Periplaneta Americana Extract Ameliorates LPS-induced Acute Lung Injury Via Reducing Inflammation and Oxidative Stress

Objective Acute lung injury(ALI)is an acute clinical syndrome characterized by uncontrolled inflammation response,which causes high mortality and poor prognosis.The present study determined the protective effect and underlying mechanism of Periplaneta americana extract(PAE)against lipopolysaccharide(LPS)-induced ALI.Methods The viability of MH-S cells was measured by MTT.ALI was induced in BALB/c mice by intranasal administration of LPS(5 mg/kg),and the pathological changes,oxidative stress,myeloperoxidase activity,lactate dehydrogenase activity,inflammatory cytokine expression,edema formation,and signal pathway activation in lung tissues and bronchoalveolar lavage fluid(BALF)were examined by H&E staining,MDA,SOD and CAT assays,MPO assay,ELISA,wet/dry analysis,immunofluorescence staining and Western blotting,respectively.Results The results revealed that PAE obviously inhibited the release of proinflammatory TNF-α,IL-6 and IL-1βby suppressing the activation of MAPK/Akt/NF-κB signaling pathways in LPS-treated MH-S cells.Furthermore,PAE suppressed the neutrophil infiltration,permeability increase,pathological changes,cellular damage and death,pro-inflammatory cytokines expression,and oxidative stress upregulation,which was associated with its blockage of the MAPK/Akt/NF-κB pathway in lung tissues of ALI mice.Conclusion PAE may serve as a potential agent for ALI treatment due to its anti-inflammatory and anti-oxidative properties,which correlate to the blockage of the MAPK/NF-κB and AKT signaling pathways.

Potential Risk of Transmission of Escherichia coli and Salmonella spp. Infections by the Musca domestica Fly and the Periplaneta americana Cockroach in the City of Cotonou (South Benin)

Background: The occurrence of bacterial infections sometimes involves synanthropic flies and cockroaches, as mechanical vectors of pathogenic microorganisms. The aim of this study was to identify the different species of flies and cockroaches that cohabit with humans in the city of Cotonou and determine the contribution of Musca domestica and Periplaneta americana in the transmission of bacteria responsible for infections in humans. Methods: Capture sessions during the day for flies and at night for cockroaches were carried out in 4 arrondissements of the city of Cotonou. The insects collected were transported to the Center of Research Entomological of Cotonou for identification, then sent to the Section Hygiene of Water and Food for microbiological analysis, enumeration and testing for human pathogenic bacteria. Results: A total of 351 flies and 26 cockroaches were collected at the sites, including two synanthropic species belonging to two families each. Musca domestica and Periplaneta americana were the most abundant species found after identification in the city of Cotonou. Thermo-tolerant coliforms and Escherichia coli were found on all specimens at varying concentrations. Salmonella spp. bacteria were identified in the fly population from the Dantokpa dump. Conclusion: Flies and cockroaches can transmit microorganisms to humans. The presence of Salmonella spp. among the specimens revealed that infections frequently caused by contaminated food or water are also transmitted to humans by flies. The presence of these germs on flies and cockroaches represents a potential risk of mechanical transmission to humans. It is, therefore, essential to continue investigations in order to assess the species of bacteria propagated, improve control strategies against these troublesome insects and adopt better hygiene conditions for better living.

Research Advances in Pharmacological Action and Clinical Application of Periplaneta americana

Researches on pharmacological action and drug development of Periplane- ta americana have demonstrated that it has wide medicinal value, low side effects and rich resources, having good application prospects in medicine field. This article reviewed the researches on pharmacological action and clinical application of Periplaneta americana in order to provide references for the further research and devel- opment of Periplaneta americana medicines.

美洲大蠊(Periplaneta americana L.)抗菌肽杀菌作用初步观察

报道了美洲大蠊(PeriplanetaamericanaL.)抗菌肽对大肠杆菌(EscherichiacoliK88)的作用过程.电镜观察表明,美洲大蠊抗菌肽首先使细菌的外层及细胞质膜损伤,形成开口,导致内容物外泄而死亡,最后菌体崩解成碎片.

