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Genetic Analysis and Primary Mapping of pms4, a Photoperiod-Sensitive Genic Male Sterility Gene in Rice (Oryza sativa) 被引量:13
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作者 HUANG Ting-you WANG Zhi HU Yun-gao SHI Shou-pei PENG Tao CHU Xu-dong SHI Jun XIANG Zu-fen LIU Ding-you 《Rice science》 SCIE 2008年第2期153-156,共4页
To understand the genetic characteristics of a new photoperiod-sensitive genic male sterile line Mian 9S, some reciprocal crosses were made between Mian 9S and six indica rice materials, Yangdao 6, Luhui 602, Shuihui ... To understand the genetic characteristics of a new photoperiod-sensitive genic male sterile line Mian 9S, some reciprocal crosses were made between Mian 9S and six indica rice materials, Yangdao 6, Luhui 602, Shuihui 527, Mianhui 725, Fuhui 838 and Yixiang 1B. Genetic analysis results suggested that the photoperiod-sensitive genic male sterility (PGMS) of Mian 9S was controlled by a single recessive nuclear gene. Thus, the F2 population derived from the cross of Yangdao 6/Mian 9S was used to map the PGMS gene in Mian 9S. By using SSR markers, the PGMS gene of Mian 9S was mapped on one side of the markers, RM6659 and RM1305, on rice chromosome 4, with the genetic distances of 3.0 cM and 3.5 cM, respectively. The gene was a novel PGMS gene and designated tentatively as pms4. In addition, the application of the pms4 gene was discussed. 展开更多
关键词 genetic analysis gene mapping photoperiod-sensitive genic male sterility rice (Oryza sativa)
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Genetics of Fertility Restoration in Cytoplasmic Male Sterile Pepper 被引量:3
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作者 WANG Li-hao ZHANG Bao-xi +5 位作者 AM Daubeze HUANG San-wen GUO Jia-zhen MAO Sheng-li A Palloix DU Yong-chen 《Agricultural Sciences in China》 CAS CSCD 2006年第3期188-195,共8页
Pepper hybrid seeds production using male sterility could lower cost by reducing time and labour, and increase the genetic purity of the F1 seeds. To investigate the genetics of fertility restoration of the Peterson c... Pepper hybrid seeds production using male sterility could lower cost by reducing time and labour, and increase the genetic purity of the F1 seeds. To investigate the genetics of fertility restoration of the Peterson cytoplasmic sterility in pepper, a doubled haploid population of 115 pepper lines obtained from anther culture of the F1 hybrid between Yolo Wonder (sterility maintainer line) and Perennial (fertility restorer line) and the parental lines were test-crossed by 77013A (a strict cytoplasmic-genic male sterile line). The fertility of the test-crossed lines was assessed in greenhouse and open field with the following three criteria: pollen index (PI, visual estimation of pollen amount per flower), pollen number (PN, pollen counting under microscope), and seed number (SN, the number of seeds per fruit in open pollination). Correlations between the each couple of criteria within, as well as between the cultivation methods ranged from 0.55 to 0.84. Analysis of variance showed that the genotype (DH line) and environment were the significant sources of variation of the fertility. Narrow sense of heritance of fertility restoration ranged from 0.38 to 0.92, depending on the criteria and environment. The distribution of the progeny was continuous between the parental genotypes indicating the quantitative inheritance of fertility restoration. Inferred from segregation according to Snape et al.(1984), the number of segregating genes was estimated to be that three to four genetic factors were involved in pollen traits (PI and PN) and five to eight genetic factors in seed production (SN). The heredity analysis of the CMS will be helpful for understanding of the genetic mechanism of the fertility restoration and the exploitation of the CMS in hybrid seed production. 展开更多
关键词 Capsicum annuum L. doubled haploids population cytoplasmic male sterility (CMS) fertility restoration genetic analysis
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Comparisons on Genetic Diversity among the Isonuclear-Alloplasmic Male Sterile Lines and Their Maintainer Lines in Rice 被引量:1
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作者 LI Jin-quan CAI Shan-xin FENG Jiu-huan LI Wei CHENG Gui-ping 《Rice science》 SCIE 2007年第2期94-100,共7页
