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Establishment of High-sensitivity Rapid Fluorescence Quantitative Detection Method for Antibody against Peste des Petits Ruminants Virus
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作者 Zhao LIU Bo LIU +3 位作者 Zhida LIN Hang SUN Yu SUN Xiaohui SONG 《Agricultural Biotechnology》 2024年第5期22-27,共6页
[Objectives]This study was conducted to establish a rapid quantitative method for detecting antibody against Peste des Petits Ruminants Virus(PPR V)in sheep serum.[Methods]Soluble N protein and NH fusion protein were ... [Objectives]This study was conducted to establish a rapid quantitative method for detecting antibody against Peste des Petits Ruminants Virus(PPR V)in sheep serum.[Methods]Soluble N protein and NH fusion protein were obtained in Escherichia coli prokaryotic expression system by optimizing codons and expression conditions of E.coli.Furthermore,based on the purified soluble N protein and NH fusion protein,a high-sensitivity fluorescence immunoassay kit for detecting the antibody against PPR V was established.[Results]The method could quickly and quantitatively detect PPR V antibody in sheep serum,with high sensitivity and specificity,without any cross reaction to other related sheep pathogens.The intra-batch and inter-batch coefficients of variation were less than 10%and 15%,respectively,and the method had good repeatability.Through detection on 292 clinical serum samples,it was compared with the French IDVET competitive ELISA kit,and the coincidence rate of the two methods reached 93.84%.Compared with the serum neutralization test,the detected titer value of the high-sensitivity rapid fluorescence quantitative detection method was basically consistent with the tilter value obtained by the neutralization test on the standard positive serum(provided by the WOAH Brucellosis Reference Laboratory of France).[Conclusions]This method can realize rapid quantitative detection of PPR V antibody on site,and has high practical value and popularization value. 展开更多
关键词 peste des petits ruminants N protein NH fusion protein Soluble expression and purification Rapid quantitative detection
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Establishment of Double-antigen Sandwich Time-resolved Fluorescence Immunoassay for Detection of Pest des Petits Ruminants Virus
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作者 Binglei CAO Zhongyuan GE +3 位作者 Qi YANG Hang SUN Yu SUN Xiaohui SONG 《Agricultural Biotechnology》 2024年第4期21-27,共7页
