A substantial fraction of miRNA^＊ species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA^＊ species in duck skin by using Solexa sequencing. We obtained ...A substantial fraction of miRNA^＊ species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA^＊ species in duck skin by using Solexa sequencing. We obtained a total of 96 miRNA^＊ species in two skin small RNA libraries and identified 56 miRNA/miRNA^＊ （miR/miR^＊） pairs. Nucleotide bias of miRNA^＊ indicated that the priority was C〉A〉U〉G for the first nucleotide and U〉C〉A〉G for the last nucleotide. Comparison analyses showed that 3＇-U accounted for a higher proportion in the 56 miR/miR＊ pairs. Among the top 20 expressed miRNA^＊ species, 17 were shared by two libraries and most of the miRNA^＊ species were highly conservative, especially in the ＂seed region＂, miR-199a^＊ were expressed highly in our samples, which was also previously shown abundant in mouse hair follicle. Furthermore, four miRNA^＊ species were predicted to target their genes in signal pathways of feather follicle development and feather morphogenesis despite very low levels.展开更多
基金supported by the Open Fund of GuangdongProvincial Key Laboratory of Agro-Animal Genomics andMolecular Breeding, South China Agricultural University,China (201004)
文摘A substantial fraction of miRNA^＊ species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA^＊ species in duck skin by using Solexa sequencing. We obtained a total of 96 miRNA^＊ species in two skin small RNA libraries and identified 56 miRNA/miRNA^＊ （miR/miR^＊） pairs. Nucleotide bias of miRNA^＊ indicated that the priority was C〉A〉U〉G for the first nucleotide and U〉C〉A〉G for the last nucleotide. Comparison analyses showed that 3＇-U accounted for a higher proportion in the 56 miR/miR＊ pairs. Among the top 20 expressed miRNA^＊ species, 17 were shared by two libraries and most of the miRNA^＊ species were highly conservative, especially in the ＂seed region＂, miR-199a^＊ were expressed highly in our samples, which was also previously shown abundant in mouse hair follicle. Furthermore, four miRNA^＊ species were predicted to target their genes in signal pathways of feather follicle development and feather morphogenesis despite very low levels.
