Both glial cells and glia scar greatly affect the development of spinal cord injury and have become hot spots in research on spinal cord injury treatment.The cellular deposition of dense extracellular matrix proteins ...Both glial cells and glia scar greatly affect the development of spinal cord injury and have become hot spots in research on spinal cord injury treatment.The cellular deposition of dense extracellular matrix proteins such as chondroitin sulfate proteoglycans inside and around the glial scar is known to affect axonal growth and be a major obstacle to autogenous repair.These proteins are thus candidate targets for spinal cord injury therapy.Our previous studies demonstrated that 810 nm photo biomodulation inhibited the formation of chondroitin sulfate proteoglycans after spinal cord injury and greatly improved motor function in model animals.However,the specific mechanism and potential targets involved remain to be clarified.In this study,to investigate the therapeutic effect of photo biomodulation,we established a mouse model of spinal cord injury by T9 clamping and irradiated the injury site at a power density of 50 mW/cm~2 for 50 minutes once a day for 7 consecutive days.We found that photobiomodulation greatly restored motor function in mice and down regulated chondroitin sulfate proteoglycan expression in the injured spinal cord.Bioinformatics analysis revealed that photobiomodulation inhibited the expression of proteoglycan-related genes induced by spinal cord injury,and versican,a type of proteoglycan,was one of the most markedly changed molecules.Immunofluorescence staining showed that after spinal cord injury,versican was present in astrocytes in spinal cord tissue.The expression of versican in primary astrocytes cultured in vitro increased after inflammation induction,whereas photobiomodulation inhibited the expression of ve rsican.Furthermore,we found that the increased levels of p-Smad3,p-P38 and p-Erk in inflammatory astrocytes were reduced after photobiomodulation treatment and after delivery of inhibitors including FR 180204,(E)-SIS3,and SB 202190.This suggests that Sma d 3/Sox9 and MAP K/Sox9 pathways may be involved in the effects of photobiomodulation.In summary,our findings show that photobiomodulation modulates the expression of chondroitin sulfate proteoglycans,and versican is one of the key target molecules of photo biomodulation.MAPK/Sox9 and Smad3/Sox9 pathways may play a role in the effects of photo biomodulation on chondroitin sulfate proteoglycan accumulation after spinal cord injury.展开更多
To solve the problems in restoring sedimentary facies and predicting reservoirs in loose gas-bearing sediment,based on seismic sedimentologic analysis of the first 9-component S-wave 3D seismic dataset of China,a four...To solve the problems in restoring sedimentary facies and predicting reservoirs in loose gas-bearing sediment,based on seismic sedimentologic analysis of the first 9-component S-wave 3D seismic dataset of China,a fourth-order isochronous stratigraphic framework was set up and then sedimentary facies and reservoirs in the Pleistocene Qigequan Formation in Taidong area of Qaidam Basin were studied by seismic geomorphology and seismic lithology.The study method and thought are as following.Firstly,techniques of phase rotation,frequency decomposition and fusion,and stratal slicing were applied to the 9-component S-wave seismic data to restore sedimentary facies of major marker beds based on sedimentary models reflected by satellite images.Then,techniques of seismic attribute extraction,principal component analysis,and random fitting were applied to calculate the reservoir thickness and physical parameters of a key sandbody,and the results are satisfactory and confirmed by blind testing wells.Study results reveal that the dominant sedimentary facies in the Qigequan Formation within the study area are delta front and shallow lake.The RGB fused slices indicate that there are two cycles with three sets of underwater distributary channel systems in one period.Among them,sandstones in the distributary channels of middle-low Qigequan Formation are thick and broad with superior physical properties,which are favorable reservoirs.The reservoir permeability is also affected by diagenesis.Distributary channel sandstone reservoirs extend further to the west of Sebei-1 gas field,which provides a basis to expand exploration to the western peripheral area.展开更多