[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PP... [Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value. 展开更多
关键词 peste des petits ruminants N active protein NH fusion protein Soluble expression and purification Time-resolved fluorescence immunoassay
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Monitoring and Tracking on Immune Antibody of Sheep Peste des Petits Ruminants 被引量:1
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作者 Lv Yanqiu Huang Dongfeng +8 位作者 Wang Meili Wang Jinxia Wang Yuewei Qiu Hailian Gao Xiaobo Zhang Yichi Kang Xiaojie Shan Lihua Xue Yong 《Animal Husbandry and Feed Science》 CAS 2017年第2期96-97,共2页
Peste ties petits ruminants is a kind of acute eontagious disease infecting goats anti sheep. In this study, antibtly monitoring and tracking of healthy goat and sheep immunized by peste des petits ruminants vaccine i... Peste ties petits ruminants is a kind of acute eontagious disease infecting goats anti sheep. In this study, antibtly monitoring and tracking of healthy goat and sheep immunized by peste des petits ruminants vaccine in Changping District of Beijing City were conducted, aiming at providing a reference for the devel- opment of effective immunization procedures. 展开更多
关键词 MONITORING Immune antibody Sheep peste des petits ruminants
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Seroprevalence and Associated Risk Factors of Peste des Petits Ruminants among Sheep and Goats in Kassala State, Sudan
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作者 Fatima A. Saeed Sana A. Abdel-Aziz Mohammed M. Gumaa 《Open Journal of Animal Sciences》 2018年第4期381-395,共15页
Peste des petits ruminant (PPR) is a contagious disease of small ruminants caused by a virus that belongs to the genus Morbillivirus of the family Paramyxoviridae. This study aimed to determine the seroprevalence of P... Peste des petits ruminant (PPR) is a contagious disease of small ruminants caused by a virus that belongs to the genus Morbillivirus of the family Paramyxoviridae. This study aimed to determine the seroprevalence of PPR disease in sheep and goats and its associated risk factors in Kassala State, Eastern Sudan. Across sectional study was conducted during the period from 30th August to 25th November 2015. The study was carried out using a structured questionnaire survey and a total of 918 blood samples were collected from apparently healthy unvaccinated sheep and goats in different localities in State of Kassala. A total of 