Effects of bioremediation on microbial communities in soils contaminated with petroleum hydrocarbons are a scientific problem to be solved. Changes in dominate microbial species and the total amount of microorganisms ...Effects of bioremediation on microbial communities in soils contaminated with petroleum hydrocarbons are a scientific problem to be solved. Changes in dominate microbial species and the total amount of microorganisms including bacteria and fungi in rhizospheric soils after bioremediation were thus evaluated using field bioremediation experiments. The results showed that there were changed dominant microorganisms including 11 bacterial strains which are mostly Gram positive bacteria and 6 fungal species which were identified. The total amount of microorganisms including bacteria and fungi increased after bioremediation of microbial agents combined with planting maize. On the contrary, fungi in rhizospheric soils were inhibited by adding microbial agents combined with planting soybean.展开更多
The bioremediation potential of bacteria indigenous to soils of the Yellow River Delta in China was evaluated as a treatment option for soil remediation. Petroleum hydrocarbon degraders were isolated from contaminated...The bioremediation potential of bacteria indigenous to soils of the Yellow River Delta in China was evaluated as a treatment option for soil remediation. Petroleum hydrocarbon degraders were isolated from contaminated soil samples from the Yellow River Delta. Four microbial communities and eight isolates were obtained. The optimal temperature, salinity, pH, and the ratios of C, N, and P (C:N:P) for the maximum biodegradation of diesel oil, crude oil, n-alkanes, and polyaromatic hydrocarbons by indigenous bacteria were determined, and the kinetics changes in microbial communities were monitored. In general, the mixed microbial consortia demonstrated wider catabolic versatility and faster overall rate of hydrocarbon degradation than individual isolates. Our experimental results demonstrated the feasibility of biodegradation of petroleum hydrocarbon by indigenous bacteria for soil remediation in the Yellow River Delta.展开更多
Diversity in bacterial communities was investigated along a petroleum hydrocarbon content gradient(0-0.4043 g/g)in surface(5-10 cm)and subsurface(35-40 cm)petroleum-contaminated soil samples from the Dagang Oilfield,C...Diversity in bacterial communities was investigated along a petroleum hydrocarbon content gradient(0-0.4043 g/g)in surface(5-10 cm)and subsurface(35-40 cm)petroleum-contaminated soil samples from the Dagang Oilfield,China.Using 16S rRNA Illumina high-throughput sequencing technology and several statistical methods,the bacterial diversity of the soil was studied.Subsequently,the environmental parameters were measured to analyze its relationship with the community variation.Nonmetric multidimensional scaling and analysis of similarities indicated a significant difference in the structure of the bacterial community between the nonpetroleum-contaminated surface and subsurface soils,but no differences were observed in different depths of petroleum-contaminated soil.Meanwhile,many significant correlations were obtained between diversity in soil bacterial community and physicochemical properties.Total petroleum hydrocarbon,total organic carbon,and total nitrogen were the three important factors that had the greatest impacts on the bacterial community distribution in the long-term petroleum-contaminated soils.Our research has provided references for the bacterial community distribution along a petroleum gradient in both surface and subsurface petroleum-contaminated soils of oilfield areas.展开更多
Although petroleum is an important source of energy and an economic driver of growth,it is also a major soil pollutant that has destroyed large swathes of vegetation and forest cover.Therefore,it is vital to develop a...Although petroleum is an important source of energy and an economic driver of growth,it is also a major soil pollutant that has destroyed large swathes of vegetation and forest cover.Therefore,it is vital to develop affordable and efficient methods for the bioremediation of petroleum-contaminated forest soils to restore vegetation and improve tree survival rates.In this study,bioremediation experiments were performed in an electrically heated thermostatic reactor to test the effects of organic matter additives,surfactants,and oxygen providers of nine hydrocarbon-degrading fungal strains on crude oil removal rates.In the three soil temperatures tested(20℃,25℃,and 30℃),the highest average crude oil removal rate was at 25℃(74.8%)and the lowest at 30℃(49.4%).At each temperature,variations in the addition of organic matter and oxygen providers had significant effects on crude oil removal rate.Variations in surfactant addition was significant at 20℃ and 25℃ but insignificant at 30℃.Given the same surfactant treatment,variations in temperature,organic additives,and oxygen providers was significant for crude oil removal rate.Treatments without surfactants and treatments with Tween80 exhibited their highest crude oil removal rates at 25℃.However,treatments that included the SDS surfactant exhibited their highest crude oil removal rates at 30℃.Amongst the treatments without surfactants,treatments with corn cob addition had the highest crude oil removal rates,and with surfactants,treatments that included the organic fertilizer exhibited the highest crude oil removal rates.Given the same organic fertilizer treatment,the highest crude oil removal rate was at 25℃.At each level of oxygen availability,the maximum crude oil removal rate always occurred at 25℃,and the treatments that included organic fertilizer exhibited the highest crude oil removal rates.Amongst the treatments without oxygen providers,treatments without surfactants had the highest crude oil removal rates,and with an oxygen provider,treatments with SDS addition exhibited the highest crude oil removal rates.Based on the crude oil removal rates of the treatments,we determined that S_(1)W_(1)O_(1)(addition of Tween80,organic fertilizers,and H_(2)O_(2))was optimum for remediating petroleum-contaminated forest soils in cold,high-altitude regions.This study is helpful to vegetation restoration and reforestation on petroleum contaminated forestlands.展开更多
