Effect of inoculation with arbuscular mycorrhizal fungi on the degradation of DEHP in soil

The effect of inoculation with arbuscular mycorrhizal(AM) fungi( Acaulospora lavis ) on the degradation of di(2 ethylhexyl) phthalate(DEHP) in soil was studies. Cowpea plants (Pigna sinensis) were used as host plants and grown in a specially designed rhizobox. The experimental results indicated that, both in sterile and non sterile soil, mycorrhizal colonization rates were much higher in the mycorrhizal plants than in the non mycorrhizal plants. Addition of 4 mg/kg DEHP slightly affected mycorrhizal colonization, but the addition of 100 mg/kg DEHP significantly decreased mycorrhizal colonization. DEHP degradation in the mycorrhizosphere(Ms) and hyphosphere(Hs), especially in the Hs, increased after inoculation with Acaulospora lavis . It is concluded that mycorrhizal hyphae play an important role in the plant uptake, degradation and translocation of DEHP. The mechanism might be attributed to increased numbers of bacteria and actinomycetes and activity of dehydrogenase, urease and acid phosphatase in the Ms and Hs by mycorrhizal fungi.

Screening Psychrophilic Fungi of Cellulose Degradation and Characteristic of Enzyme Production

A fungus（WR-C1） decomposed cellulose was isolated from a hypothermal litter layer using Congo red medium as the preliminary screening culture medium and then using a filter as the secondary screening medium at low temperature. The experiment showed that the weight loss rate of filter paper on the 15 th days could reach 30.69%. A morphologic and ITS gene sequence analysis suggested that CF-C1 was Cladosporium. We mainly studied the effects of culture time, inoculation amount, initial p H and different sources of carbon, nitrogen and inorganic salt on the cellulase production of strain WR-C1. Under optimum cultural condition, the highest value of WR-C1 enzyme production and filter paper enzyme were 3.27 U · m L～（-1） and 0.51 U · m L～（-1）.

Straw bio-degradation by acidogenic bacteria and composite fungi

A composite microbial system, including a strain of Candida tropicalis(W3), a strain of Lactobacillus plantarm(WY3) and three strains of basidiomycete pL104, pL113 and C33, was chosen to degrade corn straw. The final pH was acid owing to the inoculation of acidogenic bacteria, and under this condition the composite fungi system could produce complex enzyme to destroy the compact structure of corn straw. The experimental results showed that the biomass of composite fungi could reach up to maximum when the pH value was 4.5. Through the bio-degradation by combining acidogenic bacteria with the composite fungi system, the cellulose, hemi-cellulose and lignin degradation rates of corn straw powder were 26.36%, 43.30% and 26.96%, respectively. And the gross crude protein content increased 60.41%. This study provided the evidence for the feasibility of developing a composite microbial system with high capability of degrading straw lignocelluloses in order to make reasonable use of straw resource and protect rural eco-environment.

Experimental Study on Decolorization and Degradation of Reactive Brilliant Red X-3B in a White Rot Fungal Biofilm Reactor

Experimental results of an azo dye(reactive brilliant red X 3B, RBR X 3B) decolorization and degradation in a white rot fungal biofilm reactor were introduced and discussed. The fungal biofilm reactor is highly potential for dye decolorization and degradation with the highest decoloring rate of 95% within 96 hours reaction time at initial pH 4.5 under high nitrogen level (HN) (24 mmol/L ammonium tartrate) condition. Experimental conditions, such as nutrient nitrogen levels in reaction mixture and initial pH, significantly affected dye decolorization and degradation. Effluents from this biofilm reactor can be well treated to meet the discharging requirements by use of chemical flocculation.RBR X 3B was first absorbed onto fungal biomass and then degraded gradually. The SH 13 fungus monopolized the biofilm throughout the experiments, though the reactor was exposed to open air for 4 months.

Investigation on Microorganisms and their Degradation Efficiency in Paper and Pulp Mill Effluent

Paper and pulp mill is a source of major pollution generating industry leaving huge amount of intensely colored effluent to the receiving end. Rapid increase of population and the increased demand for industrial establishments to meet human needs have created problems such as over exploitation of available resources, increased pollution taking place on land, air and water environment. The intention of this research paper is to identify predominant bacteria and fungi in paper and pulp mill effluent in addition to evaluate the degradation efficiency of individual isolates and combination of isolates. Treatment efficiency of individual isolates and combination of isolates are evaluated by shake flask method. Combination of Pseudomonas Alkaligenes, Bacillus subtilis along with Trichoderma reesei shows higher BOD, COD reduction of 99% and 85% respectively. As individual isolates Pseudomonas Alkaligenes show 92% BOD reduction and 77% COD reduction over other bacterial isolates and Trichoderma reesei removed 99% BOD and 80% COD respectively.

