To investigate the chemical constituents of the roots ofPolygala sibirica L. The separation and purification were performed by solvent extraction and repeated chromatography with silica gel, Sephadex LH-20, ODS column...To investigate the chemical constituents of the roots ofPolygala sibirica L. The separation and purification were performed by solvent extraction and repeated chromatography with silica gel, Sephadex LH-20, ODS columns, and semiprep. HPLC. The structures were elucidated by spectral analysis. Twelve known compounds were isolated and identified as tenuifoliside A （1）, tenuifoliside B （2）, glomeratose A （3）, 3＇,6-disinapoyl sucrose （4）, sibiricose A5 （5）, sibiricose A6 （6）, sibiricose A1 （7）, sibiricose A2 （8）, polygalatenoside E （9）, 1-O-L-arabinopyranosyl-O-（6→1）-β-D-glucopyranosyl-salicylate （10）, canthoside A （11）, and methyl- 3,4,5-trimethoxycinnamate （12）. Compound 11 was obtained from genus Polygala for the first time, and compounds 2, 9, 10 and 12 were isolated from this plant for the first time.展开更多
Siberian apricot(Prunus sibirica L.)is a traditional nut tree species in East Asia and Siberia and is a possible contribution to healthy diets.However,it has attracted little research attention and information on the ...Siberian apricot(Prunus sibirica L.)is a traditional nut tree species in East Asia and Siberia and is a possible contribution to healthy diets.However,it has attracted little research attention and information on the nutritional value of its kernel is limited.In this study,the profile,nutritional value and variation of amino acids were investigated in eight provenances.The kernels contained29×10-2 g/g protein and were rich in glutamic acid(26.5%),aspartic acid(11.3%)and arginine(10.1%).They showed higher essential amino acids(EAA)than similar protein values for almonds.The variation coefficients of amino acids ranged from 3.8 to 43.7%,and the levels of seven amino acids were significantly different among the eight provenances.The proportion of essential amino acids to total amino acids and amino acid score were also quite different.Protein was negatively correlated with some amino acids and protein quality values.In conclusion,there were two superior provenances(Wanjiagou and Horinger County)with high EAA contents and protein quality,and could be used in the large-scale development of this species.展开更多
A new iridoid glycoside,versibirioside（1）,and a known iridoid glycoside,verbaspinoside（2）,were isolated from the whole plant of Veronica sibirica L.Their structures were elucidated by spectroscopic methods,includi...A new iridoid glycoside,versibirioside（1）,and a known iridoid glycoside,verbaspinoside（2）,were isolated from the whole plant of Veronica sibirica L.Their structures were elucidated by spectroscopic methods,including 2D-NMR spectra.展开更多
Root growth and spatial and temporal distribution in the 0-100 cm soil profiles of three common annual halophytes Salsola subcrassa, Suaeda acuminate and Petrosimonia sibirica distributed in a saline desert in north- ...Root growth and spatial and temporal distribution in the 0-100 cm soil profiles of three common annual halophytes Salsola subcrassa, Suaeda acuminate and Petrosimonia sibirica distributed in a saline desert in north- ern Xinjiang, China were studied in 2009 and 2010. The results showed that the root systems of the three halo- phytes were of the taproot type, vertically distributed in the 90-cm soil profile, and were deepest in late July. Their taproots reached maximum depth rapidly, early in the growth period, but with rare lateral roots. They were then dug out in an orderly way, from bottom to top, exhibiting vertical development first and then horizontal development. The distribution of specific root length, which reflects the characteristics of the feeder root, was gradually increased from top to bottom, whereas root weight displayed an opposite distribution pattern. The root length distribution of the three halophytes was concentrated （62% to 76%） in the middle soil profile （20-60 cm）, with less distribution in the surface （0-20 cm） and bottom （60-90 cm） soil profiles. The results indicated that the roots of the three annual halophytes grew rapidly into the deeper soil layer after germination, which ensured the plant survival and uptake of water and nutrition, and thus built up a strong tolerance to an arid, high-salt environment.展开更多