Periplaneta americana extract used in patients with systemic inflammatory response syndrome

BACKGROUND:Periplaneta americana extract is recognized to have a positive effect on gastrointestinal mucosa.This study aimed to investigate the effects of periplaneta americana extract on immune function,nutrition status and gastrointestinal complications of early enteral nutrition patients with systemic inflammatory response syndrome(SIRS).METHODS:Patients with SIRS were randomly divided into two groups:treatment and control groups.All patients in the two groups received conventional therapy including enteral nutrition,but periplaneta americana extract,an additional Chinese medicine,was given to the patients in the treatment group.At the beginning of treatment(0 day)and 1,3,and 7 days after treatment,the levels of immunoglobulin(Ig A),total lymphocyte count(TLC),total protein(TP)and prealbumin(PA)were respectively tested in patients'venous blood.The incidences of bloating,diarrhea,aspiration pneumonia and high blood sugar at 7 days after treatment were recorded.The mortality of the patients in 28 days was recorded.RESULTS:At 3 and 7 days after treatment,the levels of Ig A and TLC in the treatment group were higher than those in the control group(P<0.05).At 7 days after treatment,the levels of TP and PA in the treatment group were higher than those in the control group(P<0.05).The incidences of bloating and diarrhea in the treatment group were lower than those in the control group,the differences were significant(P<0.05).The mortality of treatment group was lower than that of the control group(P>0.05).CONCLUSION:Periplaneta americana extract could reduce gastrointestinal complications and improve immune function and nutritional status in patients with systemic inflammatory response syndrome.

Comparison between Decolorization Effects of Activated Carbon and Macroporous Resin on Periplaneta americana L. Skimmed Cream

[Objective] The aim of this study was to investigate the decolorization ef- fects of activated carbon and macroporous resin on Periplaneta americana L. skimmed cream and compare the advantages and disadvantages of the two decolorization technologies. [Method] Periplaneta americana L. skimmed cream was decolored with activated carbon and macroporous resin, and freeze-dried to collect solid decolorization products. By investigating the yield, decolorization rate, protein retention rate and decolorization operation process, the advantages and disadvantages of the two decolorization technologies were compared. [Result] Both activated carbon and macroporous resin can be used for decolorization. To be specific, macroporous resin-decolorization is superior in the yield and protein retention rate, while activated carbon-decolorization is superior in decolorization rate and decolorization operation process. [Conclusion] Macroporous resin-decolorization can be used if protein is the main ingredient required in the experiment, while activated carbon-decolorization can be used if protein is not the main ingredient required.

Exploration on Separation of Periplaneta Americana Small Peptides with Tricine-SDS-PAGE System

[ Objective] This study aimed to explore a Tricine-SDS-PAGE method for separation and purification of Periplaneta Americana small peptides. [ Method] By comparing the separation effects of three types of gels with different ratios on P. Americana small peptides, an appropriate Tricine-SDS-PAGE method for separation of small peptides was established. [ Result] Standard low molecular weight Marker was preliminarily separated to a certain extent with polyacrylamide gel electraphoresis, while the sample mixture was not separated due to the different levels of aggregation in separating gel, spacer gel and stacking gel. [Condusion] More appropriate polyacrylamide gel electrephoresis separation method or other better separation methods were to be further explored for separating peptide mixture with smaller molecular weight.

Separation of Antitumor Active Plypeptides from Periplaneta Americana by Ultra Filtration Technology

[Objective] The paper was to establish ultrafiltration membrane technology for separation of antitumor active plypeptides from Periplaneta Americana. [Method] Hollow fiber ultrafiltration membrane（3 kDa）was used to separate antitumor active plypeptides from P. Americana. Through single factor test, the impact of rotation speed, concentration ratio, pH and operation time on separation effort was studied, and the separation technology conditions were further optimized through orthogonal test. [Result] The best technology conditions for separation of antitumor active plypeptides from P. Americana were as follows： rotation speed 4 500 r/min, concentration ratio 30%, pH 11, and the rotation speed was the major influencing factor. [Conclusion] The study laid foundation for industrialization of ultrafiltration membrane separation of antitumor active plypeptides from P. Americana.

Periplaneta americana extract promotes infectious diabetic ulcers wound healing by downregulation of LINC01133/SLAMF9