Four sets of rice isonuclear-alloplasmic lines including 16 male sterile lines and their maintainer lines were analyzed by using 91 pairs of SSR primers to study the genetic diversity of nuclear genome and their relat... Four sets of rice isonuclear-alloplasmic lines including 16 male sterile lines and their maintainer lines were analyzed by using 91 pairs of SSR primers to study the genetic diversity of nuclear genome and their relative relationships. A total of 169 alleles were detected in the 16 lines, with a frequency of polymorphic loci of 53.85% and an average number of alleles per locus of 1.8, and the average gene diversity was 0.228. Four sets of the isonuclear-alloplasmic male sterile lines shared 146 identical alleles, corresponding to 86.39% of the total alleles; meanwhile, there are 23 different alleles among the tested materials, being 13.61% of the total alleles. On average, 78.70% identical alleles and 21.30% different alleles of the total alleles were detected between the isonuclear-alloplasmic male sterile lines and their maintainer lines. There were 53.85% identical alleles and 46.15% different alleles of the total alleles among the homozygous allonucleus male sterile lines. The fingerprints were established for some male sterile lines and maintainer lines. All the materials tested were divided into three groups at the 0.2 genetic distance based on the cluster analysis. Eight lines of Huanong A and Huayu A (including Huanong B and Huayu B) were in the first group, four lines of Kezhen A (including Kezhen B) in the second group, and four lines of Zhenshan 97A (including Zhenshan 97B) in the third group. For the isonuclear-alloplasmic male sterile lines, the similarity coefficient between Y (Yegong) type and WA (wild abortive) type or between CW (Raoping wild rice) and WA type reached 87-98%. 展开更多
关键词 hybrid rice isonuclear-alloplasmic male sterile line maintainer line genetic diversity
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Genetic Mechanism of Dominant Earliness in Kefeng A,a New Rice Cytoplasmic Male Sterile Line
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作者 XIAO Yu-long Yu Chuan-yuan +3 位作者 LEI Jian-guo LI Ma-zhong JIANG Lin WAN Jian-min 《Rice science》 SCIE 2009年第4期267-273,共7页
Kefeng A is an early maturing indica cytoplasmic male sterile (CMS) line of rice. Combinations derived from Kefeng A and late maturing indica restorer lines showed dominant earliness to various extents. To understan... Kefeng A is an early maturing indica cytoplasmic male sterile (CMS) line of rice. Combinations derived from Kefeng A and late maturing indica restorer lines showed dominant earliness to various extents. To understand the genetic basis of dominant earliness, the genotype of photoperiod-sensitive genes in Kefeng A was analyzed using a complete set of heading time near isogenic lines (NILs) EGO to EG7, ER, LR, T65, T65E^b, T65E^bm, T65m, NIL(Hd1) and NIL(Hd4). Results indicated that Kefeng A contained two dominant photoperiod-sensitive alleles E1 and Se-1^U on E1 and Se-1 loci, respectively, and the genotype of photoperiod-sensitivity genes for heading time in Kefeng A was E1E1e2e2E3E3Se-1^USe-1UEf-1Ef-1. Based on the detected heading time genotype, in combination with the heading time of Kefeng A and the early maturing phenomenon in its derived F1 hybrids, it is speculated that Kefeng A might carry a dominant inhibitor gene Su-E7 for the dominant photoperiod- sensitive gene E1, and a recessive inhibitor gene i-Se-1 for another dominant photoperiod-sensitive gene Se-1. The reason why F1 hybrids from Kefeng A exhibited early maturing was hereby analyzed and the breeding value of dominant earliness related genes in Kefeng A was discussed. 展开更多
关键词 dominant earliness heading time GENOTYPE genetic mechanism cytoplasmic male sterile line RICE
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Study on the Agroclimatic Division of Thermosensitive Genetic Male Sterility Rice Breeding 被引量:1
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作者 陈小敏 陈汇林 +1 位作者 邹海平 王斌 《Agricultural Science & Technology》 CAS 2015年第2期350-354,390,共6页
Using the meteorological data and geographical information during January-March(1961-2010) accumulated by 18 stations of Hainan,a suitability zoning map for thermo-sensitive genetic male sterility(TGMS) rice was m... Using the meteorological data and geographical information during January-March(1961-2010) accumulated by 18 stations of Hainan,a suitability zoning map for thermo-sensitive genetic male sterility(TGMS) rice was made by GIS technology based on temperature indicators required by TGMS rice during fertility sensitive period and heading-flowering period,aiming to provide reasonable layout and scientific basis for sustainable development of TGMS rice in Hainan Island under the background of global warming.The results indicated that the suitable planting zones covered the south regions of Wuzhishan,Jianfengling and Diaoluoshan;subordinate suitable zones expanded northward to central parts of Wuzhishan,Dongfang,Changjiang,Qiongzhong and southern parts of Wanning;the other regions were unsuitable for breeding. 展开更多