Objective To investigate the anti-oxidant and anti-inflammatory effects of ethanol extract of Polygala sibirica L.var megalopha Fr.(EEP)on RAW264.7 mouse macrophages.Methods RAW264.7 cells were pretreated with 0–200&...Objective To investigate the anti-oxidant and anti-inflammatory effects of ethanol extract of Polygala sibirica L.var megalopha Fr.(EEP)on RAW264.7 mouse macrophages.Methods RAW264.7 cells were pretreated with 0–200µg/mL EEP or vehicle for 2 h prior to exposure to 1µg/mL lipopolysaccharide(LPS)for 24 h.Nitric oxide(NO)and prostaglandin(PGE2)production were determined by Griess reagent and enzyme-linked immunosorbent assay(ELISA),respectively.The mRNA levels of inducible nitric oxide synthase(iNOS),cyclooxygenase-2(COX-2),tumor necrosis factorα(TNF-α),interleukin-1beta(IL-1β),and IL-6 were determined using reverse transcription polymerase chain reaction(RT-PCR).Western blot assay was used to determine the protein expressions of iNOS,COX-2,phosphorylation of extracellular regulated protein kinases(ERK1/2),c-Jun N-terminal kinase(JNK),inhibitory subunit of nuclear factor Kappa B alpha(IκB-α)and p38.Immunofluorescence was used to observe the nuclear expression of nuclear factor-κB p65(NF-κB p65).Additionally,the anti-oxidant potential of EEP was evaluated by reactive oxygen species(ROS)production and the activities of catalase(CAT)and superoxide dismutase(SOD).The 2,2-diphenyl-1-picrylhydrazyl(DPPH),hydroxyl(OH),superoxide anion(O2−)radical and nitrite scavenging activity were also measured.Results The total polyphenol and flavonoid contents of EEP were 23.50±2.16 mg gallic acid equivalent/100 g and 43.78±3.81 mg rutin equivalent/100 g.With EEP treatment(100 and 150µg/mL),there was a notable decrease in NO and PGE2 production induced by LPS in RAW264.7 cells by downregulation of iNOS and COX-2 mRNA and protein expressions(P<0.01 or P<0.05).Furthermore,with EEP treatment(150µg/mL),there was a decrease in the mRNA expression levels of TNF-α,IL-1βand IL-6,as well as in the phosphorylation of ERK,JNK and p38 mitogen-activated protein kinase(MAPK,P<0.01 or P<0.05),by blocking the nuclear translocation of NF-κB p65 in LPS-stimulated cells.In addition,EEP(100 and 150µg/mL)led to an increase in the anti-oxidant enzymes activity of SOD and CAT,with a concomitant decrease in ROS production(P<0.01 or P<0.05).EEP also indicated the DPPH,OH,O2−radical and nitrite scavenging activity.Conclusion EEP inhibited inflammatory responses in activated macrophages through blocking MAPK/NF-κB pathway and protected against oxidative stress.展开更多
目的研究轮叶婆婆纳V eron ica sibirica中二萜类成分的体外抗癌作用。方法以T J905和H eL a细胞为研究对象,采用M TT法对获得的化合物进行抗癌活性初筛并对初筛活性高的化合物进行剂量依赖性实验,测定其IC50值。结果对轮叶婆婆纳石油...目的研究轮叶婆婆纳V eron ica sibirica中二萜类成分的体外抗癌作用。方法以T J905和H eL a细胞为研究对象,采用M TT法对获得的化合物进行抗癌活性初筛并对初筛活性高的化合物进行剂量依赖性实验,测定其IC50值。结果对轮叶婆婆纳石油醚及醋酸乙酯提取物中的7个二萜类化合物:二氢丹参酮Ⅰ(Ⅰ)、丹参酮Ⅰ(Ⅱ)、丹参酮ⅡA(Ⅲ)、隐丹参酮(Ⅳ)、婆婆纳对醌A(Ⅴ)、婆婆纳对醌B(Ⅵ)、弥罗松酚(Ⅶ)进行了体外抗肿瘤活性考察,表明化合物Ⅰ和Ⅳ具有显著的抗癌活性,化合物Ⅰ对T J905及H eL a细胞生长的IC50值分别为1.038、1.816μg/mL,化合物Ⅳ对T J905及H eL a细胞生长的IC50值分别为3.637、4.391μg/mL。结论轮叶婆婆纳中的二氢丹参酮Ⅰ和隐丹参酮对T J905及H eL a细胞具有较强的生长抑制活性。展开更多
基金Program for Changjiang Scholar and Innovative Team in University(Grant No.985-2-063-112)Program for New Century Excellent Talents in University(Grant No.985-2-102-113).
