A total of 30 Nubian adult goats were randomly divided into three groups, with 10 goats in each group. The goats in different groups were fed with penn- isetum, purpureum Schum cv. Taiwan, [P. Purpureum. cv. Mort x （...A total of 30 Nubian adult goats were randomly divided into three groups, with 10 goats in each group. The goats in different groups were fed with penn- isetum, purpureum Schum cv. Taiwan, [P. Purpureum. cv. Mort x （P. americanum x P. purureum） cv. Guimu No. 1] and P. purpureum cv. Southern China respectively. In the 60-days-test, the nutritional value of the forages, the intake, growth and economic benefits of the goats were determined and analyzed. The resuits showed that, the weight gain of the goats feeding with P. purpureum Schum cv. Taiwan was remarkable, with the daily weight gain reaching （144.44 ± 17.80） g, which were 32.37% and 88.48% higher than the control groups. The feed reward in P. purpureum Schum cv. Taiwan group was the highest, with feed conversion rate of 19. 10：1 and the significant economic benefits. On the whole, P. purpureum Schum cv. Taiwan with a high nutritional value and a good palatability was a kind of excellent forages for goats.展开更多
[Objectives]The paper was to study the cutting propagation technique of Pennisetum purpureum Schum and to provide a technical reference for establishing an efficient cutting propagation method.[Methods]Six treatments ...[Objectives]The paper was to study the cutting propagation technique of Pennisetum purpureum Schum and to provide a technical reference for establishing an efficient cutting propagation method.[Methods]Six treatments were set up using P.purpureum cv.Guiminyin and P.purpureum cv.Guimu-1 as test materials,including 1-node oblique insertion,1-node oblique insertion+rooting powder,1-node transverse burial,2-node oblique insertion,2-node oblique insertion+rooting powder and 2-node transverse burial.The following indices were observed and determined for P.purpureum cuttings:emergence rate,rooting rate,root number,longest root length,fresh root weight,plant height,number of tillers,number of leaves,and fresh stem and leaf weight.[Results]In the 2-node cutting+rooting powder treatment,Guiminyin and Guimu-1 exhibited the highest survival rate,root growth indices,and stem and leaf growth indices,with the emergence rates of 94.29%and 90.26%,respectively.The 2-node cutting treatment followed closely behind,while the 1-node cutting treatment had the lowest indices.Under the same treatment,Guimuyin exhibited higher mean values for plant height,number of leaves,fresh stem and leaf weight,longest root length,and fresh root weight compared to Guimu-1.However,it had lower mean number of tillers,and emergence rate and rooting rate of the 1-node cutting treatment compared to Guimu-1.[Conclusions]The P.purpureum cuttings thrived in the 2-node cutting+rooting powder treatment,and the overall cutting effect of Guiminyin was superior to that of Guimu-1.展开更多
[Objective] This study was to investigate the effect of atrazine stress on the growth of Pennisetum hydridum. [Method] Pot experiments were conducted to study the effects of atrazine stress （20, 50, 100, 200, 500 mg...[Objective] This study was to investigate the effect of atrazine stress on the growth of Pennisetum hydridum. [Method] Pot experiments were conducted to study the effects of atrazine stress （20, 50, 100, 200, 500 mg/kg） on plant height, biomass, root-shoot ratio and chlorophyll content of P. hydridum. [Results] Low level of atrazine stress （20 and 50 mg/kg） showed no significant effects on plant height and biomass of P. hydridum. Moderate level of atrazine stress （100 and 200 mg/kg） did not show significant effect on plant height, but did on the biomass of P. hydridum. Biomass of P. hydridum stressed by 100 and 200 mg/kg of atrazine was decreased by 34.1% and 36.4% compared with control, respectively. High level of atrazine stress （500 mg/kg） brought significant decrease in plant height（by 40.6%） and biomass（20.0%） of P. hydridum. All levels of atrazine stress showed no significant effects on root-shoot ratio and chlorophyll content of P. hydridum. [Conclusion] Pennisetum hydridum has strong tolerance to atrazine stress.展开更多
