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Variation of peroxidase isoenzyme and biofilm of Phanerochaete chrysosporium in continuous membrane bioreactor for Reactive Brilliant Red X3-B treatment 被引量:1
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作者 GAO Shang, CHEN Cheng, TAO Fang, HUANG Minsheng, MA Lihua, WANG Zhonghua, WU Linhui School of Resource and Environment Science, East China Normal University, Shanghai 200062, China. 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2009年第7期940-947,共8页
The influence of a Reactive Brilliant Red X-3B (RBR X-3B) dye on the peroxidase isoenzyme ofPhanerochaete chrysosporium was determined, and the biofilm structure in a white rot fungal continuous membrane bioreactor ... The influence of a Reactive Brilliant Red X-3B (RBR X-3B) dye on the peroxidase isoenzyme ofPhanerochaete chrysosporium was determined, and the biofilm structure in a white rot fungal continuous membrane bioreactor (MBR) was also investigated by scanning electron microscope (SEM). The variation of peroxidase isoenzyme and the decolorization rate in the continuous MBR were evaluated. The results showed that the 100 mg/L RBR X-3B could stimulate the production of the peroxidase isoenzyme in the shaking-flask culture. In addition, two new peroxidase isoenzyme bands with relative mobility (Rf) value of 0.27 and 0.28 appeared, but the activity was lower than the blank control of 11 d. In the continuous MBR, the system worked stably during the first 60 d, the main peroxidase isoenzyme bands existed and three new bands with Rf value of 0.10, 0.27, and 0.28 appeared. Meanwhile, the biofilm grew well and the average decolorization rate could reach 90.6%. But the bands of peroxidase isoenzyme decreased rapidly at day 65, only two bands with Rf value 0.24 and 0.26 existed, and the decolorization rate decreased to 78.3%. Therefore, 5 bottles of P. chrysosporium mycelial pellet were added into the MBR, and then the activity of the peroxidase isoenzyme and the decolorization rate had a slight recovery. Finally, the decolorization rate finally decreased to 75.2%. These results contribute to a comprehensive understanding of the variation of peroxidase isoenzyme and biofilm in continuous MBR by white rot fungi. 展开更多
关键词 Phanerochaete chrysosporium continuous membrane bioreactor Reactive Brilliant Red X3-B peroxidase isoenzyme pattern
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Stimulating Effects of Seed Treatment by Magnetized Plasma on Tomato Growth and Yield 被引量:19
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作者 尹美强 黄明镜 +1 位作者 马步洲 马腾才 《Plasma Science and Technology》 SCIE EI CAS CSCD 2005年第6期3143-3147,共5页
Tomato seeds (Lycopersicon esculentum L. Mill. cv. zhongshu No. 6) were treated by magnetized plasma before being sown to investigate its effect on the growth and yield of tomatoes. Biochemical analysis showed that ... Tomato seeds (Lycopersicon esculentum L. Mill. cv. zhongshu No. 6) were treated by magnetized plasma before being sown to investigate its effect on the growth and yield of tomatoes. Biochemical analysis showed that dehydrogenase activity increased with the increase of the current but decreased when the current was higher than 1.5 A. The activities of peroxidase (POD) isoenzyme changed in the same pattern. There was no difference in germination percentage between treatments and control, which were carried out in laboratory conditions. However, significant (c~ = 0.01) difference was observed in germination percentage in the pot experiment. In the pot experiment, the sprouting rate for the treatment with a 1.5 A current was 32.75%, whereas the untreated was only 4.75% on the eleventh day. Germination time is more than one day earlier than the control. The 1.5 A treatment increased the tomato yield by 20.7%. 展开更多
关键词 magnetized plasma TOMATO dehydrogenase activity peroxidase (POD) isoenzyme GERMINATION YIELD
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RESEARCH ON THE HEREDITY AND VARIATION OF THE PERUXIDASE ISOENZYME OF NEEDLES OF PINUS KORAIENSIS
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作者 Guan WeijunNortheast Forestry University 《Journal of Northeast Forestry University》 SCIE CAS CSCD 1993年第1期69-72,共4页
Through the polyacrylamide gel clectrophoresis analysis of thirty-two needle samples of ninty-years old pinus koraiensis in Harbin, seven typies of zone and distribution of the zone frequency, and some objective fact ... Through the polyacrylamide gel clectrophoresis analysis of thirty-two needle samples of ninty-years old pinus koraiensis in Harbin, seven typies of zone and distribution of the zone frequency, and some objective fact of heredity and variation were determined. These informations can be used as reference materials in the research of heredity and variation of pinus koraiensis. 展开更多
关键词 Pinus koraiensis peroxidase isoenzyme ISOENZYME Heredity and variation
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Downregulation of the Basic Peroxidase Isoenzyme from Zinnia elegans by Gibberellic Acid 被引量:3
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作者 María Josefa López Núez-Flores Jorge Gutirrez +3 位作者 Laura V.Gómez-Ros Esther Novo Uzal Mariana Sottomayor Alfonso Ros Barceló 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2010年第2期244-251,共8页
Hypocotyl formation during the epigeal germination of seedlings is under strict hormonal regulation. In a 3 d old Zinnia elegans seedling system, gibberellic acid (GA3) exerts an opposite effect to that exerted by l... Hypocotyl formation during the epigeal germination of seedlings is under strict hormonal regulation. In a 3 d old Zinnia elegans seedling system, gibberellic acid (GA3) exerts an opposite effect to that exerted by light on hypocotyl photomorphogenesis because GAz promotes an etiolated-like growth with an inhibition of radial (secondary) growth. For this reason, the effect of GA3 on the basic peroxidase isoenzyme from Z. elegans (ZePrx), an enzyme involved in hypocotyl lignin biosynthesis, was studied. The results showed that GA3 reduces ZePrx activity, similarly to the way in which it reduces seedling secondary growth. This hormonal response is supported by the analysis of the ZePrx promoter, which contains four types of GA3-responsive cis.elements: the W Box/O2S; the Pyr Box; the GARE; and the Amy Box. Taken together, these results suggest that ZePrx is directly regulated by GA3, with this effect matching the inhibitory effect of GA on the hypocotyl secondary growth. 展开更多
关键词 GA Downregulation of the Basic peroxidase Isoenzyme from Zinnia elegans by Gibberellic Acid CIS FIGURE
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