Litchi anthracnose caused by Colletotrictum gloeosporioides （Penz） Saec. is an extremely destructive and widely distributed disease, which results in poor market value. Borate, an essential plant micronutrient that ...Litchi anthracnose caused by Colletotrictum gloeosporioides （Penz） Saec. is an extremely destructive and widely distributed disease, which results in poor market value. Borate, an essential plant micronutrient that helps plant growth and has been used extensively in industry and agriculture as a safe method for control of fungi, was effective in the form of potassium tetraborate for control of C. gloeosporioides （Penz）. In this study, boron strongly inhibited spore germina- tion, germ tube elongation, and mycelial spread of C. gloeosporioides （Penz） in the culture medium. Application of boron at 1% caused the appearance of abnor- mal spores （disrupted） in some cases. On the basis of propidium iodide fluorescent staining, the loss of membrane integrity in C. gloeosporioides （Penz） was ob- served after boron treatment. Furthermore, Boron led to the leakage of cellular constituents （soluble proteins and carbohydrates） from hyphae of C. gloeosporioides （Penz）. These data suggest that the mechanisms may be directly related with the disruption effect of boron on cell membrane of the fungal pathogen, resulting in the breakdown of cell membrane structure and loss of cytoplasmic materials from the hyphae.展开更多
[Objective]The pathogen of pineapple anthracnose were isolated and identified,and the biological characteristics of the pathogen were studied.[Method]Pathogen was isolated and purified from the leaves collected from d...[Objective]The pathogen of pineapple anthracnose were isolated and identified,and the biological characteristics of the pathogen were studied.[Method]Pathogen was isolated and purified from the leaves collected from different areas in Hainan province which had been infected by pineapple anthracnose,and the biological characteristics were studied as well as morphological identification of the pathogen.[Results] The pathogen of pineapple anthracnose was identified as Colletorichum gloeosprioides Penz.through morphological characteristics.The results of biological characteristic test showed that the optimum temperature for mycelium growth and spore germination were 25-30 and 28-30 ℃,respectively;and the optimum pH value was 6.0-8.0;complete darkness was the best condition for growth of mycelium,while alternative light and darkness was most beneficial for spore germination;the best carbon sources for mycelium growth were glucose,D-fructose and maltose;except for L-arabinose and D-galactose,the other carbon sources all had promotion effect on spore germination;yeast extract,peptone and beef extract were proved to be beneficial for mycelium growth and spore germination.Aspartic acid was only beneficial for spore germination;spores were just able to germinate when the relative humidity（RH）was greater than 90%,spore germination was the highest under water drops condition.[Conclusion]The results laid preliminary basis for the control of pineapple anthracnose.展开更多
基金Supported by China Agriculture Research System(CARS-33-25)
文摘Litchi anthracnose caused by Colletotrictum gloeosporioides （Penz） Saec. is an extremely destructive and widely distributed disease, which results in poor market value. Borate, an essential plant micronutrient that helps plant growth and has been used extensively in industry and agriculture as a safe method for control of fungi, was effective in the form of potassium tetraborate for control of C. gloeosporioides （Penz）. In this study, boron strongly inhibited spore germina- tion, germ tube elongation, and mycelial spread of C. gloeosporioides （Penz） in the culture medium. Application of boron at 1% caused the appearance of abnor- mal spores （disrupted） in some cases. On the basis of propidium iodide fluorescent staining, the loss of membrane integrity in C. gloeosporioides （Penz） was ob- served after boron treatment. Furthermore, Boron led to the leakage of cellular constituents （soluble proteins and carbohydrates） from hyphae of C. gloeosporioides （Penz）. These data suggest that the mechanisms may be directly related with the disruption effect of boron on cell membrane of the fungal pathogen, resulting in the breakdown of cell membrane structure and loss of cytoplasmic materials from the hyphae.
基金Supported by Basic Research Operational Funds in Central Nonprofit Research Institutes"Research on Control Technology of the Major Pests and Diseases in Pineapple"(NO.2009hzs1J027)Scientific Operating Expenses in Hainan Province"Research on Demonstration of Quality Variety Introduction and Rapid Propagation Technology of Pineapple"(Qiong Financial Budget[2007]NO.134)Scientific Operating Expenses in Hainan Province"Research on Safety Regulation during TN 16 Pineapple Production Stage and Enhancement of Fruit Quality Technology"(Qiong Financial Budget[2010]NO.139)~~
文摘[Objective]The pathogen of pineapple anthracnose were isolated and identified,and the biological characteristics of the pathogen were studied.[Method]Pathogen was isolated and purified from the leaves collected from different areas in Hainan province which had been infected by pineapple anthracnose,and the biological characteristics were studied as well as morphological identification of the pathogen.[Results] The pathogen of pineapple anthracnose was identified as Colletorichum gloeosprioides Penz.through morphological characteristics.The results of biological characteristic test showed that the optimum temperature for mycelium growth and spore germination were 25-30 and 28-30 ℃,respectively;and the optimum pH value was 6.0-8.0;complete darkness was the best condition for growth of mycelium,while alternative light and darkness was most beneficial for spore germination;the best carbon sources for mycelium growth were glucose,D-fructose and maltose;except for L-arabinose and D-galactose,the other carbon sources all had promotion effect on spore germination;yeast extract,peptone and beef extract were proved to be beneficial for mycelium growth and spore germination.Aspartic acid was only beneficial for spore germination;spores were just able to germinate when the relative humidity（RH）was greater than 90%,spore germination was the highest under water drops condition.[Conclusion]The results laid preliminary basis for the control of pineapple anthracnose.
