We utilized Raspberry Pi 4B to develop a microbial monitoring system to simplify the microbial image-capturing process and facilitate the informatization of microbial observation results.The Raspberry Pi 4B firmware,d...We utilized Raspberry Pi 4B to develop a microbial monitoring system to simplify the microbial image-capturing process and facilitate the informatization of microbial observation results.The Raspberry Pi 4B firmware,developed under Python on the Linux platform,achieves sum verification of serial data,file upload based on TCP protocol,control of sequence light source and light valve,real-time self-test based on multithreading,and an experiment-oriented file management method.The system demonstrated improved code logic,scheduling,exception handling,and code readability.展开更多
智慧农业是科技创新与农业的融合。在大数据时代,物联网、人工智能和计算机技术的发展,对传统农业的生产和管理方式进行了改革,推动了农业的信息化和智能化。目前,一个待解决的重要问题是如何运用科技手段实时了解农作物的生长状况。在...智慧农业是科技创新与农业的融合。在大数据时代,物联网、人工智能和计算机技术的发展,对传统农业的生产和管理方式进行了改革,推动了农业的信息化和智能化。目前,一个待解决的重要问题是如何运用科技手段实时了解农作物的生长状况。在此介绍了一个以Raspberry Pi 4b为核心控制节点的智能化农作物生长数据采集处理系统。该系统实现了对农作物远程环境的监控和管理,包括对温度、湿度、光照强度和二氧化碳含量等农作物生长必要影响因素的监测。同时,它还实现了对农作物的科学管理和对环境数据的统计分析。展开更多
目的探讨心肌肥厚性重构可能分子机制。方法选取8周龄清洁级雄性C57小鼠18只,随机分为升主动脉缩窄术(TAC)组和对照组(SHAM组),每组9只。TAC组给予升主动脉缩窄术(TAC术)构建心肌肥厚模型。SHAM组给予假手术。术后4周,利用二氧化碳窒息...目的探讨心肌肥厚性重构可能分子机制。方法选取8周龄清洁级雄性C57小鼠18只,随机分为升主动脉缩窄术(TAC)组和对照组(SHAM组),每组9只。TAC组给予升主动脉缩窄术(TAC术)构建心肌肥厚模型。SHAM组给予假手术。术后4周,利用二氧化碳窒息处死实验小鼠,剖开胸腔后分离心脏组织,用于后续实验。H&E染色观察心脏组织结构;心室/体质量指数评估心室重构情况;荧光测定法测定心脏组织中组蛋白去乙酰化酶(HDACs)活性;qPCR检测MYH7、PDE4b、HDAC4、HDAC5及HDAC9的表达水平,Western blot检测PDE4b及HDAC9蛋白表达水平,ChIP-qPCR检测HDAC9与PDE4b启动子结合水平。结果与SHAM组相比,TAC组小鼠室间隔及左室壁明显增厚,心室/体质量指数明显升高(4.48±0.18 vs 5.50±0.23)(P<0.05),心肌肥厚标志物MYH7表达明显升高(0.91±0.04 vs 1.44±0.07)(P<0.05);PDE4b表达明显降低(mRNA水平0.75±0.09 vs 0.27±0.06;蛋白水平0.67±0.11 vs 0.34±0.05)(P<0.05);肥厚心肌组织中组蛋白去乙酰化酶活性显著增加(3.24±0.32 vs 5.03±0.58)(P<0.05),HDAC9表达显著升高(mRNA水平0.85±0.08 vs 1.35±0.07;蛋白水平0.77±0.10 vs 1.25±0.05)(P<0.05),而HDAC4、HDAC5表达与SHAM组无明显差异(P>0.05);HDAC9与PDE4b启动子结合水平明显升高(1.67±0.10 vs 2.47±0.20)(P<0.05)。结论心肌肥厚中HDAC9可能参与下调PDE4b表达,进而参与心室肌肥厚性重构。展开更多
Objective: To study the effects of TNFAIP3 and PDE4B on apoptosis and invasion of tumor cells in diffuse large B cell lymphoma. Methods: A total of 68 patients who were diagnosed with diffuse large B cell lymphoma in ...Objective: To study the effects of TNFAIP3 and PDE4B on apoptosis and invasion of tumor cells in diffuse large B cell lymphoma. Methods: A total of 68 patients who were diagnosed with diffuse large B cell lymphoma in Guangzhou 421 Hospital of PLA between August 2014 and July 2017 were selected as the DLBCL group of the study, and 34 patients who accepted lymph node biopsy due to lymphadenectasis and were diagnosed with reactive lymphoid hyperplasias in Guangzhou 421 Hospital of PLA during the same period were selected as the control group. The expression levels of TNFAIP3, PDE4B, apoptosis genes and invasion genes in lymph node tissue were determined. Results: TNFAIP3, PTEN, PTPL1 and Bax protein expression in lesion tissue of DLBCL group were significantly lower than those of control group whereas PDE4B, c-myc, AURKA, AURKB, PLK1, Ets-1, β-catenin, MMP7, MMP9 and CD44 protein expression were significantly higher than those of control group;PTEN, PTPL1 and Bax protein expression in DLBCL lesions were positively correlated with TNFAIP3 protein expression and negatively correlated with PDE4B protein expression;c-myc, AURKA, AURKB, PLK1, Ets-1, β-catenin, MMP7, MMP9 and CD44 protein expression were negatively correlated with TNFAIP3 protein expression and positively correlated with PDE4B protein expression. Conclusion: The low expression of TNFAIP3 and the high expression of PDE4B in diffuse large B cell lymphoma can antagonize tumor cell apoptosis and promote tumor cell invasion.展开更多
文摘We utilized Raspberry Pi 4B to develop a microbial monitoring system to simplify the microbial image-capturing process and facilitate the informatization of microbial observation results.The Raspberry Pi 4B firmware,developed under Python on the Linux platform,achieves sum verification of serial data,file upload based on TCP protocol,control of sequence light source and light valve,real-time self-test based on multithreading,and an experiment-oriented file management method.The system demonstrated improved code logic,scheduling,exception handling,and code readability.
