Appreciating a Chinese painting is a bit different from appreciating a Western painting.Chinese paintings are not overly realistic.For example,light,shadow and perspective are not stressed.One needs knowledge of Chine...Appreciating a Chinese painting is a bit different from appreciating a Western painting.Chinese paintings are not overly realistic.For example,light,shadow and perspective are not stressed.One needs knowledge of Chinese culture to understand the lingering charm and subtle nuances.This painting depicts peach flowers on the shores of a river in spring.My inspiration originated from'The Tale of the Peach Blossom Spring'展开更多
In order to accurately predict the flowering period of peach blossoms and provide visitors with the best viewing period of peach blossoms, the regression model was used to establish the forecasting model of the bloomi...In order to accurately predict the flowering period of peach blossoms and provide visitors with the best viewing period of peach blossoms, the regression model was used to establish the forecasting model of the blooming of ornamental peaches according to the relevant laws of phenology. The forecasting model could accurately predict the flowering period and the best viewing period of peach blossoms.展开更多
Differential thermal analysis was utilized to determine midwinter hardiness of stem tissues and flower buds of mei flower (Prunus mume) and David’s peach (Prunus davidiana). Low temperature freezing exotherms were fo...Differential thermal analysis was utilized to determine midwinter hardiness of stem tissues and flower buds of mei flower (Prunus mume) and David’s peach (Prunus davidiana). Low temperature freezing exotherms were found to occur at -35℃ in Green Calyxs’ David’s peach and -18℃ in mei flower. A Low temperature exotherm was produced by the freezing of deep supercooling water which was detected in the wood tissues but not in the bark. Freezing processes of the wood and those of the bark appeared to be independent. Deep supercooling points of both species were found to be closely related to freezing injury and to their respective distributions.展开更多
This study was conducted to assess the effect of gibberellin and its possible mechanism of action on peach flower formation. At flower induction, 100 mg L^-1 of gibberellic acid 3 （GA3） was sprayed on the leaves of ...This study was conducted to assess the effect of gibberellin and its possible mechanism of action on peach flower formation. At flower induction, 100 mg L^-1 of gibberellic acid 3 （GA3） was sprayed on the leaves of peach [Prunus persica （L.） Batsch.] cv. Bayuecui. Using anatomy, immunohistochemistry, and semi-quantitation, the in situ distribution of GAs and the expression of the key genes involved in peach flower formation in the apical meristem were studied during flowering differentiation. The results showed that induction of flowering in the Bayuecui peach occurred prior to 10 July in Beijing, China. Flower induction and further differentiation of the peach flower organs were significantly inhibited by leaf-spraying of GA3 at a concentration of 100 mg L^-1 during the induction stage. The flowering rate was only 11.67% after treatment. The distribution of GA1 in the apical meristem varied during the process of flower bud differentiation. From 13 June to 25 July, the GA1 signal from control plants was detected mainly in the vascular bundles at the base of the flower buds. No GA1 signal was detected in the apical meristem. After treatment with GA3, the distribution was similar to that of the control from 13 June to 3 July. On 13 July, a GA1 signal was detected in the apical meristem accompanied by an increase in the GA1 signal in the vascular bundles at the base of the flower buds. The GA1 signal weakened significantly in both the vascular