Wound healing in diabetic ulcers remains a significant clinical challenge,primarily due to bacterial infection and impaired angiogenesis.Periplaneta americana extract(PAE)has been widely used to treat diabetic wounds,yet its underlying mechanisms are not fully understood.This study aimed to elucidate these mechanisms by analyzing long non-coding RNA(lncRNA)expressions in the wound tissues from diabetic anal fistula patients treated with or without PAE,using high-throughput sequencing.Peripheral blood monocytes from patients were differentiated into M0 macrophages with human macrophage colony-stimulating factor(hMCSF)and subsequently polarized into M1 macrophages with lipopolysaccharide.The results indicated that LINC01133 and SLAMF9 were downregulated in wound tissues of patients treated with PAE.Furthermore,PAE suppressed M1 macrophage polarization and enhanced human umbilical vein endothelial cell(HUVEC)proliferation,migration,and angiogenesis.These effects were diminished when LINC01133 or SLAMF9 were overexpressed.Mechanistically,LINC01133 was shown to upregulate SLAMF9 through interaction with ELAVL1.Overexpression of SLAMF9 reversed the effects of LINC01133 silencing on macrophage polarization and HUVEC functions.In conclusion,PAE facilitates the healing of infected diabetic ulcers by downregulating the LINC01133/SLAMF9 pathway.

Protective effect of periplaneta americana extract on intestinal mucosal barrier function in patients with sepsis

OBJECTIVE： To investigate the effect of the periplaneta americana extract on the intestinal mucosal barrier and prognostic implications in patients with sepsis. METHODS： Sixty and six patients with sepsis were assigned randomly to treatment group （32 cases） and control group （32 cases）. The extractfrom periplaneta americana plus conventional medication for sepsis was administered to the treatment group, while the control group only received conventional treatment. The gastrointestinal function scores and acute physiology and chronic health evaluation II （APACHE 11 ） scores of all subjects were documented at baseline, at days 1, 3 and 7 after treatment respectively and their blood endotoxin was tested at the same time points as well. The incidence of death was recorded for both groups throughout the trial. RESULTS： At days 3 and 7 after treatment, gastrointestinal function score, APACHE II, and endotoxin level in treatment group wasbetter than that in con- trol group and the difference between them was significant （both P〈0~05）. Although the incidence of death in treatment group was less than that in control group, the difference between the two groups was not significant （P〉 0.05）. CONCLUSION： The extract of periplaneta ameri- cana had protective effect on intestinal mucosal barrier and could improve the condition and prog- nosis in patients with sepsis.

Molecular and functional characterization of the American cockroach, Periplaneta americana, Rab5： the first exopterygotan low molecular weight ovarian GTPase during oogenesis

The small Rab GTPases are key regulators of membrane vesicle trafficking. Ovaries of Periplaneta americana （Linnaeus） （Blattodea： Blattidae） have small molecular weight GTP/ATP-binding proteins during early and late vitellogenic periods of oogenesis. However, the identification and characterization of the detected proteins have not been yet reported. Herein, we cloned a cDNA encoding Rab5 from the American cockroach, P. americana, ovaries （PamRab5）. It comprises 796 bp, encoding a protein of 213 amino acid residues with a predicted molecular weight of 23.5 kDa. PamRab5 exists as a single-copy gene in the P. americana genome, as revealed by Southern blot analysis. An approximate 2.6 kb ovarian mRNA was transcribed especially at high levels in the previtellogenic ovaries, detected by Northern blot analysis. The muscle and head tissues also showed high levels of PamRab5 transcript. PamRab5 protein was localized, via immunofluorescence labeling, to germline-derived cells of the oocytes, very early during oocyte differentiation. Immunoblotting detected a ~25 kDa signal as a membrane-associated form revealed after application of detergent in the extraction buffer, and 23 kDa as a cytosolic form consistent with the predicted molecular weight from amino acid sequence in different tissues including ovary, muscles and head. The PamRab5 during late vitellogenic periods is required to regulate the endocytotic machinery during oogenesis in this cockroach. This is the first report on Rab5 from a hemimetabolan, and presents an inaugural step in probing the molecular premises of insect oocyte endocytotic trafficking important for oogenesis and embryonic development.

一测多评法测定美洲大蠊药材5种核苷类成分

目的建立高效液相色谱(HPLC)一测多评法同时测定美洲大蠊中尿嘧啶、尿苷、次黄嘌呤、肌苷及鸟苷5种成分的含量。方法采用Agilent ZORBAX SB-Aq色谱柱(250 mm×4.6 mm,5μm);以甲醇-0.01 mol·L^(-1)磷酸二氢钾溶液为流动相,梯度洗脱;流速:1.0 mL·min^(-1);柱温:20℃;检测波长:260 nm;进样量:10μL。计算内参物尿苷与其他成分的相对校正因子(fa/b)。一测多评法测定10批美洲大蠊中5种成分含量,并与外标法比较。结果5种核苷类在各自范围内线性良好(r>0.9995),平均加样回收率97.0%~100.8%。尿嘧啶、次黄嘌呤、肌苷、鸟苷的fa/b分别是0.9080、1.0053、1.9695、1.3034。结论所建立的方法准确稳定,可为美洲大蠊药材及提取物的质量控制提供理论参考。