关键词 Thermo-sensitive genetic male sterility(TGMS) rice Fertility sensitive period Heading-flowering period Agroclimatic division HAINAN
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Genetic Study on Barley Male Sterility 被引量:1
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作者 张凤英 刘志萍 包海柱 《Agricultural Science & Technology》 CAS 2009年第4期108-111,共4页
[Objective] The aim was to research the genetic mechanism of barley male sterility, and provide the theoretical reference for breeding strong heterosis hybrid of barley. [ Method] Fertility segregation phenomenon, mor... [Objective] The aim was to research the genetic mechanism of barley male sterility, and provide the theoretical reference for breeding strong heterosis hybrid of barley. [ Method] Fertility segregation phenomenon, morphological characteristics and main agronomic characters of male sterility character of 4 kinds of barley male sterility materials 2001 - 17, 2001 - 37, 2001 - 84 and 2001 - 116 and their dedved lines were observed and researched.[ Result] Barley male sterility existed genetic phenomenon of single dominant nuclear gene caused by environmental stimulation, its sterility controlled by MS, the sterile genotype was MSms. There was stable nucleo-cytoplasmic inheritance on barley male sterility which was controlled by cytoplasmic mate sterile gene S and nuclear gene rr, and its genotype was S(rr), this male sterility belonged to CMS type sterility. Temperature had no effect on fertility. [ Conclusion] There was stable nucleo-cytoplasmic inheritance on barley male sterility, this result played a positively promoting role in barley practical production. 展开更多
关键词 BARLEY male sterility genetic mechanism
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Creation and Genetic Analysis of a Male Sterility Mutant in Panicum miliaceum
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作者 李海权 相金英 +4 位作者 韩玉翠 降彦苗 耿玲玲 程汝宏 刘国庆 《Agricultural Science & Technology》 CAS 2017年第7期1187-1191,1231,共6页
Heterosis plays an important role in the development of new crop varieties with high-yielding, good-quality and biotic/abiotic stresses while male sterile line de- velopment is the key step to determine the success of... Heterosis plays an important role in the development of new crop varieties with high-yielding, good-quality and biotic/abiotic stresses while male sterile line de- velopment is the key step to determine the success of heterosis utilization. A male sterile mutant, M207A was created in proso millet (Panicum mi/iaceurn, 2n=4x=36) for the first time using 60Co-y ray mutagenesis. Fertility identification and genetic analysis were carried out to characterize the mutant for its possible use for hetero- sis utilization in proso millet. First the sterility was investigated using both field sur- vey and indoor pollen microscopy identification. Then Pollinated by normal fertile proso millet cultivars, F1 and F2 populations from the mutant were obtained. Mean- while primary genetic analysis was also conducted using above populations in dif- ferent experimental sites, seasons and years. The results showed that the male sterile plant exhibited closed glumes, browning and dry anthers with few normal pollens. The sterility was stable and sterility rate was above 95% on average. The segregation ratio of fertile to sterile plants was 35:1 in the fertile selfing F2 popula- tion indicating that the mutant was a genic male sterility belonging to a pollen-less type controlled by a single recessive gene. The creation of the mutant, M207A can play a key role for heterosis utilization in proso millet. 展开更多
关键词 Panicum mi/iaceum Radiation mutagenesis male sterile mutant genetic analysis Hoterosis utilization
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Genetic analysis of fertility restoring genes for AL-type male sterility in wheat 被引量:4
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作者 Liu Xiaofang Tian Xiaoming +6 位作者 Nie Yingbin Mu Peiyuan Han Xinnian Sang Wei Cui Fengjuan Xu Hongjun Xiang Jishan 《Engineering Sciences》 EI 2013年第5期30-36,共7页
