As a Chinese medicinal plant,Smilax china is traditionally used in the treatment of pelvic inflammatory disease(PID).Flavonoids have been identified as the anti-inflammatory bioactive fraction.However,the high content...As a Chinese medicinal plant,Smilax china is traditionally used in the treatment of pelvic inflammatory disease(PID).Flavonoids have been identified as the anti-inflammatory bioactive fraction.However,the high content of total flavonoids has not been extracted from S.china.The therapeutic effects and mechanisms of total flavonoids are still unknown.In the present study,we aimed to obtain the effective parts of flavonoids in S.china,and elucidate the mechanism of action on PID.The anti-inflammatory effect was evaluated on phenol mucilage-induced pelvic inflammation in rats.This was the first time that total flavonoids in high concentration(up to 55.6%)were obtained in S.china.A total of 15 compounds were separated and identified by NMR,and the total flavonoids were detected with HPLC.TFSC caused a reduction in serum levels of IL-6 and IL-1β.These results suggested that TFSC had a significant anti-PID effect,probably due to inhibition of the inflammatory reaction.展开更多
基金The Science and Technology Department of Jiangxi Province(Grant No.20192ACB20016,20165BCB18006,20172BCB22010,GJJ201214)the National Natural Science Foundation of China(NSFC,Grant No.8150640)。
文摘As a Chinese medicinal plant,Smilax china is traditionally used in the treatment of pelvic inflammatory disease(PID).Flavonoids have been identified as the anti-inflammatory bioactive fraction.However,the high content of total flavonoids has not been extracted from S.china.The therapeutic effects and mechanisms of total flavonoids are still unknown.In the present study,we aimed to obtain the effective parts of flavonoids in S.china,and elucidate the mechanism of action on PID.The anti-inflammatory effect was evaluated on phenol mucilage-induced pelvic inflammation in rats.This was the first time that total flavonoids in high concentration(up to 55.6%)were obtained in S.china.A total of 15 compounds were separated and identified by NMR,and the total flavonoids were detected with HPLC.TFSC caused a reduction in serum levels of IL-6 and IL-1β.These results suggested that TFSC had a significant anti-PID effect,probably due to inhibition of the inflammatory reaction.
