On basis of thermodynamic empirical equations, the thermodynamic parameters for the direct amination of isobutylene to tert‐butylamine, an atomically economic and green chemical reaction,were calculated. In particula...On basis of thermodynamic empirical equations, the thermodynamic parameters for the direct amination of isobutylene to tert‐butylamine, an atomically economic and green chemical reaction,were calculated. In particular, the equilibrium conversion of isobutylene under various reactionconditions close to those used in industry was calculated and discussed. Isobutylene amination is atemperature sensitive reaction due to its exothermic nature and isobutylene equilibrium conversiondecreases with temperature. However, kinetically, the amination reaction will be faster at ahigher temperature. Thus, there must be an optimum temperature for the reaction. A high pressureand n(NH3)/n(i‐C4H8) molar ratio promote the transformation of isobutylene to tert‐butylamine.Developing a highly efficient catalyst under mild reaction conditions is preferred for the aminationprocess. The reaction was investigated over a series of acidic zeolites. ZSM‐11 zeolite exhibited thebest performance with 14.2% isobutylene conversion (52.2% of the equilibrium conversion) and >99.0% tert‐butylamine selectivity. The effect of reaction conditions on the performance of the ZSM‐11 catalyst agreed with the thermodynamic results, which provides guidance for further catalyst development and reaction condition optimization.展开更多
Objective:To evaluate the efficacy of combination perindopril/amlodipine tablets in patients with high-altitude hypertension who were previously unable to control their blood pressure with monotherapy.Methods:A total ...Objective:To evaluate the efficacy of combination perindopril/amlodipine tablets in patients with high-altitude hypertension who were previously unable to control their blood pressure with monotherapy.Methods:A total of 151 patients with high-altitude hypertension whose blood pressure remained inadequately controlled with previous monotherapy were enrolled in this study.All patients received an 8-week treatment with a combination of perindopril/amlodipine tablets,consisting of perindopril 10 mg/day and amlodipine 5 mg/day.Blood pressure measurements,including both diastolic and systolic pressures,were taken at baseline,and after 2,4,6,and 8 weeks of treatment.Results:After 8 weeks of treatment,there was a significant reduction in both average systolic and diastolic blood pressure compared to baseline(P<0.0001).Specifically,the average systolic blood pressure decreased by 24.45±13.75 mmHg,and the average diastolic blood pressure decreased by 13.37±8.40 mmHg.The overall heart rate showed no significant changes during the treatment period.Conclusion:A combination of perindopril/amlodipine tablets significantly improved blood pressure control in patients with high-altitude hypertension after 8 weeks of treatment.These results support the efficacy of combination perindopril/amlodipine as a viable treatment option for high-altitude hypertension.展开更多
目的探究左西孟旦联合培哚普利叔丁胺治疗充血性心力衰竭(CHF)的效果。方法选取邢台市中心医院收治的126例CHF患者为研究对象,使用抽签法将其随机分为对照组和联合组,每组63例。两组均给予基础治疗,对照组在基础治疗上给予培哚普利叔丁...目的探究左西孟旦联合培哚普利叔丁胺治疗充血性心力衰竭(CHF)的效果。方法选取邢台市中心医院收治的126例CHF患者为研究对象,使用抽签法将其随机分为对照组和联合组,每组63例。两组均给予基础治疗,对照组在基础治疗上给予培哚普利叔丁胺治疗,联合组在对照组基础上给予左西孟旦治疗。比较两组治疗前后心功能指标、血清NT-proBNP、CK-MB、Cys-C水平、炎症因子水平,不良反应及临床疗效。结果联合组临床疗效总有效率高于对照组(P<0.05);治疗后,两组左心室射血分数(LVEF)、每搏量(SV)水平、6 min步行距离(WT)均增加,且联合组LLVEF、SV水平、6 min WT均高于对照组(P<0.05);治疗后,两组NT-proBNP、CK-MB及Cys-C水平均降低,且联合组NT-proBNP、CK-MB及Cys-C水平低于对照组(P<0.05);治疗后,两组肿瘤坏死因子-α(TNF-α)、超敏C反应蛋白(hs-CRP)和白细胞介素-6(IL-6)水平均降低,且联合组TNF-α、hs-CRP及IL-6水平低于对照组(P<0.05);两组不良反应差异无统计学意义(P>0.05)。结论与培哚普利叔丁胺治疗CHF相比,培哚普利叔丁胺联合左西孟旦在临床疗效、心功能、心肌损伤和炎症反应方面改善效果显著,且安全可靠。展开更多
Simple and sensitive methods were developed for the determination of indapamide, perindopril and its active metabolite perindoprilat in human plasma or whole blood by hyphenated ultra-performance li- quid chromatograp...Simple and sensitive methods were developed for the determination of indapamide, perindopril and its active metabolite perindoprilat in human plasma or whole blood by hyphenated ultra-performance li- quid chromatography-mass spectrometry （UPLC-MS/MS）. lndapamide-d3, perindopril-d4 and perindoprilat-d4 were used as the internal standards. The separation was performed on a Thermo BDS Hypersil C18 column （4.6 mm × 100 mm, 2.4 μm） for indapamide and perindopril simultaneously following a protein precipitation pretreatment of the biosamples. The separation of perindoprilat was achieved independently on a phenomenex PFP column （4.6 mm × 150 mm, 5 μm）. All the analytes were quantitated with positive electrospray ionization and multiple reactions monitoring mode. The assay exhibited a linear range of 1-250 ng/mL for indapamide, 0.4-100 ng/mL for perindopril and 0.2-20 ng/mL for perindoprilat. The methods were fully validated to meet the requirements for bioassay in accuracy, precision, recovery, reproducibility, stabilities and matrix effects, and successfully applied to the pharmacokinetic study of perindopril tert-butylamine/indapamide compound tablets in Chinese healthy volunteers and the comparative pharmacokinetic study between plasma and whole blood.展开更多
