Inhibiting Effect of Lobenzarit Disodium on the Inter-leukin-1 Activity in Normal and in Adjuvant ArthritisKats and on the H_2O_2 Release from Rat Peritoneal Macro-phages

The effects of Lobenzarit disodium (CCA) upon the activity of interleukin-1(IL-1) of peritoneal macrophages(PMΦ)in normal and in adjuvant arthritis AA) rats and on the release of hydrogen peroxide(H_2O_2)of rat peritoneal macrophages were studied. CCA 10～200 μg/ml could inhibit IL-1activity in normal rat in vitro;CCA 10 and 50 mg/kg could depress the level of increased IL-1 in AA rats in vivo;CCA 5～100μg/ml could inhibit the release of H_2O_2 from rat PMΦin vitro.The results suggest that these effects could be one of the mechanisms of anti-inflammatory action of CCA.

Characteristic changes in astrocyte properties during astrocyte-to-neuron conversion induced by NeuroD1/Ascl1/Dlx2

Direct in vivo conversion of astrocytes into functional new neurons induced by neural transcription factors has been recognized as a potential new therapeutic intervention for neural injury and degenerative disorders. However, a few recent studies have claimed that neural transcription factors cannot convert astrocytes into neurons, attributing the converted neurons to pre-existing neurons mis-expressing transgenes. In this study, we overexpressed three distinct neural transcription factors––NeuroD1, Ascl1, and Dlx2––in reactive astrocytes in mouse cortices subjected to stab injury, resulting in a series of significant changes in astrocyte properties. Initially, the three neural transcription factors were exclusively expressed in the nuclei of astrocytes. Over time, however, these astrocytes gradually adopted neuronal morphology, and the neural transcription factors was gradually observed in the nuclei of neuron-like cells instead of astrocytes. Furthermore,we noted that transcription factor-infected astrocytes showed a progressive decrease in the expression of astrocytic markers AQP4(astrocyte endfeet signal), CX43(gap junction signal), and S100β. Importantly, none of these changes could be attributed to transgene leakage into preexisting neurons. Therefore, our findings suggest that neural transcription factors such as NeuroD1, Ascl1, and Dlx2 can effectively convert reactive astrocytes into neurons in the adult mammalian brain.

Evaluation of the <i>BRCA</i>1/2 mutation as a prognostic marker in primary peritoneal serous cancer

Introduction: The present study was a retrospective investigation of the relation between immunohistochemical BRCA1/2 status and prognosis in patients with primary peritoneal serous cancer (PPSC). Materials and Methods: We retrospectively evaluated 14 consecutive patients diagnosed with PPSC other than hereditary breast and ovarian cancer between 2005 and 2010. All patients had serum CA125 levels >40 U/mL prior to starting first-line chemotherapy with paclitaxel and carboplatin. Paclitaxel was administered as a 3-hour intravenous infusion at a dose of 175 mg/m2 on day 1, and carboplatin was delivered at an area under the curve of 5 based on the Calvert method. Patients received six cycles of first-line chemotherapy, except patients whose disease was determined to be progressive during the chemotherapy regimen. BRCA1/2 and p53 protein expression was determined by immunohistochemistry of patient tissue samples. The Cox proportional hazards model was used to evaluate univariate and independent multivariate associations with the effect of clinical parameters, such as age at diagnosis;tumor histology;tumor grade;and rate of change in CA125, and BRCA1/2, p53 status on overall survival. Probability values of less than 0.05 were considered to indicate statistical significance. Results: Two cases (14%) had the BRCA1 mutation, and none had the BRCA2 mutation. Eleven cases (79%) were positive for p53. In the univariate analysis, factors significantly associated with overall survival were (pre-chemotherapy CA125-pre-2nd chemotherapy CA125)/pre-chemotherapy CA125 (p = 0.0034) and (pre-chemotherapy CA125-pre-3rd chemotherapy CA125)/pre-chemotherapy CA125 (p = 0.0245). BRCA1 and p53 status were not predictors of overall survival. Multivariate analysis performed with overall survival as an endpoint revealed that none of the factors examined was significant. Median survival rate of patients without a BRCA1 mutation was 23.5 months (2 - 82 months), and all died. By contrast, one patient with a BRCA1 mutation remains alive at 85 months, and the other patient died at 64 months. Conclusion: BRCA1 might be a predictor of overall survival in patients with PPSC receiving chemotherapy.

