The soft rot infected by pathogenic bacterium Erwinia aroideae Holland is one of the three serious diseases of Chinese cabbage ( Brassica pekinensis Rupr.). By constructing vector system of high frequency transformati...The soft rot infected by pathogenic bacterium Erwinia aroideae Holland is one of the three serious diseases of Chinese cabbage ( Brassica pekinensis Rupr.). By constructing vector system of high frequency transformation mediated by Agrobacterium tunefaciens EHA105, anti-bacterial peptide gene with strong bactericidal action to pathogenic bacteria was introduced into Chinese cabbage AB-81 self-bred line and the transgenic plants were obtained. PCR and Southern blotting detection showed that target gene was integrated into plant genome of Chinese cabbage. The tests of bacteriostasis action of the extract from transgenic plants in vitro, and the assay of disease-resistant of transgenic plantlets in the tube and the pot by perfusing inoculation with pathogenic bacteria showed obvious resistance to soft rot. This resistance can be a stable heredity by genetic analysis of generations of transgenic plants self-bred, separation ratio of its R, was 3:1. The resistance to Km and disease of soft rot was still kept in the R-5. These results indicated the possibility of breeding new varieties of anti-soft rot Chinese cabbage by transgenic plants as parents.展开更多
A molecular genetic map of Chinese cabbage was constructed with a 102 recombinant inbred (RI) population from a cross of two cultivated Chinese cabbage lines 177 and 276, using AFLP and RAPD markers. 352 markers inclu...A molecular genetic map of Chinese cabbage was constructed with a 102 recombinant inbred (RI) population from a cross of two cultivated Chinese cabbage lines 177 and 276, using AFLP and RAPD markers. 352 markers including 265 AFLP markers and 87 RAPD markers were integrated into 17 linkage groups. It covered a total of 2 665. 7 cM with an average interval of 7. 6 cM. AFLP marker is efficient for map construction while it easily forms clusters to cause big gaps in map. A total of 13.92 % abnormal segregation markers distributed in the map. The molecular genetic map is fundamental for gene localization, comparative genomics, and QTL mapping of important agronomic traits.展开更多
The Beijing Swift(Apus apus pekinensis)is a typical cavity-nesting bird that often nests inside holes and crevices in old architectures.Direct observation of their breeding behaviour is challenging and their breeding ...The Beijing Swift(Apus apus pekinensis)is a typical cavity-nesting bird that often nests inside holes and crevices in old architectures.Direct observation of their breeding behaviour is challenging and their breeding ecology is thus poorly studied.In this study,we analysed light-level geolocation data collected from six Beijing Swifts for the first time.Our results showed that geolocators can make comprehensive inference of their incubation period and behaviour.As a cost-effective and non-invasive method,geolocators can not only facilitate discovering migration routes,but also can be widely applied in the study of avian reproductive behaviour,especially in cavity-nesting bird species.We further discussed the characteristics and merits of this method and compared with other conventional nest-monitoring methods in recording birds.展开更多
[Objectives]To explore the difference of purgative effect of Mongolian medicine Euphorbia pekinensis and Pharbitidis Semen(black)by animal experiment,taking Euphorbia pekinensis and Pharbitidis Semen(black)as the rese...[Objectives]To explore the difference of purgative effect of Mongolian medicine Euphorbia pekinensis and Pharbitidis Semen(black)by animal experiment,taking Euphorbia pekinensis and Pharbitidis Semen(black)as the research object.[Methods]Sixty Kunming mice were randomly divided into blank group(n=20),Euphorbia pekinensis group(n=20)and Pharbitidis Semen(black)group(n=20).After fasted but provided with free acess to drinking water for 3 d,the mice were intragastrically administered with drinking water,Euphorbia pekinensis test solution and Pharbitidis Semen(black)test solution,respectively.The mice were then placed separately in a mouse cage covered with filter paper for purgation test and intestinal propulsion test.[Results]The time of the first purgation in the Euphorbia pekinensis group and the Pharbitidis Semen(black)group was longer than that in the blank group(P<0.05).The total times of purgation and defecation within 5 h in the Euphorbia pekinensis group and the Pharbitidis Semen(black)group were higher than those in the blank group(P<0.05);the purgation index and intestinal propulsion rate in the Euphorbia pekinensis group and the Pharbitidis Semen(black)group were higher than those in the blank group(P<0.05).[Conclusions]The Mongolian medicine Euphorbia pekinensis was similar to Pharbitidis Semen(black)in purgation test and intestinal propulsion test,and could play a substitute role.展开更多