基金supported by the National Natural Science Foundation of China,Nos.81070996(to ZW),81572151(to XH)Shaanxi Provincial Key R&D Program,Nos.2020ZDLSF02-05(to ZW),2021ZDLSF02-10(to XH)+1 种基金Everest Project of Military Medicine of Air Force Medical University,No.2018RCFC02(to XH)Boosting Project of the First Affiliated Hospital of Air Force Medical University,No.XJZT19Z22(to ZW)。
文摘Both glial cells and glia scar greatly affect the development of spinal cord injury and have become hot spots in research on spinal cord injury treatment.The cellular deposition of dense extracellular matrix proteins such as chondroitin sulfate proteoglycans inside and around the glial scar is known to affect axonal growth and be a major obstacle to autogenous repair.These proteins are thus candidate targets for spinal cord injury therapy.Our previous studies demonstrated that 810 nm photo biomodulation inhibited the formation of chondroitin sulfate proteoglycans after spinal cord injury and greatly improved motor function in model animals.However,the specific mechanism and potential targets involved remain to be clarified.In this study,to investigate the therapeutic effect of photo biomodulation,we established a mouse model of spinal cord injury by T9 clamping and irradiated the injury site at a power density of 50 mW/cm~2 for 50 minutes once a day for 7 consecutive days.We found that photobiomodulation greatly restored motor function in mice and down regulated chondroitin sulfate proteoglycan expression in the injured spinal cord.Bioinformatics analysis revealed that photobiomodulation inhibited the expression of proteoglycan-related genes induced by spinal cord injury,and versican,a type of proteoglycan,was one of the most markedly changed molecules.Immunofluorescence staining showed that after spinal cord injury,versican was present in astrocytes in spinal cord tissue.The expression of versican in primary astrocytes cultured in vitro increased after inflammation induction,whereas photobiomodulation inhibited the expression of ve rsican.Furthermore,we found that the increased levels of p-Smad3,p-P38 and p-Erk in inflammatory astrocytes were reduced after photobiomodulation treatment and after delivery of inhibitors including FR 180204,(E)-SIS3,and SB 202190.This suggests that Sma d 3/Sox9 and MAP K/Sox9 pathways may be involved in the effects of photobiomodulation.In summary,our findings show that photobiomodulation modulates the expression of chondroitin sulfate proteoglycans,and versican is one of the key target molecules of photo biomodulation.MAPK/Sox9 and Smad3/Sox9 pathways may play a role in the effects of photo biomodulation on chondroitin sulfate proteoglycan accumulation after spinal cord injury.
基金Supported by the CNPC Science and Technology Projects(2022-N/G-47808,2023-N/G-67014)RIPED International Cooperation Project(19HTY5000008).
文摘To solve the problems in restoring sedimentary facies and predicting reservoirs in loose gas-bearing sediment,based on seismic sedimentologic analysis of the first 9-component S-wave 3D seismic dataset of China,a fourth-order isochronous stratigraphic framework was set up and then sedimentary facies and reservoirs in the Pleistocene Qigequan Formation in Taidong area of Qaidam Basin were studied by seismic geomorphology and seismic lithology.The study method and thought are as following.Firstly,techniques of phase rotation,frequency decomposition and fusion,and stratal slicing were applied to the 9-component S-wave seismic data to restore sedimentary facies of major marker beds based on sedimentary models reflected by satellite images.Then,techniques of seismic attribute extraction,principal component analysis,and random fitting were applied to calculate the reservoir thickness and physical parameters of a key sandbody,and the results are satisfactory and confirmed by blind testing wells.Study results reveal that the dominant sedimentary facies in the Qigequan Formation within the study area are delta front and shallow lake.The RGB fused slices indicate that there are two cycles with three sets of underwater distributary channel systems in one period.Among them,sandstones in the distributary channels of middle-low Qigequan Formation are thick and broad with superior physical properties,which are favorable reservoirs.The reservoir permeability is also affected by diagenesis.Distributary channel sandstone reservoirs extend further to the west of Sebei-1 gas field,which provides a basis to expand exploration to the western peripheral area.
基金National Natural Science Foundation of China(Nos.U2037601,51821001)Key Basic Research Project of the National Basic Strengthening Plan,China(No.2022-xxxx-ZD-093-xx)。
