A lthough advanced stage aggressive non-Hodgkin'slymphomas and Hodgkin's disease are thought to be che-motherapy-responsive cancers, a considerable number of patients either relapse or never attain a remission...A lthough advanced stage aggressive non-Hodgkin'slymphomas and Hodgkin's disease are thought to be che-motherapy-responsive cancers, a considerable number of patients either relapse or never attain a remission. High-dose therapy(HDT) followed by autologous stem cell transplantation(ASCT) is often the only possibility of cure for most of these patients. However, many controversial issues still remain with respect to HDT/ASCT for lymphomas, including its role for, the optimal timing of transplantation, the best conditioning regimen and the potential use of localized radiotherapy or immunologic methods to decrease post-transplant recurrence. Recently, mainly due to the unavailability of carmustine, several novel conditioning protocols have been clinically developed, with the aim of improving the overall outcome by enhancing the anti-lymphoma effect and, at the same time, by reducing short and long-term toxicity. Furthermore, the better safety profiles of novel approaches would definitively allow patients aged more than 65-70 years to benefit from this therapeutic option. In this review, we will briefly discuss the most relevant and recent data available regarding HDT/ASCT in lymphomas.展开更多
Objective:To evaluate the antiniicrobial polenlial of different extracts of the endophytic fungus Phoma sp.and the tentative identification of their active constituents.Methods:The extract and compounds were screened ...Objective:To evaluate the antiniicrobial polenlial of different extracts of the endophytic fungus Phoma sp.and the tentative identification of their active constituents.Methods:The extract and compounds were screened for antimicrobial activity using the Agar Well Diffusion Method.Four compounds were purified using column chromatography and tlieir structures were assigned using～1H and～(13)C NMR spectra,DEPT,2D COSY,HMQC and HMBC experiments.Results:The ethyl acetate fraction of Phoma sp.showed good antifungal,antibacterial,and algicidal properties.One new dihydrofuran derivative,named phomafuranol(1),together with tliree known compounds,phomalacton(2),(3R)-5-hydroxymellein(3)and emodin(4)were isolated from the ethyl acetate fraction of Phoma sp.Preliminary studies indicated that phomalacton(2)displayed strong antibacterial,good antifungal and antialgal activities.Similarly(3R)-5-hydroxymellein(3)and emodin(4)showed good antifungal,antibacterial and algicidal properties.Conclusions:Antimicrobial activities of the ethyl acetate fraction of the endophytic fungus Phoma sp.and isolated compounds clearly demonstrate that Phoma sp.and its active compounds represent a great potential for the food,cosmetic and pharmaceutical industries.展开更多
Blackleg disease caused by Phoma lingam is an important disease of oil seed rape (Brassica napus) causing losses up to 95%. The efficacy of microbial antagonists against P. lingam in greenhouse was tested. Serratia pl...Blackleg disease caused by Phoma lingam is an important disease of oil seed rape (Brassica napus) causing losses up to 95%. The efficacy of microbial antagonists against P. lingam in greenhouse was tested. Serratia plymuthica HRO-C48 and Gliocladium catenulatum J1446 were able to reduce the disease intensity of OSR cotelydones infested with P. lingam at rates 44% and 52% respectively. The reduction of the infestation of the root collar in BBCH14/15 was evaluated as well. Plants treated with a suspension of the antagonists (2 × 105 cfu/plant) and inoculated with either pycnidiospore suspension (2 × 107 cfu/ml) or agar disks grown with P. lingam mycelium, showed a reduced infestation rate of 53% - 93% in the presence of S. plymuthica and 46% - 77% in the presence of G. catenulatum. The efficacy of the antagonist depends highly on their concentration inside OSR seeds. Below 105 cfu/seed no significant difference was recorded between control untreated and infested plants.展开更多