Aquaporin-1蛋白在缺血心肌中表达变化及其与心肌水肿的关系

目的在动物心肌梗塞模型中探讨心肌缺血状态下心肌细胞膜水通道蛋白Aquaporin-1的变化趋势及其与心肌水肿的联系。方法采用抗AQP1特异性免疫组化考查AQP1在心肌组织中的分布特点;以Real-timePCR及Westernblotting了解在缺血状态下心肌组织AQP1基因转录及蛋白表达的动态变化趋势;采用Evan’blue定量测定法了解缺血心肌组织中毛细血管通透性的变化及用心肌水含量测定法了解缺血心肌的水肿程度。结果AQP1在心肌中存在着广泛分布,并主要分布于心肌的血管及心肌细胞膜。缺血造成AQP1在心肌的转录及蛋白表达呈现先减后增的趋势;缺血后心肌水肿程度存在着双峰样变化趋势而且AQP1蛋白的表达增加的时间特征上与缺血后的第二个水肿高峰的变化趋势相一致。结论AQP1蛋白在心肌缺血后存在着先减后增的动态变化,AQP1蛋白的表达增加可能与部分地联系到缺血后的心肌水肿的形成。

缺血预适应后心肌水肿与水通道Aquaporin-1蛋白表达变化的关系

目的通过对缺血预适应动物模型与单纯心肌缺血动物模型之间的对照研究,探讨预适应对心肌细胞膜水通道蛋白Aquaporin-1表达的影响及与心肌水肿的关系。方法以Real-time PCR及Western blotting了解在心肌组织AQP1基因转录及蛋白表达的动态变化趋势;采用Evan blue定量测定法了解心肌组织中微血管通透性的变化及用心肌水含量测定法了解缺血心肌的水肿程度。结果与缺血对照组相似,缺血预适应后心肌AQP1基因及其蛋白的表达呈现先减后增的趋势,但增加的程度较对照组为高。预适应后组织血管通透性增高明显受到抑制,但仅伴随着缺血后早期阶段心肌水肿的减轻。与微血管通透性的持续降低形成对比的同时,心肌水肿状态在之后一段时期内保持较高水平与AQP1蛋白表达增高在时间关系上相对应。结论预适应早期心肌水肿的减轻与血管通透性的抑制有关,而AQP1蛋白表达的增加可能是心肌水肿状态维持的因素。

Aquaporin-1在大鼠睾丸输出小管的免疫组织化学定位研究

目的 研究正常大鼠睾丸输出小管上皮细胞上Aquaporin 1(AQP 1)的定位分布以期了解其在水重吸收上的作用机制。方法 对正常Wistar大鼠睾丸输出小管进行常规免疫组织化学方法染色观察。结果 在睾丸输出小管非纤毛上皮细胞的刷状缘及基侧部AQP 1阳性表达强烈 ,核上区的胞内体的质膜上也有阳性表达 ;纤毛上皮细胞的纤毛亦呈阳性反应。结论 Aquaporin 1可能与睾丸输出小管非纤毛上皮细胞水重吸收功能有密切关系。

