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Prospects of 3D Printing Technology in Dental Medicine
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作者 Ye Sun 《Journal of Clinical and Nursing Research》 2024年第6期398-403,共6页
With the continuous advancement of technology,the application of 3D printing technology in the field of dental medicine is becoming increasingly widespread.This article aims to explore the current applications and fut... With the continuous advancement of technology,the application of 3D printing technology in the field of dental medicine is becoming increasingly widespread.This article aims to explore the current applications and future potential of 3D printing technology in dental medicine and to analyze its benefits and challenges.It first introduces the current state of 3D printing technology in dental implants,crowns,bridges,orthodontics,and maxillofacial surgery.It then discusses the potential applications of 3D printing technology in oral tissue engineering,drug delivery systems,personalized dental prosthetics,and surgical planning.Finally,it analyzes the benefits of 3D printing technology in dental medicine,such as improving treatment accuracy and patient comfort,and shortening treatment times,while also highlighting the challenges faced,such as costs,material choices,and technical limitations.This article aims to provide a reference for professionals in the field of dental medicine and to promote the further application and development of 3D printing technology in this area. 展开更多
关键词 3D printing technology dental medicine dental implants CROWNS Bridges ORTHODONTICS Maxillofacial surgery Tissue engineering Drug delivery systems Personalized dental prosthetics Surgical planning
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Perception of dental appearance and aesthetic analysis among patients,laypersons and dentists 被引量:1
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作者 Alhanoof Aldegheishem Hadeel Mohammed Alfayadh +5 位作者 Munirah AlDossary Shahad Asaad Elzahraa Eldwakhly Nour AL Huda AL Refaei Dana Alsenan Mai Soliman 《World Journal of Clinical Cases》 SCIE 2024年第23期5354-5365,共12页
BACKGROUND Current concepts of beauty are increasingly subjective,influenced by the viewpoints of others.The aim of the study was to evaluate divergences in the perception of dental appearance and smile esthetics amon... BACKGROUND Current concepts of beauty are increasingly subjective,influenced by the viewpoints of others.The aim of the study was to evaluate divergences in the perception of dental appearance and smile esthetics among patients,laypersons and dental practitioners.The study goals were to evaluate the influence of age,sex,education and dental specialty on the participants’judgment and to identify the values of different esthetic criteria.Patients sample included 50 patients who responded to a dental appearance questionnaire(DAQ).Two frontal photographs were taken,one during a smile and one with retracted lips.Laypersons and dentists were asked to evaluate both photographs using a Linear Scale from(0-10),where 0 represent(absolutely unaesthetic)and 10 represent(absolutely aesthetic).One-way analysis of variance(ANOVA)and t-test analysis were measured for each group.Most patients in the sample expressed satisfaction with most aspects of their smiles and dental appearance.Among laypersons(including 488 participants),47 pictures“with lips”out of 50 had higher mean aesthetic scores compared to pictures“without lips”.Among the dentist sample,90 dentists’perception towards the esthetic smile and dental appearance for photos“with lips”and“without lips”were the same for 23 out of 50 patients.Perception of smile aesthetics differed between patients,laypersons and dentists.Several factors can contribute to shape the perception of smile aesthetic.AIM To compare the perception of dental aesthetic among patients,laypersons,and professional dentists,to evaluate the impact of age,sex,educational background,and income on the judgments made by laypersons,to assess the variations in experience,specialty,age,and sex on professional dentists’judgment,and to evaluate the role of lips,skin shade and tooth shade in different participants’judgments.METHODS Patients sample included 50 patients who responded to DAQ.Two frontal photographs were taken:one during a smile and one with retracted lips.Laypersons and dentists were asked to evaluate both photographs using a Linear Scale from(0-10),where 0 represent(absolutely unaesthetic)and 10 represent(absolutely aesthetic).One-way ANOVA and t-test analysis were measured for each group.RESULTS Most patients in the sample expressed satisfaction with most aspects of their smiles and dental appearance.Among laypersons(including 488 participants),47 pictures“with lips”out of 50 had higher mean aesthetic scores compared to pictures“without lips”.Whereas among the dentist sample,90 dentists’perception towards the esthetic smile and dental appearance for photos“with lips”and“without lips”were the same for 23 out of 50 patients.Perception of smile aesthetics differed between patients,laypersons and dentists.CONCLUSION Several factors can contribute to shape the perception of smile aesthetic. 展开更多
关键词 Aesthetic perception Laypersons dental appearance DENTISTS Smile analysis dental aesthetic
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Classification and detection of dental images using meta-learning
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作者 Pradeep Kumar Yadalam Raghavendra Vamsi Anegundi +1 位作者 Mario Alberto Alarcón-Sánchez Artak Heboyan 《World Journal of Clinical Cases》 SCIE 2024年第32期6559-6562,共4页