bundles and the apical meristem on 25 July. The expression of the genes PpLEAFY and MADS6 in flower buds could be detected only on 10 October in the GA3-treated plants. The critical period for flower induction of Bayuecui peach in Beijing was in early July, during which time, leaf-spraying with 100 mg L-1 GA3 could effectively inhibit flower induction and further differentiation of the flower buds. GA1 in the gibberellin family was the suppressor for flower induction in peach. Its action was affected by the stage of flower bud differentiation. Expression of the key genes PpLEAFY and MADS6 involved in flower formation was inhibited by GA3 treatment.展开更多
MADS box proteins play an important role in floral development. To find genes involved in the floral transition of Prunus species, cDNAs for two MADS box genes, PpMADS1 and PpMADSIO, were cloned using degenerate prime...MADS box proteins play an important role in floral development. To find genes involved in the floral transition of Prunus species, cDNAs for two MADS box genes, PpMADS1 and PpMADSIO, were cloned using degenerate primers and 5＇- and T-RACE based on the sequence database of P. persiea and P. duleis. The full length of PpMADS1 cDNA is 1,071 bp containing an open reading frame （ORF） of 717 bp and coding for a polypeptide of 238 amino acid residues. The full length of PpMADSIO cDNA is 937 bp containing an ORF of 633 bp and coding for a polypeptide of 210 amino acid residues. Sequence comparison revealed that PpMADS1 and PpMADSIO were highly homologous to genes API and PI in Arabidopsis, respectively. Phylogenetic analysis indicated that PpMADS1 belongs to the euAP1 clade of class A, and PpMADSIO is a member of GLO/PI clade of class B. RT-PCR analysis showed that PpMADS1 was expressed in sepal, petal, carpel, and fruit, which was slightly different from the expression pattern ofAPl; PpMADS10 was expressed in petal and stamen, which shared the same expression pattern as PI. Using selective mapping strategy, PpMADSI was assigned onto the Binl：50 on the G1 linkage group between the markers MCO44 and TSA2, and PpMADSIO onto the Bin1：73 on the same linkage group between the markers Lap- 1 and FGA8. Our results provided the basis for further dissection of the two MADS box gene function.展开更多
【目的】进一步完善桃花型基因型分类,开发桃花型相关分子标记,为观赏桃花的分子辅助育种提供理论支持。【方法】利用全基因组关联分析(genome-wide association study,GWAS)、IGV可视化软件分析,以及竞争性等位基因特异性PCR(Kompetiti...【目的】进一步完善桃花型基因型分类,开发桃花型相关分子标记,为观赏桃花的分子辅助育种提供理论支持。【方法】利用全基因组关联分析(genome-wide association study,GWAS)、IGV可视化软件分析,以及竞争性等位基因特异性PCR(Kompetitive allele-specific PCR,KASP)技术,在定位到的候选位点进行鉴定分析。【结果】GWAS定位分析在Pp08:14518604~14521291 bp存在一个ms179810转座子的缺失,x2验证结果表明,转座子的插入与缺失与花型性状无显著性关系。在转座子上游33980 bp处鉴定到一个单碱基核苷酸(SNP)变异,变异类型分为CC型、AC型、AA型3种,比对结果显示基因型为CC型、AC型,其表型为铃形,基因型为AA型,其表型为蔷薇形,在145份桃自然群体中鉴定准确率为98.62%。但是通过表型比对发现,基因型为CC时,铃形花冠直径为(1.54±0.46)cm,同时,基因型杂合的中蟠17号自交群体后代192株中基因型与表型准确率在98.44%。【结论】根据桃基因组中Pp08:14484624 bp处的变异类型以及桃花型的调查结果,首次将铃形花基因型细分为纯合铃形和杂合铃形,且开发了同时鉴定纯合铃形、杂合铃形及蔷薇形的分子标记。展开更多
文摘Appreciating a Chinese painting is a bit different from appreciating a Western painting.Chinese paintings are not overly realistic.For example,light,shadow and perspective are not stressed.One needs knowledge of Chinese culture to understand the lingering charm and subtle nuances.This painting depicts peach flowers on the shores of a river in spring.My inspiration originated from'The Tale of the Peach Blossom Spring'
文摘In order to accurately predict the flowering period of peach blossoms and provide visitors with the best viewing period of peach blossoms, the regression model was used to establish the forecasting model of the blooming of ornamental peaches according to the relevant laws of phenology. The forecasting model could accurately predict the flowering period and the best viewing period of peach blossoms.