546 sheep and 372 goats were tested for specific antibodies to nucleoprotein (NP) by competitive enzyme linked immunosorbent assay (cELISA). The apparent overall prevalence of PPR antibodies in Kassala was 58.2% while the true prevalence was calculated to be 61.3%. The apparent prevalence in sheep and goats was 68.1% and 43.5% respectively. Univariate analysis showed that the risk factors had significant associations with a cELISA positive status: locality, species, age, breed, husbandry system, housing mode, animals movement (p = 0.000) and animals sharing pasture and water (p = 0.003), while sex and newly introduced animals were not significant risk factors (p = 0.771) (p = 0.050) respectively. Factors found that significantly associated (p < 0.05) with increased odds of being cELISA positive in multivariate analysis were localities, species, age and newly introduced animals. The prevalence differed between localities and was the highest in the River Atbara (84.0%) locality, whereas it was lowest in Delta North (29.0%). No significant difference was observed among the sexes. However, the prevalence differed in different age groups and was 52.25% in animals of less than six months old;49.3% were between seven months and two years old and 65.5% were above two years old. In different husbandry systems, the prevalence was 47.9%, 73.0% and 49.2% in intensive, open grazing and pastoral systems respectively. Housing type effects were also observed;the highest prevalence was in animals housed in metal fence (83.3%). The movement pattern showed significant effect, where the prevalence was the highest (81.3%) in animals that move inter-states/inter-localities. It is concluded that the disease is endemic in Kassala State, high prevalent in sheep and goats, posing a threat to animal exportation, and may have a serious economic influence. Owners and herders should compulsorily vaccinate their animals yearly and animals should be investigated periodically for implementation of crucial eradication program. 展开更多
关键词 peste des petits ruminantS SERO-PREVALENCE Risk Factors Kassala STATE SUDAN
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Sero-Prevalence and Risk Factors of Diffusion of Peste des Petits Ruminants in Cameroon
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作者 Severin Loul Abel Wade Alexandre Michel Njan Nlôga 《Open Journal of Veterinary Medicine》 2020年第7期103-115,共13页