文摘智慧农业是科技创新与农业的融合。在大数据时代,物联网、人工智能和计算机技术的发展,对传统农业的生产和管理方式进行了改革,推动了农业的信息化和智能化。目前,一个待解决的重要问题是如何运用科技手段实时了解农作物的生长状况。在此介绍了一个以Raspberry Pi 4b为核心控制节点的智能化农作物生长数据采集处理系统。该系统实现了对农作物远程环境的监控和管理,包括对温度、湿度、光照强度和二氧化碳含量等农作物生长必要影响因素的监测。同时,它还实现了对农作物的科学管理和对环境数据的统计分析。
文摘目的探讨心肌肥厚性重构可能分子机制。方法选取8周龄清洁级雄性C57小鼠18只,随机分为升主动脉缩窄术(TAC)组和对照组(SHAM组),每组9只。TAC组给予升主动脉缩窄术(TAC术)构建心肌肥厚模型。SHAM组给予假手术。术后4周,利用二氧化碳窒息处死实验小鼠,剖开胸腔后分离心脏组织,用于后续实验。H&E染色观察心脏组织结构;心室/体质量指数评估心室重构情况;荧光测定法测定心脏组织中组蛋白去乙酰化酶(HDACs)活性;qPCR检测MYH7、PDE4b、HDAC4、HDAC5及HDAC9的表达水平,Western blot检测PDE4b及HDAC9蛋白表达水平,ChIP-qPCR检测HDAC9与PDE4b启动子结合水平。结果与SHAM组相比,TAC组小鼠室间隔及左室壁明显增厚,心室/体质量指数明显升高(4.48±0.18 vs 5.50±0.23)(P<0.05),心肌肥厚标志物MYH7表达明显升高(0.91±0.04 vs 1.44±0.07)(P<0.05);PDE4b表达明显降低(mRNA水平0.75±0.09 vs 0.27±0.06;蛋白水平0.67±0.11 vs 0.34±0.05)(P<0.05);肥厚心肌组织中组蛋白去乙酰化酶活性显著增加(3.24±0.32 vs 5.03±0.58)(P<0.05),HDAC9表达显著升高(mRNA水平0.85±0.08 vs 1.35±0.07;蛋白水平0.77±0.10 vs 1.25±0.05)(P<0.05),而HDAC4、HDAC5表达与SHAM组无明显差异(P>0.05);HDAC9与PDE4b启动子结合水平明显升高(1.67±0.10 vs 2.47±0.20)(P<0.05)。结论心肌肥厚中HDAC9可能参与下调PDE4b表达,进而参与心室肌肥厚性重构。
基金Projects of Natural Science Foundation of China No:81300397.
文摘Objective: To study the effects of TNFAIP3 and PDE4B on apoptosis and invasion of tumor cells in diffuse large B cell lymphoma. Methods: A total of 68 patients who were diagnosed with diffuse large B cell lymphoma in Guangzhou 421 Hospital of PLA between August 2014 and July 2017 were selected as the DLBCL group of the study, and 34 patients who accepted lymph node biopsy due to lymphadenectasis and were diagnosed with reactive lymphoid hyperplasias in Guangzhou 421 Hospital of PLA during the same period were selected as the control group. The expression levels of TNFAIP3, PDE4B, apoptosis genes and invasion genes in lymph node tissue were determined. Results: TNFAIP3, PTEN, PTPL1 and Bax protein expression in lesion tissue of DLBCL group were significantly lower than those of control group whereas PDE4B, c-myc, AURKA, AURKB, PLK1, Ets-1, β-catenin, MMP7, MMP9 and CD44 protein expression were significantly higher than those of control group;PTEN, PTPL1 and Bax protein expression in DLBCL lesions were positively correlated with TNFAIP3 protein expression and negatively correlated with PDE4B protein expression;c-myc, AURKA, AURKB, PLK1, Ets-1, β-catenin, MMP7, MMP9 and CD44 protein expression were negatively correlated with TNFAIP3 protein expression and positively correlated with PDE4B protein expression. Conclusion: The low expression of TNFAIP3 and the high expression of PDE4B in diffuse large B cell lymphoma can antagonize tumor cell apoptosis and promote tumor cell invasion.