Twenty-one cases of primary gastric lym-phoma were compared with gastric cancer inorder to find out their differences in clinicaland endoscopic manifestations. The results showed that little clinical dif-ference betwe...Twenty-one cases of primary gastric lym-phoma were compared with gastric cancer inorder to find out their differences in clinicaland endoscopic manifestations. The results showed that little clinical dif-ference between the two diseases was foundwith the exception of the younger patientsand approximate equality in sex in gastric?ymphoma. Gastroscopically, more展开更多
920380 Primary Non-Hodgkin’s lymphomasof the brain:a clinic-pathological immuno-histochemical study and image analysis of 10cases.I,Immunohistochemical study,LIUKaifeng (刘开凤),et al.Dept Pathol,West-ChinMed Univ.Tu...920380 Primary Non-Hodgkin’s lymphomasof the brain:a clinic-pathological immuno-histochemical study and image analysis of 10cases.I,Immunohistochemical study,LIUKaifeng (刘开凤),et al.Dept Pathol,West-ChinMed Univ.Tumor 1991;11(6):243-245.展开更多
采用皿内测定方法对贵州省石阡县茶树叶部病害Phoma segeticola var. camelliae进行病原生物特性的研究。结果表明,其病原菌可在PDA、OA和MEA培养基上生长,生长速率差异不明显。病原菌适宜在OA培养基上产孢。碳素和氮素营养可影响菌株...采用皿内测定方法对贵州省石阡县茶树叶部病害Phoma segeticola var. camelliae进行病原生物特性的研究。结果表明,其病原菌可在PDA、OA和MEA培养基上生长,生长速率差异不明显。病原菌适宜在OA培养基上产孢。碳素和氮素营养可影响菌株的生长及菌丝色素的形成。25℃是菌株体外适宜生长温度,菌株在中性条件下生长速度最快,但可适应偏酸和偏碱条件。菌株可在PDB培养基中生长,线性生长期大致为36～120 h。展开更多
Four unprecedented sulfur-containing cytochalasans,thiocytochalasins A−D(1−4),were isolated from an endophytic fungus Phoma multirostrata XJ-2-1.Thiocytochalasins A(1)and B(2)feature a novel 5/6/14/5 tetracyclic scaff...Four unprecedented sulfur-containing cytochalasans,thiocytochalasins A−D(1−4),were isolated from an endophytic fungus Phoma multirostrata XJ-2-1.Thiocytochalasins A(1)and B(2)feature a novel 5/6/14/5 tetracyclic scaffold,which are the first examples of cytochalasan containing a thiophene moiety.Thiocytochalasins C(3)and D(4)are epimeric cytochalasan homodimers formed via a thioether bridge.Their structures with absolute configurations were established by detailed analysis of the HRESIMS,NMR,and X-ray crystallography.The plausible biogenetic pathway of 1−4 was postulated.Compounds 3 and 4 exhibited significant cytotoxicity against CT26 cells with IC_(50) values of 0.85 and 0.76µmol/L,respectively.展开更多
文摘A lthough advanced stage aggressive non-Hodgkin'slymphomas and Hodgkin's disease are thought to be che-motherapy-responsive cancers, a considerable number of patients either relapse or never attain a remission. High-dose therapy(HDT) followed by autologous stem cell transplantation(ASCT) is often the only possibility of cure for most of these patients. However, many controversial issues still remain with respect to HDT/ASCT for lymphomas, including its role for, the optimal timing of transplantation, the best conditioning regimen and the potential use of localized radiotherapy or immunologic methods to decrease post-transplant recurrence. Recently, mainly due to the unavailability of carmustine, several novel conditioning protocols have been clinically developed, with the aim of improving the overall outcome by enhancing the anti-lymphoma effect and, at the same time, by reducing short and long-term toxicity. Furthermore, the better safety profiles of novel approaches would definitively allow patients aged more than 65-70 years to benefit from this therapeutic option. In this review, we will briefly discuss the most relevant and recent data available regarding HDT/ASCT in lymphomas.