In order to screen molecular markers linked to fertility restoring genes and further improve the breeding efficiency of restorer lines, in this study, wheat varieties 18A, 18B and 99AR144-1 were used as experimental m... In order to screen molecular markers linked to fertility restoring genes and further improve the breeding efficiency of restorer lines, in this study, wheat varieties 18A, 18B and 99AR144-1 were used as experimental materials to establish F2 fertility-segregating population. Plant quantitative trait "major gene + polygene mixed mo- del" separation analysis method and simple sequence repeat (SSR) molecular markers were adopted for genetic analysis of four generations, including the parents (P~ and P2), and hybrid (G and G) populations. The results show that AL-type fertility restoring gene is controlled by two pairs of additive-dominant-epistatic genes and addi- tive-dominant polygene; two primers linked to fertility restoring genes were selected by SSR molecular markers, including Xgwm95 on chromosome 2A and Barc61 on chromosome 1B, with the linkage distance of 15.0 cM and 18.0 cM, respectively. Based on verification, these two markers are reliable for distinguishing AL-type wheat ste- rile lines and restorer lines. 展开更多
关键词 WHEAT cytoplasmic male sterility (CMS) restoring gene genetic analysis SSR molecular marker
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A Review on Creating Male Sterility in Vegetable Crops by Genetic Engineering 被引量:1
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作者 C. Q. Yang Y. G. Oin +3 位作者 J. J. Lei L. Zhai B. H. Cao G. J, Chen 《Journal of Agricultural Science and Technology(B)》 2011年第8期1097-1102,共6页
With the deep researches on male sterility genetic engineering of plants, several strategies creating male sterile materials have been developed, such as causing pollen abortion by cytotoxic genes, antisense RNA or RN... With the deep researches on male sterility genetic engineering of plants, several strategies creating male sterile materials have been developed, such as causing pollen abortion by cytotoxic genes, antisense RNA or RNAi silencing the expression of genes related to pollen development, early degradation of tapetum callose leading to male sterility. Male sterile transgenic plants can be obtained through genetic transformation with related genes destroying or interfering with pollen or anther development. Male sterile cauliflower, tomato, cabbage, etc. have been developed in this way, and some begin to be used to produce hybrid seed. Appling some techniques can also maintain and restore the male sterility. These related researches will effectively promote the heterosis utilization and the development of crop breeding. This paper mainly presents their principles and applications in vegetable crops. 展开更多
关键词 VEGETABLE genetic engineering male sterility BREEDING review.
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Mapping of a gene for photoperiod-sensitive genic male sterility in Nongken 58s on chromosome 5 of rice
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作者 LIN Xinghua,YU Gongxin,ZHANG Duanpin,XIE Yuefeng,QIN Falan,State key laboratory in genetic improve ment of crops ,Huahong,Agri Univ,Wuhan 430070,China 《Chinese Rice Research Newsletter》 1996年第3期3-4,共2页
A photoperiod-sensitive genic male sterile (PGMS) rice was found in 1973 as a spontaneous mutant of Nongken 58, a japonica variety. Pollen fertility of Nongken 58s (N58s) is completely sterile when grown under long-da... A photoperiod-sensitive genic male sterile (PGMS) rice was found in 1973 as a spontaneous mutant of Nongken 58, a japonica variety. Pollen fertility of Nongken 58s (N58s) is completely sterile when grown under long-day conditions, whereas fertile under short-day conditions. This PGMS was found to be controlled by one or two recessive gene(s), of which one gene(pms)was linked to a marker gene(d-1) on chromosome 5. In order to identify a more precise location of the pms, we analyzed the populations of BCFand BCFof N58s//N58s/KL211(v-10, virescent) and N58s//N58s/KL520 (gh-1, gold hull). The marker genes v-10 and gh-1 are located on the flanking region of d-1. The F, plants of two crosses were fertile. The number of fertile and sterile individuals in BCFfit 展开更多
关键词 Mapping of a gene for photoperiod-sensitive genic male sterility in Nongken 58s on chromosome 5 of rice GENE
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Genetic analysis of fertility restoration of Maxie cytoplasmic male sterility in rice(Oryza sativa L.)
