Antioxidative and neuroprotective activities of peanut sprout extracts against oxidative stress in SK-N-SH cells

Objective: To evaluate the protective effect of peanut sprout extract(PSE) against paraquat(PQ) induced SK-N-SH cells.Methods: Three groups of cells were used in the experiment, together with a fourth,control group. One group was treated with PQ, the second group was treated with PSE,and the third group was pre-treated with PSE. The control group was untreated. Cell viability and toxicity were detected by MTT assay, cellular reactive oxygen species(ROS) was detected by Muse Cell Analyzer, quantitative RT-PCR was applied to investigate the expression of SIRT1 and a-synuclein genes, and A b42 was detected by western blot.Results: The 50% effective concentration of PQ was 0.75 mmol/L. PSE had no significant cytotoxicity at a concentration of 1.5 mg/m L. In the group of cells pre-treated with PSE, cell death was significantly inhibited. In the PQ treated group, PQ was increased in the intracellular ROS in the cells. Intracellular ROS was significantly decreased in the cells treated with PSE and also those pre-treated with PSE. PSE significantly downregulated the expression of SIRT1 and a-syn genes, and it was found that PQ significantly increased b-amyloid 42 levels whereas this action was inhibited by PSE.Conclusions: PSE has neuroprotective activities against oxidative stress in SK-N-SH cells induced by PQ, suggesting that PSE is a highly promising agent in the prevention of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.

花生芽中白藜芦醇的亚临界水萃取及抑制酶活性研究

为了提高花生芽中白藜芦醇的提取率,此试验通过考察液料比、亚临界萃取温度、萃取时间、提取次数对花生芽中白藜芦醇提取率的影响,在单因素试验基础上进行响应面优化亚临界水萃取花生芽中白藜芦醇的工艺。并通过测定白藜芦醇纯化物抑制α-淀粉酶和α-葡萄糖苷酶活性的能力研究其抑制酶活性。结果表明,亚临界水萃取花生芽中白藜芦醇的最优工艺为:萃取温度135℃,萃取时间30 min,液料比130∶1(m L/g),在此条件下整个试验过程重复三次,白藜芦醇提取率为(7.65±0.04)mg/g。花生芽白藜芦醇具有良好的抑制酶活性能力,其对α-淀粉酶和α-葡萄糖苷酶的抑制率分别为67.36%、73.38%。

花生芽白藜芦醇超声-微波协同提取及其抗氧化活性研究

以花生芽为原料,采用超声-微波协同法提取花生芽白藜芦醇。以料液比、乙醇浓度、微波时间、微波功率、超声时间、超声功率为单因素,以花生芽白藜芦醇提取含量为考察指标,通过响应面试验确定超声-微波协同法提取花生芽白藜芦醇的最佳工艺参数为:料液比1∶35、乙醇浓度60%、微波时间120 s、微波功率400 W、超声时间3 min、超声功率290 W。在此条件下花生芽白藜芦醇提取含量为8.64 mg/g。抗氧化试验研究表明,花生芽白藜芦醇对DPPH自由基的清除能力略低于维生素C,但对ABTS自由基的清除能力强于维生素C;另外,花生芽白藜芦醇对α-葡萄糖苷酶表现出较强的抑制能力,具有较好的体外降血糖作用。因此,超声-微波协同提取是一种高效的白藜芦醇提取方法,且作为一种新型白藜芦醇资源,花生芽白藜芦醇有着潜在应用价值。

超声波辅助提取花生芽中酚类物质及不同组分的多酚分布

以花生芽菜为原料,通过单因素和正交试验对超声波辅助提取酚类物质的工艺进行优化,并对不同溶剂提取组分、洗脱组分和萃取组分中的多酚分布及其抗氧化活性进行了比较。结果表明:超声波辅助提取花生芽中酚类物质的最佳提取工艺为乙醇体积分数70%、超声温度60℃、超声时间80 min、料液比1∶30(g/m L),在此条件下总酚提取量为0.801 mg/g。水提组分多酚含量最高,体积分数70%乙醇提取组分抗氧化活性最强并且对酚类物质的洗脱率最高,但体积分数30%乙醇可洗脱出抗氧化活性最强的组分。正丁醇是较为理想的花生芽酚类物质萃取溶剂,其对酚类物质的萃取率为61.2%,对DPPH自由基清除率可达98.3%。