Effects of microplastic polystyrene,simulated acid rain and arbuscular mycorrhizal fungi on Trifolium repens growth and soil microbial community composition

Microplastic pollution is a global and ubiquitous environmental problem in the oceans as well as in the terrestrial environment.We examined the fate of microplastic polystyrene(MPS)beads in experimental soil in the presence and absence of symbiotic arbuscular mycorrhizal fungi(AMF)and simulated acid rain(SAR)to determine whether the combinations of these three factors altered the growth of white clover Trifolium repens.We found that MPS,SAR,or AMF added singly to soil did not alter T.repens growth or yields.In contrast,MPS and AMF together significantly reduced shoot biomass,while SAR and MPS together significantly reduced soil available phosphorus independent of AMF presence.Microplastic polystyrene,AMF,and SAR together significantly reduced soil NO_(3)^(-)-N.Arbuscular mycorrhizal fungi added singly also enriched the beneficial soil bacteria(genus Solirubrobacter),while MPS combined with AMF significantly enriched the potential plant pathogenic fungus Spiromastix.Arbuscular mycorrhizal fungi inoculation with MPS increased the abundance of soil hydrocarbon degraders independent of the presence of SAR.In addition,the abundance of soil nitrate reducers was increased by MPS,especially in the presence of AMF and SAR.Moreover,SAR alone increased the abundance of soil pathogens within the fungal community including antibiotic producers.These findings indicate that the coexistence of MPS,SAR,and AMF may exacerbate the adverse effects of MPS on soil and plant health.

Interactions between Two Fungi Strains during Litter Decomposition through a Microcosm Experiment: Different Degradative Enzyme Activities

Fungi are the key agents in litter decomposition in forest ecosystems. However, the specific roles of the interactions between different fungal species during litter decomposition process are unclear. To evaluate the interactions, two fungi strains with significantly different morphs were isolated from the soils of Quercus acutissima forest and Pinus massoniana forest, and inoculated in the litter powder of Quercus acutissima leaves and Pinus massoniana needles with grown separately and in coexistence equally through a microcosm experiment. The enzyme activities were determined as a proxy for microbial activities. The results showed that the degradative enzymes involved in litter decomposition showed varying dynamics pattern during the incubation period. The interactions between the two fungi strains are synergism, and benefit to each other according to enzyme activities, suggesting that a fungi strain growth was accelerated by the presence of other fungi strain during litter decomposition process. However, the interactions of the two fungi strains were bilateral antagonism inoculated in the litter powder of Quercus acutissima leaves according to cellobiohydrolase activities. The synergism, despite bilateral antagonism in an exceptional case, may be an important factor controlling the fungal colonization and growth on litter substrate. The results implied that more fungal species may accelerate litter decomposition rates due to their mutual cooperation.

退化高寒草甸土壤真菌群落水平分布格局及驱动因子

为探讨退化高寒草甸土壤真菌群落水平分布格局及驱动因子,本研究采用嵌套式取样方法在1000 m×1000 m的中度退化草甸区域内,综合分析了土壤真菌群落在不同取样距离上物种多样性和功能组成的变化规律及驱动土壤真菌群落分布的主要环境因子。结果表明:在不同取样距离上,土壤真菌的α多样性和群落结构存在显著的差异,退化草甸环境异质性可导致真菌群落的变化。FunGuil功能预测表明草甸退化使得腐生型和病理性土壤真菌增加,草甸退化过程中地上植被群落的改变可导致土壤真菌营养型的变化。此外,土壤营养成分是驱动退化草甸土壤真菌群落水平分布格局的主要环境因子。因此,微生物多样性空间分布受控于不同的环境因素,而土壤营养成分的改变容易引起退化草地生态系统中微生物的空间分布变化。