Heavy metal contamination of soil and water is one of the most prominent environmental issues worldwide.Through bioaccumulation and biomagnification of the food chain,heavy metals can be enriched hundreds of times and...Heavy metal contamination of soil and water is one of the most prominent environmental issues worldwide.Through bioaccumulation and biomagnification of the food chain,heavy metals can be enriched hundreds of times and eventually enter the human body,posing a major threat to human health.Biomineralization has the greatest potential to become an efficient and environmentally friendly heavy metal remediation technology and has received much attention in recent decades.This review summarizes the latest progress of biomineralization technology on carbonate precipitation and phosphate precipitation in heavy metal remediation.Both microorganisms(including bacteria and fungi)and enzymes can induce carbonate and phosphate precipitation,converting the free heavy metal ions into insoluble salts.However,the mechanisms of the heavy metal remediation are significantly different.For example,urea hydrolysis,which occurs intracellularly when urease-producing bacteria(UPB)are used,is the most commonly used mechanism for carbonate precipitation based bioremediation.In contrast,phosphate solubilization by either enzymes or organic acids secreted by phosphate solubilizing bacteria(PSB)is extracellular,and both soluble and insoluble phosphorus can be decomposed by PSB.Moreover,some influencing factors such as the different species of microorganism,heavy metals and some environmental conditions that may affect the bioremediation of heavy metals were also summarized in this paper.The challenges of biomineralization based heavy metal remediation are also discussed.Based on the reviews of previous studies,a comprehensive understanding of heavy metal removal through microorganism can be increased,and thus promotes the applications of biomineralization technology in the treatment of large-scale heavy metal contaminated sites.展开更多
The soil contaminated by petroleum hydrocarbons and main methods of cleaning the pollutants were dealt with in the paper.The key principles,technological types, effect factors ,consolidation of the technology,present ...The soil contaminated by petroleum hydrocarbons and main methods of cleaning the pollutants were dealt with in the paper.The key principles,technological types, effect factors ,consolidation of the technology,present situation and development of the bioremediation of the soil contaminated by petroleum hydrocarbons were presented.展开更多
文摘Effects of bioremediation on microbial communities in soils contaminated with petroleum hydrocarbons are a scientific problem to be solved. Changes in dominate microbial species and the total amount of microorganisms including bacteria and fungi in rhizospheric soils after bioremediation were thus evaluated using field bioremediation experiments. The results showed that there were changed dominant microorganisms including 11 bacterial strains which are mostly Gram positive bacteria and 6 fungal species which were identified. The total amount of microorganisms including bacteria and fungi increased after bioremediation of microbial agents combined with planting maize. On the contrary, fungi in rhizospheric soils were inhibited by adding microbial agents combined with planting soybean.
基金the National Natural Science Foundation of China (No.30570340)the Foundation of the Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology, SOA (No.200701) Cheung Kong Scholar Program of the Education Ministry of China.
文摘The bioremediation potential of bacteria indigenous to soils of the Yellow River Delta in China was evaluated as a treatment option for soil remediation. Petroleum hydrocarbon degraders were isolated from contaminated soil samples from the Yellow River Delta. Four microbial communities and eight isolates were obtained. The optimal temperature, salinity, pH, and the ratios of C, N, and P (C:N:P) for the maximum biodegradation of diesel oil, crude oil, n-alkanes, and polyaromatic hydrocarbons by indigenous bacteria were determined, and the kinetics changes in microbial communities were monitored. In general, the mixed microbial consortia demonstrated wider catabolic versatility and faster overall rate of hydrocarbon degradation than individual isolates. Our experimental results demonstrated the feasibility of biodegradation of petroleum hydrocarbon by indigenous bacteria for soil remediation in the Yellow River Delta.
基金supported by the Major Research Plan of Tianjin (No.16YFXTSF00460)the National Natural Science Foundation of China (No.21878220)
文摘Diversity in bacterial communities was investigated along a petroleum hydrocarbon content gradient(0-0.4043 g/g)in surface(5-10 cm)and subsurface(35-40 cm)petroleum-contaminated soil samples from the Dagang Oilfield,China.Using 16S rRNA Illumina high-throughput sequencing technology and several statistical methods,the bacterial diversity of the soil was studied.Subsequently,the environmental parameters were measured to analyze its relationship with the community variation.Nonmetric multidimensional scaling and analysis of similarities indicated a significant difference in the structure of the bacterial community between the nonpetroleum-contaminated surface and subsurface soils,but no differences were observed in different depths of petroleum-contaminated soil.Meanwhile,many significant correlations were obtained between diversity in soil bacterial community and physicochemical properties.Total petroleum hydrocarbon,total organic carbon,and total nitrogen were the three important factors that had the greatest impacts on the bacterial community distribution in the long-term petroleum-contaminated soils.Our research has provided references for the bacterial community distribution along a petroleum gradient in both surface and subsurface petroleum-contaminated soils of oilfield areas.
