Influences of propylene treatment on fruit softening and ethylene biosynthesis of persimmon genotype, 'Hiratanenashi' were investigated. The treatment with propylene could accelerate the softening and enhance ...Influences of propylene treatment on fruit softening and ethylene biosynthesis of persimmon genotype, 'Hiratanenashi' were investigated. The treatment with propylene could accelerate the softening and enhance ethylene biosynthesis, with higher sensitivity of immature fruit to the propylene was consistent with mRNA increase derived from transcription of ACS and ACO genes. Furthermore, ethylene synthesis increase in immature fruits was controlled and regulated mainly by DK-ACS1, DK-ACS2, DK-ACO1 and DK-ACO2, but regulated only by DK-ACS1 and DK-ACO1 in mature fruits.展开更多
以中国柿膨大期和成熟衰老期果实cDNA为模板,参照其它植物细胞壁扩展蛋白(expans in)基因序列设计兼并引物,进行RT-PCR.得到的扩增产物与pUCm-T质粒连接,转化大肠杆菌JM 109.任意挑选阳性克隆,进行序列测定,得到2个序列不同的expans in ...以中国柿膨大期和成熟衰老期果实cDNA为模板,参照其它植物细胞壁扩展蛋白(expans in)基因序列设计兼并引物,进行RT-PCR.得到的扩增产物与pUCm-T质粒连接,转化大肠杆菌JM 109.任意挑选阳性克隆,进行序列测定,得到2个序列不同的expans in cDNA片段,分别命名为Cdk-Exp1和Cdk-Exp2.2个片段均存在ex-p ansin基因的保守区域,即8个半胱氨酸残基和3个色氨酸残基.Cdk-Exp1和Cdk-Exp2分别由531个碱基和522个碱基组成,编码177个和174个氨基酸,二者核苷酸与氨基酸序列同源性分别高达92.635%和92.156%.中国柿果实expans in cDNA与草莓、番茄和桃果实的扩展蛋白基因在氨基酸水平上有较高的同源性.展开更多
基金funded by the National Natural Science Foundation of China(30170662).
文摘Influences of propylene treatment on fruit softening and ethylene biosynthesis of persimmon genotype, 'Hiratanenashi' were investigated. The treatment with propylene could accelerate the softening and enhance ethylene biosynthesis, with higher sensitivity of immature fruit to the propylene was consistent with mRNA increase derived from transcription of ACS and ACO genes. Furthermore, ethylene synthesis increase in immature fruits was controlled and regulated mainly by DK-ACS1, DK-ACS2, DK-ACO1 and DK-ACO2, but regulated only by DK-ACS1 and DK-ACO1 in mature fruits.
文摘以中国柿膨大期和成熟衰老期果实cDNA为模板,参照其它植物细胞壁扩展蛋白(expans in)基因序列设计兼并引物,进行RT-PCR.得到的扩增产物与pUCm-T质粒连接,转化大肠杆菌JM 109.任意挑选阳性克隆,进行序列测定,得到2个序列不同的expans in cDNA片段,分别命名为Cdk-Exp1和Cdk-Exp2.2个片段均存在ex-p ansin基因的保守区域,即8个半胱氨酸残基和3个色氨酸残基.Cdk-Exp1和Cdk-Exp2分别由531个碱基和522个碱基组成,编码177个和174个氨基酸,二者核苷酸与氨基酸序列同源性分别高达92.635%和92.156%.中国柿果实expans in cDNA与草莓、番茄和桃果实的扩展蛋白基因在氨基酸水平上有较高的同源性.