The present study was carried out between April 2015 and January 2016 to estimate the sero-prevalence and identify the risk factors of the peste des petits ruminants (PPR) in Cameroon. A total of 269 herds randomly sa... The present study was carried out between April 2015 and January 2016 to estimate the sero-prevalence and identify the risk factors of the peste des petits ruminants (PPR) in Cameroon. A total of 269 herds randomly sampled across the country have been studied and 1622 samples of serum have been levied on the sheep and goat. The c-ELISA has been studied in order to detect the presence of antibodies in small ruminants like an indicator of exposition to PPRV. The results revealed the circulation of PPRV in the country with a total sero-prevalence of 39% [95%CI;37 - 41] and a sero-prevalence of 63.2% [95%CI;57.2 - 69.2] at the herd level. Sero-prevalence was variable in the ten regions ranging from 7% [95% CI;6.2 - 8.4] to 73% [95% CI;62 - 84] with the northern zone (Adamawa, North and Far-North) having 52.3% [95% CI;37 - 60] and southern zone (including the remaining seven regions) recording 29% [95% CI;11 - 57]. Similarly, it was higher in animals found in urban/peri-urban areas than in rural areas with prevalence ratio of 2.9 [95% CI 2.54 - 3.4;p < 0.001] <em>i.e. </em>3 times more, 1.6 [95% CI 1.36 - 1.90;p < 0.001] <em>i.e.</em> 1.6 times more, and 5.02 [95% CI 3.91 - 6.85;p < 0.001] <em>i.e.</em> 5 times more at national level, in the northern zone and in the southern area, respectively. Five risk factors have been identified: the breeding environment, introduction of new animals into the herds, gathering of animals for pasture and watering, wandering and transhumance. The breeding area appeared to be the most important risk factor associated with disease exposure. The control measures for the eradication of this disease must take into account the epidemiological situation, the breeding environment, animal transhumance and breeding system. 展开更多
关键词 Small ruminants SERO-PREVALENCE Risk Factors peste des petits ruminants Cameroon
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2021—2023年云南临沧市小反刍兽疫病原学和免疫抗体检测分析
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作者 李亚琴 蔡高萍 +2 位作者 谭东 杨依波 杨小芳 《养殖与饲料》 2024年第5期27-30,共4页
[目的]为全面掌握临沧市小反刍兽疫免疫效果,科学评估其感染状况。[方法]随机采集2021—2023年来自规模场和散养户的羊血清4 524份、羊眼鼻棉拭子1 000份,采用阻断ELISA方法进行小反刍兽疫抗体检测,病毒核酸荧光RT-PCR进行病原学检测。... [目的]为全面掌握临沧市小反刍兽疫免疫效果,科学评估其感染状况。[方法]随机采集2021—2023年来自规模场和散养户的羊血清4 524份、羊眼鼻棉拭子1 000份,采用阻断ELISA方法进行小反刍兽疫抗体检测,病毒核酸荧光RT-PCR进行病原学检测。[结果]小反刍兽疫免疫抗体合格率,2022年比2021年提高了7.44百分点,2023年比2022年提高了3.11百分点,病原学未检测出小反刍兽疫病毒核酸阳性样品。[结论]临沧市以“人病兽防、关口前移”各项防控措施为抓手,小反刍兽疫的免疫效果总体较好。 展开更多