有效降解农膜的真菌筛选与复合菌群的构建

为解决农用地膜在土壤中难降解的问题,从土壤中筛选菌株且对不同农膜材料降解的研究。本研究从黑色聚乙烯(PE)地膜、白色(PE)地膜、聚乙烯和聚己二酸/对苯二甲酸丁二酯(PBAT)降解地膜、聚甲基乙撑碳酸酯(PPC)降解地膜等4种不同材料覆盖下的土壤中分离筛选两株有效降解农膜的真菌,经过鉴定得到菌株Penicillium citrinum(JW1)和Aspergillus costaricensis(JW2)。将JW1和JW2菌株进行复合,观察农膜降解前后表面微观特征的改变情况。结果表明,四种覆膜材料在同时接种JW1、JW2菌株后,均出现不同程度的降解,在接种复合菌群降解30 d后,PPC降解地膜、PBAT降解地膜、黑色PE地膜和透明PE地膜的失重率分别为23.78%、14.73%、22.78%和17.55%,接种复合菌群对PPC地膜的降解效果显著高于其它三种地膜(P<0.05)。本试验旨在筛选出有效降解农膜的真菌菌株,以期为农膜的微生物降解技术提供技术支持。

三江源区高寒草原退化对不同生长期土壤真菌群落的影响

土壤真菌群落在草地生态系统物质循环过程中扮演着重要角色,但草地退化对不同生长期土壤真菌群落的影响尚不清楚。本研究以三江源高寒草原为研究对象,通过野外调查和高通量测序技术,探究草原退化对植物不同生长期土壤真菌群落特征的影响及其驱动因素。结果表明,三江源区高寒草原不同生长期土壤真菌群落优势菌门均为子囊菌门和担子菌门,草原退化显著降低了优势菌门的相对丰度;在不同生长期土壤真菌群落多样性存在显著差异(P<0.05),表现为生长季初期较低,末期则较高;草原退化对土壤真菌群落多样性影响不显著(P>0.05),但显著改变了不同生长期内土壤真菌群落结构(P<0.05);相比于原生草原,退化草原土壤真菌群落结构与植物群落特征表现出更强的相关关系,植物群落生物量和土壤有机质含量是显著影响退化草原土壤真菌群落结构的主要因子(P<0.05)。研究结果表明,草原退化改变了土壤真菌群落结构,增强了土壤真菌群落的资源限制。因此,针对植被群落的修复将有利于退化高寒草原土壤真菌群落的恢复。

Evaluation of ruminal methane and ammonia formation and microbiota composition as affected by supplements based on mixtures of tannins and essential oils using Rusitec

Background Dietary supplements based on tannin extracts or essential oil compounds(EOC)have been repeatedly reported as a promising feeding strategy to reduce the environmental impact of ruminant husbandry.A previous batch culture screening of various supplements identified selected mixtures with an enhanced potential to mitigate ruminal methane and ammonia formation.Among these,Q-2(named after quebracho extract and EOC blend 2,composed of carvacrol,thymol,and eugenol)and C-10(chestnut extract and EOC blend 10,consisting of oregano and thyme essential oils and limonene)have been investigated in detail in the present study with the semi-continuous rumen simulation technique(Rusitec)in three independent runs.For this purpose,Q-2 and C-10,dosed according to the previous study,were compared with a non-supplemented diet(negative control,NC)and with one supplemented with the commercial EOC-based Agolin^(R) Ruminant(positive control,PC).Results From d 5 to 10 of fermentation incubation liquid was collected and analysed for pH,ammonia,protozoa count,and gas composition.Feed residues were collected for the determination of ruminal degradability.On d 10,samples of incubation liquid were also characterised for bacterial,archaeal and fungal communities by high-throughput sequencing of 16S rRNA and 26S ribosomal large subunit gene amplicons.Regardless of the duration of the fermentation period,Q-2 and C-10 were similarly efficient as PC in mitigating either ammonia(-37%by Q-2,-34%by PC)or methane formation(-12%by C-10,-12%by PC).The PC was also responsible for lower feed degradability and bacterial and fungal richness,whereas Q-2 and C-10 effects,particularly on microbiome diversities,were limited compared to NC.Conclusions All additives showed the potential to mitigate methane or ammonia formation,or both,in vitro over a period of 10 d.However,several differences occurred between PC and Q-2/C-10,indicating different mechanisms of action.The pronounced defaunation caused by PC and its suggested consequences apparently determined at least part of the mitigant effects.Although the depressive effect on NDF degradability caused by Q-2 and C-10 might partially explain their mitigation properties,their mechanisms of action remain mostly to be elucidated.

