The Wide Field Survey Telescope(WFST)is a dedicated time-domain multi-band(u,g,r,i,and z)photometric survey facility under construction.In this paper,we present a preliminary study that assesses the quality of photome...The Wide Field Survey Telescope(WFST)is a dedicated time-domain multi-band(u,g,r,i,and z)photometric survey facility under construction.In this paper,we present a preliminary study that assesses the quality of photometric redshifts based on WFST by utilizing mock observations derived with the galaxy catalog in the COSMOS/UltraVISTA field.We apply the template fitting technique to estimate photometric redshifts by using the ZEBRA photometric-redshift code and adopting a modified set of adaptive templates.We evaluate the bias(median relative offset between the output photometric redshifts and input redshifts),normalized median absolute deviation(σ_(NMAD))and outlier fraction(f_(outlier))of photometric redshifts in two typical WFST observational cases,the single 30 s exposure observations(hereafter shallow mode)and co-added 50 minutes exposure observations(hereafter deep mode).We find bias≲0.006,σ_(NMAD)≲0.03,and f_(outlier)≲5%in the shallow mode and bias≈0.005,σ_(NMAD)≈0.06,and f_(outlier)≈17%–27%in the deep mode,respectively,under various lunar phases.Combining the WFST mock observational data with that from the upcoming CSST and Euclid surveys,we demonstrate that the zphot results can be significantly improved,with f_(outlier)≈1%andσ_(NMAD)≈0.02.展开更多
We present a study of star-forming galaxies (SFGs) with pseudobulges (bulges with Sérsic index n<2) in a loca close major-merger galaxy pair sample (H-KPAIR).With data from new aperture photometries in the opt...We present a study of star-forming galaxies (SFGs) with pseudobulges (bulges with Sérsic index n<2) in a loca close major-merger galaxy pair sample (H-KPAIR).With data from new aperture photometries in the optical and near-infrared bands (aperture size of 7 kpc) and from the literature,we find that the mean Age of central stellar populations in Spirals with pseudobulges is consistent with that of disky galaxies and is nearly constant against the bulge-to-total ratio (B/T).Paired Spirals have a slightly lower fraction of pure disk galaxies (B/T≤0.1) than their counterparts in the control sample.Compared to SFGs with classical bulges,those with pseudobulges have a higher (>2σ) mean of specific star formation rate (sSFR) enhancement (sSFR_(enh)=0.33±0.07 versus sSFR_(enh)=0.12±0.06) and broader scatter (by~1 dex).The eight SFGs that have the highest sSFR_(enh)in the sample all have pseudobulges.A majority (69%) of paired SFGs with strong enhancement (having sSFR more than5 times the median of the control galaxies) have pseudobulges.The Spitzer data show that the pseudobulges in these galaxies are tightly linked to nuclear/circum-nuclear starbursts.Pseudobulge SFGs in S+S and in S+E pairs have significantly (>3σ) different sSFR enhancement,with the means of sSFR_(enh)=0.45±0.08 and-0.04±0.11respectively.We find a decrease in the sSFR enhancements with the density of the environment for SFGs with pseudobulges.Since a high fraction (5/11) of pseudobulge SFGs in S+E pairs are in rich groups/clusters (loca density N_(1Mpc)≥7),the dense environment might be the cause for their low s SFR_(enh).展开更多
在冰乙酸介质中,利用亚硝酸根催化条件下,溴酸钾氧化茜素红的褪色反应,建立催化动力学光度法,对痕量NO_(2)^(-)进行测定.通过单因素实验得到了各种成分的加入量、反应温度和反应时间等最佳条件.结果发现,在反应体系中,茜素红浓度与NO_(2...在冰乙酸介质中,利用亚硝酸根催化条件下,溴酸钾氧化茜素红的褪色反应,建立催化动力学光度法,对痕量NO_(2)^(-)进行测定.通过单因素实验得到了各种成分的加入量、反应温度和反应时间等最佳条件.结果发现,在反应体系中,茜素红浓度与NO_(2)^(-)含量之间线性关系良好,在0.02~0.14μg/mL范围内,线性回归方程为ln(A_(0)/A)=1.43 c NO_(3)^(-)(μg/mL)+0.00859,相关系数r^(2)为0.9970,检出限为5.25×10^(-3)μg/mL.该方法操作简单、稳定性好.展开更多