基金This project was supported by the National Natural Science Foundation of China
文摘Differential thermal analysis was utilized to determine midwinter hardiness of stem tissues and flower buds of mei flower (Prunus mume) and David’s peach (Prunus davidiana). Low temperature freezing exotherms were found to occur at -35℃ in Green Calyxs’ David’s peach and -18℃ in mei flower. A Low temperature exotherm was produced by the freezing of deep supercooling water which was detected in the wood tissues but not in the bark. Freezing processes of the wood and those of the bark appeared to be independent. Deep supercooling points of both species were found to be closely related to freezing injury and to their respective distributions.
文摘This study was conducted to assess the effect of gibberellin and its possible mechanism of action on peach flower formation. At flower induction, 100 mg L^-1 of gibberellic acid 3 （GA3） was sprayed on the leaves of peach [Prunus persica （L.） Batsch.] cv. Bayuecui. Using anatomy, immunohistochemistry, and semi-quantitation, the in situ distribution of GAs and the expression of the key genes involved in peach flower formation in the apical meristem were studied during flowering differentiation. The results showed that induction of flowering in the Bayuecui peach occurred prior to 10 July in Beijing, China. Flower induction and further differentiation of the peach flower organs were significantly inhibited by leaf-spraying of GA3 at a concentration of 100 mg L^-1 during the induction stage. The flowering rate was only 11.67% after treatment. The distribution of GA1 in the apical meristem varied during the process of flower bud differentiation. From 13 June to 25 July, the GA1 signal from control plants was detected mainly in the vascular bundles at the base of the flower buds. No GA1 signal was detected in the apical meristem. After treatment with GA3, the distribution was similar to that of the control from 13 June to 3 July. On 13 July, a GA1 signal was detected in the apical meristem accompanied by an increase in the GA1 signal in the vascular bundles at the base of the flower buds. The GA1 signal weakened significantly in both the vascular bundles and the apical meristem on 25 July. The expression of the genes PpLEAFY and MADS6 in flower buds could be detected only on 10 October in the GA3-treated plants. The critical period for flower induction of Bayuecui peach in Beijing was in early July, during which time, leaf-spraying with 100 mg L-1 GA3 could effectively inhibit flower induction and further differentiation of the flower buds. GA1 in the gibberellin family was the suppressor for flower induction in peach. Its action was affected by the stage of flower bud differentiation. Expression of the key genes PpLEAFY and MADS6 involved in flower formation was inhibited by GA3 treatment.
基金supported by the National Natural Science Foundation of China(No.30500395)the National High Technology Research and Development Program(863 Projects)of China(No.2006AA10Z130 and 2006AA100108-3-7).
文摘MADS box proteins play an important role in floral development. To find genes involved in the floral transition of Prunus species, cDNAs for two MADS box genes, PpMADS1 and PpMADSIO, were cloned using degenerate primers and 5＇- and T-RACE based on the sequence database of P. persiea and P. duleis. The full length of PpMADS1 cDNA is 1,071 bp containing an open reading frame （ORF） of 717 bp and coding for a polypeptide of 238 amino acid residues. The full length of PpMADSIO cDNA is 937 bp containing an ORF of 633 bp and coding for a polypeptide of 210 amino acid residues. Sequence comparison revealed that PpMADS1 and PpMADSIO were highly homologous to genes API and PI in Arabidopsis, respectively. Phylogenetic analysis indicated that PpMADS1 belongs to the euAP1 clade of class A, and PpMADSIO is a member of GLO/PI clade of class B. RT-PCR analysis showed that PpMADS1 was expressed in sepal, petal, carpel, and fruit, which was slightly different from the expression pattern ofAPl; PpMADS10 was expressed in petal and stamen, which shared the same expression pattern as PI. Using selective mapping strategy, PpMADSI was assigned onto the Binl：50 on the G1 linkage group between the markers MCO44 and TSA2, and PpMADSIO onto the Bin1：73 on the same linkage group between the markers Lap- 1 and FGA8. Our results provided the basis for further dissection of the two MADS box gene function.