美洲大蠊肠道菌株WA5-1-7的初步鉴定及其抗菌活性成分的研究

目的对具有抗白念珠菌活性的美洲大蠊肠道来源菌株WA5-1-7进行形态学和分子生物学鉴定,并对其次级代谢物进行分离纯化,从中筛选具有抗菌活性的单体化合物。方法采用染色法和扫描电子显微镜观察菌株形态;利用PCR技术扩增菌株16S rRNA,通过其基因序列与NCBI数据库进行BLAST比对并采用N-J法构建系统发育树。菌株发酵后经乙酸乙酯溶剂萃取获得粗提物,粗提物经硅胶柱层析、葡聚糖凝胶柱层析、HPLC分离纯化,获得单体化合物,经核磁共振、质谱数据与文献比对鉴定化合物结构,并检测化合物的抗菌活性。结果菌株WA5-1-7初步鉴定为环圈链霉菌(Streptomyces anulatus),从粗提物中分离出10个化合物,分别为SF2738F(1)、SF2738D(2)、环(脯氨酸-亮氨酸)二肽(3)、SF2738A(collismycin A,4)、环(苯丙氨酸-脯氨酸)二肽(5)、SF2738C(6)、1H-吲哚甲醛(7)、大豆黄酮(8)、放线菌素X2(9)、放线菌素D(10),其中化合物4、9和10有较强抗菌活性。结论本研究从美洲大蠊肠道来源链霉菌次级代谢产物中分离出具有抗菌活性的单体化合物,为深入探索美洲大蠊肠道菌奠定了基础。

荧光分析法测定美洲大蠊中多肽含量研究

荧光分析法是一种经济快速的分析方法,具有灵敏度高、选择性好、操作简单等优点。鉴于美洲大蠊多肽(DPSFNSWG-NH_(2))自身具有荧光的特性,通过实验建立一种美洲大蠊多肽(DPSFNSWG-NH_(2))的荧光检测新方法。实验结果表明,在最佳条件下,美洲大蠊多肽(DPSFNSWG-NH_(2))在0.12~31.25μg/mL浓度内与荧光强度之间具有良好的线性关系,线性回归方程为y=10.698x+14.625,R^(2)=0.994 2,检测限为0.09μg/mL,将所建立的新方法用于美洲大蠊药材中其多肽(DPSFNSWG-NH_(2))的测定,回收率为85.50%~89.47%。本研究方法操作简便、快速、准确,具有良好的分析应用前景,可用于美洲大蠊中DPSFNSWG-NH_(2)多肽的含量测定。

Fe_(3)O_(4)@ZIF-8-表面辅助激光解吸/电离质谱法检测美洲大蠊多肽

本实验在室温下合成了以Zn作为金属中心,2-甲基咪唑作为连接剂的磁性金属有机框架材料Fe_(3)O_(4)@ZIF-8,以Fe_(3)O_(4)@ZIF-8作为固体基质,通过建立Fe_(3)O_(4)@ZIF-8-表面辅助激光解吸/电离质谱(SALDI-MS)法,实现了美洲大蠊小分子多肽IPMTAPGL-NH_(2)、LTAPGL-NH_(2)、SLMTGPGL-NH_(2)、SLHTAPGL-NH_(2)的快速检测。实验优化了基质浓度、检测限和点样方法等条件,考察了该基质对美洲大蠊多肽IPMTAPGL-NH_(2)的质谱信号影响。与传统基质辅助激光解析电离质谱法(MALDI-MS)相比,Fe_(3)O_(4)@ZIF-8-SALDI-MS法检测背景更干净,目标多肽响应更强,检测灵敏度更高。该法可应用于血样中美洲大蠊多肽IPMTAPGL-NH_(2)的快速检测。