The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain functio...The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with emotions.Specifically, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal mice.After knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was obviously mitigated. Additionally, administration of an excitatory amino acid transporter 2 inhibitor in the basolateral amygdala yielded a notable reduction in anxiety level among mice subjected to stress. These results suggest that basolateral amygdala astrocytic excitatory amino acid transporter 2 plays a role in in the regulation of unpredictable chronic mild stress-induced anxiety-like behavior by impacting the activity of local glutamatergic neurons, and targeting excitatory amino acid transporter 2 in the basolateral amygdala holds therapeutic promise for addressing anxiety disorders.展开更多
The 85-cm telescope at Xinglong station is a prime focus system that operates well with high science outputs. The telescope has been upgraded since 2014 with a new corrector, and new filters and camera, which are prov...The 85-cm telescope at Xinglong station is a prime focus system that operates well with high science outputs. The telescope has been upgraded since 2014 with a new corrector, and new filters and camera, which are provided by Beijing Normal University. The filter set is the Johnson-Cousins UBV RI system. We report the test results of the new system including bias, dark current, linearity, gain and readout noise of the CCD camera. Then we derive accurate instrumental calibration coefficients in UBV RI bands with Landolt standard stars during photometric nights. Finally, we give the limiting magnitudes with various exposure times and signal-to-noise ratios for observers as references.展开更多
Aim: The elemental composition of the epididymal luminal fluid (ELF) in adult goat ( Capra indica) was investigat-ed. Methods: ELF was collected by micropuncture from twelve sites along the epididymal duct. The elemen...Aim: The elemental composition of the epididymal luminal fluid (ELF) in adult goat ( Capra indica) was investigat-ed. Methods: ELF was collected by micropuncture from twelve sites along the epididymal duct. The elementalcontents was analyzed with inductively coupled plasma (ICP) emission spectroscopy, a microanalytical technique thatcan simultaneously measure many elements in minute volumes of sample. The Na and K concentrations were determinedby flame photometry. Results: ICP spectroscopy showed the presence of copper, calcium, nickel, iron, magne-sium, chromium, titanium and zinc in ELF, with fluctuating levels at different sites along the length of the epididymis.Cadmium, cobalt, lead and manganese were not fotmd. The Na^+ / K^+ ratio was seen to be higher at the initial seg-ments of the epididymis and lower at the distal. Conclusion: It is proposed that the observed characteristic distribu-tion of elements in ELF may have far reaching implications in sperm maturation and storage known to occur in the epi-didymis. (Asian J Androl 2000 Dec; 2: 288-292)展开更多
The Nanshan One-meter Wide-field Telescope is a prime focus system that is located at Nanshan Station of Xinjiang Astronomical Observatories.The field of view was designed to 1.5°× 1.5°,and the Johnson-...The Nanshan One-meter Wide-field Telescope is a prime focus system that is located at Nanshan Station of Xinjiang Astronomical Observatories.The field of view was designed to 1.5°× 1.5°,and the Johnson-Cousins UBVRI system was chosen as the main filter set.The telescope has been providing observation services for astronomers since September 2013.Variable source searching and time-domain surveys are the main scientific goals.The system’s test results are reported including linearity,dark current,bias,readout noise and gain of the CCD camera.The accurate instrumental calibration coefficients in UBVRI bands were driven with Landolt standard stars during photometric nights.Finally,the limiting magnitudes are given with signal-to-noise ratios and various exposure times for observers.展开更多