防火林带景观树种枯落物的高效降解真菌筛选及效果评估

[目的]为森林防火和枯枝落叶的循环利用,筛选和优化菌株,并应用于防火林带景观树种枯枝落叶的降解。[方法]采用树种枯枝落叶叶片条降解法、降解失重法筛选出强降解菌株,再比较强降解菌株混合联用的降解失重效果,选出强降解菌株的混合联用组合,并分析其对枯枝落叶的降解效果和作用。[结果]通过比较11菌株对12种防火林带景观树种枯枝落叶的降解效果,筛选出绵皮卧孔菌(Pp)、康宁木霉(Tk_(1))和云芝(Pv)等3株强降解菌株;3菌株单用及联用对檫树、红叶石楠和木荷枯枝落叶降解失重率分别为54.3%±2.3%和62.1%±3.3%。[结论]筛选出的3菌株单用及联用组合,Pp+Pv、Pv+Tk_(1)和Pp+Tk_(1)对防火林带景观树种枯枝落叶有明显的降解效果,可用于苏南丘陵山区的防火和枯枝落叶处理工作。

光臀八齿小蠹共生微生物群落组成及其功能基因注释

【目的】揭示光臀八齿小蠹Ips nitidus共生微生物群落组成和功能特征。【方法】对来自中国西北部青海省麦秀自然保护区的光臀八齿小蠹进行肠道和其他组织共生真菌和细菌宏基因组学和相关性网络分析,比较光臀八齿小蠹肠道和其他组织共生真菌及细菌群落组成,分析微生物群落间的相互关系,并采用非冗余蛋白序列比对方法注释共生微生物功能基因。【结果】共发现光臀八齿小蠹肠道和其他组织共生微生物3520种,真菌和细菌的物种数最高,分别占全部物种数的23.21%和69.01%,平均相对丰度分别为9.69%和13.79%。其他组织中共生真菌白粉菌目(Erysiphales)相对丰度和物种多样性较肠道中的高,而其他组织中酵母目(Saccharomycetales)相对丰度和物种多样性较肠道中的低;其他组织中长喙壳类真菌(ophiostomatoid fungi)多样性(6属16种)较在肠道中的(5属13种)高,在其他组织和肠道中的平均相对丰度分别为0.016%和0.013%。其他组织中肠杆菌目(Enterobacterales)相对丰度和物种多样性高于肠道中的。肠杆菌属Enterobacter、白粉菌属Erysiphe、丛枝菌根属Rhizophagus和沃尔巴克氏菌属Wolbachia均为肠道和其他组织的核心菌属。真菌和细菌的某些群落在肠道和其他组织间表现出相反的相互关系,表明光臀八齿小蠹共生菌间可能存在复杂的相互作用。分别鉴定到共生真菌和细菌功能基因7010和6483个,细菌中异源性物质生物降解和代谢相关功能基因数量远高于真菌的;mRNA监测通路、丙酸代谢和泛素介导的蛋白质水解等11条通路在肠道中较在其他组织中具有更多的差异基因;对于柠檬烯降解和甾醇合成相关功能基因的注释结果表明,细菌对柠檬烯较真菌具有明显的降解能力,而真菌在甾醇生物合成中较细菌发挥更重要的促进作用;74个长喙壳类真菌功能基因主要参与到氧化磷酸化、代谢通路和氨基酸代谢,且长喙壳类真菌在其他组织中多样性更高,主要分布于Ophiostoma等。【结论】光臀八齿小蠹肠道和其他组织中共生微生物的群落组成和多样性以及功能上存在一定的差异性,肠杆菌属、白粉菌属、丛枝菌根属和沃尔巴克氏菌属是光臀八齿小蠹共生微生物群落中最重要的核心菌群,可能在群落构建中发挥着重要的作用。