Objective: This study was performed to evaluate whether implantation of mesenchymal stem cell (MSC) would reduce left ventricular remodelling from the molecular mechanisms compared with angiotensin-converting enzyme i...Objective: This study was performed to evaluate whether implantation of mesenchymal stem cell (MSC) would reduce left ventricular remodelling from the molecular mechanisms compared with angiotensin-converting enzyme inhibitors (ACEIs) perindopril into ischemic myocardium after acute myocardial infarction. Methods: Forty rats were divided into four groups: control, MSC, ACEI, MSC+ACEI groups. Bone marrow stem cell derived rat was injected immediately into a zone made ischemic by coronary artery ligation in MSC group and MSC+ACEI group. Phosphate-buffered saline (PBS) was injected into control group. Perindopril was administered p.o. to ACEI group and MSC+ACEI group. Six weeks after implantation, the rats were killed and heart sample was collected. Fibrillar collagen was observed by meliorative Masson’s trichome stain. Western Blotting was employed to evaluate the protein expression of matrix metalloproteinase (MMP)-2, matrix metalloproteinase (MMP)-9 in infarction zone. The transcriptional level of MMP2, MMP9 and tissue inhibitor of matrix metalloproteinase (TIMP)-1 in infarction area was detected by reverse transcriptase PCR (RT-PCR) analysis. Results: The fibrillar collagen area, the protein expression of MMP2, MMP9 and the transcriptional level of MMP2, MMP9 mRNA in infarction zone reduced in MSC group, ACEI group, and MSC+ACEI group. No significant difference was detected in the expression of TIMP1 mRNA among the 4 groups. Conclusion: Both MSC and ACEI could reduce infarction remodelling by altering collagen metabolism.展开更多
基金supported by K. C. Wong Education FoundationYouth Innovation Promotion Association of CAS (20120155)Dalian Eminent Young Scientist Program (2015R009)~~
文摘On basis of thermodynamic empirical equations, the thermodynamic parameters for the direct amination of isobutylene to tert‐butylamine, an atomically economic and green chemical reaction,were calculated. In particular, the equilibrium conversion of isobutylene under various reactionconditions close to those used in industry was calculated and discussed. Isobutylene amination is atemperature sensitive reaction due to its exothermic nature and isobutylene equilibrium conversiondecreases with temperature. However, kinetically, the amination reaction will be faster at ahigher temperature. Thus, there must be an optimum temperature for the reaction. A high pressureand n(NH3)/n(i‐C4H8) molar ratio promote the transformation of isobutylene to tert‐butylamine.Developing a highly efficient catalyst under mild reaction conditions is preferred for the aminationprocess. The reaction was investigated over a series of acidic zeolites. ZSM‐11 zeolite exhibited thebest performance with 14.2% isobutylene conversion (52.2% of the equilibrium conversion) and >99.0% tert‐butylamine selectivity. The effect of reaction conditions on the performance of the ZSM‐11 catalyst agreed with the thermodynamic results, which provides guidance for further catalyst development and reaction condition optimization.
文摘Objective:To evaluate the efficacy of combination perindopril/amlodipine tablets in patients with high-altitude hypertension who were previously unable to control their blood pressure with monotherapy.Methods:A total of 151 patients with high-altitude hypertension whose blood pressure remained inadequately controlled with previous monotherapy were enrolled in this study.All patients received an 8-week treatment with a combination of perindopril/amlodipine tablets,consisting of perindopril 10 mg/day and amlodipine 5 mg/day.Blood pressure measurements,including both diastolic and systolic pressures,were taken at baseline,and after 2,4,6,and 8 weeks of treatment.Results:After 8 weeks of treatment,there was a significant reduction in both average systolic and diastolic blood pressure compared to baseline(P<0.0001).Specifically,the average systolic blood pressure decreased by 24.45±13.75 mmHg,and the average diastolic blood pressure decreased by 13.37±8.40 mmHg.The overall heart rate showed no significant changes during the treatment period.Conclusion:A combination of perindopril/amlodipine tablets significantly improved blood pressure control in patients with high-altitude hypertension after 8 weeks of treatment.These results support the efficacy of combination perindopril/amlodipine as a viable treatment option for high-altitude hypertension.