Meta-learning of dental X-rays is a machine learning technique that can be used to train models to perform new tasks quickly and with minimal input.Instead of just memorizing a task,this is accomplished through teachi... Meta-learning of dental X-rays is a machine learning technique that can be used to train models to perform new tasks quickly and with minimal input.Instead of just memorizing a task,this is accomplished through teaching a model how to learn.Algorithms for meta-learning are typically trained on a collection of training problems,each of which has a limited number of labelled instances.Multiple Xray classification tasks,including the detection of pneumonia,coronavirus disease 2019,and other disorders,have demonstrated the effectiveness of meta-learning.Meta-learning has the benefit of allowing models to be trained on dental X-ray datasets that are too few for more conventional machine learning methods.Due to the high cost and lengthy collection process associated with dental imaging datasets,this is significant for dental X-ray classification jobs.The ability to train models that are more resistant to fresh input is another benefit of meta-learning. 展开更多
关键词 Artificial intelligence META-LEARNING dental diagnosis Image segmentation Medical image interpretation dental radiography
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Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota
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作者 Qing Liu Teng Ma +7 位作者 Cuijiao Feng Yalin Li Hao Jin Xuan Shi Lai-Yu Kwok Yan Shi Tingtao Chen Heping Zhang 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第5期2690-2702,共13页
Conventional filling therapy fails to fundamentally reduce oral cariogenic bacteria.Thus,oral microbiota follow-up intervention after filling would be necessary for improving dental caries prognosis.We recruited 9 car... Conventional filling therapy fails to fundamentally reduce oral cariogenic bacteria.Thus,oral microbiota follow-up intervention after filling would be necessary for improving dental caries prognosis.We recruited 9 caries-free individuals,and 89 dental caries subjects(5 dropouts).Eighty-nine patients were randomized into three groups:caries(n=8;no treatment),control(n=40;filling),and postbiotics(n=41;filling and 14-day Probio-Eco®intervention).Salivary samples were collected at 0 day(after filling)and 14 days.Our results showed that the diversity of dental caries oral microbiota was significantly increased compared with healthy subjects,and filling could restore a healthier oral microbiota partially and temporarily.Thepostbiotics intervention keeps a low alpha-diversity.Co-occurrence network analysis showed that a more stable oral microbiota structure after postbiotics intervention.Taxonomic and functional annotation of the microbiota revealed that postbiotics co-treatment significantly:increased the relative abundance of Pseudomonas and P.reactans,decreased the relative abundance of Prevotella shahii,and enriched the energy metabolism-related pathways.BugBase-predicted phenotypes inferred to an oral microbiota with decreased potential pathogenic bacteria and increased oxidative stress-tolerant bacteria after postbiotics intervention.Collectively,it suggested that postbiotics co-treatment could be a promising strategy that restores the oral microecological balance for dental caries. 展开更多
关键词 Postbiotics Oral microbiota Salivary microbiota dental caries Pacific Biosciences(PacBio)single molecule real-time(SMRT)sequencing dental filling
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Conditioned medium from human dental pulp stem cells treats spinal cord injury by inhibiting microglial pyroptosis 被引量:1
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作者 Tao Liu Ziqian Ma +8 位作者 Liang Liu Yilun Pei Qichao Wu Songjie Xu Yadong Liu Nan Ding Yun Guan Yan Zhang Xueming Chen 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第5期1105-1111,共7页
Human dental pulp stem cell transplantation has been shown to be an effective therapeutic strategy for spinal cord injury.However,whether the human dental pulp stem cell secretome can contribute to functional recovery... Human dental pulp stem cell transplantation has been shown to be an effective therapeutic strategy for spinal cord injury.However,whether the human dental pulp stem cell secretome can contribute to functional recovery after spinal cord injury remains unclear.In the present study,we established a rat model of spinal cord injury based on impact injury from a dropped weight and then intraperitoneally injected the rats with conditioned medium from human dental pulp stem cells.We found that the conditioned medium effectively promoted the recovery of sensory and motor functions in rats with spinal cord injury,decreased expression of the microglial pyroptosis markers NLRP3,GSDMD,caspase-1,and interleukin-1β,promoted axonal and myelin regeneration,and inhibited the formation of glial scars.In addition,in a lipopolysaccharide-induced BV2 microglia model,conditioned medium from human dental pulp stem cells protected cells from pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1βpathway.These results indicate that conditioned medium from human dental pulp stem cells can reduce microglial pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1βpathway,thereby promoting the recovery of neurological function after spinal cord injury.Therefore,conditioned medium from human dental pulp stem cells may become an alternative therapy for spinal cord injury. 展开更多
关键词 BV2 conditioned medium dental pulp stem cells GSDMD MICROGLIA NEUROINFLAMMATION NLRP3 PYROPTOSIS spinal cord injury
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The Dental Absces: Epidemiological, Clinical and Therapeutic Aspects at the Dental Office of the Prefectoral Hospital of Siguiri in 2021 in the Republic of Guinea
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作者 Magna Condé Aly Badara Nabé +6 位作者 Fanta Madi Traoré Moussa Doré Djibrila Camara Kaïn Condé Mory Sacko Francine Manto Kuete Kouamé Patrice Attogbain 《Open Journal of Stomatology》 2024年第9期382-391,共10页