文摘To investigate the chemical constituents of the roots ofPolygala sibirica L. The separation and purification were performed by solvent extraction and repeated chromatography with silica gel, Sephadex LH-20, ODS columns, and semiprep. HPLC. The structures were elucidated by spectral analysis. Twelve known compounds were isolated and identified as tenuifoliside A （1）, tenuifoliside B （2）, glomeratose A （3）, 3＇,6-disinapoyl sucrose （4）, sibiricose A5 （5）, sibiricose A6 （6）, sibiricose A1 （7）, sibiricose A2 （8）, polygalatenoside E （9）, 1-O-L-arabinopyranosyl-O-（6→1）-β-D-glucopyranosyl-salicylate （10）, canthoside A （11）, and methyl- 3,4,5-trimethoxycinnamate （12）. Compound 11 was obtained from genus Polygala for the first time, and compounds 2, 9, 10 and 12 were isolated from this plant for the first time.
基金This study is supported by Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry (CAFYBB2017ZA004-4)
文摘Siberian apricot(Prunus sibirica L.)is a traditional nut tree species in East Asia and Siberia and is a possible contribution to healthy diets.However,it has attracted little research attention and information on the nutritional value of its kernel is limited.In this study,the profile,nutritional value and variation of amino acids were investigated in eight provenances.The kernels contained29×10-2 g/g protein and were rich in glutamic acid(26.5%),aspartic acid(11.3%)and arginine(10.1%).They showed higher essential amino acids(EAA)than similar protein values for almonds.The variation coefficients of amino acids ranged from 3.8 to 43.7%,and the levels of seven amino acids were significantly different among the eight provenances.The proportion of essential amino acids to total amino acids and amino acid score were also quite different.Protein was negatively correlated with some amino acids and protein quality values.In conclusion,there were two superior provenances(Wanjiagou and Horinger County)with high EAA contents and protein quality,and could be used in the large-scale development of this species.
文摘A new iridoid glycoside,versibirioside（1）,and a known iridoid glycoside,verbaspinoside（2）,were isolated from the whole plant of Veronica sibirica L.Their structures were elucidated by spectroscopic methods,including 2D-NMR spectra.
基金the Chinese Academy of Sciences Action-plan for West Development (KZCX2-XB3-07) for financial support"Western Light" Talents Training Program of Chinese Academy of Sciences (XBBS200811)
文摘Root growth and spatial and temporal distribution in the 0-100 cm soil profiles of three common annual halophytes Salsola subcrassa, Suaeda acuminate and Petrosimonia sibirica distributed in a saline desert in north- ern Xinjiang, China were studied in 2009 and 2010. The results showed that the root systems of the three halo- phytes were of the taproot type, vertically distributed in the 90-cm soil profile, and were deepest in late July. Their taproots reached maximum depth rapidly, early in the growth period, but with rare lateral roots. They were then dug out in an orderly way, from bottom to top, exhibiting vertical development first and then horizontal development. The distribution of specific root length, which reflects the characteristics of the feeder root, was gradually increased from top to bottom, whereas root weight displayed an opposite distribution pattern. The root length distribution of the three halophytes was concentrated （62% to 76%） in the middle soil profile （20-60 cm）, with less distribution in the surface （0-20 cm） and bottom （60-90 cm） soil profiles. The results indicated that the roots of the three annual halophytes grew rapidly into the deeper soil layer after germination, which ensured the plant survival and uptake of water and nutrition, and thus built up a strong tolerance to an arid, high-salt environment.
