[目的]通过制作脊髓型减压病大鼠模型，观察成年大鼠脊髓受损后不同时期血管内皮生长因子（VEGF）含量的变化。[方法]健康雄性SD大鼠24只，鼠龄120～250 d ，体质量200～250 g ，24 h昼夜循环光照，自由饮水、取食，室温（24±2）...[目的]通过制作脊髓型减压病大鼠模型，观察成年大鼠脊髓受损后不同时期血管内皮生长因子（VEGF）含量的变化。[方法]健康雄性SD大鼠24只，鼠龄120～250 d ，体质量200～250 g ，24 h昼夜循环光照，自由饮水、取食，室温（24±2）℃。所有动物术前均经 Y 型电迷宫测试，然后随机分为三组，每组各8只。第一组：正常对照组；第二组：安全减压组；第三组：减压病组。各组分别于出舱后1d、1周、2周、4周和8周进行血清VEGF含量检测。[结果]正常对照组，安全减压组显示出舱后1 d血清VEGF含量较少，减压病组血清VEGF含量较多，与正常对照组，安全减压组相比差异有显著性（ P ＜0．05），减压病组血清V EG F含量1周后下调，1周、2周减压病组与正常对照组，安全减压组相比差异亦有显著性（ P ＜0．05），4周、8周时各组比较差异均无显著性（ P ＞0．05）。[结论]脊髓型减压病脊髓损伤可上调血清VEGF含量，从而对脊髓损伤起到指导治疗作用。展开更多
Objective： To investigate the effect of electroacupuncture （EA） at Ganshu （BL 18） and Shenshu （BL 23） on vascular endothelial growth factor （VEGF） and platelet endothelial cell adhesion molecule-1 （PECAM-1）...Objective： To investigate the effect of electroacupuncture （EA） at Ganshu （BL 18） and Shenshu （BL 23） on vascular endothelial growth factor （VEGF） and platelet endothelial cell adhesion molecule-1 （PECAM-1）/CD31 around the cerebral infarction focus in middle cerebral artery occlusion （MCAO） rats and the possible mechanism, thus to provide a new strategy for the treatment of cerebral ischemic stroke by acupuncture. Methods： A total of 180 healthy male Sprague-Dawley （SD） rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, 45 rats in each group. MCAO model was established using the modified line-embolus method in all rats except for those in the sham operation group; rats in the acupoint group were treated with EA at Ganshu （BL 18） and Shenshu （BL 23）; rats in the non-acupoint group were treated with EA at the control points; rats in other 2 groups were only subjected to bundling without treatment. Ten rats in each group were randomly selected on the 3rd day, the 14th day and the 21st day after acupuncture stimulation to test the neurological function impairment. The expression levels of CD31 and VEGF were also detected. Results： Compared with the model group and non-acupoint group, the neurological function score of the acupoint group was decreased at each time point, and the differences were statistically significant （P〈0.05, P〈0.01）. The expressions of VEGF and CD31 in each group were the lowest on the 3rd day, reached the peak on the 14th day and still remained at high level on the 21st day. And the differences among groups were statistically significant both on the 14th day and the 21st day （P〈0.05, P〈0.01）. Compared with the model group and the non-acupoint group, the expressions of VEGF and CD31 in the acupoint group were increased, and the differences were statistically significant （all P〈0.05）. Conclusion： EA at Ganshu （BL 18） and Shenshu （BL 23） can significantly improve the neurological function score of MCAO model rats, and shows protective effect on cerebral ischemia. The protective mechanism may be related to the up-regulation of CD31 and VEGF expression around the cerebral infarction focus in the MCAO model rats and induction of angiogenesis.展开更多
文摘[目的]通过制作脊髓型减压病大鼠模型，观察成年大鼠脊髓受损后不同时期血管内皮生长因子（VEGF）含量的变化。[方法]健康雄性SD大鼠24只，鼠龄120～250 d ，体质量200～250 g ，24 h昼夜循环光照，自由饮水、取食，室温（24±2）℃。所有动物术前均经 Y 型电迷宫测试，然后随机分为三组，每组各8只。第一组：正常对照组；第二组：安全减压组；第三组：减压病组。各组分别于出舱后1d、1周、2周、4周和8周进行血清VEGF含量检测。[结果]正常对照组，安全减压组显示出舱后1 d血清VEGF含量较少，减压病组血清VEGF含量较多，与正常对照组，安全减压组相比差异有显著性（ P ＜0．05），减压病组血清V EG F含量1周后下调，1周、2周减压病组与正常对照组，安全减压组相比差异亦有显著性（ P ＜0．05），4周、8周时各组比较差异均无显著性（ P ＞0．05）。[结论]脊髓型减压病脊髓损伤可上调血清VEGF含量，从而对脊髓损伤起到指导治疗作用。
基金supported by Open Fund for Colleges and Universities Innovation Platform of Hunan Province, No.14K070Key Project of Hunan Province Administration of Traditional Chinese Medicine, No. 201310~~
文摘Objective： To investigate the effect of electroacupuncture （EA） at Ganshu （BL 18） and Shenshu （BL 23） on vascular endothelial growth factor （VEGF） and platelet endothelial cell adhesion molecule-1 （PECAM-1）/CD31 around the cerebral infarction focus in middle cerebral artery occlusion （MCAO） rats and the possible mechanism, thus to provide a new strategy for the treatment of cerebral ischemic stroke by acupuncture. Methods： A total of 180 healthy male Sprague-Dawley （SD） rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, 45 rats in each group. MCAO model was established using the modified line-embolus method in all rats except for those in the sham operation group; rats in the acupoint group were treated with EA at Ganshu （BL 18） and Shenshu （BL 23）; rats in the non-acupoint group were treated with EA at the control points; rats in other 2 groups were only subjected to bundling without treatment. Ten rats in each group were randomly selected on the 3rd day, the 14th day and the 21st day after acupuncture stimulation to test the neurological function impairment. The expression levels of CD31 and VEGF were also detected. Results： Compared with the model group and non-acupoint group, the neurological function score of the acupoint group was decreased at each time point, and the differences were statistically significant （P〈0.05, P〈0.01）. The expressions of VEGF and CD31 in each group were the lowest on the 3rd day, reached the peak on the 14th day and still remained at high level on the 21st day. And the differences among groups were statistically significant both on the 14th day and the 21st day （P〈0.05, P〈0.01）. Compared with the model group and the non-acupoint group, the expressions of VEGF and CD31 in the acupoint group were increased, and the differences were statistically significant （all P〈0.05）. Conclusion： EA at Ganshu （BL 18） and Shenshu （BL 23） can significantly improve the neurological function score of MCAO model rats, and shows protective effect on cerebral ischemia. The protective mechanism may be related to the up-regulation of CD31 and VEGF expression around the cerebral infarction focus in the MCAO model rats and induction of angiogenesis.