关键词 小反刍兽疫 抗体检测 病原学检测 疫苗免疫 小反刍兽疫病毒 荧光RT-PCR
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2021—2023年贵州省凯里市牛羊重要疫病免疫效果调查分析
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作者 李心琼 袁晓娟 《中国乳业》 2024年第7期78-82,共5页
[目的]为做好牛羊疫病防控专项工作,科学评估牛羊口蹄疫和小反刍兽疫疫苗免疫效果。[方法]用ELISA方法对2021—2023年采集的凯里市牛羊血清进行免疫抗体检测。[结果]2021—2023年,规模场牛群O型口蹄疫免疫平均抗体合格率87.69%~89.31%,... [目的]为做好牛羊疫病防控专项工作,科学评估牛羊口蹄疫和小反刍兽疫疫苗免疫效果。[方法]用ELISA方法对2021—2023年采集的凯里市牛羊血清进行免疫抗体检测。[结果]2021—2023年,规模场牛群O型口蹄疫免疫平均抗体合格率87.69%~89.31%,散养户牛群平均合格率65.91%~67.20%。规模场牛群A型口蹄疫免疫平均抗体合格率71.15%~72.07%,散养户牛群平均合格率55.91%~57.60%。规模场羊群O型口蹄疫免疫平均抗体合格率82.94%~85.56%,散养户平均合格率70.53%~72.27%。羊A型口蹄疫免疫平均抗体合格率65.88%~67.22%,散养户平均合格率53.68%~55.91%。规模场羊群小反刍兽疫免疫抗体合格率80.00%~81.11%,散养羊群平均抗体合格率71.05%~73.18%。[结论]贵州省凯里市牛羊O型口蹄疫免疫效果较好,牛羊A型口蹄疫免疫效果相对较差,羊小反刍兽疫免疫效果很好,但牛羊O/A型口蹄疫免疫效果散养户比规模场差,规模场羊群小反刍兽疫免疫抗体合格率比散养户高。需加强规模场、散养户牛羊A型口蹄疫免疫和检测,同时加强散养户羊群小反刍兽疫免疫与效果评估,确保不发生重大动物疫情。 展开更多
关键词 凯里市 口蹄疫 小反刍兽疫 免疫
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小反刍兽疫疫苗免疫效果监测
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作者 刘颖 章天霞 《福建畜牧兽医》 2024年第3期12-14,共3页
为监测小反刍兽疫-山羊痘二联活疫苗对小反刍兽疫的免疫效果,结合桐城市“先打后补”项目的实施,分别抽取3个规模养殖场免疫注射后7 d、35 d羊血清样品180份,用竞争ELISA法进行检测。结果表明,小反刍兽疫-山羊痘二联活疫苗在免疫注射后... 为监测小反刍兽疫-山羊痘二联活疫苗对小反刍兽疫的免疫效果,结合桐城市“先打后补”项目的实施,分别抽取3个规模养殖场免疫注射后7 d、35 d羊血清样品180份,用竞争ELISA法进行检测。结果表明,小反刍兽疫-山羊痘二联活疫苗在免疫注射后7 d产生免疫抗体;35 d抗体滴度高,抗体阳性率高达92.2%,符合国家规定的群体免疫抗体合格率在70%以上要求,免疫效果确实,可有效阻遏小反刍兽疫的发生与流行。 展开更多
关键词 小反刍兽疫 疫苗 免疫抗体 合格率
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2021—2023年凯里市肉羊主要疫病监测数据分析与防控建议
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作者 袁晓娟 刘铭英 《畜禽业》 2024年第5期81-84,共4页
为掌握凯里市肉羊小反刍兽疫、口蹄疫免疫效果及布鲁氏菌病流行情况,对2021—2023年凯里市58个场(户)采集山羊血清样品共540份,应用ELISA试验、虎红平板凝集试验对羊口蹄疫(O型)、小反刍兽疫、布鲁氏菌病进行实验室检测。结果表明:山羊... 为掌握凯里市肉羊小反刍兽疫、口蹄疫免疫效果及布鲁氏菌病流行情况,对2021—2023年凯里市58个场(户)采集山羊血清样品共540份,应用ELISA试验、虎红平板凝集试验对羊口蹄疫(O型)、小反刍兽疫、布鲁氏菌病进行实验室检测。结果表明:山羊口蹄疫(O型)免疫抗体合格率为83.07%;小反刍兽疫免疫抗体合格率为77.04%;应用虎红平板凝集试验诊断布鲁氏菌病,阳性率为0%。羊口蹄疫免疫防制工作较为有效,平均抗体合格率保持在80%以上;连续3年未检出布鲁氏菌病阳性;小反刍兽疫平均抗体合格率虽达到农业部标准,但是散养户的免疫抗体合格率为68.75%;有2个乡镇小反刍兽疫抗体合格率低于70%,应加强免疫和检测工作,提高警惕。 展开更多
关键词 肉羊 口蹄疫 小反刍兽疫 布鲁氏菌病 检测
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Reverse Genetics for Peste des Petits Ruminants Virus: Current Status and Lessons to Learn from Other Non-segmented Negative-Sense RNA Viruses 被引量:4
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作者 Alfred Niyokwishimira Yongxi Dou +2 位作者 Bang Qian Prajapati Meera Zhidong Zhang 《Virologica Sinica》 SCIE CAS CSCD 2018年第6期472-483,共12页