文摘Objective To investigate the anti-oxidant and anti-inflammatory effects of ethanol extract of Polygala sibirica L.var megalopha Fr.(EEP)on RAW264.7 mouse macrophages.Methods RAW264.7 cells were pretreated with 0–200µg/mL EEP or vehicle for 2 h prior to exposure to 1µg/mL lipopolysaccharide(LPS)for 24 h.Nitric oxide(NO)and prostaglandin(PGE2)production were determined by Griess reagent and enzyme-linked immunosorbent assay(ELISA),respectively.The mRNA levels of inducible nitric oxide synthase(iNOS),cyclooxygenase-2(COX-2),tumor necrosis factorα(TNF-α),interleukin-1beta(IL-1β),and IL-6 were determined using reverse transcription polymerase chain reaction(RT-PCR).Western blot assay was used to determine the protein expressions of iNOS,COX-2,phosphorylation of extracellular regulated protein kinases(ERK1/2),c-Jun N-terminal kinase(JNK),inhibitory subunit of nuclear factor Kappa B alpha(IκB-α)and p38.Immunofluorescence was used to observe the nuclear expression of nuclear factor-κB p65(NF-κB p65).Additionally,the anti-oxidant potential of EEP was evaluated by reactive oxygen species(ROS)production and the activities of catalase(CAT)and superoxide dismutase(SOD).The 2,2-diphenyl-1-picrylhydrazyl(DPPH),hydroxyl(OH),superoxide anion(O2−)radical and nitrite scavenging activity were also measured.Results The total polyphenol and flavonoid contents of EEP were 23.50±2.16 mg gallic acid equivalent/100 g and 43.78±3.81 mg rutin equivalent/100 g.With EEP treatment(100 and 150µg/mL),there was a notable decrease in NO and PGE2 production induced by LPS in RAW264.7 cells by downregulation of iNOS and COX-2 mRNA and protein expressions(P<0.01 or P<0.05).Furthermore,with EEP treatment(150µg/mL),there was a decrease in the mRNA expression levels of TNF-α,IL-1βand IL-6,as well as in the phosphorylation of ERK,JNK and p38 mitogen-activated protein kinase(MAPK,P<0.01 or P<0.05),by blocking the nuclear translocation of NF-κB p65 in LPS-stimulated cells.In addition,EEP(100 and 150µg/mL)led to an increase in the anti-oxidant enzymes activity of SOD and CAT,with a concomitant decrease in ROS production(P<0.01 or P<0.05).EEP also indicated the DPPH,OH,O2−radical and nitrite scavenging activity.Conclusion EEP inhibited inflammatory responses in activated macrophages through blocking MAPK/NF-κB pathway and protected against oxidative stress.
文摘目的研究轮叶婆婆纳V eron ica sibirica中二萜类成分的体外抗癌作用。方法以T J905和H eL a细胞为研究对象,采用M TT法对获得的化合物进行抗癌活性初筛并对初筛活性高的化合物进行剂量依赖性实验,测定其IC50值。结果对轮叶婆婆纳石油醚及醋酸乙酯提取物中的7个二萜类化合物:二氢丹参酮Ⅰ(Ⅰ)、丹参酮Ⅰ(Ⅱ)、丹参酮ⅡA(Ⅲ)、隐丹参酮(Ⅳ)、婆婆纳对醌A(Ⅴ)、婆婆纳对醌B(Ⅵ)、弥罗松酚(Ⅶ)进行了体外抗肿瘤活性考察,表明化合物Ⅰ和Ⅳ具有显著的抗癌活性,化合物Ⅰ对T J905及H eL a细胞生长的IC50值分别为1.038、1.816μg/mL,化合物Ⅳ对T J905及H eL a细胞生长的IC50值分别为3.637、4.391μg/mL。结论轮叶婆婆纳中的二氢丹参酮Ⅰ和隐丹参酮对T J905及H eL a细胞具有较强的生长抑制活性。