[Objective] The aim was to study pollen morphology of Pennisetum native species.[Method] We carried out the observation by optical microscope and scanning electron microscope on six materials of pollen grains includin...[Objective] The aim was to study pollen morphology of Pennisetum native species.[Method] We carried out the observation by optical microscope and scanning electron microscope on six materials of pollen grains including three species and three transmogrifications from one variant of Pennisetum native species.[Result] The pollen grains were globose or subglobose.Germination aperture which located at distal side was singular and its circumambience was thickened.Pollen wall which had two layers was thin and easy to be crimpled.There were obvious differences between ektexine ornamentations as well as particle sizes.[Conclusion] The question of low seed-setting rate of Pennisetum native species had relations with the withered pollen morphology to some extent.The differences between pollen morphology as well as ektexine ornamentations accorded with the division of species and variants from Pennisetum.In addition,the relationship between pollen morphology and seed-setting rate provided references to introduce good grass seed and choose breeding parent materials of Pennisetum.展开更多
[Objective] The aim was to clone the cDNA and DNA sequences of the CCR （Cinnamoyl-CoA reductase） gene which involves in lignin biosynthesis, from Pennisetum purpureum, and to make comprehensive analysis on these seq...[Objective] The aim was to clone the cDNA and DNA sequences of the CCR （Cinnamoyl-CoA reductase） gene which involves in lignin biosynthesis, from Pennisetum purpureum, and to make comprehensive analysis on these sequences. [Method] CCR sequences were cloned from P. purpureum by using conventional RT-PCR and RACE （Rapid Amplification of cDNA Ends） methods; and the bioinformatic analyses of the CCR were conducted by means of NCBI, ProtParam ProtScale, TMHMM, TargetP, SignalP, Pfam20.0, Prosite, Swiss-Model, ClustalW2, DNAman, DNAstar and MEGA5. [Result] The cloned PpCCR （P. purpureum CCR） cDNA sequence was 1 316 bp, including a 1 110 bp ORF and 206 bp 3’-UTR. The cloned DNA sequence from PpCCR was 6 133 bp in full-length, containing five exons and four introns. Bioinformatic analysis indicated that PpCCR encoded a polypeptide of 369 amino acids, the secondary structure of which was primarily composed of random coil and α-helix, belonging to NAD-dependent epimerase/dehydratase family, and its co-factor binding sites and substrate binding sites were highly conserved. [Conclusion] DNA and cDNA sequences of CCR gene were obtained from P. purpureum, which had the typical characteristics of other homologous genes. The obtained bioinformatic data provided theoretical references for the further analysis of CCR and better application of P. purpureum in the future.展开更多
基金Supported by Provincial Bureau of Science and Technology Project(2010001)Province agricultural science and technology achievements transformation projects(1346004-4)
文摘A total of 30 Nubian adult goats were randomly divided into three groups, with 10 goats in each group. The goats in different groups were fed with penn- isetum, purpureum Schum cv. Taiwan, [P. Purpureum. cv. Mort x （P. americanum x P. purureum） cv. Guimu No. 1] and P. purpureum cv. Southern China respectively. In the 60-days-test, the nutritional value of the forages, the intake, growth and economic benefits of the goats were determined and analyzed. The resuits showed that, the weight gain of the goats feeding with P. purpureum Schum cv. Taiwan was remarkable, with the daily weight gain reaching （144.44 ± 17.80） g, which were 32.37% and 88.48% higher than the control groups. The feed reward in P. purpureum Schum cv. Taiwan group was the highest, with feed conversion rate of 19. 10：1 and the significant economic benefits. On the whole, P. purpureum Schum cv. Taiwan with a high nutritional value and a good palatability was a kind of excellent forages for goats.
基金the Fundamental Research Funds for Sichuan Animal Science Academy(SASA202106)the Modern Agricultural Industry System Sichuan Mutton Sheep Innov Team(sccxtd-2023-14)Sichuan Province Breeding Research Grant(2021YFYZ0013).
