Circadian clock genes are crucial for generating and sustaining most rhythmic daily functions in the animal kingdom,which entrain the rhythms of biochemical,physiological,and behavioural processes.To better understand...Circadian clock genes are crucial for generating and sustaining most rhythmic daily functions in the animal kingdom,which entrain the rhythms of biochemical,physiological,and behavioural processes.To better understand the molecular oscillations of the circadian rhythms in darkbarbel catfish(Pelteobagrus vachellii),we isolated and characterized two circadian clock genes in P.vachellii,period 1(per1),and period 3(per3).The circadian clock gene per1 was found to encode a 1428-amino acid polypeptide,including PER-ARNT-SIM(PAS)dimerisation domains,a PAS-associated C-terminal motif(PAC),a short mutable domain(S/M),and a nuclear export signal(NES).The 4902-bp per3 cDNA includes an open reading frame encoding a 1292-amino acid residue polypeptide with a PER-ARNT-SIM(PAS)domain,cytoplasmic localisation domain(CLD),interaction site(TIS),and a nuclear localisation signal(NLS).The per1 and per3 gene was constitutively expressed in all examined tissues.Moreover,per1 expression within a light/dark cycles showed rhythmic expression in the diencephalon,brain,liver and intestine,with the acrophase at 15:15,12:52,7:51 and 12:55,respectively.Daily expression of per3 was rhythmic in the diencephalonbrain,liver and intestine,with the acrophase at 8:15,9:54,10:39,and 10:25 h,respectively.These findings expand our understanding of circadian mechanism at the molecular level in this species.展开更多
探讨中国人群生物钟Period1(Per1)基因rs2585405、rs2253820和rs2735611位点单核苷酸多态性(single-nucleotide?polymorphisms,SNP)与原发性失眠(primary insomnia,PI)的相关性。方法:采用病例对照研究,选取157例PI者为实验组,并匹配13...探讨中国人群生物钟Period1(Per1)基因rs2585405、rs2253820和rs2735611位点单核苷酸多态性(single-nucleotide?polymorphisms,SNP)与原发性失眠(primary insomnia,PI)的相关性。方法:采用病例对照研究,选取157例PI者为实验组,并匹配133名健康者为对照组。采用MassARRAY SNP 基因分型技术检测Per1基因rs2585405、rs2253820和rs2735611位点多态性。结果:①Per1基因rs2585405、rs2253820和rs2735611位点多态性在PI和对照组的基因型分布均符合Hardy-Weinberg平衡(P>0.05)。②PI组和对照组Per1基因3个SNP位点的基因型和等位基因频率比较,均没有显著性差异(P>0.05)。③PI组和对照组Per1基因3个SNP位点进行单倍型分析,显示构建的3种单倍型之间均没有显著差异(p>0.05)。结论:我们选择标记的Per1基因3个SNP位点可能不是中国人群PI的易感位点。展开更多
基金Supported by the National Natural Science Foundation of China(No.31402305)the Sichuan Science and Technology Program(Nos.2017JY0161,2018NZ0119)。
文摘Circadian clock genes are crucial for generating and sustaining most rhythmic daily functions in the animal kingdom,which entrain the rhythms of biochemical,physiological,and behavioural processes.To better understand the molecular oscillations of the circadian rhythms in darkbarbel catfish(Pelteobagrus vachellii),we isolated and characterized two circadian clock genes in P.vachellii,period 1(per1),and period 3(per3).The circadian clock gene per1 was found to encode a 1428-amino acid polypeptide,including PER-ARNT-SIM(PAS)dimerisation domains,a PAS-associated C-terminal motif(PAC),a short mutable domain(S/M),and a nuclear export signal(NES).The 4902-bp per3 cDNA includes an open reading frame encoding a 1292-amino acid residue polypeptide with a PER-ARNT-SIM(PAS)domain,cytoplasmic localisation domain(CLD),interaction site(TIS),and a nuclear localisation signal(NLS).The per1 and per3 gene was constitutively expressed in all examined tissues.Moreover,per1 expression within a light/dark cycles showed rhythmic expression in the diencephalon,brain,liver and intestine,with the acrophase at 15:15,12:52,7:51 and 12:55,respectively.Daily expression of per3 was rhythmic in the diencephalonbrain,liver and intestine,with the acrophase at 8:15,9:54,10:39,and 10:25 h,respectively.These findings expand our understanding of circadian mechanism at the molecular level in this species.
文摘探讨中国人群生物钟Period1(Per1)基因rs2585405、rs2253820和rs2735611位点单核苷酸多态性(single-nucleotide?polymorphisms,SNP)与原发性失眠(primary insomnia,PI)的相关性。方法:采用病例对照研究,选取157例PI者为实验组,并匹配133名健康者为对照组。采用MassARRAY SNP 基因分型技术检测Per1基因rs2585405、rs2253820和rs2735611位点多态性。结果:①Per1基因rs2585405、rs2253820和rs2735611位点多态性在PI和对照组的基因型分布均符合Hardy-Weinberg平衡(P>0.05)。②PI组和对照组Per1基因3个SNP位点的基因型和等位基因频率比较,均没有显著性差异(P>0.05)。③PI组和对照组Per1基因3个SNP位点进行单倍型分析,显示构建的3种单倍型之间均没有显著差异(p>0.05)。结论:我们选择标记的Per1基因3个SNP位点可能不是中国人群PI的易感位点。