文摘目的探究左西孟旦联合培哚普利叔丁胺治疗充血性心力衰竭(CHF)的效果。方法选取邢台市中心医院收治的126例CHF患者为研究对象,使用抽签法将其随机分为对照组和联合组,每组63例。两组均给予基础治疗,对照组在基础治疗上给予培哚普利叔丁胺治疗,联合组在对照组基础上给予左西孟旦治疗。比较两组治疗前后心功能指标、血清NT-proBNP、CK-MB、Cys-C水平、炎症因子水平,不良反应及临床疗效。结果联合组临床疗效总有效率高于对照组(P<0.05);治疗后,两组左心室射血分数(LVEF)、每搏量(SV)水平、6 min步行距离(WT)均增加,且联合组LLVEF、SV水平、6 min WT均高于对照组(P<0.05);治疗后,两组NT-proBNP、CK-MB及Cys-C水平均降低,且联合组NT-proBNP、CK-MB及Cys-C水平低于对照组(P<0.05);治疗后,两组肿瘤坏死因子-α(TNF-α)、超敏C反应蛋白(hs-CRP)和白细胞介素-6(IL-6)水平均降低,且联合组TNF-α、hs-CRP及IL-6水平低于对照组(P<0.05);两组不良反应差异无统计学意义(P>0.05)。结论与培哚普利叔丁胺治疗CHF相比,培哚普利叔丁胺联合左西孟旦在临床疗效、心功能、心肌损伤和炎症反应方面改善效果显著,且安全可靠。
基金supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions and the Policy Directive Program of Jiangsu Province(BY2015072-03)
文摘Simple and sensitive methods were developed for the determination of indapamide, perindopril and its active metabolite perindoprilat in human plasma or whole blood by hyphenated ultra-performance li- quid chromatography-mass spectrometry （UPLC-MS/MS）. lndapamide-d3, perindopril-d4 and perindoprilat-d4 were used as the internal standards. The separation was performed on a Thermo BDS Hypersil C18 column （4.6 mm × 100 mm, 2.4 μm） for indapamide and perindopril simultaneously following a protein precipitation pretreatment of the biosamples. The separation of perindoprilat was achieved independently on a phenomenex PFP column （4.6 mm × 150 mm, 5 μm）. All the analytes were quantitated with positive electrospray ionization and multiple reactions monitoring mode. The assay exhibited a linear range of 1-250 ng/mL for indapamide, 0.4-100 ng/mL for perindopril and 0.2-20 ng/mL for perindoprilat. The methods were fully validated to meet the requirements for bioassay in accuracy, precision, recovery, reproducibility, stabilities and matrix effects, and successfully applied to the pharmacokinetic study of perindopril tert-butylamine/indapamide compound tablets in Chinese healthy volunteers and the comparative pharmacokinetic study between plasma and whole blood.
文摘Objective: This study was performed to evaluate whether implantation of mesenchymal stem cell (MSC) would reduce left ventricular remodelling from the molecular mechanisms compared with angiotensin-converting enzyme inhibitors (ACEIs) perindopril into ischemic myocardium after acute myocardial infarction. Methods: Forty rats were divided into four groups: control, MSC, ACEI, MSC+ACEI groups. Bone marrow stem cell derived rat was injected immediately into a zone made ischemic by coronary artery ligation in MSC group and MSC+ACEI group. Phosphate-buffered saline (PBS) was injected into control group. Perindopril was administered p.o. to ACEI group and MSC+ACEI group. Six weeks after implantation, the rats were killed and heart sample was collected. Fibrillar collagen was observed by meliorative Masson’s trichome stain. Western Blotting was employed to evaluate the protein expression of matrix metalloproteinase (MMP)-2, matrix metalloproteinase (MMP)-9 in infarction zone. The transcriptional level of MMP2, MMP9 and tissue inhibitor of matrix metalloproteinase (TIMP)-1 in infarction area was detected by reverse transcriptase PCR (RT-PCR) analysis. Results: The fibrillar collagen area, the protein expression of MMP2, MMP9 and the transcriptional level of MMP2, MMP9 mRNA in infarction zone reduced in MSC group, ACEI group, and MSC+ACEI group. No significant difference was detected in the expression of TIMP1 mRNA among the 4 groups. Conclusion: Both MSC and ACEI could reduce infarction remodelling by altering collagen metabolism.