文摘[Objectives]The paper was to study the cutting propagation technique of Pennisetum purpureum Schum and to provide a technical reference for establishing an efficient cutting propagation method.[Methods]Six treatments were set up using P.purpureum cv.Guiminyin and P.purpureum cv.Guimu-1 as test materials,including 1-node oblique insertion,1-node oblique insertion+rooting powder,1-node transverse burial,2-node oblique insertion,2-node oblique insertion+rooting powder and 2-node transverse burial.The following indices were observed and determined for P.purpureum cuttings:emergence rate,rooting rate,root number,longest root length,fresh root weight,plant height,number of tillers,number of leaves,and fresh stem and leaf weight.[Results]In the 2-node cutting+rooting powder treatment,Guiminyin and Guimu-1 exhibited the highest survival rate,root growth indices,and stem and leaf growth indices,with the emergence rates of 94.29%and 90.26%,respectively.The 2-node cutting treatment followed closely behind,while the 1-node cutting treatment had the lowest indices.Under the same treatment,Guimuyin exhibited higher mean values for plant height,number of leaves,fresh stem and leaf weight,longest root length,and fresh root weight compared to Guimu-1.However,it had lower mean number of tillers,and emergence rate and rooting rate of the 1-node cutting treatment compared to Guimu-1.[Conclusions]The P.purpureum cuttings thrived in the 2-node cutting+rooting powder treatment,and the overall cutting effect of Guiminyin was superior to that of Guimu-1.
基金Supported by Natural Science Foundation of Yunnan Province(2010CD058)~~
文摘[Objective] This study was to investigate the effect of atrazine stress on the growth of Pennisetum hydridum. [Method] Pot experiments were conducted to study the effects of atrazine stress （20, 50, 100, 200, 500 mg/kg） on plant height, biomass, root-shoot ratio and chlorophyll content of P. hydridum. [Results] Low level of atrazine stress （20 and 50 mg/kg） showed no significant effects on plant height and biomass of P. hydridum. Moderate level of atrazine stress （100 and 200 mg/kg） did not show significant effect on plant height, but did on the biomass of P. hydridum. Biomass of P. hydridum stressed by 100 and 200 mg/kg of atrazine was decreased by 34.1% and 36.4% compared with control, respectively. High level of atrazine stress （500 mg/kg） brought significant decrease in plant height（by 40.6%） and biomass（20.0%） of P. hydridum. All levels of atrazine stress showed no significant effects on root-shoot ratio and chlorophyll content of P. hydridum. [Conclusion] Pennisetum hydridum has strong tolerance to atrazine stress.
基金Supported by Special Fund for Basic Research Operating Expenses of Nonprofit Research Institutions at the Central Level(BRF090202)~~
文摘[Objective] The aim was to study pollen morphology of Pennisetum native species.[Method] We carried out the observation by optical microscope and scanning electron microscope on six materials of pollen grains including three species and three transmogrifications from one variant of Pennisetum native species.[Result] The pollen grains were globose or subglobose.Germination aperture which located at distal side was singular and its circumambience was thickened.Pollen wall which had two layers was thin and easy to be crimpled.There were obvious differences between ektexine ornamentations as well as particle sizes.[Conclusion] The question of low seed-setting rate of Pennisetum native species had relations with the withered pollen morphology to some extent.The differences between pollen morphology as well as ektexine ornamentations accorded with the division of species and variants from Pennisetum.In addition,the relationship between pollen morphology and seed-setting rate provided references to introduce good grass seed and choose breeding parent materials of Pennisetum.
基金Supported by the National Natural Science Foundation of China(30972138)the Guangdong Natural Science Foundation(9451064201003804)~~
文摘[Objective] The aim was to clone the cDNA and DNA sequences of the CCR （Cinnamoyl-CoA reductase） gene which involves in lignin biosynthesis, from Pennisetum purpureum, and to make comprehensive analysis on these sequences. [Method] CCR sequences were cloned from P. purpureum by using conventional RT-PCR and RACE （Rapid Amplification of cDNA Ends） methods; and the bioinformatic analyses of the CCR were conducted by means of NCBI, ProtParam ProtScale, TMHMM, TargetP, SignalP, Pfam20.0, Prosite, Swiss-Model, ClustalW2, DNAman, DNAstar and MEGA5. [Result] The cloned PpCCR （P. purpureum CCR） cDNA sequence was 1 316 bp, including a 1 110 bp ORF and 206 bp 3’-UTR. The cloned DNA sequence from PpCCR was 6 133 bp in full-length, containing five exons and four introns. Bioinformatic analysis indicated that PpCCR encoded a polypeptide of 369 amino acids, the secondary structure of which was primarily composed of random coil and α-helix, belonging to NAD-dependent epimerase/dehydratase family, and its co-factor binding sites and substrate binding sites were highly conserved. [Conclusion] DNA and cDNA sequences of CCR gene were obtained from P. purpureum, which had the typical characteristics of other homologous genes. The obtained bioinformatic data provided theoretical references for the further analysis of CCR and better application of P. purpureum in the future.
