Sense and Sensibility,one of Jane Austen's masterpieces,has undergone a long time study and the two sisters in the book—Elinor and Marianne have been researched from various kinds of perspectives except"mela...Sense and Sensibility,one of Jane Austen's masterpieces,has undergone a long time study and the two sisters in the book—Elinor and Marianne have been researched from various kinds of perspectives except"melancholy philosophy".This paper aims to analyze the two sisters in Sense and Sensibility under the guidance of"melancholy philosophy".展开更多
全球领先的PA66树脂和化学中间体生产商奥升德高性能材料有限公司日前宣布任命Phil Mc Divitt担任聚酰胺业务总裁。作为全球聚酰胺业务的负责人,Phil Mc Divitt将在美国休斯敦的奥升德总部任职,领导聚酰胺业务的三个生产基地和1800余名...全球领先的PA66树脂和化学中间体生产商奥升德高性能材料有限公司日前宣布任命Phil Mc Divitt担任聚酰胺业务总裁。作为全球聚酰胺业务的负责人,Phil Mc Divitt将在美国休斯敦的奥升德总部任职,领导聚酰胺业务的三个生产基地和1800余名员工。同时他也将成为奥升德管理团队的一员,向公司首席执行官Fred Poses汇报。展开更多
文摘Sense and Sensibility,one of Jane Austen's masterpieces,has undergone a long time study and the two sisters in the book—Elinor and Marianne have been researched from various kinds of perspectives except"melancholy philosophy".This paper aims to analyze the two sisters in Sense and Sensibility under the guidance of"melancholy philosophy".
文摘全球领先的PA66树脂和化学中间体生产商奥升德高性能材料有限公司日前宣布任命Phil Mc Divitt担任聚酰胺业务总裁。作为全球聚酰胺业务的负责人,Phil Mc Divitt将在美国休斯敦的奥升德总部任职,领导聚酰胺业务的三个生产基地和1800余名员工。同时他也将成为奥升德管理团队的一员,向公司首席执行官Fred Poses汇报。
文摘目的:构建人血管抑素K1-3的重组酵母胞内表达质粒PH IL-D2/K1-3,获得重组目的蛋白。方法:从胎儿肝脏组织用RT-PCR方法扩增人血管抑素K1-3目的基因,克隆至酵母胞内表达质粒PH IL-D2中,线性化后转化到毕赤酵母GS115胞内,经PCR和Sourthern杂交筛选出阳性转化子,并用甲醇诱导表达。表达产物进行SDS-PAGE(12%)和W estern b lot检测,经赖氨酸亲和层析柱纯化后,用Lowry法测定蛋白含量,并进行生物活性测定。结果:SDS-PAGE和W estern b lot结果显示,表达蛋白质的相对分子量为30kd左右,低糖基化。Lowry法测定蛋白表达量为6.8mg/L。活性测定实验表明重组血管抑素能特异抑制人血管内皮细胞的增殖。结论:在毕赤酵母GS115胞内成功表达了人血管抑素K1-3重组蛋白。