We present a progress of a long term project to obtain UBVI CCD photometry of open clusters which were little studied before, using the Doyak 1.8 m telecope of Bohyunsan Optical Astronomy Observatory operated by the K...We present a progress of a long term project to obtain UBVI CCD photometry of open clusters which were little studied before, using the Doyak 1.8 m telecope of Bohyunsan Optical Astronomy Observatory operated by the Korea Astronomical Observatory, in Korea. The primary goals of this project are (1) to make a catalog of UBVI photometry of open clusters, (2) to make an atlas of open clusters, and (3) to survey and monitor variable stars in open clusters.展开更多
Evidence for the long-term optical curve variability and colour behaviour of the BL Lac object Mkn 421 is presented. Our results show that the amplitude of the optical variations of Mkn 421 is only about △B=4.7 magni...Evidence for the long-term optical curve variability and colour behaviour of the BL Lac object Mkn 421 is presented. Our results show that the amplitude of the optical variations of Mkn 421 is only about △B=4.7 magnitude in the B-band for its photometric history from 1899 to 2002. The results of optical photometric monitoring of the Mkn 421 from April 2000 to Jan 2002 are provided. During our observation, Mkn 421 shows significant rapid variations and exhibits short time variability of 2.42 hours in the B band. A strong correlation between the B - V colour index and the magnitude in the B band is found. Our charge coupled device (CCD) photometry of Mkn 421 shows that the measured results are in good agreement with the predicted optical variability period of about 23 years.展开更多
Manipulating and real-time monitoring of neuronal activities with cell-type specificity and precise spatiotemporal resolution during animal behavior are fundamental technologies for exploring the functional connectivi...Manipulating and real-time monitoring of neuronal activities with cell-type specificity and precise spatiotemporal resolution during animal behavior are fundamental technologies for exploring the functional connectivity, information transmission, and physiological functions of neural circuits in vivo. However, current techniques for optogenetic stimulation and neuronal activity recording mostly operate independently. Here, we report an all-fiber-transmission photometry system for simultaneous optogenetic manipulation and multi-color recording of neuronal activities and the neurotransmitter release in a freely moving animal. We have designed and manufactured a wavelength-independent multi-branch fiber bundle to enable simultaneous optogenetic manipulation and multi-color recording at different wavelengths. Further, we combine a laser of narrow linewidth with the lock-in amplification method to suppress the optogenetic stimulation-induced artifacts and channel crosstalk. We show that the collection efficiency of our system outperforms a traditional epi-fluorescence system. Further, we demonstrate successful recording of dynamic dopamine(DA) responses to unexpected rewards in the nucleus accumbens(NAc) in a freely moving mouse. We also show simultaneous dual-color recording of neuronal Ca2+ signals and DA dynamics in the NAc upon delivering an unexpected reward and the simultaneous optogenetic activating at dopaminergic terminals in the same location. Thus, our multi-function fiber photometry system provides a compatible, efficient, and flexible solution for neuroscientists to study neural circuits and neurological diseases.展开更多
