Effect of pediatric tuina on hypothalamic metabolites in young rabbits using liquid chromatography-mass spectrometry

Objective: To investigate the changes in the hypothalamic metabolites of lipopolysaccharide(LPS) febrile young rabbits after the treatment with pediatric tuina.Methods: A total of 30 young rabbits were randomly assigned into three groups: the normal group, the model group, and the tuina group. Both the model group and the tuina group were injected intravenously with LPS. “Six antipyretic manipulations”(pushing Tianmen, pushing Kangong, kneading Taiyang,kneading Erhougaogu, clearing Tianheshui, and pushing Jizhu) were administered 1 h after the LPS injection in the tuina group. The rectal temperatures of the young rabbits were monitored during the experiment to explore the antipyretic effect. Three hours after the injection, the content of interleukin-1β(IL-1β), tumor necrosis factor(TNF)-a, and prostaglandin E;(PGE;) in the serum was detected. In addition, liquid chromatography-mass spectrometry(LC-MS) was used for the hypothalamus metabolomics.Results: Compared with the model group, the rectal temperature of the tuina group was decreased at 2 h and 3 h after the LPS injection(P =.04, P =.03, respectively), and the content of IL-1β, TNF-a, and PGE2was decreased(P =.03, P =.003, and P =.008, respectively). The metabolomics results showed that there were 23 potential biomarkers after the tuina intervention, enriching 27 pathways. Lipid metabolites,especially glycerophospholipids, were a majority of the altered metabolites. The primary metabolic pathways affected by tuina included the arachidonic acid metabolism, the GABAergic synapse, D-glutamine and D-glutamate metabolism, and the glutamatergic synapse.Conclusion: Pediatric tuina reduced the temperature of the febrile rabbits and downregulated the expression of IL-1β, TNF-a, and PGE2, and the antipyretic mechanism may be related to changes in metabolites and metabolic pathways in the hypothalamus.

“四明穴”推拿联合耳穴贴压治疗儿童屈光参差性弱视17例

The article introduced the application of Tuina at"Simingxue"(four points named after"ming")and pressing therapy at auricular points in treatment of 17 cases of anisometropic amblyopia in children.The methods:pressing and kneading with the thumb at Jingming(睛明BL1),Shangming(上明Extra),Yiming(翳明EX-HN14)and Guangming(光明GB37).Pressing with the finger digital at Yintang(印堂EX-HN3),Cuanzhu(攒竹BL2),Yuyao(鱼腰EX-HN4),Taiyang(太阳EX-HN5),Yingbai(阳白GB14),Sizhukong(丝竹空TE23),Tongziliao(瞳子髎GB1),Qiuhou(球后EX-HN7)and Chengqi(承泣ST1).Scraping the upper and lower orbits,nipping the eyebrows,pushing the orbits,pushing baoping and warming the eyes.Pressing Quchi(曲池LI11),Hegu(合谷LI4),GB37,Zusanli(足三里ST36),Xuehai(血海SP10)and Sanyinjiao(三阴交SP6).Rolling on the bilateral distributions of bladder meridian on the back.Pressing and kneading at Touwei(头维ST8),Qucha(曲差BL4),Baihui(百会GV20),Sishencong(四神聪EX-HN1),Fengchi(风池GB20)and Dazhui(大椎GV14).Grasping five meridians,scattering bilateral temporal regions,grasping and kneading the neck and nape region and holding Jianjing(肩井GB21).Auricular pressing therapy was exerted at Yan(眼eye,LO5),Gan(肝liver,CO12),Shen(肾kidney,CO10),Pi(脾spleen,CO13),Wei(胃stomach,CO4),Pizhixia(皮质下subcortex,AT4),Pingjianqian(屏间前anterior intertragic notch,TG21)and Pingjianhou屏间后(posterior intertragic notch,AT11).The treatment was given 3 times a week,6 treatments made one course and 4 courses of treatment were required.Of 17 patients,2 cases were cured,13 cases cured basically,1 case improved and 1 case failed.The total effective rate was 88.2%.

小儿拇指狭窄性腱鞘炎案

A female patient,13 months old,suffering from limited extension of the right thumb for 7 days,diagnosed as stenosing tenosynovitis of the thumb in children.Tuina(Chinese massage) therapy was adopted,in which,stimulation was applied with fingers instead of needles,including pressing and kneading,twisting and kneading,plucking,dorsal extending,rotating as well as pushing and rubbing manipulations.The whole procedure of manipulation lasted around 15 min,once every two days,3 times weekly.Meanwhile,the parents were advised to bath the child patient's right hand into warm water every day and fix the affected thumb with splint at night.After 12 treatments with tuina therapy,the flexion and extension function of the affected thumb were returned to normal and the thumb motor function was recovered.In 2 months of follow-up,the thumb motor function was normal and the stenosing tenosynovitis was not recurred.In this case,tuina therapy is adopted in treatment of stenosing tenosynovitis of the thumb in children.As an alternative method of surgery,tuina therapy has a satisfactory clinical effect.