基金supported by the BMBF(Bundesministerium fr Bildung und Forschung,project no.03F0360A)
文摘Objective:To evaluate the antiniicrobial polenlial of different extracts of the endophytic fungus Phoma sp.and the tentative identification of their active constituents.Methods:The extract and compounds were screened for antimicrobial activity using the Agar Well Diffusion Method.Four compounds were purified using column chromatography and tlieir structures were assigned using～1H and～(13)C NMR spectra,DEPT,2D COSY,HMQC and HMBC experiments.Results:The ethyl acetate fraction of Phoma sp.showed good antifungal,antibacterial,and algicidal properties.One new dihydrofuran derivative,named phomafuranol(1),together with tliree known compounds,phomalacton(2),(3R)-5-hydroxymellein(3)and emodin(4)were isolated from the ethyl acetate fraction of Phoma sp.Preliminary studies indicated that phomalacton(2)displayed strong antibacterial,good antifungal and antialgal activities.Similarly(3R)-5-hydroxymellein(3)and emodin(4)showed good antifungal,antibacterial and algicidal properties.Conclusions:Antimicrobial activities of the ethyl acetate fraction of the endophytic fungus Phoma sp.and isolated compounds clearly demonstrate that Phoma sp.and its active compounds represent a great potential for the food,cosmetic and pharmaceutical industries.
文摘Blackleg disease caused by Phoma lingam is an important disease of oil seed rape (Brassica napus) causing losses up to 95%. The efficacy of microbial antagonists against P. lingam in greenhouse was tested. Serratia plymuthica HRO-C48 and Gliocladium catenulatum J1446 were able to reduce the disease intensity of OSR cotelydones infested with P. lingam at rates 44% and 52% respectively. The reduction of the infestation of the root collar in BBCH14/15 was evaluated as well. Plants treated with a suspension of the antagonists (2 × 105 cfu/plant) and inoculated with either pycnidiospore suspension (2 × 107 cfu/ml) or agar disks grown with P. lingam mycelium, showed a reduced infestation rate of 53% - 93% in the presence of S. plymuthica and 46% - 77% in the presence of G. catenulatum. The efficacy of the antagonist depends highly on their concentration inside OSR seeds. Below 105 cfu/seed no significant difference was recorded between control untreated and infested plants.
文摘Twenty-one cases of primary gastric lym-phoma were compared with gastric cancer inorder to find out their differences in clinicaland endoscopic manifestations. The results showed that little clinical dif-ference between the two diseases was foundwith the exception of the younger patientsand approximate equality in sex in gastric?ymphoma. Gastroscopically, more
文摘920380 Primary Non-Hodgkin’s lymphomasof the brain:a clinic-pathological immuno-histochemical study and image analysis of 10cases.I,Immunohistochemical study,LIUKaifeng (刘开凤),et al.Dept Pathol,West-ChinMed Univ.Tumor 1991;11(6):243-245.
文摘采用皿内测定方法对贵州省石阡县茶树叶部病害Phoma segeticola var. camelliae进行病原生物特性的研究。结果表明,其病原菌可在PDA、OA和MEA培养基上生长,生长速率差异不明显。病原菌适宜在OA培养基上产孢。碳素和氮素营养可影响菌株的生长及菌丝色素的形成。25℃是菌株体外适宜生长温度,菌株在中性条件下生长速度最快,但可适应偏酸和偏碱条件。菌株可在PDB培养基中生长,线性生长期大致为36～120 h。
基金financially supported by the National Natural Science Foundation of China(Nos.22077041 and 31770380).
文摘Four unprecedented sulfur-containing cytochalasans,thiocytochalasins A−D(1−4),were isolated from an endophytic fungus Phoma multirostrata XJ-2-1.Thiocytochalasins A(1)and B(2)feature a novel 5/6/14/5 tetracyclic scaffold,which are the first examples of cytochalasan containing a thiophene moiety.Thiocytochalasins C(3)and D(4)are epimeric cytochalasan homodimers formed via a thioether bridge.Their structures with absolute configurations were established by detailed analysis of the HRESIMS,NMR,and X-ray crystallography.The plausible biogenetic pathway of 1−4 was postulated.Compounds 3 and 4 exhibited significant cytotoxicity against CT26 cells with IC_(50) values of 0.85 and 0.76µmol/L,respectively.
