Since throat swab specimens obtained from three adult Chinese patients were confirmed as an avian-origin influenza A （H7N9） virus by local Centers for Disease Control and Prevention （CDC） in 2013,many confirmed ca...Since throat swab specimens obtained from three adult Chinese patients were confirmed as an avian-origin influenza A （H7N9） virus by local Centers for Disease Control and Prevention （CDC） in 2013,many confirmed cases have been reported in Mainland of China.展开更多
Background:Severe fever with thrombocytopenia syndrome virus(SFTSV)is a tick-borne bunyavirus that could cause a severe hemorrhagic fever termed SFTS with a high fatality rate of up to 30%.Importantly,SFTSV is frequen...Background:Severe fever with thrombocytopenia syndrome virus(SFTSV)is a tick-borne bunyavirus that could cause a severe hemorrhagic fever termed SFTS with a high fatality rate of up to 30%.Importantly,SFTSV is frequently transmitted from person-to-person and patients’blood or excreta are considered as the risk factors for transmission of SFTSV.However,the mechanism of person-to-person transmission of SFTSV is still elusive.Methods:In this study,wild-type(WT)C57BL/6 J mice and a lethal SFTSV mouse model IFNAR−/−A129 mice were utilized to evaluate whether SFTSV could be transmitted via oral or ocular routes.C57BL/6 J mice were inoculated with cell-cultured SFTSV via oral and ocular inoculation.IFNAR−/−A129 mice were inoculated with cell-cultured SFTSV or SFTSV infected mouse acute sera via oral and ocular inoculation.Results:We found that SFTSV antibody positive rates in C57BL/6 J mice were 70%(7/10)and 30%(3/10)in the oral inoculation group and ocular inoculation group,respectively on day 21 post SFTSV inoculation.The mortality rates of IFNAR−/−mice with oral and ocular inoculation of cell-cultured SFTSV were 100%and 83.33%(5/6),respectively on day 6 post inoculation.The mortality rates of IFNAR−/−mice with oral and ocular inoculation of SFTSV infected mouse acute serum were 100%and 66.67%(4/6),respectively on day 9 post inoculation.Conclusions:Together,our results show that SFTSV can be transmitted effectively through oral and ocular mem-brane,suggesting exposure to SFTS positive excreta may be a high-risk factor of nosocomial transmission of SFTSV in hospitals and/or families.Family members and healthcare workers should be protected properly during taking care of SFTS patients to prevent SFTSV nosocomial infection.展开更多
文摘Since throat swab specimens obtained from three adult Chinese patients were confirmed as an avian-origin influenza A （H7N9） virus by local Centers for Disease Control and Prevention （CDC） in 2013,many confirmed cases have been reported in Mainland of China.
基金This study was supported by the National Natural Sci-ence Funds of China(81971939 and 31570167)the Fundamental Research Funds for the Central Universities(2042021kf0046).
文摘Background:Severe fever with thrombocytopenia syndrome virus(SFTSV)is a tick-borne bunyavirus that could cause a severe hemorrhagic fever termed SFTS with a high fatality rate of up to 30%.Importantly,SFTSV is frequently transmitted from person-to-person and patients’blood or excreta are considered as the risk factors for transmission of SFTSV.However,the mechanism of person-to-person transmission of SFTSV is still elusive.Methods:In this study,wild-type(WT)C57BL/6 J mice and a lethal SFTSV mouse model IFNAR−/−A129 mice were utilized to evaluate whether SFTSV could be transmitted via oral or ocular routes.C57BL/6 J mice were inoculated with cell-cultured SFTSV via oral and ocular inoculation.IFNAR−/−A129 mice were inoculated with cell-cultured SFTSV or SFTSV infected mouse acute sera via oral and ocular inoculation.Results:We found that SFTSV antibody positive rates in C57BL/6 J mice were 70%(7/10)and 30%(3/10)in the oral inoculation group and ocular inoculation group,respectively on day 21 post SFTSV inoculation.The mortality rates of IFNAR−/−mice with oral and ocular inoculation of cell-cultured SFTSV were 100%and 83.33%(5/6),respectively on day 6 post inoculation.The mortality rates of IFNAR−/−mice with oral and ocular inoculation of SFTSV infected mouse acute serum were 100%and 66.67%(4/6),respectively on day 9 post inoculation.Conclusions:Together,our results show that SFTSV can be transmitted effectively through oral and ocular mem-brane,suggesting exposure to SFTS positive excreta may be a high-risk factor of nosocomial transmission of SFTSV in hospitals and/or families.Family members and healthcare workers should be protected properly during taking care of SFTS patients to prevent SFTSV nosocomial infection.