In vito fber photometry is a powerful technique to analyze the dy namics of population neurons during fiunctional study of neuroscience.Here,we introduced a detailed protocol for fiber photometry-based calciun reordin...In vito fber photometry is a powerful technique to analyze the dy namics of population neurons during fiunctional study of neuroscience.Here,we introduced a detailed protocol for fiber photometry-based calciun reording in freely moving mice,covering from virus injection,fiber stub insertion,optogenetical stimulation to data procurement and analysis.Furthemnore,we applied this protocol to explore neuronal activity of mice latenal-posterior(LP)thalaric nucleus in response to optogenetical stimulation of primary visual cortex(V1)neurons,and explore axon clusters activity of optogenetically evoked V1 neurons.Final confirmation of virus-based protein expression in V1 and precise fber insertion indicated that the surgery procedure of this protocol is reliable for functional calcium recording.The scripts for data analysis and some tips in our protocol are provided in details.Together,this protocol is simple,low-cost,and effective for neuronal activity detection by fiber photometry,which will hep neuroscience researchers to carry out fiunctional and behavioral study in vivo.展开更多
We will introduce the construction and design of a new simultaneous three-channel multicolor CCD photometer.This photometer has been mounted on the 1.2 m telescope at Jilin Astronomical Observatory,and is applied to s...We will introduce the construction and design of a new simultaneous three-channel multicolor CCD photometer.This photometer has been mounted on the 1.2 m telescope at Jilin Astronomical Observatory,and is applied to study space debris,gamma-ray burst afterglows,asteroids and other rapidly variable objects.As one of the ground follow-up telescopes of the Chinese-French Space-based multi-band astronomical Variable Objects Monitor(SVOM)mission,it appears very essential to evaluate the performance of the photometry system of this photometer,which can achieve simultaneous imaging within a field of view of 21’.5×21’.5,21’.5×21’.5 and21’.3×21’.3 in the Sloan Digital Sky Survey(SDSS)g’,r’,i’bands,respectively.Photometric calibrations were carried out by using plenty of SDSS standard stars,and the relationship between the photometric system and the Johnson-Bessel filter system was also studied.The results of the performance evaluation from observing open cluster M67 are presented.展开更多
After comparing the influences of each rare earth(Ⅲ),11 species of metal(Ⅱ)and 8 species of acidic triphenylmethane dye on the formation of coloured coordination compound in the system of Metal(Ⅱ)-RE (Ⅲ)-Dye,it wa...After comparing the influences of each rare earth(Ⅲ),11 species of metal(Ⅱ)and 8 species of acidic triphenylmethane dye on the formation of coloured coordination compound in the system of Metal(Ⅱ)-RE (Ⅲ)-Dye,it was proven that the co-colouration effect can be caused only by the xanthene dyes(XD)con- taining substituting hydroxy.group on the ortho-position of etheric oxygen group.Besides,the radius of met- al(Ⅱ)must not be smaller than a definite limit;otherwise,no co-colouration appears.Furthermore,the acid- ity of medium is another important factor to affect co-colouration,Bromopyrogallol red causes co-colouration of Pb(Ⅱ)-RE(Ⅲ)only,owing to its higher chromophoric acidity;while alizarin violet causes co-colouration of M(Ⅱ)-RE(Ⅲ)(M=Pb,Ca,Sr and Ba),due to its lower chromophoric acidity.The condi- tions causing co-colouration,the regularities of co-colouration and the influences of co-colouration in photometric determination of metals were discussed.展开更多
Extraction spectrophotometric determination of dicyanoaurate (Ⅰ) with Crystal Violet is described. At pH 2.6~10.7, dicyanoaurate (Ⅰ) can react with Crystal Violet and form a complex which can be extracted into iso...Extraction spectrophotometric determination of dicyanoaurate (Ⅰ) with Crystal Violet is described. At pH 2.6~10.7, dicyanoaurate (Ⅰ) can react with Crystal Violet and form a complex which can be extracted into isoamylacetate. The apparent molar absorptivity is 8.37×10 4 L·mol -1 ·cm -1 at 605 nm. Beer′s law is obeyed for 0~15 μg of Au(Ⅰ) per 5 ml of isoamylacetate. The interference from Ag(Ⅰ) and Cu(Ⅱ) can be eliminated with thiourea and EDTA. The method is applied to the determination of dicyanoaurate (Ⅰ) in sample solution with satisfactory results.展开更多