Introduction: A dental abscess is a collection of pus located in the tissues surrounding the tooth roots. The aim of this work is to describe the epidemiological, clinical and therapeutic aspects of dental abscess in ... Introduction: A dental abscess is a collection of pus located in the tissues surrounding the tooth roots. The aim of this work is to describe the epidemiological, clinical and therapeutic aspects of dental abscess in order to contribute to the management of patients suffering from this pathology at the dental office of the Siguiri Prefectural Hospital. Methods: This was a retrospective descriptive study lasting 2 months carried out on the registers of patients received between October 2017 and October 2020 at the dental office of the Siguiri prefectural hospital. Results: 4690 patient files were identified during the study, of which 546 met our selection criteria, i.e. a frequency of 11.64%. During our study, we found 51.83% female compared to 48.17% male, i.e. a sex ratio of 1.07. 54.21% of our patients resided in rural communes compared to 45.76% for the urban commune. The age group most affected was that of 16 - 25 years old or 33.69%. Swelling, pain and functional discomfort were the reasons for consultation in all patients followed by other associated reasons in 41.21%. The premolar-molar group was the most represented, i.e. 86.70%. 80.04% of our patients presented an acute abscess compared to 19.96% of chronic cases. Antibiotic, analgesic, and mouthwash were prescribed in all patients during the study followed by 96.37% of cases prescription of steroidal anti-inflammatory drugs. 93.04% of causal teeth were extracted followed by 6.78% of cases of conservative treatment in our study. In our study, 80.95% of our patients had a good post-therapeutic outcome. Conclusion: This study highlights the need for prevention and early management of dental abscesses. 展开更多
关键词 dental Abscess EPIDEMIOLOGICAL CLINICAL Therapeutic
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Study on the Treatment of Reusable Care Equipment in Public and Private Dental Practices in the City of Conakry
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作者 Magna Condé Aly Badara Nabé +4 位作者 Moussa Doré Julie Gaëlle Yepmo Sambou Alhassane Alpha Camara Amadou Traoré Kouamé Patrice Attogbain 《Open Journal of Stomatology》 2024年第8期351-361,共11页
Introduction: The effective sterilization of reusable instruments in dental care is a crucial issue for public health. The aim of this study was to contribute to improving the processing conditions of reusable care in... Introduction: The effective sterilization of reusable instruments in dental care is a crucial issue for public health. The aim of this study was to contribute to improving the processing conditions of reusable care instruments in dental practices in the city of Conakry. Methods: We carried out an observational and cross-sectional study of a descriptive type lasting three months from January to April 2022 in public and private dental practices approved for activities. Results: A total of 48 dental practices were surveyed, including 7 public and 41 private. 91.67% had the trays for instrument sterilization. 77.08% had a sterilization device. The instruments were: cleaned 100%, disinfected 70.83%, sterilized 20.83% and 20.83% reused the instruments without being sterilized. 72.97% sterilized the instruments at the end of the day. 50% of respondents declared that there were six stages of sterilization. According to standard standards, one dental office or 2.70% respected the normal sterilization process and 13.51% respected the duration and temperature. The storage quality was inadequate at 95.83%. 95.83% of dental practices are at high risk of contamination. The difficulties encountered by staff in daily activity during our study were lack of hygienists (87.50%), insufficient material resources (58.33%), and lack of protocol procedure (45.83%). Conclusion: This study allowed us to note shortcomings of dental surgeons in the process of processing reusable care materials. 展开更多
关键词 TREATMENT MATERIALS CARE REUSABLE dental Practices Conakry
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Prevalence of Dental Anomalies among Patients Who Report to the Komfo Anokye Teaching Hospital’s Orthodontic Clinic
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作者 Ama Agyeibea Amuasi Daniel K. Sabbah +1 位作者 Alexander Oti-Achempong Rahman N. Mamah 《Open Journal of Stomatology》 2024年第2期103-117,共15页
BACKGROUND: Dental anomalies are variations from the established well-known general anatomy and morphology of the tooth as a result of disturbances during tooth formation. They can be developmental, congenital, or acq... BACKGROUND: Dental anomalies are variations from the established well-known general anatomy and morphology of the tooth as a result of disturbances during tooth formation. They can be developmental, congenital, or acquired and may be localized to a single tooth or involve systemic conditions. AIM: To evaluate the prevalence of dental anomalies in patients who report to the Komfo Anokye Teaching Hospital (KATH) dental clinics. METHOD: A descriptive cross-sectional design was used with a sample size of 92 patients aged 18 or older, obtained through convenience sampling. Data analysis was performed using SPSS version 26.0. RESULTS: The study included 92 patients aged 18 to 72 years, with 47.8% males and 52.2% females. Dental anomalies were observed in 51.1% of participants, with a higher prevalence in females (55.3%). The most common anomalies were diastema (48.3%), impacted teeth (22.0%), dilaceration (11.9%), and peg-shaped lateral teeth (6.8%). CONCLUSION: This study highlights the importance of conducting thorough dental examinations to identify and address dental anomalies, which may have implications for treatment. Early detection and correction of these anomalies are crucial to prevent future complications. 展开更多