微生物菌剂对秸秆降解及秸秆中微生物变化规律的影响

为明确不同微生物菌剂对秸秆降解率及秸秆降解过程中秸秆周围微生物变化规律的影响,在温室大棚内进行不同微生物菌剂降解秸秆试验。以玉米秸秆为基质,设玉米秸秆(对照组)、玉米秸秆+哈茨木霉(Trichoderma harzianum)(处理1)、玉米秸秆+哈茨木霉+地衣芽胞杆菌(Bacillus licheniformis)(处理2)三个处理。研究结果显示,单独用哈茨木霉处理可显著提高秸秆降解率,前期作用尤其明显;在秸秆降解的前30 d,秸秆降解率与秸秆中可培养真菌、细菌、放线菌呈显著正相关。不同微生物菌剂对秸秆中可培养微生物数量变化有显著影响,单独接种哈茨木霉后,秸秆中可培养真菌、细菌、放线菌数在前30 d显著高于对照组和混合菌剂处理,不同处理可培养活菌数均在90~120 d达到峰值,然后开始下降。研究结果表明,不同微生物菌剂对秸秆降解有显著影响,单独用哈茨木霉处理可显著提高秸秆降解率;不同处理对秸秆中可培养真菌、细菌、放线菌的影响有显著差异。可为秸秆降解与微生物相关性研究提供参考。

食用菌菌渣高效降解菌的筛选及主菌株产酶环境优化

为筛选出高效降解食用菌废弃菌渣的复合菌系,有效促进菌渣的降解速率,明确复合菌系主要功能菌株最佳发酵环境。利用富集培养、限性继代培养和低温逐代驯化技术对205份采集的菌源材料进行筛选,通过对纤维素酶活性的测定,选取最佳菌源提取物料。同时采用划线分离培养,分离主要功能菌株,对菌株的产酶条件进行优化。结果表明,从205份菌源提取物质中分离纯化的1个具有较强的纤维素降解能力的材料,发现该菌属于曲霉属,产酶较高的主菌株是聚多曲霉(Aspergillus sydowii),对该菌株的产酶环境优化发现,产半纤维素酶和纤维素酶的最优环境是菌渣35 g·L^(-1)、硫酸铵3.5 g·L^(-1)、吐温-80为1.4 mL·L^(-1)、KH_(2)PO_(4)1.0 g·L^(-1)、MgSO_(4)·7H_(2)O 1.0 g·L^(-1)、NaCl 0.5 g·L^(-1)、FeSO_(4)·7H_(2)O 0.1 g·L^(-1),培养时间为15 d。最终明确了复合菌系中主要功能菌株的菌株类别及其发酵环境,可为废弃菌渣资源的快速降解提供宝贵资源,并为菌渣资源肥向利用提供技术依据。

不同丛枝菌根真菌接种对3种草地植物生长特性的影响

为筛选出适用于农牧交错带沙荒地的植物和丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)的优势组合,本研究选取沙荒地常见的3种植物,紫花苜蓿(Medicago satuva L.)、无芒雀麦(Bromus inermis Leyss)和苇状羊茅(Festuca arundinacea Schreb.)作为试验材料,设置接种幼套球囊霉(Glomus etunicatum,GE)、摩西球囊霉(Glomus mosseae,GM)、根内球囊霉(Glomusntraradices,GI)和不接种(CK)处理。结果表明:根内球囊霉(GI)接种紫花苜蓿、无芒雀麦和苇状羊茅接种的侵染率最高,其次是摩西球囊霉(GM),幼套球囊霉(GE)。相关分析也表明,AMF侵染率与植物株高、生物量和分蘖(枝)数呈极显著正相关。综上,接种AMF能显著提高3种草地植物的株高、生物量和分蘖(枝)数,促进植物的生长。GI接种无芒雀麦的组合适用于农牧交错带荒滩地,为探索利用微生物高效快速的进行沙荒地植被恢复提供技术参考。