基金supported by the National Key R&D Program of China(2022YFF0503401)the National Natural Science Foundation of China(12203047,12025303,11890693,12003031)+1 种基金the science research grants from the China Manned Space Project with NO.CMSCSST-2021-A06the Fundamental Research Funds for the Central Universities(WK3440000006,WK2030000057)。
文摘The Wide Field Survey Telescope(WFST)is a dedicated time-domain multi-band(u,g,r,i,and z)photometric survey facility under construction.In this paper,we present a preliminary study that assesses the quality of photometric redshifts based on WFST by utilizing mock observations derived with the galaxy catalog in the COSMOS/UltraVISTA field.We apply the template fitting technique to estimate photometric redshifts by using the ZEBRA photometric-redshift code and adopting a modified set of adaptive templates.We evaluate the bias(median relative offset between the output photometric redshifts and input redshifts),normalized median absolute deviation(σ_(NMAD))and outlier fraction(f_(outlier))of photometric redshifts in two typical WFST observational cases,the single 30 s exposure observations(hereafter shallow mode)and co-added 50 minutes exposure observations(hereafter deep mode).We find bias≲0.006,σ_(NMAD)≲0.03,and f_(outlier)≲5%in the shallow mode and bias≈0.005,σ_(NMAD)≈0.06,and f_(outlier)≈17%–27%in the deep mode,respectively,under various lunar phases.Combining the WFST mock observational data with that from the upcoming CSST and Euclid surveys,we demonstrate that the zphot results can be significantly improved,with f_(outlier)≈1%andσ_(NMAD)≈0.02.
基金supported by the National Natural Science Foundation of China(NSFC)No.11873055 and No.11933003sponsored(in part)by the Chinese Academy of Sciences(CAS)through a grant to the CAS South America Center for Astronomy(CASSACA)+4 种基金support from project PID2020-114414GB-100,financed by MCIN/AEI/10.13039/501100011033the Junta de Andaluciaía(Spain)grant FQM108support by the National Key R&D Program of China No.2017YFA0402600the National Natural Science Foundation of China(NSFC)grant Nos.11890692,12133008,and 12221003China Manned Space Project No.CMS-CSST2021-A04。
文摘We present a study of star-forming galaxies (SFGs) with pseudobulges (bulges with Sérsic index n<2) in a loca close major-merger galaxy pair sample (H-KPAIR).With data from new aperture photometries in the optical and near-infrared bands (aperture size of 7 kpc) and from the literature,we find that the mean Age of central stellar populations in Spirals with pseudobulges is consistent with that of disky galaxies and is nearly constant against the bulge-to-total ratio (B/T).Paired Spirals have a slightly lower fraction of pure disk galaxies (B/T≤0.1) than their counterparts in the control sample.Compared to SFGs with classical bulges,those with pseudobulges have a higher (>2σ) mean of specific star formation rate (sSFR) enhancement (sSFR_(enh)=0.33±0.07 versus sSFR_(enh)=0.12±0.06) and broader scatter (by~1 dex).The eight SFGs that have the highest sSFR_(enh)in the sample all have pseudobulges.A majority (69%) of paired SFGs with strong enhancement (having sSFR more than5 times the median of the control galaxies) have pseudobulges.The Spitzer data show that the pseudobulges in these galaxies are tightly linked to nuclear/circum-nuclear starbursts.Pseudobulge SFGs in S+S and in S+E pairs have significantly (>3σ) different sSFR enhancement,with the means of sSFR_(enh)=0.45±0.08 and-0.04±0.11respectively.We find a decrease in the sSFR enhancements with the density of the environment for SFGs with pseudobulges.Since a high fraction (5/11) of pseudobulge SFGs in S+E pairs are in rich groups/clusters (loca density N_(1Mpc)≥7),the dense environment might be the cause for their low s SFR_(enh).
文摘在冰乙酸介质中,利用亚硝酸根催化条件下,溴酸钾氧化茜素红的褪色反应,建立催化动力学光度法,对痕量NO_(2)^(-)进行测定.通过单因素实验得到了各种成分的加入量、反应温度和反应时间等最佳条件.结果发现,在反应体系中,茜素红浓度与NO_(2)^(-)含量之间线性关系良好,在0.02~0.14μg/mL范围内,线性回归方程为ln(A_(0)/A)=1.43 c NO_(3)^(-)(μg/mL)+0.00859,相关系数r^(2)为0.9970,检出限为5.25×10^(-3)μg/mL.该方法操作简单、稳定性好.
