Recently,in-situ polymerization inside living cells has attracted much attention due to the efficient cellular internalization and elevated drug retention.However,the lack of tracking of the in-situ polymerization pro...Recently,in-situ polymerization inside living cells has attracted much attention due to the efficient cellular internalization and elevated drug retention.However,the lack of tracking of the in-situ polymerization process and the unclear effects of polymerization on cellular functions restrict its biomedical applications.Herein,we designed a Y-shaped diacetylene-containing lipidated peptide amphiphile(YDLPA1)with positive charges,which underwent in-situ polymerization initiated by reactive oxygen species in the intracellular microenvironment.In comparison,zwitterionic YDLPA2 and negatively charged Y-DLPA3 were polymerized in aqueous solution,but cannot polymerize in the intracellular microenvironment.The polymerized Y-DLPA1 with red fluorescence provides a platform to label cells for long-term tracking studies.This polymerization reaction induced tumor cell apoptosis,increased cell viscosity and decreased cell motility,which potentially inhibited tumor metastasis and served as a novel antitumor agent.This work provides a novel strategy to track in-situ polymerization process and modulate cell biofunctions.展开更多
基金supported by the National Natural Science Foundation of China(52173124)the Fundamental Research Funds for the Central Universities(2172019kfyXJJS070)。
文摘Recently,in-situ polymerization inside living cells has attracted much attention due to the efficient cellular internalization and elevated drug retention.However,the lack of tracking of the in-situ polymerization process and the unclear effects of polymerization on cellular functions restrict its biomedical applications.Herein,we designed a Y-shaped diacetylene-containing lipidated peptide amphiphile(YDLPA1)with positive charges,which underwent in-situ polymerization initiated by reactive oxygen species in the intracellular microenvironment.In comparison,zwitterionic YDLPA2 and negatively charged Y-DLPA3 were polymerized in aqueous solution,but cannot polymerize in the intracellular microenvironment.The polymerized Y-DLPA1 with red fluorescence provides a platform to label cells for long-term tracking studies.This polymerization reaction induced tumor cell apoptosis,increased cell viscosity and decreased cell motility,which potentially inhibited tumor metastasis and served as a novel antitumor agent.This work provides a novel strategy to track in-situ polymerization process and modulate cell biofunctions.