Peste des petits ruminants(PPR) is a highly contagious transboundary animal disease with a severe socio-economic impact on the livestock industry, particularly in poor countries where it is endemic. Full understanding... Peste des petits ruminants(PPR) is a highly contagious transboundary animal disease with a severe socio-economic impact on the livestock industry, particularly in poor countries where it is endemic. Full understanding of PPR virus(PPRV)pathobiology and molecular biology is critical for effective control and eradication of the disease. To achieve these goals,establishment of stable reverse genetics systems for PPRV would play a key role. Unfortunately, this powerful technology remains less accessible and poorly documented for PPRV. In this review, we discussed the current status of PPRV reverse genetics as well as the recent innovations and advances in the reverse genetics of other non-segmented negative-sense RNA viruses that could be applicable to PPRV. These strategies may contribute to the improvement of existing techniques and/or the development of new reverse genetics systems for PPRV. 展开更多
关键词 peste des petits ruminants(ppr) pprV Reverse GENETICS Non-segmented negative-sense RNA virus
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贵州省影响羊口蹄疫和小反刍免疫效果分析 被引量:3
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作者 王娜 张天天 +3 位作者 程振涛 文明 王开功 周碧君 《中国动物传染病学报》 CAS 北大核心 2023年第6期127-132,共6页
为研究贵州省不同地区、养殖方式、海拔和温度对羊A、O、Asia I型FMD和PPR免疫抗体阳性率的影响。采用酶联免疫吸附实验对2015~2020年本实验室收集的贵州省9个市(州)515个养殖场的羊血清(共计8869份)进行上述疫病的免疫抗体检测,并对检... 为研究贵州省不同地区、养殖方式、海拔和温度对羊A、O、Asia I型FMD和PPR免疫抗体阳性率的影响。采用酶联免疫吸附实验对2015~2020年本实验室收集的贵州省9个市(州)515个养殖场的羊血清(共计8869份)进行上述疫病的免疫抗体检测,并对检测结果使用易侕统计软件和R语言进行分析。统计结果表明:羊A、O、Asia I型FMD和PPR的免疫抗体平均阳性率分别为42.57%、87.99%、73.64%和64.45%。R语言分析结果表明:羊A、O、Asia I型FMD和PPR免疫抗体阳性率在贵州省的不同地区中差异不显著(P>0.05);不同养殖方式对羊A、O、Asia I型FMD和PPR的免疫效果影响差异不显著(P>0.05);不同海拔对羊A、O、Asia I型FMD和PPR的免疫效果影响差异不显著(P>0.05);不同温度对羊A、O型FMD的免疫效果影响差异极显著(P<0.01)。结果提示,贵州省羊O、Asia I型FMD免疫抗体阳性率达到国家农业部动物疫病监测合格要求,羊A型FMD与PPR免疫抗体阳性率未达到国家农业部动物疫病监测合格要求;温度对羊A、O型FMD的免疫效果影响较大。本研究为贵州省羊A、O、Asia I型FMD疫苗合理免疫程序的制定提供一定的理论依据。 展开更多
关键词 口蹄疫 小反刍 免疫效果
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小反刍兽疫和山羊痘二联灭活疫苗的制备及其免疫原性 被引量:1
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作者 应普红 刘艳芬 +1 位作者 陈绍红 刘铀 《中国草食动物科学》 CAS 2023年第3期14-20,共7页