基金supported by the National Natural Science Foundation of China,Nos.32371070 (to JT),31761163005 (to JT),32100824 (to QX)the Shenzhen Science and Technology Program,Nos.RCBS20210609104606024 (to QX),JCY20210324101813035 (to DL)+4 种基金the Guangdong Provincial Key S&T Program,No.2018B030336001 (to JT)the Key Basic Research Program of Shenzhen Science and Technology Innovation Commission,Nos.JCYJ20200109115405930 (to JT),JCYJ20220818101615033 (to DL),JCYJ20210324115811031 (to QX),JCYJ20200109150717745 (to QX)Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological Diseases,No.ZDSYS20220304163558001 (to JT)Guangdong Provincial Key Laboratory of Brain Connectome and Behavior,No.2023B1212060055 (to JT)the China Postdoctoral Science Foundation,No.2021M693298 (to QX)。
文摘The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with emotions.Specifically, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal mice.After knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was obviously mitigated. Additionally, administration of an excitatory amino acid transporter 2 inhibitor in the basolateral amygdala yielded a notable reduction in anxiety level among mice subjected to stress. These results suggest that basolateral amygdala astrocytic excitatory amino acid transporter 2 plays a role in in the regulation of unpredictable chronic mild stress-induced anxiety-like behavior by impacting the activity of local glutamatergic neurons, and targeting excitatory amino acid transporter 2 in the basolateral amygdala holds therapeutic promise for addressing anxiety disorders.
基金support from the staff at the Xinglong 85-cm telescopesupport from the National Natural Science Foundation of China(NSFC)through grant 11673003+3 种基金the National Basic Research Program of China(973 program,2014CB845700)partially supported by the Open Project Program of the Key Laboratory of Optical Astronomy(National Astronomical Observatories)NSFC under Grant Nos.11403088 and 11273051(Xinjiang Astronomical Observatory)Chinese Academy of Sciences and Department of Astronomy,Beijing Normal University
文摘The 85-cm telescope at Xinglong station is a prime focus system that operates well with high science outputs. The telescope has been upgraded since 2014 with a new corrector, and new filters and camera, which are provided by Beijing Normal University. The filter set is the Johnson-Cousins UBV RI system. We report the test results of the new system including bias, dark current, linearity, gain and readout noise of the CCD camera. Then we derive accurate instrumental calibration coefficients in UBV RI bands with Landolt standard stars during photometric nights. Finally, we give the limiting magnitudes with various exposure times and signal-to-noise ratios for observers as references.