关键词 dental Anomalies PREVALENCE Impacted Teeth Dilaceration
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Warhead fragments motion trajectories tracking and spatio-temporal distribution reconstruction method based on high-speed stereo photography
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作者 Pengyu Hu Jiangpeng Wu +3 位作者 Zhengang Yan Meng He Chao Liang Hao Bai 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第7期162-172,共11页
High speed photography technique is potentially the most effective way to measure the motion parameter of warhead fragment benefiting from its advantages of high accuracy,high resolution and high efficiency.However,it... High speed photography technique is potentially the most effective way to measure the motion parameter of warhead fragment benefiting from its advantages of high accuracy,high resolution and high efficiency.However,it faces challenge in dense objects tracking and 3D trajectories reconstruction due to the characteristics of small size and dense distribution of fragment swarm.To address these challenges,this work presents a warhead fragments motion trajectories tracking and spatio-temporal distribution reconstruction method based on high-speed stereo photography.Firstly,background difference algorithm is utilized to extract the center and area of each fragment in the image sequence.Subsequently,a multi-object tracking(MOT)algorithm using Kalman filtering and Hungarian optimal assignment is developed to realize real-time and robust trajectories tracking of fragment swarm.To reconstruct 3D motion trajectories,a global stereo trajectories matching strategy is presented,which takes advantages of epipolar constraint and continuity constraint to correctly retrieve stereo correspondence followed by 3D trajectories refinement using polynomial fitting.Finally,the simulation and experimental results demonstrate that the proposed method can accurately track the motion trajectories and reconstruct the spatio-temporal distribution of 1.0×10^(3)fragments in a field of view(FOV)of 3.2 m×2.5 m,and the accuracy of the velocity estimation can achieve 98.6%. 展开更多
关键词 Warhead fragment measurement High speed photography Stereo vision Multi-object tracking Spatio-temporal reconstruction
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Prescription of Antibiotics in Oral-Dental Practice: Case of the Dental Practice at the Coyah Prefectoral Hospital in the Republic of Guinea 2019-2020
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作者 Magna Condé Amadou Traoré +5 位作者 Moussa Doré Aly Badara Nabé David Ugai Seydouba Sylla Mory Doumbouya Alexandre Délamou 《Open Journal of Stomatology》 2024年第9期373-381,共9页
Introduction: Antibiotics are medications that have the property of destroying bacteria or preventing their proliferation. The aim of this study is to evaluate antibiotic prescriptions in oral practice: case of the de... Introduction: Antibiotics are medications that have the property of destroying bacteria or preventing their proliferation. The aim of this study is to evaluate antibiotic prescriptions in oral practice: case of the dental office of the Coyah prefectural hospital. Methods: This is a descriptive cross-sectional study which took place from August 2019 to January 2020 inclusive, i.e. a duration of six (6) months, during which 400 patients were identified. Results: Our results revealed a frequency of 70% of antibiotic prescriptions. The 16 - 25-year-old age group was the most dismayed, at 47.25% and with an average age of 27.78 years. Men represent 55% compared to 45% of women, i.e. sex ratio of 1.22. Pulp gangrene was the most common pathology in 55% of cases. The most prescribed family was Betalactamine or 61.71% and the most represented molecules were Amoxicillin with 56.10% followed by Metronidazole or 34.16%. And the favorable prognosis was observed in 99% of cases. Conclusion: Prescription should always be based on the benefit-risk ratio. This prescription by practitioners aims to prevent or eradicate infections and pain in order to obtain a good therapeutic result. 展开更多
关键词 PRESCRIPTION ANTIBIOTICS Oral Practice dental Office Prefectural Hospital Coyah
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Associated Factors of Dental Caries among Intellectually Disabled Persons in Specialized Centers of Ouagadougou
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作者 Jocelyne Valerie Gare Filwende Dan Kientega +3 位作者 Ouedraogo Carole Nathaie Tenin Ouattara Seydou Kabore Wendpoulomde Aime Désire Nicolas Meda 《Open Journal of Epidemiology》 2024年第2期297-311,共15页
Introduction: Several studies have reported a high prevalence of dental caries among the people with intellectual disabilities. In order to identify the sociodemographic, clinical and behavioral factors associated wit... Introduction: Several studies have reported a high prevalence of dental caries among the people with intellectual disabilities. In order to identify the sociodemographic, clinical and behavioral factors associated with it, we conducted a study in specialized centers welcoming subjects with intellectual disabilities in Ouagadougou (Burkina Faso). Objectives: The objective of this study was to identify the factors associated with it among intellectually disabled in the specialized centers of Ouagadougou. Methods: The study was cross-sectional analytical. The survey took place between November 2020 and January 2021 among people with intellectual disabilities in reception centers receiving a grant from the Ministry of Women, National Solidarity, Family and Humanitarian Action. Data collection was done with a questionnaire adapted from the World Health Organization (WHO) and dental caries was recorded according to WHO criteria in decayed, missing