为了研制小反刍兽疫(Peste des petits ruminants,PPR)和山羊痘(Goat pox,GTP)二联油剂灭活疫苗,以Vero细胞增殖小反刍兽疫病毒Nigeria75/1 Clone 9株和山羊痘病毒CVCC AV41株,通过细胞病变观察、间接免疫荧光试验和荧光定量PCR确定接... 为了研制小反刍兽疫(Peste des petits ruminants,PPR)和山羊痘(Goat pox,GTP)二联油剂灭活疫苗,以Vero细胞增殖小反刍兽疫病毒Nigeria75/1 Clone 9株和山羊痘病毒CVCC AV41株,通过细胞病变观察、间接免疫荧光试验和荧光定量PCR确定接种方式、接毒剂量和收毒时间对病毒增殖的影响。采用细胞病变观察、SDS-PAGE和Western-blot比较甲醛、二乙烯亚胺和β-丙内酯灭活病毒的效果。随后在灭活的病毒悬液中加入等体积的油佐剂进行乳化,制备PPRV-GTPV二联油剂灭活疫苗,并参照《中国兽药典》检测疫苗理化性状。为评价疫苗的免疫原性,将24只2月龄临床健康的雷州山羊随机分为3组(每组8只),分别肌肉注射生理盐水、PPRV-GTPV二联灭活疫苗、商品化PPRV-GTPV弱毒疫苗。免疫后14 d和28 d分别检测试验山羊血清PPRV和GTPV中和抗体。结果表明,PPRV和GTPV以100 TCID50/mL的剂量共感染Vero细胞84 h后的病毒滴度较高。用终浓度为0.025%的β-丙内酯4℃处理24 h,能有效灭活PPRV和GTPV;制备的灭活疫苗符合《中国兽药典》相关要求。PPRV和GTPV二联灭活疫苗免疫14 d后,山羊血清PPRV和GTPV中和抗体水平与商品化弱毒疫苗相仿;免疫28 d后,山羊血清PPRV中和抗体显著高于商品化弱毒疫苗,GTPV抗体呈上升趋势,但显著低于商品化弱毒疫苗。试验山羊均未见明显不良反应。综上说明,PPRV-GTPV二联灭活疫苗安全、有效,有望在小反刍兽疫和山羊痘的防制中发挥重要作用。 展开更多
关键词 小反刍兽疫 山羊痘 二联灭活疫苗 免疫原性
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口蹄疫、小反刍兽疫、羊痘三种疫苗不同免疫方案的免疫效果分析研究 被引量:3
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作者 李吉鹏 周兴玉 李宇光 《畜牧兽医杂志》 2023年第6期129-132,共4页
口蹄疫、小反刍兽疫两种疫苗是国家规定羊的强制免疫疫苗,羊痘疫苗是金昌市因病设防的免疫疫苗。实际工作中,因上述三种疫苗免疫程序繁琐,劳动强度大,存在羊群免疫密度和抗体保护率低等问题,免疫效果不理想。通过研究口蹄疫、小反刍兽... 口蹄疫、小反刍兽疫两种疫苗是国家规定羊的强制免疫疫苗,羊痘疫苗是金昌市因病设防的免疫疫苗。实际工作中,因上述三种疫苗免疫程序繁琐,劳动强度大,存在羊群免疫密度和抗体保护率低等问题,免疫效果不理想。通过研究口蹄疫、小反刍兽疫、羊痘三种疫苗不同免疫方案,分析免疫效果,发现“三苗三针三免”“三苗三针两免”“三苗三针一免”免疫安全有保障、抗体合格率高,“三苗两针一免”免疫安全无保障、抗体合格率不达标。 展开更多
关键词 口蹄疫 小反刍兽疫 羊痘 疫苗 免疫方案 副反应 合格率
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小反刍兽疫病毒的研究进展 被引量:19
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作者 李林杰 谢军 +2 位作者 徐文凯 马晓霞 柏家林 《黑龙江畜牧兽医》 CAS 北大核心 2018年第1期66-70,252,共6页
小反刍兽疫(peste des petits ruminants,PPR)是由一种副黏病毒科麻疹病毒属病毒引起的主要感染山羊、绵羊的急性高治病性传染病,但牛感染后临床症状不明显。世界动物卫生组织(OIE)将其列为A类疫病,感染率和死亡率高达90%。由于绵羊和... 小反刍兽疫(peste des petits ruminants,PPR)是由一种副黏病毒科麻疹病毒属病毒引起的主要感染山羊、绵羊的急性高治病性传染病,但牛感染后临床症状不明显。世界动物卫生组织(OIE)将其列为A类疫病,感染率和死亡率高达90%。由于绵羊和山羊是非洲和西亚贫困人民的重要生产资料,PPR对食品安全和摆脱贫困构成巨大威胁。小反刍兽疫病毒(PPRV)和牛瘟病毒(RPV)与麻疹病毒(MV)联系紧密。通过大规模接种疫苗牛瘟已被彻底消除。虽然可以利用弱毒疫苗免疫机体预防PPRV感染,但由于其在亚热带气候中的热不稳定性、使用时所需剂量的不确定性及接种范围的不足导致该病仍未得到有效控制。另外已有证据表明,在疫苗与不同毒株之间存在很少的交叉中和,使得目前流通的PPRV疫苗的保护功效被质疑。文章简要介绍了目前全世界对PPRV的高关注度及PPRV的生物学特性、发病机理等。 展开更多
关键词 小反刍兽疫(ppr) 小反刍兽疫病毒(pprV) 蛋白 结构 病毒复制
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全球小反刍兽疫流行趋势 被引量:17
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作者 朱迪国 宋建德 +1 位作者 袁丽萍 魏荣 《中国动物检疫》 CAS 2014年第6期14-16,共3页
2013年以来,全球共43个国家报告发生小反刍兽疫(PPR)。其中,疫情仍然集中在亚洲和非洲。本文介绍了全球小反刍兽疫的发生情况,尤其是我国周边国家的疫情情况,分析了全球小反刍兽疫的扩散趋势。
关键词 小反刍兽疫 流行 趋势
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小反刍兽疫病毒的分子生物学特性及其在全球的流行 被引量:21
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作者 罗静 何宏轩 《河北师范大学学报(自然科学版)》 CAS 北大核心 2009年第4期543-550,共8页