文摘Aim: The elemental composition of the epididymal luminal fluid (ELF) in adult goat ( Capra indica) was investigat-ed. Methods: ELF was collected by micropuncture from twelve sites along the epididymal duct. The elementalcontents was analyzed with inductively coupled plasma (ICP) emission spectroscopy, a microanalytical technique thatcan simultaneously measure many elements in minute volumes of sample. The Na and K concentrations were determinedby flame photometry. Results: ICP spectroscopy showed the presence of copper, calcium, nickel, iron, magne-sium, chromium, titanium and zinc in ELF, with fluctuating levels at different sites along the length of the epididymis.Cadmium, cobalt, lead and manganese were not fotmd. The Na^+ / K^+ ratio was seen to be higher at the initial seg-ments of the epididymis and lower at the distal. Conclusion: It is proposed that the observed characteristic distribu-tion of elements in ELF may have far reaching implications in sperm maturation and storage known to occur in the epi-didymis. (Asian J Androl 2000 Dec; 2: 288-292)
基金supported by the program of the light in China’s Western Region(LCWRGrant Nos.2015-XBQN-B-04,2015-XBQN-A02)+5 种基金the National Natural Science Foundation of China(Grant Nos.11803076,11873081,11661161016 and U1831209)the 13th Five-year Informatization Plan of Chinese Academy of Sciences(Grant No.XXH13503–03–107)the Youth Innovation Promotion Association CAS(Grant Nos.2014050,2018080)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB23040100)2017 Heaven Lake Hundred-Talent Program of Xinjiang Uygur Autonomous Region of Chinathe Open Project Program of the Key Laboratory of Optical Astronomy(NAOC)。
文摘The Nanshan One-meter Wide-field Telescope is a prime focus system that is located at Nanshan Station of Xinjiang Astronomical Observatories.The field of view was designed to 1.5°× 1.5°,and the Johnson-Cousins UBVRI system was chosen as the main filter set.The telescope has been providing observation services for astronomers since September 2013.Variable source searching and time-domain surveys are the main scientific goals.The system’s test results are reported including linearity,dark current,bias,readout noise and gain of the CCD camera.The accurate instrumental calibration coefficients in UBVRI bands were driven with Landolt standard stars during photometric nights.Finally,the limiting magnitudes are given with signal-to-noise ratios and various exposure times for observers.
文摘We present a progress of a long term project to obtain UBVI CCD photometry of open clusters which were little studied before, using the Doyak 1.8 m telecope of Bohyunsan Optical Astronomy Observatory operated by the Korea Astronomical Observatory, in Korea. The primary goals of this project are (1) to make a catalog of UBVI photometry of open clusters, (2) to make an atlas of open clusters, and (3) to survey and monitor variable stars in open clusters.
基金Project supported by an CRCG grant of the University of Hong Kong, Yunnan Provincial Foundation of Science of China (Grant No 2004A0027M) and the National Natural Science Foundation of China(Grant No 10363002), and the Natural Science Foundation of China Education Department(Grant No 02142).
文摘Evidence for the long-term optical curve variability and colour behaviour of the BL Lac object Mkn 421 is presented. Our results show that the amplitude of the optical variations of Mkn 421 is only about △B=4.7 magnitude in the B-band for its photometric history from 1899 to 2002. The results of optical photometric monitoring of the Mkn 421 from April 2000 to Jan 2002 are provided. During our observation, Mkn 421 shows significant rapid variations and exhibits short time variability of 2.42 hours in the B band. A strong correlation between the B - V colour index and the magnitude in the B band is found. Our charge coupled device (CCD) photometry of Mkn 421 shows that the measured results are in good agreement with the predicted optical variability period of about 23 years.
基金supported by Beijing Municipal Governmentsupported by the National Natural Science Foundation of China(Grant Nos.61890952)the Director Fund of WNLO。
文摘Manipulating and real-time monitoring of neuronal activities with cell-type specificity and precise spatiotemporal resolution during animal behavior are fundamental technologies for exploring the functional connectivity, information transmission, and physiological functions of neural circuits in vivo. However, current techniques for optogenetic stimulation and neuronal activity recording mostly operate independently. Here, we report an all-fiber-transmission photometry system for simultaneous optogenetic manipulation and multi-color recording of neuronal activities and the neurotransmitter release in a freely moving animal. We have designed and manufactured a wavelength-independent multi-branch fiber bundle to enable simultaneous optogenetic manipulation and multi-color recording at different wavelengths. Further, we combine a laser of narrow linewidth with the lock-in amplification method to suppress the optogenetic stimulation-induced artifacts and channel crosstalk. We show that the collection efficiency of our system outperforms a traditional epi-fluorescence system. Further, we demonstrate successful recording of dynamic dopamine(DA) responses to unexpected rewards in the nucleus accumbens(NAc) in a freely moving mouse. We also show simultaneous dual-color recording of neuronal Ca2+ signals and DA dynamics in the NAc upon delivering an unexpected reward and the simultaneous optogenetic activating at dopaminergic terminals in the same location. Thus, our multi-function fiber photometry system provides a compatible, efficient, and flexible solution for neuroscientists to study neural circuits and neurological diseases.