or filled teeth generating a DMF index. Logistic regression was used to identify factors associated with the presence of dental caries. Results: A total of 193 participants were included in the study with an average age of 12.4 years ± 7.9. The overall prevalence of caries measured with the DMF index was 58.6% 95% CI [51.2 - 65.8]. It was significantly associated with female gender (OR = 4.2;95% CI [1.4 - 12.5], p = 0,01), the clinical form of mental illness (epilepsy OR = 3.8 95%;CI [1.2 - 12.7], p = 0,02, trisomy OR = 5.0;95% CI [1.1 - 22.9], p = 0,03, motor autonomy OR = 0.2 95%;CI [0.1 - 0.7], p = 0,01) and at use of toothpaste OR = 9.33;95% CI [1.05 - 82.7], p = 0,04. Conclusion: Dental caries remains a very present pathology in most people living with an intellectual disability. Also, it is necessary to put in place prevention strategies to reduce its prevalence and improve access to oral care for these people. 展开更多
关键词 dental Caries Mental Disability Intellectual Disability Associated Factors OUAGADOUGOU
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An overview of autophagy in the differentiation of dental stem cells
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作者 XITONG ZHAO TIANJUAN JU +3 位作者 XINWEI LI CHANGFENG LIU LULU WANG LI-AN WU 《BIOCELL》 SCIE 2024年第1期47-64,共18页
Dental stem cells(DSCs)have attracted significant interest as autologous stem cells since they are easily accessible and give a minimal immune response.These properties and their ability to both maintain self-renewal ... Dental stem cells(DSCs)have attracted significant interest as autologous stem cells since they are easily accessible and give a minimal immune response.These properties and their ability to both maintain self-renewal and undergo multi-lineage differentiation establish them as key players in regenerative medicine.While many regulatory factors determine the differentiation trajectory of DSCs,prior research has predominantly been based on genetic,epigenetic,and molecular aspects.Recent evidence suggests that DSC differentiation can also be influenced by autophagy,a highly conserved cellular process responsible for maintaining cellular and tissue homeostasis under various stress conditions.This comprehensive review endeavors to elucidate the intricate regulatory mechanism and relationship between autophagy and DSC differentiation.To achieve this goal,we dissect the intricacies of autophagy and its mechanisms.Subsequently,we elucidate its pivotal roles in impacting DSC differentiation,including osteo/odontogenic,neurogenic,and angiogenic trajectories.Furthermore,we reveal the regulatory factors that govern autophagy in DSC lineage commitment,including scaffold materials,pharmaceutical cues,and the extrinsic milieu.The implications of this review are far-reaching,underpinning the potential to wield autophagy as a regulatory tool to expedite DSC-directed differentiation and thereby promote the application of DSCs within the realm of regenerative medicine. 展开更多
关键词 dental stem cells Cell differentiation AUTOPHAGY MITOPHAGY Autophagy regulation
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A Systematic Literature Review of Machine Learning and Deep Learning Approaches for Spectral Image Classification in Agricultural Applications Using Aerial Photography
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作者 Usman Khan Muhammad Khalid Khan +4 位作者 Muhammad Ayub Latif Muhammad Naveed Muhammad Mansoor Alam Salman A.Khan Mazliham Mohd Su’ud 《Computers, Materials & Continua》 SCIE EI 2024年第3期2967-3000,共34页
Recently,there has been a notable surge of interest in scientific research regarding spectral images.The potential of these images to revolutionize the digital photography industry,like aerial photography through Unma... Recently,there has been a notable surge of interest in scientific research regarding spectral images.The potential of these images to revolutionize the digital photography industry,like aerial photography through Unmanned Aerial Vehicles(UAVs),has captured considerable attention.One encouraging aspect is their combination with machine learning and deep learning algorithms,which have demonstrated remarkable outcomes in image classification.As a result of this powerful amalgamation,the adoption of spectral images has experienced exponential growth across various domains,with agriculture being one of the prominent beneficiaries.This paper presents an extensive survey encompassing multispectral and hyperspectral images,focusing on their applications for classification challenges in diverse agricultural areas,including plants,grains,fruits,and vegetables.By meticulously examining primary studies,we delve into the specific agricultural domains where multispectral and hyperspectral images have found practical use.Additionally,our attention is directed towards utilizing machine learning techniques for effectively classifying hyperspectral images within the agricultural context.The findings of our investigation reveal that deep learning and support vector machines have emerged as widely employed methods for hyperspectral image classification in agriculture.Nevertheless,we also shed light on the various issues and limitations of working with spectral images.This comprehensive analysis aims to provide valuable insights into the current state of spectral imaging in agriculture and its potential for future advancements. 展开更多
关键词 Machine learning deep learning unmanned aerial vehicles multi-spectral images image recognition object detection hyperspectral images aerial photography
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RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation
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作者 Daniel B Ferreira Leticia M Gasparoni +1 位作者 Cristiane F Bronzeri Katiucia B S Paiva 《World Journal of Stem Cells》 SCIE 2024年第6期656-669,共14页