Aquaporin-1 mRNA在人非小细胞肺癌组织中的表达和意义

目的:探讨Aquaporin-1(AQP1)mRNA在人非小细胞肺癌组织中的表达和意义。方法:以相应患者手术切除的正常肺组织为对照,采用半定量RT-PCR技术检测人非小细胞肺癌(17例鳞癌和18例腺癌)组织中AQP1 mRNA的表达水平。结果:人肺腺癌组织中AQP1 mRNA表达水平为0.419±0.135,高于正常肺组织中的表达水平(0.250±0.124),二者之间差异有统计学意义(P<0.05);人肺鳞癌组织中AQP1 mRNA表达水平为0.341±0.099,与正常肺组织中AQP1 mRNA表达水平(0.264±0.114)之间无统计学差异(P>0.05)。结论:AQP1mRNA在人肺腺癌组织中表达增加,提示AQP1可能与人肺腺癌的发生和发展有关系。

维持性腹膜透析患者血清CHI3L1、HSP60与钙磷代谢、冠状动脉钙化的关系

目的探讨维持性腹膜透析患者血清壳多糖酶3样蛋白1(CHI3L1)、热休克蛋白60(HSP60)与钙磷代谢、冠状动脉钙化的关系。方法选取2021年1月至2023年1月在周口市中心医院接受持续性非卧床腹膜透析或日间非卧床腹膜透析治疗>3个月的98例维持性腹膜透析患者作为研究对象,根据是否存在钙磷代谢异常分别分入钙磷正常组与钙磷异常组,根据多层螺旋CT结果参照Agatston评分法计算其冠状动脉钙化积分(CACS),将98例维持性腹膜透析患者再次分入未钙化组与钙化组,检测并对比两组患者的血清CHI3L1、血清HSP60水平。采用Pearson相关分析探讨血清CHI3L1、HSP60与钙磷代谢指标的关系,采用受试者工作特征(ROC)曲线评估血清CHI3L1、HSP60对维持性腹膜透析患者冠状动脉钙化的评估价值。通过多因素logistic逐步回归分析探讨维持性腹膜透析患者冠状动脉钙化的影响因素。结果98例维持性腹膜透析患者分为钙磷正常组(43例)、钙磷异常组(55例),钙磷代谢异常组患者血清CHI3L1、HSP60水平高于钙磷代谢正常组患者(P<0.05)。维持性腹膜透析患者血清CHI3L1、HSP60与钙磷乘积呈正相关(P<0.05)。根据多层螺旋CT结果参照Agatston评分法计算其CACS积分,将98例维持性腹膜透析患者分为未钙化组(37例)、钙化组(61例),钙化组患者血清CHI3L1、HSP60水平高于未钙化组患者(P<0.05)。血清CHI3L1、HSP60评估维持性腹膜透析患者预后的AUC分别为0.741、0.826,两者联合评估的AUC为0.915。钙化组患者低蛋白血症、高磷饮食人数及透析龄高于未钙化组(P<0.05)。多因素logistic回归分析显示:高磷饮食、透析龄、CHI3L1、HSP60是影响维持性腹膜透析患者冠状动脉钙化的危险因素(P<0.05)。结论血清CHI3L1、HSP60在钙磷代谢异常、发生冠状动脉钙化的维持性腹膜透析患者中均呈高表达,及早检测血清CHI3L1、HSP60水平有助于及时防治冠状动脉钙化。

Advances in the management of peritoneal mesothelioma

Malignant peritoneal mesothelioma(PM) is an infrequent disease which has historically been associated with a poor prognosis. Given its long latency period and non-specific symptomatology, a diagnosis of PM can be suggested by occupational exposure history, but ultimately relies heavily on imaging and diagnostic biopsy. Early treatment options including palliative operative debulking, intraperitoneal chemotherapy, and systemic chemotherapy have marginally improved the natural course of the disease with median survival being approximately one year. The advent of cytoreduction(CRS) with hyperthermic intraperitoneal chemotherapy(HIPEC) has dramatically improved survival outcomes with wide median survival estimates between 2.5 to 9 years; these studies however remain largely heterogeneous, with differing study populations, tumor biology, and specific treatment regimens. More recent investigations have explored extent of cytoreduction, repeated operative intervention, and choice of chemotherapy but have been unable to offer definitive conclusions. CRS and HIPEC remain morbid procedures with complication rates ranging between 30% to 46% in larger series. Ac-cordingly, an increasing interest in identifying molecular targets and developing targeted therapies is emerging. Among such novel targets is sphingosine kinase 1(SphK1) which regulates the production of sphingosine-1-phosphate, a biologically active lipid implicated in various cancers including malignant mesothelioma. The known action of specific SphK inhibitors may warrant further exploration in peritoneal disease.