基金This study was funded by the“948”project of The State Forestry Administration,“Microbial remediation of oil-polluted soil in Daqing Area”(2008-4-34)Special Fund project of basic Scientifi c Research operating Fee of Central Universities(2572014BA16).
文摘Although petroleum is an important source of energy and an economic driver of growth,it is also a major soil pollutant that has destroyed large swathes of vegetation and forest cover.Therefore,it is vital to develop affordable and efficient methods for the bioremediation of petroleum-contaminated forest soils to restore vegetation and improve tree survival rates.In this study,bioremediation experiments were performed in an electrically heated thermostatic reactor to test the effects of organic matter additives,surfactants,and oxygen providers of nine hydrocarbon-degrading fungal strains on crude oil removal rates.In the three soil temperatures tested(20℃,25℃,and 30℃),the highest average crude oil removal rate was at 25℃(74.8%)and the lowest at 30℃(49.4%).At each temperature,variations in the addition of organic matter and oxygen providers had significant effects on crude oil removal rate.Variations in surfactant addition was significant at 20℃ and 25℃ but insignificant at 30℃.Given the same surfactant treatment,variations in temperature,organic additives,and oxygen providers was significant for crude oil removal rate.Treatments without surfactants and treatments with Tween80 exhibited their highest crude oil removal rates at 25℃.However,treatments that included the SDS surfactant exhibited their highest crude oil removal rates at 30℃.Amongst the treatments without surfactants,treatments with corn cob addition had the highest crude oil removal rates,and with surfactants,treatments that included the organic fertilizer exhibited the highest crude oil removal rates.Given the same organic fertilizer treatment,the highest crude oil removal rate was at 25℃.At each level of oxygen availability,the maximum crude oil removal rate always occurred at 25℃,and the treatments that included organic fertilizer exhibited the highest crude oil removal rates.Amongst the treatments without oxygen providers,treatments without surfactants had the highest crude oil removal rates,and with an oxygen provider,treatments with SDS addition exhibited the highest crude oil removal rates.Based on the crude oil removal rates of the treatments,we determined that S_(1)W_(1)O_(1)(addition of Tween80,organic fertilizers,and H_(2)O_(2))was optimum for remediating petroleum-contaminated forest soils in cold,high-altitude regions.This study is helpful to vegetation restoration and reforestation on petroleum contaminated forestlands.
基金support by the National Natural Science Foundation of China(NSFC)(Grant No.52178319,52108307,52078236,51878313,51708243)the Natural Science Foundation of Fujian Province,China(Grant No.2022J05020,2022J05127).
文摘Heavy metal contamination of soil and water is one of the most prominent environmental issues worldwide.Through bioaccumulation and biomagnification of the food chain,heavy metals can be enriched hundreds of times and eventually enter the human body,posing a major threat to human health.Biomineralization has the greatest potential to become an efficient and environmentally friendly heavy metal remediation technology and has received much attention in recent decades.This review summarizes the latest progress of biomineralization technology on carbonate precipitation and phosphate precipitation in heavy metal remediation.Both microorganisms(including bacteria and fungi)and enzymes can induce carbonate and phosphate precipitation,converting the free heavy metal ions into insoluble salts.However,the mechanisms of the heavy metal remediation are significantly different.For example,urea hydrolysis,which occurs intracellularly when urease-producing bacteria(UPB)are used,is the most commonly used mechanism for carbonate precipitation based bioremediation.In contrast,phosphate solubilization by either enzymes or organic acids secreted by phosphate solubilizing bacteria(PSB)is extracellular,and both soluble and insoluble phosphorus can be decomposed by PSB.Moreover,some influencing factors such as the different species of microorganism,heavy metals and some environmental conditions that may affect the bioremediation of heavy metals were also summarized in this paper.The challenges of biomineralization based heavy metal remediation are also discussed.Based on the reviews of previous studies,a comprehensive understanding of heavy metal removal through microorganism can be increased,and thus promotes the applications of biomineralization technology in the treatment of large-scale heavy metal contaminated sites.
文摘The soil contaminated by petroleum hydrocarbons and main methods of cleaning the pollutants were dealt with in the paper.The key principles,technological types, effect factors ,consolidation of the technology,present situation and development of the bioremediation of the soil contaminated by petroleum hydrocarbons were presented.
