Objective:Pseudostellaria heterophylla has been paid more attention in recent years,mainly as a medicine food homology plant.The content determination of P.heterophylla is not specified in the Chinese Pharmacopoeia(ve...Objective:Pseudostellaria heterophylla has been paid more attention in recent years,mainly as a medicine food homology plant.The content determination of P.heterophylla is not specified in the Chinese Pharmacopoeia(version 2020).The environmental conditions in different production areas could exert an influence on the quality of P.heterophylla.The purpose of this study is to discriminate P.heterophylla collected from different geographical origins of China.Methods:In this study,the content of polysaccharide in 28 batches of P.heterophylla was determined using phenol–sulfuric acid.HPLC fingerprints were established under optimised HPLC-PDA methods.Subsequently,the similarity analysis(SA)and the quantification of heterophyllin B were analyzed.The metabolites of P.heterophylla were identified and evaluated using UHPLC-Q Exactive HF orbitrap MS system.Principal component analysis(PCA),partial least squares discriminant analysis(PLS-DA),hierarchical cluster analysis(HCA)and orthogonal PLS-DA(OPLS-DA)were performed based on all peak areas.Results:The polysaccharide content in Guizhou and Jiangsu was higher than that of other production areas,which varied significant from different origins.While the content of heterophyllin B in Anhui and Jiangsu was high.The correlation coefficients of HPLC fingerprints for 28 batches samples ranged from 0.877 to 0.990,and the characteristic map can be used to identify and evaluate the quality of P.heterophylla.The samples from Fujian,Guizhou,Jiangsu provinces can be relatively separated using multivariate statistical analysis including PCA,PLS-DA,HCA,OPLS-DA,indicating that their metabolic compositions were significantly different.Ultimately,a total of 15 metabolites which were filtrated by a VIPvalue>1 and a P-value<0.05 associated with the separation of different origins were identified.Conclusion:HPLC fingerprint was established to evaluate the quality and authenticity of P.heterophylla.The present work showed that the difference of geographic distributions had an influence on the internal chemical compositions.A sensitive and rapid untargeted metabolomics approach by UHPLC-Q Exactive HF orbitrap MS was utilized to evaluate P.heterophylla from different origins in China for the first time.Overall,this study provides insights to metabolomics of P.heterophylla and supplies important reference values for the development of functional foods.展开更多
应用RAPD标记方法分析了15个太子参〔Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm.〕种源间的遗传多样性和亲缘关系。10条随机引物共扩增出65条带,其中多态性条带37条,多态性条带百分率达56.9%。用聚类分析方法可将15个太...应用RAPD标记方法分析了15个太子参〔Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm.〕种源间的遗传多样性和亲缘关系。10条随机引物共扩增出65条带,其中多态性条带37条,多态性条带百分率达56.9%。用聚类分析方法可将15个太子参种源分为4类;地理分布越近,太子参种源间的遗传差异越小。来源于安徽宣城的太子参种源遗传变异明显,辽宁凤城的野生太子参与山东地区的太子参栽培种源间的亲缘关系较近,与江苏各地太子参种源的亲缘关系则较远,这些种源均可作为育种材料。自然环境,尤其是生态环境的变化,对太子参的遗传变异有一定的影响。展开更多
基金supported by the National Key R&D Program of China(No.2019YFC1710604)the National Key Research and Development Program(No.2020YFA0907903)+2 种基金the key Project at Central Government Level(No.2060302)Tianjin Science and Technology Plan Project(No.19YFZCSY00170)the National Natural Science Foundation of China(No.81872956)。
文摘Objective:Pseudostellaria heterophylla has been paid more attention in recent years,mainly as a medicine food homology plant.The content determination of P.heterophylla is not specified in the Chinese Pharmacopoeia(version 2020).The environmental conditions in different production areas could exert an influence on the quality of P.heterophylla.The purpose of this study is to discriminate P.heterophylla collected from different geographical origins of China.Methods:In this study,the content of polysaccharide in 28 batches of P.heterophylla was determined using phenol–sulfuric acid.HPLC fingerprints were established under optimised HPLC-PDA methods.Subsequently,the similarity analysis(SA)and the quantification of heterophyllin B were analyzed.The metabolites of P.heterophylla were identified and evaluated using UHPLC-Q Exactive HF orbitrap MS system.Principal component analysis(PCA),partial least squares discriminant analysis(PLS-DA),hierarchical cluster analysis(HCA)and orthogonal PLS-DA(OPLS-DA)were performed based on all peak areas.Results:The polysaccharide content in Guizhou and Jiangsu was higher than that of other production areas,which varied significant from different origins.While the content of heterophyllin B in Anhui and Jiangsu was high.The correlation coefficients of HPLC fingerprints for 28 batches samples ranged from 0.877 to 0.990,and the characteristic map can be used to identify and evaluate the quality of P.heterophylla.The samples from Fujian,Guizhou,Jiangsu provinces can be relatively separated using multivariate statistical analysis including PCA,PLS-DA,HCA,OPLS-DA,indicating that their metabolic compositions were significantly different.Ultimately,a total of 15 metabolites which were filtrated by a VIPvalue>1 and a P-value<0.05 associated with the separation of different origins were identified.Conclusion:HPLC fingerprint was established to evaluate the quality and authenticity of P.heterophylla.The present work showed that the difference of geographic distributions had an influence on the internal chemical compositions.A sensitive and rapid untargeted metabolomics approach by UHPLC-Q Exactive HF orbitrap MS was utilized to evaluate P.heterophylla from different origins in China for the first time.Overall,this study provides insights to metabolomics of P.heterophylla and supplies important reference values for the development of functional foods.
文摘应用RAPD标记方法分析了15个太子参〔Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm.〕种源间的遗传多样性和亲缘关系。10条随机引物共扩增出65条带,其中多态性条带37条,多态性条带百分率达56.9%。用聚类分析方法可将15个太子参种源分为4类;地理分布越近,太子参种源间的遗传差异越小。来源于安徽宣城的太子参种源遗传变异明显,辽宁凤城的野生太子参与山东地区的太子参栽培种源间的亲缘关系较近,与江苏各地太子参种源的亲缘关系则较远,这些种源均可作为育种材料。自然环境,尤其是生态环境的变化,对太子参的遗传变异有一定的影响。