Effect of Dexamethasone and Aquaporin-1 Antisense Oligonucleotides on the Aquaporin-1 Expression in Cultured Human Trabecular Meshwork Cells

The changes in the expression of aquaporin-1 （AQP1） mRNA and protein in cultured human trabecular meshwork （HTM） cells treated with dexamethasone and transfected with antisense oligonucleotides （AS-ODN） were studied, and the implication of AQP1 regulation in corticosteroid-glaucoma and the possibility of AS-ODN inhibiting the AQP1 expression were evaluated. The cultured HTM cells in vitro were treated with different concentrations of dexamethasone and transfected with oligonucleotides for 5 days respectively. Then, total RNA and protein of HTM cells were extracted. The changes of AQP1 mRNA and protein were demonstrated qualitatively and quantitatively by RT-PCR and Western blot. Band intensities were detected by imaging analysis. There was a parallel relationship between the results of RT-PCR and those of Western blot. The expression levels of AQP1 mRNA and protein in dexamethasone-treated groups were increased initially and decreased later as dexamethasone concentration was stepped up. In the 0.04 μg/mL and 0.4 μg/mL groups, the levels of AQP1 were higher than in control group （0 μg/mL）. In the 4 μg/ mL and 40 μg/mL groups, the AQP1 expression levels were lower than in control group. AS-ODN could down-regulate the expression of AQP1 mRNA and protein in a dose-dependent manner. At 5 μg/mL, down-regulation efficiency reached the maximum. There was no statistically significant difference in the expression of AQP1 mRNA and protein between all sense oligonucleotides groups and control group. It was suggested that dexamethasone may induce the changes of the AQP1 expression in HTM cells to be involved in the occurrence of corticosteroid-glaucoma. AS-ODN can down-regulate the AQP1 expression in HTM cells to some extent.

Analysis of monocyte chemotactic protein-1 gene polymorphism in patients with spontaneous bacterial peritonitis

AIM:To investigate a genetic polymorphism of the monocyte chemotactic protein-1 (MCP-1 ) gene in patients with spontaneous bacterial peritonitis (SBP).METHODS:MCP-1 genotyping was performed in 23 patients with SBP and 83 cirrhotic control patients with non-infected ascites.RESULTS:The frequency of carriers of the G-allele was lower in SBP patients but this difference did not reach statistical significance. However,in the subgroup of patients with alcoholic cirrhosis (n=80),carriers of the G-allele were significantly less frequent in SBP-patients (38.1%) than in cirrhotic controls (67.8%,P=0.021). CONCLUSION:In patients with alcoholic liver cirrhosis,the-2518 MCP-1 genotype AA is a risk factor for the development of SBP.

Molecular detection of monocyte chemotactic protein-1 polymorphism in spontaneous bacterial peritonitis patients

AIM: To investigate the association of the functional monocyte chemotactic protein-1 (MCP-1) promoter polymorphism (A-2518G) with spontaneous bacterial peritonitis (SBP).