基金supported by the National Natural Science Foundation of China (Grant Nos.31371106 and 91632110)HZAU Independent Innovation Fund (2014BQ019).
文摘In vito fber photometry is a powerful technique to analyze the dy namics of population neurons during fiunctional study of neuroscience.Here,we introduced a detailed protocol for fiber photometry-based calciun reording in freely moving mice,covering from virus injection,fiber stub insertion,optogenetical stimulation to data procurement and analysis.Furthemnore,we applied this protocol to explore neuronal activity of mice latenal-posterior(LP)thalaric nucleus in response to optogenetical stimulation of primary visual cortex(V1)neurons,and explore axon clusters activity of optogenetically evoked V1 neurons.Final confirmation of virus-based protein expression in V1 and precise fber insertion indicated that the surgery procedure of this protocol is reliable for functional calcium recording.The scripts for data analysis and some tips in our protocol are provided in details.Together,this protocol is simple,low-cost,and effective for neuronal activity detection by fiber photometry,which will hep neuroscience researchers to carry out fiunctional and behavioral study in vivo.
基金Supported by the National Natural Science Foundation of China。
文摘We will introduce the construction and design of a new simultaneous three-channel multicolor CCD photometer.This photometer has been mounted on the 1.2 m telescope at Jilin Astronomical Observatory,and is applied to study space debris,gamma-ray burst afterglows,asteroids and other rapidly variable objects.As one of the ground follow-up telescopes of the Chinese-French Space-based multi-band astronomical Variable Objects Monitor(SVOM)mission,it appears very essential to evaluate the performance of the photometry system of this photometer,which can achieve simultaneous imaging within a field of view of 21’.5×21’.5,21’.5×21’.5 and21’.3×21’.3 in the Sloan Digital Sky Survey(SDSS)g’,r’,i’bands,respectively.Photometric calibrations were carried out by using plenty of SDSS standard stars,and the relationship between the photometric system and the Johnson-Bessel filter system was also studied.The results of the performance evaluation from observing open cluster M67 are presented.
文摘After comparing the influences of each rare earth(Ⅲ),11 species of metal(Ⅱ)and 8 species of acidic triphenylmethane dye on the formation of coloured coordination compound in the system of Metal(Ⅱ)-RE (Ⅲ)-Dye,it was proven that the co-colouration effect can be caused only by the xanthene dyes(XD)con- taining substituting hydroxy.group on the ortho-position of etheric oxygen group.Besides,the radius of met- al(Ⅱ)must not be smaller than a definite limit;otherwise,no co-colouration appears.Furthermore,the acid- ity of medium is another important factor to affect co-colouration,Bromopyrogallol red causes co-colouration of Pb(Ⅱ)-RE(Ⅲ)only,owing to its higher chromophoric acidity;while alizarin violet causes co-colouration of M(Ⅱ)-RE(Ⅲ)(M=Pb,Ca,Sr and Ba),due to its lower chromophoric acidity.The condi- tions causing co-colouration,the regularities of co-colouration and the influences of co-colouration in photometric determination of metals were discussed.
文摘Extraction spectrophotometric determination of dicyanoaurate (Ⅰ) with Crystal Violet is described. At pH 2.6~10.7, dicyanoaurate (Ⅰ) can react with Crystal Violet and form a complex which can be extracted into isoamylacetate. The apparent molar absorptivity is 8.37×10 4 L·mol -1 ·cm -1 at 605 nm. Beer′s law is obeyed for 0~15 μg of Au(Ⅰ) per 5 ml of isoamylacetate. The interference from Ag(Ⅰ) and Cu(Ⅱ) can be eliminated with thiourea and EDTA. The method is applied to the determination of dicyanoaurate (Ⅰ) in sample solution with satisfactory results.