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Extraction and purification of TGFβ and its effect on the induction of apoptosis of hepatocytes 被引量:15
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作者 Xiao-Hui Si Lian-Jun Yang Research Institute of Stomatology,the Ninth People’s Hospital,Shanghai Second Medical University,Shanghai 200011,ChinaDepartment of Pathology,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第4期527-531,共5页
AIM To extract and purify the transforming growth factor β (TGF β), and to demonstrate its biological activity in vivo and induction of apoptosis of hepatocytes in vitro.METHODS TGF β was isolated from fresh bovine... AIM To extract and purify the transforming growth factor β (TGF β), and to demonstrate its biological activity in vivo and induction of apoptosis of hepatocytes in vitro.METHODS TGF β was isolated from fresh bovine platelets by acid/ethanol extraction method and purified with ion exchange and gel chromatography. The extracted TGF β was injected subcutaneously to mice, and its biological activity in vivo was observed 72 hfs post-injection by HE staining. The morphological changes were observed by HE staining and the occurrence of apoptosis was detected by TUNEL method after the human normal hepatic cell line QZG was treated with 8 μg@L 1 TGFβ for 12 hrs in vitro.RESULTS The molecular mass 25 ku TGF β protein was successfully extracted. It was able to induce localized granulation tissue formation in vivo. TGF β-treated hepatocytes showed obvious apoptotic morphological changes, including the pyknosis and dense-stained nuclei and cytoplasm, the fragmentary, annular or crescent nuclei, and the "bubbling" cytoplasm. Moreover, its apoptotic rate was significantly higher than that of the control group (P<0.05).CONCLUSION Biological active TGF β protein is extracted and purified successfully from bovine platelets, and it is able to induce the apoptosis of hepatocytes. 展开更多
关键词 TRANSFORMING GROWTH FACTOR beta/isolation & purification TRANSFORMING GROWTH FACTOR beta/pharmacology liver/cytology APOPTOSIS
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TLC法鉴定皮肤及肌肉组织中的青霉素 被引量:1
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作者 卢英强 宣兆艳 +5 位作者 崔佰君 徐晶华 祁柏宇 刘莉 张林庆 于广池 《吉林大学学报(医学版)》 CAS CSCD 北大核心 2003年第6期780-781,共2页
目的 :建立一种快速鉴定动物及人皮肤或肌肉组织中青霉素的方法。方法 :利用薄层层析( TLC)法将动物模型及人体皮肤、肌肉组织中青霉素分离提纯后进行鉴定。结果 :从实验动物及人皮肤或肌肉组织中分离提纯青霉素获得的色斑与青霉素标准... 目的 :建立一种快速鉴定动物及人皮肤或肌肉组织中青霉素的方法。方法 :利用薄层层析( TLC)法将动物模型及人体皮肤、肌肉组织中青霉素分离提纯后进行鉴定。结果 :从实验动物及人皮肤或肌肉组织中分离提纯青霉素获得的色斑与青霉素标准品色斑和 Rf值完全相同 ,层析色谱清晰 ,色斑鲜明 ,可在正常环境中保存 1 92 h以上。结论 展开更多
关键词 色谱法 薄层 青霉素类 分离 提纯 副作用 药物过敏 法医学 司法鉴定
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新的分离纯化青霉素酰化酶方法的研究 被引量:5
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作者 孙万儒 《生物工程学报》 CAS CSCD 北大核心 1994年第2期178-184,共7页
按0.6%(w/w)的比例将皂土加到青霉素酰化酶发酵上清液中,可将酶100%吸附,而吸附的蛋白质仅占发酵上清液中的10%左右。吸附时的pH和无机盐对酶的吸附影响不大。使用不同pH和种类的缓冲液洗涤皂土-酶复合物,不能将酶洗脱,但可洗脱15%... 按0.6%(w/w)的比例将皂土加到青霉素酰化酶发酵上清液中,可将酶100%吸附,而吸附的蛋白质仅占发酵上清液中的10%左右。吸附时的pH和无机盐对酶的吸附影响不大。使用不同pH和种类的缓冲液洗涤皂土-酶复合物,不能将酶洗脱,但可洗脱15%左右吸附的杂蛋白。使用含10%以上的PEG和NaCl的磷酸缓冲液可将酶全部洗脱,酶纯化25倍,浓缩6倍左右。此法特点是简便,酶活力收率高,可在常温下操作,也可直接从未除菌体的发酵液中提取酶,具有工业应用价值。 展开更多
关键词 青霉素酰化酶 皂土 吸附 分离
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改良酸测琼脂板法检测金黄色葡萄球菌的青霉素酶
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作者 陈惠青 《湖南医科大学学报》 CSCD 1990年第2期198-200,共3页
用改良酸测琼脂板法检测了71株金黄色葡萄球菌的青霉素酶,并与Park酸测琼脂板法作了对比。结果二者完全一致,但改良法结果更为清晰易辨。与液体倍比稀释法所测最小抑菌浓度(MIC)结果也完全相符。实验证实本法为一准确、可靠、价廉、快... 用改良酸测琼脂板法检测了71株金黄色葡萄球菌的青霉素酶,并与Park酸测琼脂板法作了对比。结果二者完全一致,但改良法结果更为清晰易辨。与液体倍比稀释法所测最小抑菌浓度(MIC)结果也完全相符。实验证实本法为一准确、可靠、价廉、快速的检测细菌青霉素酶的方法。 展开更多
关键词 葡萄球菌 青霉素酶 分离 提纯
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Studies of Coltivirus in China 被引量:8
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作者 TAOSan-ju CHENBo-quan 《Chinese Medical Journal》 SCIE CAS CSCD 2005年第7期581-586,共6页
Objective The purpose of this article is to review the developments of studies of Coltivirus in ChinaData sources The data used in this review was obtained mainly from the studies of Coltivirus reported from 1990 to 2... Objective The purpose of this article is to review the developments of studies of Coltivirus in ChinaData sources The data used in this review was obtained mainly from the studies of Coltivirus reported from 1990 to 2003 in China.Study selection Relevant articles on studies of Coltivirus in domestic and foreign literature were selected.Data extraction Data were maily extracted from the articles which are listed in the reference section of this review.Results Many Coltiviruses have been isolated not only from blood samples of patients with unknown fever or from cerebrospinal fluid of patients with encephalitis in Xishuangbanna area in Yunnan province, but also from mosquitoes collected in many areas in China. In some patients diagnosed as Japanese encephalitis or unknown fever, an increase of Coltivirus IgG antibody of fourfold, or more, has been detected using ELISA. Similarly, Coltivirus IgM antibody was positive in some patients with Japanese encephalitis or viral encephalitis. From most Chinese patients, except the northeastern, the isolates of Coltiviruses belong to subgroup B2, according to RT-PCR amplification of the ninth and twelfth segments of the isolates and sequence analysis of their amplicons. Some biological properties of Chinese Coltiviruses isolates are different from that of North American Coltiviruses.Conclusions The isolates of Coltiviruses from Chinese patients are one of the common agents causing viral encephalitis and unknown fever in summer-autumn season. It might be an important public health problem due to its high isolation rate and wide distribution in China. Mosquito is the main transmission vector of the virus. 展开更多
关键词 Coltivirus isolation & purification serologic tests GENOTYPE
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