Aquaporin-1 down regulation associated with inhibiting cell viability and inducing apoptosis of human lens epithelial cells

AIM：To investigate the role of Aquaporin-1（AQP-1）in lens epithelial cells（LECs）and its potential target genes.AQP-1 is specifically expressed in LECs of eyes and is significant for lens homeostasis and transparency maintenance.Herein,AQP-1 expression in LECs was investigated to evaluate its influence on cell survival in association with its potential role in cataract formation.·M ETHODS：LECs were transfected with lentivirus carrying AQP-1 small interfering RNA（si RNA）.Real-time polymerase chain reaction（PCR）and Western blotting were conducted to detect AQP-1 expression in LECs from different groups.Meanwhile,cell counting kit-8（CCK-8）assay and flow cytometry were performed to measure LEC proliferation and apoptosis,respectively.·RESULTS：AQP-1 expression was significantly reduced in LECs,both at m RNA and protein levels（〈0.05）,after si RNA treatment.Decreased cell viability was detected by CCK-8 assay in LECs with si RNA interference,compared to control cells（〈0.05）.The apoptosis rate significantly increased in cells after si RNA interference（〈0.05）.·CONCLUSION：The decreased cell viability following AQP-1 down regulation is largely due to its induction of apoptosis of LECs.AQP-1 reduction might lead to changes of physiological functions in LECs,which might be associated with the occurrence and development of cataracts.

Monocyte chemotactic protein-1 gene polymorphism and spontaneous bacterial peritonitis

I read with great interest the article by Gbele et al published in issue 44 of World J Gastroenterol 2009.The results of their study indicate that-2518 Monocyte chemotactic protein-1(MCP-1)genotype AA is a risk factor for spontaneous bacterial peritonitis in patients with alcoholic cirrhosis.However,there are some items that need to be discussed.

Primary peritoneal carcinoma metastasizing to breast: a single case report and literature review from clinic to biology

Primary peritoneal carcinoma(PPC) is a type of rare malignant epithelial tumor. Metastasis from PPC to breast has been rarely reported. PPC originates de novo from the peritoneal tissues rather than invasion or metastasis from adjacent or remote organs.PPCs have been implicated in many cases of carcinomas of unknown primary origin. It is similar to ovarian cancer(Ov Ca),because it shares the same common embryonic origin, the coelomic epithelium(mesodermal origin). The mechanism of oncogenesis remains elusive. In this article, we report a rare case of PPC in a patient 10 years after total abdominal hysterectomy and bilateral salpingooophorectomy for uterine leiomyoma, which was widely spread in the abdomen and metastasized to the colon, liver and distant organs including breast. The treatment is similar to that of primary ovarian cancer. We also reviewed the primary peritoneal cancer metastatic to breast and discuss the possible mechanisms and biology of primary peritoneal cancer,using experimental and animal model.

A case of scirrhous gastric cancer with peritonitis carcinomatosa controlled by TS-1~ + paclitaxel for 36 mo after diagnosis

A 34-year-old female complaining of abdominal fullness was diagnosed as scirrhous gastric cancer （type 4＇） with peritonitis carcinomatosa in July 2002. A combined chemotherapy regimen was selected to control massive ascites; TS-1 80 mg/m^2 was given orally on d 1-14, 22-35, and paclitaxel 50 mg/m^2 was administered intravenously on d 1, 8, 22 and 29. After 2 courses of this regimen, the primary tumor was markedly reduced, and ascites completely vanished. Alopecia （grade 1, since d 30）, leukocytopenia （grade 2, on d 34） and anemia （grade 2, on d 34） were the only adverse events throughout the following courses. The chemotherapy was effective for 28 mo, and then it was discontinued upon the patient＇s own request, and she survived for 36 mo after diagnosis.

Increased aquaporin-1 levels in lens epithelial cells with primary angle-closure glaucoma