BACKGROUND Validation of the reference gene(RG)stability during experimental analyses is essential for correct quantitative real-time polymerase chain reaction(RT-qPCR)data normalisation.Commonly,in an unreliable way,... BACKGROUND Validation of the reference gene(RG)stability during experimental analyses is essential for correct quantitative real-time polymerase chain reaction(RT-qPCR)data normalisation.Commonly,in an unreliable way,several studies use genes involved in essential cellular functions[glyceraldehyde-3-phosphate dehydro-genase(GAPDH),18S rRNA,andβ-actin]without paying attention to whether they are suitable for such experimental conditions or the reason for choosing such genes.Furthermore,such studies use only one gene when Minimum Information for Publication of Quantitative Real-Time PCR Experiments guidelines recom-mend two or more genes.It impacts the credibility of these studies and causes dis-tortions in the gene expression findings.For tissue engineering,the accuracy of gene expression drives the best experimental or therapeutical approaches.We cultivated DPSCs under two conditions:Undifferentiated and osteogenic dif-ferentiation,both for 35 d.We evaluated the gene expression of 10 candidates for RGs[ribosomal protein,large,P0(RPLP0),TATA-binding protein(TBP),GAPDH,actin beta(ACTB),tubulin(TUB),aminolevulinic acid synthase 1(ALAS1),tyro-sine 3-monooxygenase/tryptophan 5-monooxygenase activation protein,zeta(YWHAZ),eukaryotic translational elongation factor 1 alpha(EF1a),succinate dehydrogenase complex,subunit A,flavoprotein(SDHA),and beta-2-micro-globulin(B2M)]every 7 d(1,7,14,21,28,and 35 d)by RT-qPCR.The data were analysed by the four main algorithms,ΔCt method,geNorm,NormFinder,and BestKeeper and ranked by the RefFinder method.We subdivided the samples into eight subgroups.RESULTS All of the data sets from clonogenic and osteogenic samples were analysed using the RefFinder algorithm.The final ranking showed RPLP0/TBP as the two most stable RGs and TUB/B2M as the two least stable RGs.Either theΔCt method or NormFinder analysis showed TBP/RPLP0 as the two most stable genes.However,geNorm analysis showed RPLP0/EF1αin the first place.These algorithms’two least stable RGs were B2M/GAPDH.For BestKeeper,ALAS1 was ranked as the most stable RG,and SDHA as the least stable RG.The pair RPLP0/TBP was detected in most subgroups as the most stable RGs,following the RefFinfer ranking.CONCLUSION For the first time,we show that RPLP0/TBP are the most stable RGs,whereas TUB/B2M are unstable RGs for long-term osteogenic differentiation of human DPSCs in traditional monolayers. 展开更多
关键词 dental pulp stem cells Reference gene Housekeeping gene Endogenous gene Osteogenic differentiation RefFinder
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Hepatocyte growth factor enhances the ability of dental pulp stem cells to ameliorate atherosclerosis in apolipoprotein E-knockout mice
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作者 Han Duan Ning Tao +8 位作者 Lin Lv Kai-Xin Yan Yong-Gang You Zhuang Mao Chang-Yao Wang Xue Li Jia-Yan Jin Chu-Tse Wu Hua Wang 《World Journal of Stem Cells》 SCIE 2024年第5期575-590,共16页
BACKGROUND Atherosclerosis(AS),a chronic inflammatory disease of blood vessels,is a major contributor to cardiovascular disease.Dental pulp stem cells(DPSCs)are capable of exerting immunomodulatory and anti-inflammato... BACKGROUND Atherosclerosis(AS),a chronic inflammatory disease of blood vessels,is a major contributor to cardiovascular disease.Dental pulp stem cells(DPSCs)are capable of exerting immunomodulatory and anti-inflammatory effects by secreting cytokines and exosomes and are widely used to treat autoimmune and inflam-mation-related diseases.Hepatocyte growth factor(HGF)is a pleiotropic cytokine that plays a key role in many inflammatory and autoimmune diseases.AIM To modify DPSCs with HGF(DPSC-HGF)and evaluate the therapeutic effect of DPSC-HGF on AS using an apolipoprotein E-knockout(ApoE-/-)mouse model and an in vitro cellular model.METHODS ApoE-/-mice were fed with a high-fat diet(HFD)for 12 wk and injected with DPSC-HGF or Ad-Null modified DPSCs(DPSC-Null)through tail vein at weeks 4,7,and 11,respectively,and the therapeutic efficacy and mechanisms were analyzed by histopathology,flow cytometry,lipid and glucose measurements,real-time reverse transcription polymerase chain reaction(RT-PCR),and enzyme-linked immunosorbent assay at the different time points of the experiment.An in vitro inflammatory cell model was established by using RAW264.7 cells and human aortic endothelial cells(HAOECs),and indirect co-cultured with supernatant of DPSC-Null(DPSC-Null-CM)or DPSC-HGF-CM,and the effect and mechanisms were analyzed by flow cytometry,RT-PCR and western blot.Nuclear factor-κB(NF-κB)activators and inhibitors were also used to validate the related signaling pathways.RESULTS DPSC-Null and DPSC-HGF treatments decreased the area of atherosclerotic plaques and reduced the expression of inflammatory factors,and the percentage of macrophages in the aorta,and DPSC-HGF treatment had more pronounced effects.DPSCs treatment had no effect on serum lipoprotein levels.The FACS results showed that DPSCs treatment reduced the percentages of monocytes,neutrophils,and M1 macrophages in the peripheral blood and spleen.DPSC-Null-CM and DPSC-HGF-CM reduced adhesion molecule expression in tumor necrosis factor-αstimulated HAOECs and regulated M1 polarization and inflammatory factor expression in lipopolysaccharide-induced RAW264.7 cells by inhibiting the NF-κB signaling pathway.CONCLUSION This study suggested that DPSC-HGF could more effectively ameliorate AS in ApoE-/-mice on a HFD,and could be of greater value in stem cell-based treatments for AS. 展开更多
关键词 ATHEROSCLEROSIS Apolipoprotein E-knockout mice Cell therapy dental pulp stem cells Hepatocyte growth factor
