An endophytic fungus Pestalotiopsis microspora isolated from the fruits of Manilkara zapota was cultured in potato dextrose broth media.Chromatographic separation of the EtOAc extract of the broth and mycelium led to ...An endophytic fungus Pestalotiopsis microspora isolated from the fruits of Manilkara zapota was cultured in potato dextrose broth media.Chromatographic separation of the EtOAc extract of the broth and mycelium led to the isolation of a new azaphilonoid named pitholide E(1),in addition to previously identified pitholide B(2),pitholide D(3),pestalotin(LL-P880α)(4),PC-2(5),LL-P880β(6),tyrosol(7)and 4-oxo-4H-pyran-3-acetic acid(8).An endophytic fungus P.microspora from M.zapota and the isolation of compounds 1-5,7 and 8 from P.microspora are reported here for the first time.展开更多
Three new drimane sesquiterpenoids(1–3)together with the known 2a-hydroxyisodrimeninol(4),and a new isochromone derivative(5),were obtained from the solid cultures of fungal strain Pestalotiopsis sp.M-23,an endophyti...Three new drimane sesquiterpenoids(1–3)together with the known 2a-hydroxyisodrimeninol(4),and a new isochromone derivative(5),were obtained from the solid cultures of fungal strain Pestalotiopsis sp.M-23,an endophytic fungus isolated from the leaves of Leucosceptrum canum(Labiatae).Their structures were determined by comprehensive 1D and 2D NMR,and MS analyses.The metabolites were evaluated for their antibacterial activities,and compound 3 showed weak inhibitory activity against Bacillus subtilis.展开更多
About 60% of blueberry (Vaccinium corymbosum) plantations in Argentina are located in the provinces of Entre Ríos and Buenos Aires. Alternaria tenuissima and Pestalotiopsis guepinii have been reported as pathogen...About 60% of blueberry (Vaccinium corymbosum) plantations in Argentina are located in the provinces of Entre Ríos and Buenos Aires. Alternaria tenuissima and Pestalotiopsis guepinii have been reported as pathogens of blueberry, causing leaf spots and branch cankers. The aims of this research were to estimate the prevalence of these microorganisms on leaves and fruits taken from crops located in Entre Ríos and Buenos Aires, as well as to differentiate leaf symptoms after target inoculations with each pathogen individually and in mixtures. Both fungi were present in blueberry fields from 2010 to 2013. A. tenuissima was the most prevalent pathogen, as most of the symptoms detected in the fields had been caused by this species. As a result of inoculations on cv. O’Neal, injured tissues showed symptoms before undamaged ones. Leaf symptoms caused by A. tenuissima differed from those caused by P. guepinii because of their predominant reddish color and the absence of drop-off of the central part of the lesions. When inoculated in a mixture, incubation period on leaves was intermediate between the registered for individual inoculations. The leaves showed reddishbrown spots typical of A. tenuissima and dark brown spots typical of P. guepinii, both with red margins. Blight, defoliation and canker symptoms caused in each case were undistinguishable.展开更多
Two new caryophyllene-type sesquiterpenoids, pestathenols A(1) and B(2) and one new α-furanone, pestatheranone A(6), along with five known compounds(3–5, 7 and 8) have been isolated from the crude extract of the pla...Two new caryophyllene-type sesquiterpenoids, pestathenols A(1) and B(2) and one new α-furanone, pestatheranone A(6), along with five known compounds(3–5, 7 and 8) have been isolated from the crude extract of the plant endophytic fungus Pestalotiopsis theae. Their structures were unambiguously established by extensive spectroscopic analyses. The absolute configuration of the 5,6-diol moiety in 1 was assigned using Snatzke’s method. Compounds 1 and 2 showed weak cytotoxicity against HeLa cell line.展开更多
基金Financial support from the National Research Council(Research Grant NRC 12–032)is gratefully acknowledged.
文摘An endophytic fungus Pestalotiopsis microspora isolated from the fruits of Manilkara zapota was cultured in potato dextrose broth media.Chromatographic separation of the EtOAc extract of the broth and mycelium led to the isolation of a new azaphilonoid named pitholide E(1),in addition to previously identified pitholide B(2),pitholide D(3),pestalotin(LL-P880α)(4),PC-2(5),LL-P880β(6),tyrosol(7)and 4-oxo-4H-pyran-3-acetic acid(8).An endophytic fungus P.microspora from M.zapota and the isolation of compounds 1-5,7 and 8 from P.microspora are reported here for the first time.
基金This work was supported financially by the National Natural Science Foundation of China(Nos.31525005,U1202263,31570362)the Youth Innovation Promotion association of Chinese Academy of Sciences(awarded to Shi-Hong Luo)+1 种基金the“Western Light”Program of Chinese Academy of Sciences(awarded to Shi-Hong Luo)the“Hundred Talents Program”of the Chinese Academy of Sciences(awarded to Sheng-Hong Li).
文摘Three new drimane sesquiterpenoids(1–3)together with the known 2a-hydroxyisodrimeninol(4),and a new isochromone derivative(5),were obtained from the solid cultures of fungal strain Pestalotiopsis sp.M-23,an endophytic fungus isolated from the leaves of Leucosceptrum canum(Labiatae).Their structures were determined by comprehensive 1D and 2D NMR,and MS analyses.The metabolites were evaluated for their antibacterial activities,and compound 3 showed weak inhibitory activity against Bacillus subtilis.
文摘About 60% of blueberry (Vaccinium corymbosum) plantations in Argentina are located in the provinces of Entre Ríos and Buenos Aires. Alternaria tenuissima and Pestalotiopsis guepinii have been reported as pathogens of blueberry, causing leaf spots and branch cankers. The aims of this research were to estimate the prevalence of these microorganisms on leaves and fruits taken from crops located in Entre Ríos and Buenos Aires, as well as to differentiate leaf symptoms after target inoculations with each pathogen individually and in mixtures. Both fungi were present in blueberry fields from 2010 to 2013. A. tenuissima was the most prevalent pathogen, as most of the symptoms detected in the fields had been caused by this species. As a result of inoculations on cv. O’Neal, injured tissues showed symptoms before undamaged ones. Leaf symptoms caused by A. tenuissima differed from those caused by P. guepinii because of their predominant reddish color and the absence of drop-off of the central part of the lesions. When inoculated in a mixture, incubation period on leaves was intermediate between the registered for individual inoculations. The leaves showed reddishbrown spots typical of A. tenuissima and dark brown spots typical of P. guepinii, both with red margins. Blight, defoliation and canker symptoms caused in each case were undistinguishable.
基金the National Key R&D Program of China (No. 2018YFC0311002)the National Natural Science Foundation of China (Nos. 21772228 and21977113)。
文摘Two new caryophyllene-type sesquiterpenoids, pestathenols A(1) and B(2) and one new α-furanone, pestatheranone A(6), along with five known compounds(3–5, 7 and 8) have been isolated from the crude extract of the plant endophytic fungus Pestalotiopsis theae. Their structures were unambiguously established by extensive spectroscopic analyses. The absolute configuration of the 5,6-diol moiety in 1 was assigned using Snatzke’s method. Compounds 1 and 2 showed weak cytotoxicity against HeLa cell line.