AIM： To determine the levels of aquaporin-1（AQP-1） in the lens epithelial cells（LECs） of primary glaucoma and to clarify its correlation with lens thickness.METHODS： This study comprised 64 eyes of 64 patients with primary glaucoma, who were divided into 3 groups： 25 eyes of 25 patients with acute primary angle-closure glaucoma（APACG）, 19 eyes of 19 patients with chronic primary angle-closure glaucoma（CPACG） and 20 eyes of 20 patients with primary open angle glaucoma（POAG）. This study also included 12 eyes of 12 patients with senile cataract as controls. The levels of AQP-1 in LECs were examined by real-time quantitative polymerase chain reaction（RT-q PCR） and immunohistochemistry. The lens thickness was measured by A-scan ultrasonography. RESULTS： The AQP-1 m RNA levels of LECs were 0.84±0.27, 0.69±0.34, 0.44±0.19 and 0.51±0.21 in APACG, CPACG, POAG and senile cataract group, respectively. The levels of AQP-1m RNA were significantly higher in PACG groups compared with those in senile cataract and POAG group（all P〈0.05）. The immunohistochemistry showed the AQP-1 expression were strong-positive in PACG groups, but weak-positive in senile cataract and POAG group. A positive correlation was found between AQP-1 m RNA levels and the lens thickness（r=0.645, P〈0.001）. CONCLUSION： These findings show that the higher expression of AQP-1 in LECs may contribute to increased lens thickness, which might be associated with the occurrence and development of PACG.

Gene polymorphisms associated with sudden decreases in heart rate during extensive peritoneal lavage with distilled water after gastrectomy

BACKGROUND Our previous study found that the telomerase-associated protein 1(TEP1,rs938886 and rs1713449)and homo sapiens RecQ like helicase 5(RECQL5,rs820196)single nucleotide polymorphisms(SNPs)were associated with changes in heart rate(HR)≥30%during peritoneal lavage with distilled water after gastrectomy.This study established a single tube method for detecting these three SNPs using two-dimensional(2D)polymerase chain reaction(PCR),and investigated whether SNP-SNP and SNP-environment interactions increase the risk of high HR variability(HRV).AIM To investigate whether genotypes,genetic patterns,SNP-SNP and SNP-environment interactions were associated with HRV.METHODS 2D PCR was used to establish a single-tube method to detect TEP1 rs938886 and rs1713449 and RECQL5 rs820196,and the results were compared with those of sanger sequencing.After adjusting for confounders such as age,sex,smoking,hypertension,and thyroid dysfunction,a nonconditional logistic regression model was used to assess the associations between the genotypes and the genetic patterns(codominant,dominant,overdominant,recessive,and additive)of the three SNPs and a risk≥15%or≥30%of a sudden drop in HR during postoperative peritoneal lavage in patients with gastric cancer.Gene-gene and geneenvironment interactions were analyzed using generalized multifactor dimensionality reduction.RESULTS The coincidence rate between the 2D PCR and sequencing was 100%.When the HRV cutoff value was 15%,the patients with the RECQL5(rs820196)TC genotype had a higher risk of high HRV than those who had the TT genotype(odds ratio=1.97;95%CI:1.05-3.70;P=0.045).Under the codominant and overdominant models,the TC genotype of RECQL5(rs820196)was associated with a higher risk of HR decrease relative to the TT and TT+CC genotypes(P=0.031 and 0.016,respectively).When the HRV cutoff value was 30%,patients carrying the GC-TC genotypes of rs938886 and rs820196 showed a higher HRV risk when compared with the GG–TT genotype carriers(P=0.01).In the three-factor model of rs938886,rs820196,and rs1713449,patients carrying the GC-TC-CT genotype had a higher risk of HRV compared with the wild-type GG-TT-CC carriers(P=0.01).For rs820196,nonsmokers with the TC genotype had a higher HRV risk compared with nonsmokers carrying the TT genotype(P=0.04).When the HRV cutoff value was 15%,patients carrying the TT-TT and the TC-CT genotypes of rs820196 and rs1713449 showed a higher HRV risk when compared with TT-CC genotype carriers(P=0.04 and 0.01,respectively).Patients carrying the GC-CT-TC genotypes of rs938886,rs1713449,and rs820196 showed a higher HRV risk compared with GG-CC-TT genotype carriers(P=0.02).When the HRV cutoff value was 15%,the best-fitting models for the interactions between the SNPs and the environment were the rs820196-smoking(P=0.022)and rs820196-hypertension(P=0.043)models.Consistent with the results of the previous grouping,for rs820196,the TC genotype nonsmokers had a higher HRV risk compared with nonsmokers carrying the TT genotype(P=0.01).CONCLUSION The polymorphism of the RECQL5 and TEP1 genes were associated with HRV during peritoneal lavage with distilled water after gastrectomy.