文摘The soft rot infected by pathogenic bacterium Erwinia aroideae Holland is one of the three serious diseases of Chinese cabbage ( Brassica pekinensis Rupr.). By constructing vector system of high frequency transformation mediated by Agrobacterium tunefaciens EHA105, anti-bacterial peptide gene with strong bactericidal action to pathogenic bacteria was introduced into Chinese cabbage AB-81 self-bred line and the transgenic plants were obtained. PCR and Southern blotting detection showed that target gene was integrated into plant genome of Chinese cabbage. The tests of bacteriostasis action of the extract from transgenic plants in vitro, and the assay of disease-resistant of transgenic plantlets in the tube and the pot by perfusing inoculation with pathogenic bacteria showed obvious resistance to soft rot. This resistance can be a stable heredity by genetic analysis of generations of transgenic plants self-bred, separation ratio of its R, was 3:1. The resistance to Km and disease of soft rot was still kept in the R-5. These results indicated the possibility of breeding new varieties of anti-soft rot Chinese cabbage by transgenic plants as parents.
文摘A molecular genetic map of Chinese cabbage was constructed with a 102 recombinant inbred (RI) population from a cross of two cultivated Chinese cabbage lines 177 and 276, using AFLP and RAPD markers. 352 markers including 265 AFLP markers and 87 RAPD markers were integrated into 17 linkage groups. It covered a total of 2 665. 7 cM with an average interval of 7. 6 cM. AFLP marker is efficient for map construction while it easily forms clusters to cause big gaps in map. A total of 13.92 % abnormal segregation markers distributed in the map. The molecular genetic map is fundamental for gene localization, comparative genomics, and QTL mapping of important agronomic traits.
基金funded by SHAN SHUI Conservation Centre,Beijing。
文摘The Beijing Swift(Apus apus pekinensis)is a typical cavity-nesting bird that often nests inside holes and crevices in old architectures.Direct observation of their breeding behaviour is challenging and their breeding ecology is thus poorly studied.In this study,we analysed light-level geolocation data collected from six Beijing Swifts for the first time.Our results showed that geolocators can make comprehensive inference of their incubation period and behaviour.As a cost-effective and non-invasive method,geolocators can not only facilitate discovering migration routes,but also can be widely applied in the study of avian reproductive behaviour,especially in cavity-nesting bird species.We further discussed the characteristics and merits of this method and compared with other conventional nest-monitoring methods in recording birds.
文摘[Objectives]To explore the difference of purgative effect of Mongolian medicine Euphorbia pekinensis and Pharbitidis Semen(black)by animal experiment,taking Euphorbia pekinensis and Pharbitidis Semen(black)as the research object.[Methods]Sixty Kunming mice were randomly divided into blank group(n=20),Euphorbia pekinensis group(n=20)and Pharbitidis Semen(black)group(n=20).After fasted but provided with free acess to drinking water for 3 d,the mice were intragastrically administered with drinking water,Euphorbia pekinensis test solution and Pharbitidis Semen(black)test solution,respectively.The mice were then placed separately in a mouse cage covered with filter paper for purgation test and intestinal propulsion test.[Results]The time of the first purgation in the Euphorbia pekinensis group and the Pharbitidis Semen(black)group was longer than that in the blank group(P<0.05).The total times of purgation and defecation within 5 h in the Euphorbia pekinensis group and the Pharbitidis Semen(black)group were higher than those in the blank group(P<0.05);the purgation index and intestinal propulsion rate in the Euphorbia pekinensis group and the Pharbitidis Semen(black)group were higher than those in the blank group(P<0.05).[Conclusions]The Mongolian medicine Euphorbia pekinensis was similar to Pharbitidis Semen(black)in purgation test and intestinal propulsion test,and could play a substitute role.