桑寄生种子高产木质纤维素降解酶内生真菌的筛选与鉴定

【目的】筛选、分离、鉴定桑寄生种子高产木质纤维素降解酶内生真菌,为获取降解能力较强的木质纤维素降解酶及实现桑寄生人工栽培提供技术支撑。【方法】以桑寄生种子为研究对象,采用常规组织分离法分离其内生真菌,以麦麸为木质纤维生物物质底物,采用ABTS法、藜芦醛法和苯酚红法及纤维素酶(CL)活性检测试剂盒分别测定其漆酶、木质素过氧化物酶、锰过氧化物酶和纤维素降解酶活性,筛选降解木质纤维素能力较强的菌株,并根据形态特征和系统进化树分析确定其分类地位。【结果】从57株桑寄生种子内生真菌中筛选出4株高产木质纤维素降解酶菌株(15H3、2013、106和10y11)。其中,培养11 d时菌株15H3的木质素过氧化物酶活性和菌株2013的锰过氧化物酶活性达高峰,分别为7.14和11.69 U/mL;培养9 d时菌株106的漆酶活性、菌株2013的纤维素酶活性和菌株10y11的锰过氧化物酶活性最高,分别为57.98、0.32和8.56 U/mL。经形态学观察和分子生物学鉴定,15H3和2013分别为拟盘多毛孢属(Pestalotiopsis arceuthobii)和垫壳孢属(Coniella pseudogranati)菌株,106和10y11为镰孢属(Fusarium incarnatum)菌株。【结论】从桑寄生种子中能筛选鉴定出高产木质纤维素降解酶内生真菌拟盘多毛孢属、垫壳孢属和镰孢属,可为桑寄生侵入寄主细胞壁降解机制研究提供理论依据,为桑寄生的繁育提供数据支持。

丝状真菌降解拟除虫菊酯类农药和3-苯氧基苯甲酸研究进展

拟除虫菊酯(pyrethroids,PYRs)类农药由于低毒、高效被广泛使用,其残留造成的环境和食品安全问题日益严重,3-苯氧基苯甲酸(3-phenoxybenzoic acid,3-PBA)是PYRs的主要中间降解产物之一,比母体农药更稳定,毒性更强,其残留进一步加重了环境和农产品污染。近年来,丝状真菌对PYRs和3-PBA降解的相关研究陆续被报道,其主要集中在降解菌株筛选、降解特性、降解途径及降解酶定位等方面,对于降解过程中涉及的关键酶及其编码基因少见报道。随着测序技术的发展,分子生物学技术为挖掘丝状真菌降解酶及编码基因提供了新途径。本文简述了PYRs和3-PBA的基本性质、残留现状及危害,综述了降解PYRs和3-PBA的丝状真菌、降解途径及降解酶,并对降解酶及其编码基因筛选和验证方法进行了展望,以期为揭示丝状真菌对PYRs及3-PBA的降解途径和降解机制提供理论依据,为消减环境及农产品中残留农药提供参考。

丝状真菌Podospora anserina AA11家族裂解多糖单加氧酶基因的鉴定和功能研究

辅助活性蛋白家族(auxiliary activity family,AA family)中的裂解多糖单加氧酶(lytic polysaccharide monooxygenase,LPMO)能催化纤维素、几丁质和淀粉等多种难降解碳水化合物的氧化解聚。尽管目前对LPMO的酶学研究较多,但对LPMO基因失活的研究却鲜有报道。本研究利用同源重组方法定点敲除丝状真菌Podospora anserina中AA11家族的5个LPMO基因PaLPMO11A(Pa_4_4790)、PaLPMO11B(Pa_1_5310)、PaLPMO11C(Pa_2_7840)、PaLPMO11D(Pa_2_8610)和PaLPMO11E(Pa_3_9420),分别构建了单突变体ΔPaLPMO11A(ΔA)、ΔPaLPMO11B(ΔB)、ΔPaLPMO11C(ΔC)、ΔPaLPMO11D(ΔD)和ΔPaLPMO11E(ΔE),然后通过遗传杂交构建所有多基因突变体。通过在不同碳源培养基上的表型分析、DAB和NBT染色以及纤维素酶活测定分析野生型菌株与突变型菌株在生长速率、有性生殖、氧化应激和纤维素降解能力等方面的差异,揭示LPMO11基因在P.anserina菌株的生长发育和木质纤维素降解过程中的作用。实验结果表明,在不同纤维素碳源上,ΔBΔCΔE、ΔAΔBΔCΔE、ΔAΔCΔDΔE和ΔAΔBΔCΔDΔE突变型菌株的有性生殖能力降低,其余突变型菌株的孢子萌发效率、生长速率和生殖能力几乎没有差异。PaLPMO11家族5个基因的同时缺失,会导致菌株利用各种碳源的能力明显降低、生长速率降低、孢子萌发率降低、子实体数减少、部分子实体发育异常、寿命缩短和降解纤维素的能力显著下降,但仍有野生型45%以上的总纤维素酶活力。上述结果表明,LPMO11基因可能参与P.anserina的生长发育、有性生殖、衰老和纤维素降解过程。本研究为系统阐述丝状真菌P.anserina中木质纤维素降解的调控机制提供参考。