美洲大蠊抗菌肽的提取及其对不同致病菌抑菌活性的影响

本试验旨在探究美洲大蠊抗菌肽的最佳提取时间及其对不同致病菌抑菌活性的影响。选取100只5龄美洲大蠊,随机分为2组,每组5个重复,每个重复10只。试验以大肠杆菌为诱导源诱导美洲大蠊产生抗菌肽,诱导组注射1μL 1×10^(8) CFU/kg的大肠杆菌,对照组注射1μL的生理盐水,分别在诱导后12、24、36、48、60和72 h提取美洲大蠊抗菌肽。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)试验确定美洲大蠊抗菌肽的分子质量,通过BCA试剂盒测定美洲大蠊抗菌肽提取液的蛋白浓度,通过药敏片法测定美洲大蠊抗菌肽提取液的抑菌活性,同时测定其最小抑菌浓度(MIC)、热稳定性、pH稳定性和反复冻融稳定性。结果表明:1)美洲大蠊抗菌肽的分子质量在7.5 ku左右。诱导后12、24、36、48、60、72 h,诱导组美洲大蠊抗菌肽提取液的蛋白浓度极显著高于对照组(P<0.01),诱导后48 h时美洲大蠊抗菌肽提取液的蛋白浓度最高,因此选择48 h时诱导产生的美洲大蠊抗菌肽进行后续的抑菌试验。2)与对照组相比,诱导组的大肠杆菌、沙门氏菌和金黄色葡萄球菌的抑菌圈直径极显著升高(P<0.01)。美洲大蠊抗菌肽对大肠杆菌的最小抑菌浓度为1.0 mg/L,对沙门氏菌的最小抑菌浓度为3.0 mg/L,对金黄色葡萄球菌的最小抑菌浓度为2.5 mg/L。3)与对照组相比,经75、85、95、100℃水浴加热后的美洲大蠊抗菌肽提取液的抑菌直径显著降低(P<0.05),冻融2、4、6、8、10次的美洲大蠊抗菌肽提取液的抑菌圈直径显著降低(P<0.05),pH为1、3、7、9、11溶液处理后的美洲大蠊抗菌肽提取液的抑菌圈直径显著降低(P<0.05)。综上所述,大肠杆菌诱导后48 h为美洲大蠊抗菌肽的最佳提取时间,同时具备浓度高、热稳定性强和耐酸碱等特性。

美洲大蠊研粉干预脊髓半横断大鼠运动功能和自噬蛋白Beclin-1的表达

背景:脊髓损伤有氧化应激、炎症、凋亡、自噬等机制参与其中,激活自噬可改善脊髓损伤后的神经运动功能,对脊髓起保护作用。目的:探讨美洲大蠊研粉对脊髓半横断损伤大鼠后肢运动功能和损伤区域自噬蛋白Beclin-1的影响。方法:取30只6-8周龄SD大鼠,采用随机数字表法分为3组,每组10只:假手术组仅打开椎板,暴露脊髓后缝合;生理盐水组与美洲大蠊组均建立左侧脊髓半横断损伤模型,术后第1天开始分别灌胃给予生理盐水与美洲大蠊研粉,连续给药14 d。术后6 h及1,3,7,14 d行BBB评分,观察后肢运动功能的情况;给药14 d后处死大鼠,采用免疫组化染色、Western Blot检测、免疫荧光染色检测脊髓半横断损伤区域自噬蛋白Beclin-1的表达情况。结果与结论:①随着术后时间的延长,生理盐水组、美洲大蠊组大鼠BBB评分逐渐升高;与假手术组比较,生理盐水组、美洲大蠊组术后6 h及1,3,7,14 d的BBB评分均降低(P<0.05);美洲大蠊组术后7,14 d的BBB评分高于生理盐水组(P<0.05);②免疫组化染色显示,假手术组Beclin-1呈弱阳性表达,主要位于胞浆;生理盐水组Beclin-1主要位于胞浆,可见部分核膜表达;美洲大蠊组Beclin-1主要位于胞浆,可见部分核膜表达;生理盐水组、美洲大蠊组Beclin-1阳性细胞比例多于假手术组(P<0.05),美洲大蠊组Beclin-1阳性细胞比例多于生理盐水组(P<0.05);③Western Blot检测与免疫荧光染色显示,生理盐水组、美洲大蠊组Beclin-1蛋白表达高于假手术组(P<0.05),美洲大蠊组Beclin-1蛋白表达高于生理盐水组(P<0.05);④结果表明,美洲大蠊研粉能够改善脊髓半横断损伤大鼠的后肢运动功能,机制可能是美洲大蠊研粉中的多糖类物质增加了自噬蛋白Beclin-1的表达。