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Human dental pulp stem/stromal cells in clinical practice
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作者 Mohammed E Grawish 《World Journal of Stem Cells》 SCIE 2024年第2期54-57,共4页
Dental pulp stem/stromal cells(DPSCs)are fibroblast-like,neural crest-derived,and multipotent cells that can differentiate into several lineages.They are relatively easy to isolate from healthy and inflamed pulps,with... Dental pulp stem/stromal cells(DPSCs)are fibroblast-like,neural crest-derived,and multipotent cells that can differentiate into several lineages.They are relatively easy to isolate from healthy and inflamed pulps,with little ethical concerns and can be successfully cryopreserved and thawed.The therapeutic effects of DPSCs derived from animal or human sources have been extensively studied through in-vitro and in-vivo animal experiments and the findings indicated that DPSCs are effective not only for dental diseases but also for systemic diseases.Understanding that translational research is a critical step through which the fundamental scientific discoveries could be translated into applicable diagnostics and therapeutics that directly benefit humans,several clinical studies were carried out to generate evidence for the efficacy and safety of autogenous or allogeneic human DPSCs(hDPSCs)as a treatment modality for use in cell-based therapy,regenerative medicine/dentistry and tissue engineering.In clinical medicine,hDPSCs were effective for treating acute ischemic stroke and human exfoliated deciduous teeth-conditioned medium(SHED-CM)repaired vascular damage of the corpus cavernous,which is the main cause of erectile dysfunction.Whereas in clinical dentistry,autologous SHED was able to rege-nerate necrotic dental pulp after implantation into injured teeth,and micrografts enriched with autologous hDPSCs and collagen sponge were considered a treatment option for human intrabony defects.In contrast,hDPSCs did not add a significant regenerative effect when they were used for the treatment of post-extraction sockets.Large-scale clinical studies across diverse populations are still lacking to provide robust evidence on the safety and efficacy of hDPSCs as a new treatment option for various human diseases including dental-related problems. 展开更多
关键词 dental pulp stem/stromal cells Human clinical studies Regenerative medicine Regenerative dentistry Cell-based therapy
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Self-assembly of differentiated dental pulp stem cells facilitates spheroid human dental organoid formation and prevascularization
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作者 Fei Liu Jing Xiao +4 位作者 Lei-Hui Chen Yu-Yue Pan Jun-Zhang Tian Zhi-Ren Zhang Xiao-Chun Bai 《World Journal of Stem Cells》 SCIE 2024年第3期287-304,共18页
BACKGROUND The self-assembly of solid organs from stem cells has the potential to greatly expand the applicability of regenerative medicine.Stem cells can self-organise into microsized organ units,partially modelling ... BACKGROUND The self-assembly of solid organs from stem cells has the potential to greatly expand the applicability of regenerative medicine.Stem cells can self-organise into microsized organ units,partially modelling tissue function and regeneration.Dental pulp organoids have been used to recapitulate the processes of tooth development and related diseases.However,the lack of vasculature limits the utility of dental pulp organoids.AIM To improve survival and aid in recovery after stem cell transplantation,we demonstrated the three-dimensional(3D)self-assembly of adult stem cell-human dental pulp stem cells(hDPSCs)and endothelial cells(ECs)into a novel type of spheroid-shaped dental pulp organoid in vitro under hypoxia and conditioned medium(CM).METHODS During culture,primary hDPSCs were induced to differentiate into ECs by exposing them to a hypoxic environment and CM.The hypoxic pretreated hDPSCs were then mixed with ECs at specific ratios and conditioned in a 3D environment to produce prevascularized dental pulp organoids.The biological characteristics of the organoids were analysed,and the regulatory pathways associated with angiogenesis were studied.RESULTS The combination of these two agents resulted in prevascularized human dental pulp organoids(Vorganoids)that more closely resembled dental pulp tissue in terms of morphology and function.Single-cell RNA sequencing of dental pulp tissue and RNA sequencing of Vorganoids were integrated to analyse key regulatory pathways associated with angiogenesis.The biomarkers forkhead box protein O1 and fibroblast growth factor 2 were identified to be involved in the regulation of Vorganoids.CONCLUSION In this innovative study,we effectively established an in vitro model of Vorganoids and used it to elucidate new mechanisms of angiogenesis during regeneration,facilitating the development of clinical treatment strategies. 展开更多
关键词 Human dental pulp stem cells Prevascularized organoids Integrated analyses ANGIOGENESIS Forkhead box protein O1
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Hepatitis B Vaccination in Medical and Dental Students: A Cross-Sectional Study
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作者 Md. Usama Nasr Md. Kamran Saleem Abdullah Saeed 《Open Journal of Internal Medicine》 2024年第1期122-130,共9页