小反刍兽疫由副粘病毒科麻疹病毒属小反刍兽疫病毒引起,经直接或间接接触传染,主要感染山羊、绵羊等小反刍动物,严重影响畜牧业,造成巨大经济损失.1942年首次发现于西非科特迪瓦,逐渐扩散至非洲及亚洲大部,涉及四十余国家.2007年7月首... 小反刍兽疫由副粘病毒科麻疹病毒属小反刍兽疫病毒引起,经直接或间接接触传染,主要感染山羊、绵羊等小反刍动物,严重影响畜牧业,造成巨大经济损失.1942年首次发现于西非科特迪瓦,逐渐扩散至非洲及亚洲大部,涉及四十余国家.2007年7月首次传入中国西藏阿里地区,并于2008年6月再度爆发,且呈由边境逐渐向内传播的趋势.该地区有多种珍贵濒危野生反刍动物,是小反刍兽疫病毒的潜在宿主,它们随季节迁徙及夏季产仔的习性更增加其受小反刍兽疫感染并传播小反刍兽疫的可能.因此,对小反刍兽疫病毒的生物学特征、流行特点和防控措施进行综述,必将有利于正确评估小反刍兽疫对野生有蹄类动物的威胁并采取合适的措施预防与控制小反刍兽疫疫情. 展开更多
关键词 小反刍兽疫 分子生物学特性 流行 野生有蹄类动物
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小反刍兽疫活疫苗的安全性评价试验 被引量:5
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作者 薛青红 印春生 +1 位作者 李宁 支海兵 《中国兽药杂志》 2010年第9期4-6,共3页
2007年小反刍兽疫(PPR)在我国西藏首次暴发,在西藏和新疆部分地区使用PPR Nigeria 75/1疫苗株制造的疫苗进行免疫接种。为明确疫苗的安全性,中国兽医药品监察所国家牛瘟参考实验室对其安全性能进行了系统评价。健康易感山羊、绵羊及怀... 2007年小反刍兽疫(PPR)在我国西藏首次暴发,在西藏和新疆部分地区使用PPR Nigeria 75/1疫苗株制造的疫苗进行免疫接种。为明确疫苗的安全性,中国兽医药品监察所国家牛瘟参考实验室对其安全性能进行了系统评价。健康易感山羊、绵羊及怀孕山羊、怀孕绵羊按不同剂量接种疫苗后,均未观察到异常临床反应;怀孕母羊所产羔羊数量与对照组无明显差异。疫苗对小白鼠、豚鼠的非特异性安全试验表明,所有接种动物均健活。结果表明该疫苗安全性良好,可在田间大规模使用。 展开更多
关键词 小反刍兽疫 疫苗 安全
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小反刍兽疫免疫荧光诊断技术研究 被引量:1
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作者 蒋梅 赵文华 杨仕标 《黑龙江畜牧兽医》 CAS 北大核心 2012年第4期102-105,174,共5页
为了建立小反刍兽疫(PPR)荧光抗体诊断方法,研究选用小反刍兽疫病毒(PPRV)Nigeria75/1减毒疫苗株制备抗原,免疫试验山羊,制备小反刍兽疫高免血清,用硫酸铵盐析法粗提免疫球蛋白(IgG),再应用提取的IgG制备异硫氰酸荧光素标记抗体。结果表... 为了建立小反刍兽疫(PPR)荧光抗体诊断方法,研究选用小反刍兽疫病毒(PPRV)Nigeria75/1减毒疫苗株制备抗原,免疫试验山羊,制备小反刍兽疫高免血清,用硫酸铵盐析法粗提免疫球蛋白(IgG),再应用提取的IgG制备异硫氰酸荧光素标记抗体。结果表明:所制备的荧光抗体对试验用Vero细胞组织抗原和犬瘟热病毒(CDV)抗原无反应性,对小反刍兽疫病毒感染细胞的检出敏感性为1×106稀释度。 展开更多
关键词 小反刍兽疫(ppr) 诊断方法 荧光抗体
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规模羊场疫苗联合免疫规程优化研究 被引量:8
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作者 常攀峰 张莉敏 +1 位作者 党岩 马志宏 《畜牧兽医杂志》 2022年第4期69-71,共3页
目的对规模羊场常用疫苗,即羊小反刍兽疫疫苗、口蹄疫疫苗和羊痘疫苗联合免疫程序进行优化设计。方法分别设置口蹄疫疫苗组、小反刍兽疫疫苗组和山羊痘疫苗组、两两免疫组和三种疫苗同时免疫组来分别免疫绵羊,测定各试验组免疫后7 d、1... 目的对规模羊场常用疫苗,即羊小反刍兽疫疫苗、口蹄疫疫苗和羊痘疫苗联合免疫程序进行优化设计。方法分别设置口蹄疫疫苗组、小反刍兽疫疫苗组和山羊痘疫苗组、两两免疫组和三种疫苗同时免疫组来分别免疫绵羊,测定各试验组免疫后7 d、14 d、21 d、28 d、60 d、90 d、150 d的实验羊免疫血清抗体产生情况及抗体存续时间。结果:小反刍兽疫疫苗免疫效果最优为单独免疫组,口蹄疫疫苗免疫效果最优为口蹄疫、小反刍兽疫联合免疫组,羊痘疫苗免疫效果最优为羊痘单独免疫组。结论可采用口蹄疫、小反刍兽疫联合免疫,羊痘单独免疫的免疫模式,既可以减少免疫副反应又能提高免疫效果,同时降低了免疫接种的劳动强度。 展开更多
关键词 口蹄疫疫苗 小反刍兽疫活疫苗 山羊痘活疫苗 联合免疫
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小反刍兽疫诊断技术的研究进展 被引量:3
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作者 吴宣 张永宁 +2 位作者 关泽英 毛光琼 王泽洲 《四川畜牧兽医》 2014年第6期30-32,共3页
小反刍兽疫是由小反刍兽疫病毒引起的一种重大烈性传染病,主要发生于山羊和绵羊等小反刍兽。该病无特效治疗方法,主要通过早期诊断和疫苗免疫的方式控制。本文主要就小反刍兽疫诊断技术的研究进展作一综述。
关键词 小反刍兽疫 流行病学 诊断技术
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