNA trafficking.展开更多
Peach aphid is a common pest and hard to detect.This study employs hyperspectral imaging technology to identify early damage in green cabbage caused by peach aphid.Through principal component transformation and multip...Peach aphid is a common pest and hard to detect.This study employs hyperspectral imaging technology to identify early damage in green cabbage caused by peach aphid.Through principal component transformation and multiple linear regression analysis,the correlation relation between spectral characteristics and infestation stage is analyzed.Then,four characteristic wavelength selection methods are compared and optimal characteristic wavelengths subset is determined to be input for modelling.One linear algorithm and two nonlinear modelling algorithms are compared.Finally,support vector machine(SVM)model based on the characteristic wavelengths selected by multi-cluster feature selection(MCFS)acquires the highest identification accuracy,which is 98.97%.These results indicate that hyperspectral imaging technology have the ability to identify early peach aphid infestation stages on green cabbages.展开更多
文摘mRNAs are transported within a plant through phloem.Aphids are phloem feeders and dodders(Cuscuta spp.)are parasites which establish phloem connections with host plants.When aphids feed on dodders,whether there is trafficking of mRNAs among aphids,dodders,and host plants and if aphid feeding a匚fects the mRNA transfer between dodders and hosts are unclear.We constructed a green peach aphid(GPA,Myzus persicae)-dodder(Cuscuta austra/is)-cucumber(Cucumis sativus)tritrophic system by infesting GPAs on C.australis,which parasitized cucumber hosts.We found that GPA feeding activated defense-related phytohormonal and transcriptomic responses in both C.australis and cucumbers and large numbers of mRNAs were found to be transferred between C.australis and cucumbers and between C.australis and GPAs;importantly,GPA feeding on C.australis greatly altered inter-species mobile mRNA profiles.Furthermore,three cucumber mRNAs and three GPA mRNAs could be respectively detected in GPAs and cucumbers.Moreover,our statistical analysis indicated that mRNAs with high abundances and long transcript lengths are likely to be mobile.This study reveals the existence of inter-species and even inter-kingdom mRNA movement among insects,parasitic plants,and parasite hosts,and suggests complex regulation of mRNA trafficking.
基金supported by China National Key Research and Development Program(No.2016YFD0700304)Shandong Natural Science Foundation Youth Program(No.ZR2021QC216)Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Science(No.CXGC2023A34)。
文摘Peach aphid is a common pest and hard to detect.This study employs hyperspectral imaging technology to identify early damage in green cabbage caused by peach aphid.Through principal component transformation and multiple linear regression analysis,the correlation relation between spectral characteristics and infestation stage is analyzed.Then,four characteristic wavelength selection methods are compared and optimal characteristic wavelengths subset is determined to be input for modelling.One linear algorithm and two nonlinear modelling algorithms are compared.Finally,support vector machine(SVM)model based on the characteristic wavelengths selected by multi-cluster feature selection(MCFS)acquires the highest identification accuracy,which is 98.97%.These results indicate that hyperspectral imaging technology have the ability to identify early peach aphid infestation stages on green cabbages.
文摘【目的】桃绿蚜(即桃蚜)是影响桃树早春生长的主要害虫,发掘桃抗蚜资源,阐明其遗传规律,促进抗性品种培育,是应对蚜虫危害的重要途径。【方法】利用‘粉寿星’(Prunus persica var.densa)为亲本材料,构建了6代遗传群体,对‘粉寿星’抗蚜性的遗传及其稳定性进行了分析。【结果】在抗蚜性分离群体中,抗性呈不连续分布,且具有较明显的双峰特征,抗性单株(RR或Rr)与敏感单株(rr)杂交,出现1∶0或1∶1的抗/感分离比例,抗性单株与抗性单株杂交(RR×R_或Rr×Rr)出现1∶0或3∶1的抗/感分离比例,感病单株之间杂交不出现抗性单株,抗性遗传6代未发现明显变化;连锁遗传分析表明,‘粉寿星’的抗蚜性与矮生性状没有连锁关系,与果肉黄/白色位点较近,遗传距离约为27.5 c M。【结论】‘粉寿星’对桃绿蚜的抗性为单基因控制显性遗传,并与果肉黄/白色位于同一连锁群,与矮生性状无连锁关系。本研究结果为抗蚜桃育种及其他相关研究奠定了基础。