Regulatory Effect of Dexamethasone on Aquaporin-1 Expression in Cultured Bovine Trabecular Meshwork Cells

To evaluate the effect of dexamethasone on the expression of aquaporin-1 （AQP-1） in cultured bovine trabeeular meshwork cells, bovine trabeeular meshwork cells were cultured in vitro and reproduced to the third and the fourth generation, then treated with dexamethasone at the concentrations of 5, 25, 50, 250μg/L respectively for 7 days. Immunohistochemical technique-supervision method was employed to measure, and image analysis system to analyze the expression of AQP-1 in normal cultured bovine trahecular meshwork cells and those treated with dexamethasone. In normal bovine trabeeular meshwork cells, the grayseale of AQP-1 positive staining was 167.94± 1.18, while it was 168.92±0.91, 176.72±1.80, 180. 64±1.31, 185.64±1.58 in cells treated with 5, 25, 50, 250μg/L concentrations of dexamethasone. When the concentration of dexamethasone was higher than 25 μg/L, the expression of AQP-1 was significantly inhibited （P〈0.05）. The regulation of AQP-1 expression by dexamethasone in cultured bovine trahecular meshwork cells in vitro may be one of causes that retard the aqueous outflow in glueoeorticoid-induced glaucoma.

血清COL10A1、TK1、MIP-3α水平与胃癌患者病理特征的关系及其对腹膜转移的诊断价值研究

目的研究血清X型胶原α1链(COL10A1)、胸苷激酶1(TK1)、巨噬细胞炎症蛋白-3α(MIP-3α)水平与胃癌患者病理特征的关系及其对胃癌腹膜转移的诊断价值。方法以2021年1月至2022年12月邢台市人民医院收治的96例胃癌患者作为恶性组,其中有腹膜转移27例,无腹膜转移69例;选取同期收治的104例胃良性病变患者作为良性病变组,选取112例健康体检者作为健康对照组。比较各组血清COL10A1、TK1、MIP-3α水平及不同病理特征、有无腹膜转移胃癌患者血清COL10A1、TK1、MIP-3α水平,采用受试者工作特征(ROC)曲线分析血清COL10A1、TK1、MIP-3α对胃癌患者腹膜转移的诊断价值。结果恶性组血清COL10A1、MIP-3α、TK1水平高于健康对照组、良性病变组,良性病变组血清COL10A1、MIP-3α水平高于健康对照组,差异均有统计学意义(P<0.05)。低/未分化、有脉管浸润、肿瘤临床病理分期(TNM)分期Ⅲ~Ⅳ期胃癌患者血清COL10A1、TK1、MIP-3α水平高于高/中分化、无脉管浸润、TNM分期Ⅰ~Ⅱ期患者(P<0.05)。有腹膜转移胃癌患者血清COL10A1、TK1、MIP-3α水平高于无腹膜转移患者(P<0.05)。血清COL10A1、TK1、MIP-3α单项及3项联合检测诊断胃癌腹膜转移的曲线下面积(AUC)分别为0.722(95%CI:0.621~0.809)、0.749(95%CI:0.651~0.832)、0.736(95%CI:0.637~0.821)、0.853(95%CI:0.766~0.917),3项联合检测诊断的AUC大于3项单独检测(Z=1.990、3.617、2.986,P<0.001)。结论胃癌的发生导致患者血清COL10A1、TK1、MIP-3α水平升高,同时随着胃癌患者病情的进展,血清COL10A1、TK1、MIP-3α水平升高,且3项指标联合检测对胃癌患者腹膜转移具有较好的诊断价值。