Background: Medical and dental students are at risk of Hepatitis B Virus (HBV) infection. The study aimed to assess the vaccination status against Hepatitis B Virus of students in clinical and non-clinical years of a ... Background: Medical and dental students are at risk of Hepatitis B Virus (HBV) infection. The study aimed to assess the vaccination status against Hepatitis B Virus of students in clinical and non-clinical years of a private medical and dental college, and their knowledge, attitude, and awareness about the subject. Methods: A cross-sectional study was conducted using a pretested, self-administered questionnaire among 203 medical and dental students of CMH Lahore Medical College and Institute of Dentistry (CMH LMC & IOD) in Lahore, Pakistan. Participants were evaluated for their knowledge and vaccination status against Hepatitis B Virus. Students were considered to be fully vaccinated (recipients of 3 doses), partially vaccinated (recipients of 1 or 2 doses), and unvaccinated. Comparisons were made between students of clinical and non-clinical years. Data was entered and analysed using Statistical Package for the Social Sciences (SPSS) version 23. Results: Only 66% (n = 134) of the 203 participants had ever received a Hepatitis B Virus vaccine out of which a meagre 17.2% (n = 35) were fully vaccinated. No significant difference was found in vaccine uptake between students of clinical and non-clinical years (p-value = 0.181) despite significant differences seen in the knowledge of vaccination schedule (p-value = 0.001), the prevalence of needle-stick injuries (p-value = 0.001), and knowledge of protocols to be followed after a needle-stick injury (p-value = 0.001). Conclusion: Our study found that a large proportion of the student population is vulnerable to HBV infection. There is a need to create awareness regarding the subject to increase vaccine uptake. HBV vaccination should be offered to all currently enrolled students and be made mandatory at the time of admission in the future. 展开更多
关键词 Hepatitis B Vaccine Medical Students dental Students Hepatitis B Vaccination Needle-Stick Injuries
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Comparison of Oral Manifestations in Hospitalized COVID-19 Positive Patients and COVID-19 Negative Dental Outpatients. A Case Series Study and Literature Review
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作者 Flora Zervou-Valvi Emmanouil M. Valvis +5 位作者 Angeliki Giannopoulou Eleana Stoufi Charilaos Samaras Olga Spiropoulou Antonia Hadjicosta Smaragda Diamanti 《International Journal of Otolaryngology and Head & Neck Surgery》 2024年第3期225-250,共26页
Background: The Coronavirus disease 2019 (COVID-19) is a respiratory infectious disease, also named Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which can cause various systemic manifestations that po... Background: The Coronavirus disease 2019 (COVID-19) is a respiratory infectious disease, also named Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which can cause various systemic manifestations that pose a threat to human life. Oral lesions in patients with COVID-19 may appear during or after the illness and may or may not be a consequence of the viral infection. Objective: In this case series we compare the oral manifestations in hospitalized COVID-19 positive patients and COVID-19 negative dental outpatients. Methods: 60 hospitalized COVID-19 patients and 41 control patients, were examined for oral signs and symptoms. The controls were dental patients who visited the hospital for dental care without complaining of any problems related to the oral cavity itself. Results: We have observed a strong association between certain clinical findings and COVID-19, including alterations in taste (ageusia, dysgeusia, and hypogeusia), anosmia, hairy tongue, tongue imprints, red tongue, erythematous candidiasis, pseudomembranous candidiasis, and exfoliative cheilitis. A trend but not statistically significant association at the level of 5% was also noted for colored tongue, linea alba, and pale mucosa. On the contrary, fissured tongue and oral mucosa pigmentation were more frequent in the controls, statistically significant at the level of 5%. Conclusion: COVID-19 has been found to impact the oral cavity, resulting in various oral lesions that can be attributed to either the direct action of the virus or the patient’s immune response. 展开更多
关键词 Case Series COVID-19 SARS-CoV-2 Oral Lesions/Manifestations Hospitalized Patients Control/dental Patients
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Family counseling of dental and oral care for preventing caries of dental during preschool age: a literature review
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作者 Amalia-Dwi Wardani Tantut Susanto Latifa-Aini Susumaningrum 《Nursing Communications》 2024年第9期1-7,共7页
Incorrect family behavior in caring for the dental and oral health of preschool-aged children can affect the incidence of dental caries in these children and can cause more serious health problems in the future if not... Incorrect family behavior in caring for the dental and oral health of preschool-aged children can affect the incidence of dental caries in these children and can cause more serious health problems in the future if not treated immediately,so it is important to find the right solution.This study aims to determine the effect of family counseling on dental and oral care for preschoolers.This study uses a narrative literature review method.Search articles using 5 databases(Pubmed,ScienceDirect,SpringerLink,ProQuest,and Google Scholar)to search for articles with the keywords family counseling AND Oral health AND Early Childhood Caries AND Behavior Change AND Family and the article selection process refers to the Flow diagram of the PRISMA Statement 2020.The results showed that there were 7 articles analyzed in this study.The implementation of family counseling has a significant effect in increasing self-efficacy,knowledge,ability,and motivation of the family,as well as the behavior of the child,to reduce the risk of dental caries in preschool-age children,taking into account several conditions.It was determined that family counseling is effective in reducing the incidence of dental caries in preschool children. 展开更多
关键词 dental caries family counseling oral health care preschool children
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