Rooting ability in Vitis vinifera L. is the phenomena studied. The evolution of the total lipids contents and fatty acids amount of cuttings during the rooting were determined. Total lipid extraction was carried out b...Rooting ability in Vitis vinifera L. is the phenomena studied. The evolution of the total lipids contents and fatty acids amount of cuttings during the rooting were determined. Total lipid extraction was carried out by an extraction solvent consisting of a mixture of chloroform and methanol. Methylation of fatty acids was carried out by the boron trifluoride methanol complex. Their analysis is performed by gas chromatography. We have obtained a content of merithallus lipids equivalent with then in bibliography. In other hand 3 fatty acids were obtained: eicosapentaenoic acid, oleic acid and docosanoic acid. Among the studied of rooting abilities, we observed decreased levels of total lipids and in the essential fatty acids: eicosapentaenoic acid C20:5 n-3 and oleic acid C18:1 n-9. Minor fatty acids cuttings experimental have a variable evolution. Lipids and certain fatty acids may be markers of rooting in the vine.展开更多
目的建立定量检测Per1 mRNA表达水平的系统,并检测小鼠纹状体中该基因的表达规律。方法提取小鼠纹状体总RNA,用Per1基因特异性引物进行聚合酶链反应(PCR),以荧光染料SYBR green I法进行实时检测。结果确定了Per1基因荧光定量的扩增和检...目的建立定量检测Per1 mRNA表达水平的系统,并检测小鼠纹状体中该基因的表达规律。方法提取小鼠纹状体总RNA,用Per1基因特异性引物进行聚合酶链反应(PCR),以荧光染料SYBR green I法进行实时检测。结果确定了Per1基因荧光定量的扩增和检测参数。证明在此条件下无非特异扩增,并且扩增效率在90%以上。利用此检测体系发现小鼠纹状体内Per1基因的表达呈节律性波动。结论所建立的方法能够定量测定Per1 mRNA表达水平,具有灵敏度高,线性范围广的特点。纹状体中Per1基因表达的节律性波动提示黑质纹状体系统间的相互调节可能具有昼夜节律性差异。展开更多
首先介绍了基于最新Linux内核的防火墙技术netfilter框架结构,包括包过滤防火墙、代理服务器、完全状态检测技术、NAT、DMZ的概念、原理和用途,以及在Linux中具体实现的方法。着重探讨了一个用Perl/CGI编写的集成的WebAdmin来构建一个...首先介绍了基于最新Linux内核的防火墙技术netfilter框架结构,包括包过滤防火墙、代理服务器、完全状态检测技术、NAT、DMZ的概念、原理和用途,以及在Linux中具体实现的方法。着重探讨了一个用Perl/CGI编写的集成的WebAdmin来构建一个集成的带有状态检测功能的防火墙系统,该系统可以通过WebAdmin管理界面来进行本地/远程配置管理整个防火墙的策略,并提供了包括对于ADSL服务获得的动态IP地址的支持,DNS代理的集成,日志的记录,连接追踪,并特别集成了一个PPTPVPN的功能,使得该系统成为一个all in one的防火墙系统。展开更多
文摘Rooting ability in Vitis vinifera L. is the phenomena studied. The evolution of the total lipids contents and fatty acids amount of cuttings during the rooting were determined. Total lipid extraction was carried out by an extraction solvent consisting of a mixture of chloroform and methanol. Methylation of fatty acids was carried out by the boron trifluoride methanol complex. Their analysis is performed by gas chromatography. We have obtained a content of merithallus lipids equivalent with then in bibliography. In other hand 3 fatty acids were obtained: eicosapentaenoic acid, oleic acid and docosanoic acid. Among the studied of rooting abilities, we observed decreased levels of total lipids and in the essential fatty acids: eicosapentaenoic acid C20:5 n-3 and oleic acid C18:1 n-9. Minor fatty acids cuttings experimental have a variable evolution. Lipids and certain fatty acids may be markers of rooting in the vine.
文摘目的建立定量检测Per1 mRNA表达水平的系统,并检测小鼠纹状体中该基因的表达规律。方法提取小鼠纹状体总RNA,用Per1基因特异性引物进行聚合酶链反应(PCR),以荧光染料SYBR green I法进行实时检测。结果确定了Per1基因荧光定量的扩增和检测参数。证明在此条件下无非特异扩增,并且扩增效率在90%以上。利用此检测体系发现小鼠纹状体内Per1基因的表达呈节律性波动。结论所建立的方法能够定量测定Per1 mRNA表达水平,具有灵敏度高,线性范围广的特点。纹状体中Per1基因表达的节律性波动提示黑质纹状体系统间的相互调节可能具有昼夜节律性差异。
文摘首先介绍了基于最新Linux内核的防火墙技术netfilter框架结构,包括包过滤防火墙、代理服务器、完全状态检测技术、NAT、DMZ的概念、原理和用途,以及在Linux中具体实现的方法。着重探讨了一个用Perl/CGI编写的集成的WebAdmin来构建一个集成的带有状态检测功能的防火墙系统,该系统可以通过WebAdmin管理界面来进行本地/远程配置管理整个防火墙的策略,并提供了包括对于ADSL服务获得的动态IP地址的支持,DNS代理的集成,日志的记录,连接追踪,并特别集成了一个PPTPVPN的功能,使得该系统成为一个all in one的防火墙系统。