美洲大蠊提取物对便秘模型小鼠的影响

目的观察不同剂量美洲大蠊提取物对慢传输型便秘模型小鼠排便功能的影响。方法35只雄性C57BL/6J小鼠,随机分为对照组、模型组及美洲大蠊提取物高、中、低剂量组,每天给予盐酸洛哌丁胺灌胃造模及不同浓度的美洲大蠊提取物进行干预治疗,记录各组小鼠给药后粪便排泄数量及湿重;在实验结束前,收集各组小鼠粪便,采用16S rRNA分析其肠道菌群变化;同时测定各组小鼠首次排红便时间及小肠推进率。结果与对照组相比,模型组小鼠的粪便湿重、粒数,肠道推进率均降低;而排红便时间延长(P<0.01)。与模型组相比,各美洲大蠊治疗组小鼠的粪便湿重、粒数及小肠推进率增加,而排红便时间降低(P<0.05或P<0.01)。16S rRNA分析显示美洲大蠊提取物可明显提升便秘小鼠肠道粪便中Lachnospiraceae NK4A136和酸性拟杆菌、Alistipes、Rikenella microfusus DSM 15922细菌的丰度。结论美洲大蠊提取物能够缓解盐酸洛哌丁胺诱导的小鼠便秘,其作用机制与调节肠道菌群有关。

美洲大蠊粪便中微生物的分离和鉴定及其抗菌活性分析

【目的】探究美洲大蠊Periplaneta americana粪便中具有抗菌活性的真菌和放线菌,为抗菌药物的开发提供微生物来源。【方法】利用稀释涂布法与选择培养法,从云南省大理白族自治州巍山县美洲大蠊养殖场收集的美洲大蠊成虫粪便中分离纯化放线菌和真菌;采用牛津杯琼脂扩散法检测菌株代谢产物对6种病原细菌大肠杆菌Escherichia coli、粪肠球菌Enterococcus faecalis、金黄色葡萄球菌Staphylococcus aureus、耐甲氧西林的金黄色葡萄球菌(methicillin-resistant S.aureus)、铜绿假单胞菌Pseudomonas aeruginosa和鼠伤寒沙门氏菌Salmonella typhimurium的抗菌活性;通过16S rDNA和ITS rDNA基因测序鉴定抗菌活性菌株,并利用邻接法构建抗菌活性菌株系统发育树。【结果】从美洲大蠊成虫粪便中分离出真菌17株,放线菌19株。共有6株真菌和12株放线菌表现出不同程度的抗菌活性,其中真菌EPAF15-EPAF17及放线菌EPAA12和EPAA17对大肠杆菌、金黄色葡萄球菌、耐甲氧西林的金黄色葡萄球菌、铜绿假单胞菌和鼠伤寒沙门氏菌表现出较为广谱的抗菌活性。系统发育分析显示,具有抗菌活性的真菌隶属于镰刀菌属Fusarium、生丝毕赤酵母属Hyphopichia和丝孢酵母属Trichosporon;具有抗菌活性的放线菌隶属于链霉属Streptomyces。【结论】美洲大蠊成虫粪便中具有种类丰富的可培养微生物,其中的抗菌活性真菌和放线菌的发现不仅能作为进一步深入挖掘新型抗菌活性天然药物的重要微生物储备资源,也为特境微生物资源的充分利用提供了新的思路。

美洲大蠊糖蛋白对2种益生菌生长及其代谢产物的影响

目的:本实验旨在探究美洲大蠊糖蛋白(PAG)对植物乳杆菌和青春双歧杆菌生长及其代谢产物的影响。方法:在含不同浓度PAG的培养基(MRS培养基中PAG浓度分别为0.5、1.0、2.0、4.0 mg/mL;TPY培养基PAG浓度分别为1.0、2.0、4.0、8.0 mg/mL)中培养有益菌,并以菊粉为阳性对照,测定菌液的OD600值、pH以及代谢产物乳酸、短链脂肪酸(SCFAs,即乙酸、丙酸和丁酸)的含量。结果:PAG添加量达1.0 mg/mL时,促植物乳杆菌生长达到最佳,而青春双歧杆菌生长达到最佳时PAG浓度为2.0 mg/mL。添加PAG;1.0 mg/mL)的植物乳杆菌菌液的OD600值、乳酸和SCFAs产量较同浓度菊粉升高,而pH显著(P<0.05)降低。添加PAG(2.0 mg/mL)的青春双歧杆菌菌液的OD600值、丙酸和丁酸产量较同浓度菊粉升高,乳酸产量12 h优于菊粉,乙酸产量24 h优于菊粉,而pH降低,提示PAG促青春双歧杆菌产乳酸和SCFAs的效果与菊粉不相同。结论:PAG能够促进植物乳杆菌和青春双歧杆菌的增殖和有机酸量的生成,效果优于菊粉。