Peroxyoxalate chemiluminescence was, for the first time, examined by using ternary mixed solutions of water-hydrophilic/hydrophobic organic solvent. Eosin Y as a model fluorescence compound was dissolved with the tern...Peroxyoxalate chemiluminescence was, for the first time, examined by using ternary mixed solutions of water-hydrophilic/hydrophobic organic solvent. Eosin Y as a model fluorescence compound was dissolved with the ternary solutions of water (1.0 mM carbonate buffer, pH 9.0)-acetonitrile-ethyl acetate, water-rich of 15:3:2 volume ratio and organic solvent-rich of 3:8:4 volume ratio, to which bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide chemiluminescence reagent were added. The chemiluminescence observed with the ternary solutions, especially the organic solvent-rich solution, showed a larger signal than that observed with the water only solution or water-acetonitrile mixed solution. Chemiluminescence in the presence of twenty types of α-amino acid was similarly examined by using the ternary organic solvent-rich solution. The chemiluminescence of three α-amino acids with fluorescence properties was enhanced with the ternary solution. The data reported here may contribute to development of a new, sensitive peroxyoxalate chemiluminescence detection system.展开更多
A novel peroxyoxalate chemiluminescence system has been designed for the determination of Triton X-100 （TX-100）, in which a hydrophobic fluorescent conjugated polymer, poly[2,5-bisnonyloxy-1,4-phenyleneethynylene-9,...A novel peroxyoxalate chemiluminescence system has been designed for the determination of Triton X-100 （TX-100）, in which a hydrophobic fluorescent conjugated polymer, poly[2,5-bisnonyloxy-1,4-phenyleneethynylene-9,10-anthrylene] （PPEA） was employed as a fluorophor. A strong enhanced intensity of chemiluminescence （CL） was observed in the presence of TX-100, due to the improved emission efficiency of PPEA in the presence of TX-100. Under optimum conditions, the detection range of Triton X-100 is between 1.0 ×10 ^-7 and 1.0 ×10 ^-4 mol·L ^-1, with a detection limit at 6.0×10 ^-8 moloL 1. The relative standard deviation is 2.4% （n=6） for 1.0×10 ^-6 mol·L ^-1Triton X-100. This method provides satisfying results in the detection of TX-100 in nature water and biological samples with high sensitivity and wide linear range.展开更多
文摘Peroxyoxalate chemiluminescence was, for the first time, examined by using ternary mixed solutions of water-hydrophilic/hydrophobic organic solvent. Eosin Y as a model fluorescence compound was dissolved with the ternary solutions of water (1.0 mM carbonate buffer, pH 9.0)-acetonitrile-ethyl acetate, water-rich of 15:3:2 volume ratio and organic solvent-rich of 3:8:4 volume ratio, to which bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide chemiluminescence reagent were added. The chemiluminescence observed with the ternary solutions, especially the organic solvent-rich solution, showed a larger signal than that observed with the water only solution or water-acetonitrile mixed solution. Chemiluminescence in the presence of twenty types of α-amino acid was similarly examined by using the ternary organic solvent-rich solution. The chemiluminescence of three α-amino acids with fluorescence properties was enhanced with the ternary solution. The data reported here may contribute to development of a new, sensitive peroxyoxalate chemiluminescence detection system.
基金Project supported by the National Natural Science Foundation of China （No. 20875003）.
文摘A novel peroxyoxalate chemiluminescence system has been designed for the determination of Triton X-100 （TX-100）, in which a hydrophobic fluorescent conjugated polymer, poly[2,5-bisnonyloxy-1,4-phenyleneethynylene-9,10-anthrylene] （PPEA） was employed as a fluorophor. A strong enhanced intensity of chemiluminescence （CL） was observed in the presence of TX-100, due to the improved emission efficiency of PPEA in the presence of TX-100. Under optimum conditions, the detection range of Triton X-100 is between 1.0 ×10 ^-7 and 1.0 ×10 ^-4 mol·L ^-1, with a detection limit at 6.0×10 ^-8 moloL 1. The relative standard deviation is 2.4% （n=6） for 1.0×10 ^-6 mol·L ^-1Triton X-100. This method provides satisfying results in the detection of TX-100 in nature water and biological samples with high sensitivity and wide linear range.
