Activity of a crude biosurfactant extracted from the culture fluid of Serratia sp. that was isolated from riverbed soil was shown to increase in proportion to the cultivation time, and was higher at pH 8 than at pH 7....Activity of a crude biosurfactant extracted from the culture fluid of Serratia sp. that was isolated from riverbed soil was shown to increase in proportion to the cultivation time, and was higher at pH 8 than at pH 7. Serratia sp. grew in the mineral-based medium with soybean oil but was not with kerosene-diesel. The petroleum-degrading bacteria—Acinetobacter sp., Pseudomonas sp., Paracoccus sp., and Cupriavidus sp.—were isolated from a specially designed enrichment culture. The efficiency of mineralization of wastewater contaminated with kerosene and diesel (WKD) by the petroleum-degrading bacterial community (PDBC) was enhanced significantly by addition of the crude biosurfactant. The efficiency of mineralization of the WKD was also about 2 times boosted by co-culture of Serratia sp. and PDBC. Bacterial community of Serratia sp. and PDBC co-cultivated in the WKD was maintained for at least 8 days according to the TGGE pattern of 16S rDNA obtained from the bacterial culture. In conclusion, the co-culture of Serratia sp. and PDBC is an applicable technique for the mineralization of wastewater contaminated with petroleum, which may substitute for chemical or biological surfactant.展开更多
The growth characteristics of petroleum-degrader BS-8（Bacillus sp.） and the factors influencing its biosurfactant production were tested; the biosurfactant releasing mode of BS-8 was speculated by measuring OD600, s...The growth characteristics of petroleum-degrader BS-8（Bacillus sp.） and the factors influencing its biosurfactant production were tested; the biosurfactant releasing mode of BS-8 was speculated by measuring OD600, surface tension and oil displacement of fermentation broth; and the effects of carbon source, nitrogen source, temperature, p H, and Na Cl concentration on biosurfactant production by BS-8 were observed in this study. The results showed that the biosurfactant releasing mode of BS-8 was growth-correlated, the surface tension of BS-8 fermentation broth declined with the total bacteria increasing, and the oil displacement was positive correlated with biosurfactant content in fermentation broth; and the optimal culture conditions for effective biosurfactant production included： glucose as carbon source,yeast extract as nitrogen source, Na Cl concentration of 2%, p H of 7.0 and temperature at 30 ℃.展开更多
文摘Activity of a crude biosurfactant extracted from the culture fluid of Serratia sp. that was isolated from riverbed soil was shown to increase in proportion to the cultivation time, and was higher at pH 8 than at pH 7. Serratia sp. grew in the mineral-based medium with soybean oil but was not with kerosene-diesel. The petroleum-degrading bacteria—Acinetobacter sp., Pseudomonas sp., Paracoccus sp., and Cupriavidus sp.—were isolated from a specially designed enrichment culture. The efficiency of mineralization of wastewater contaminated with kerosene and diesel (WKD) by the petroleum-degrading bacterial community (PDBC) was enhanced significantly by addition of the crude biosurfactant. The efficiency of mineralization of the WKD was also about 2 times boosted by co-culture of Serratia sp. and PDBC. Bacterial community of Serratia sp. and PDBC co-cultivated in the WKD was maintained for at least 8 days according to the TGGE pattern of 16S rDNA obtained from the bacterial culture. In conclusion, the co-culture of Serratia sp. and PDBC is an applicable technique for the mineralization of wastewater contaminated with petroleum, which may substitute for chemical or biological surfactant.
基金Supported by Basic and Cutting-edge Technique Research of Henan Province(152300410092)Key Program in Science and Technology of Education Department of Henan Province(15A210020,15B180002,15B180016)~~
文摘The growth characteristics of petroleum-degrader BS-8（Bacillus sp.） and the factors influencing its biosurfactant production were tested; the biosurfactant releasing mode of BS-8 was speculated by measuring OD600, surface tension and oil displacement of fermentation broth; and the effects of carbon source, nitrogen source, temperature, p H, and Na Cl concentration on biosurfactant production by BS-8 were observed in this study. The results showed that the biosurfactant releasing mode of BS-8 was growth-correlated, the surface tension of BS-8 fermentation broth declined with the total bacteria increasing, and the oil displacement was positive correlated with biosurfactant content in fermentation broth; and the optimal culture conditions for effective biosurfactant production included： glucose as carbon source,yeast extract as nitrogen source, Na Cl concentration of 2%, p H of 7.0 and temperature at 30 ℃.