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作者 ZHANG Xiaoguo ZHU Yingguo and MEI Qiming,College of Life Sci,Wuhan Univ,Wuhan 430072,China 《Chinese Rice Research Newsletter》 1996年第4期1-2,共2页
The genetic behavior of fertility restorationgenes of the cytoplasmic male sterile line Max-ie A was studied to facilitate the use ofMaweizhan male sterile cytoplasm.The F,F,and Fof Maxie A/Minhui 63 were grownin padd... The genetic behavior of fertility restorationgenes of the cytoplasmic male sterile line Max-ie A was studied to facilitate the use ofMaweizhan male sterile cytoplasm.The F,F,and Fof Maxie A/Minhui 63 were grownin paddy field,1993-1995.Meanwhile,someof the Fplants were testcrossed with themaintainer of Maxie A.The fertilities were 展开更多
关键词 Oryza sativa L genetic analysis of fertility restoration of Maxie cytoplasmic male sterility in rice
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Preliminary Study on Inheritance of Stigma Exertion in Wheat Thermo-photo Sensitive Genic Male Sterile Line
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作者 李宏生 丁明亮 +6 位作者 顾坚 田玉仙 赵红 吴绍柱 杨素梅 杨和仙 李绍祥 《Agricultural Science & Technology》 CAS 2015年第9期1930-1932,1938,共4页
Stigma exertion is one of the key factors for improving the outcrossing ability of wheat thermo-photo sensitive genic male sterile(TPSGMS) line. A DH population derived from K239S/K92 S was constructed to investigat... Stigma exertion is one of the key factors for improving the outcrossing ability of wheat thermo-photo sensitive genic male sterile(TPSGMS) line. A DH population derived from K239S/K92 S was constructed to investigate the inheritance of stigma exertion. K239 S and K92 S are TPSGMS lines with higher and lower stigma exertion rates(SER), respectively. The SERs of parents, reciprocal crosses and the DH population were evaluated for two consecutive years. The results showed that no significant difference was observed in SER between F1 s of K239S/K92 S and K92S/K239 S,implying that stigma exertion was a trait controlled by nuclear gene(s). In the DH population, the segregation of low and high SERs fitted to a ratio of 3 ∶1 by Chisquare test, suggesting that the stigma exertion of K239 S was controlled by one pair of recessive genes. In addition, the effects of temperature and humidity on the expression of stigma exertion were also discussed. 展开更多
关键词 WHEAT Thermo-photo sensitive genic male sterile line Stigma exertion rate genetic analysis
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Development and Application of SCAR Markers for Discriminating Cytoplasmic Male Sterile Lines from Their Cognate Maintainer Lines in Indica Rice 被引量:2
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作者 LU Chao LIU Jian +4 位作者 JIANG Jian-hua Caleb Manamik BRERIA TAN He-lin Masahiko ICHII HONG De-lin 《Rice science》 SCIE 2013年第3期191-199,共9页
The DNAfragments about 1 600 bp were amplified using random amplified polymorphism DNA (RAPD) primer OPAl2 with the templates of mitochondrial DNA of Zhenshan 97A and Zhenshan 97B, and were sequenced. The nucleotide... The DNAfragments about 1 600 bp were amplified using random amplified polymorphism DNA (RAPD) primer OPAl2 with the templates of mitochondrial DNA of Zhenshan 97A and Zhenshan 97B, and were sequenced. The nucleotide sequences and lengths of the fragments from Zhenshan 97A and Zhenshan 97B showed no difference. The precise length of the fragment was 1 588 bp. Sequence characterized amplification region (SCAR) primers were then developed to discriminate the cytoplasmic male sterile (CMS) lines and their maintainer lines. A specific 1 588 bp fragment could be amplified with SCAR primers, CHI19F2/CHI19R2 and CHI20F3/CHI23R3, in the mitochondrial DNA of Zhenshan 97A, but not Zhenshan 97B. Furthermore, the specific fragment could be also amplified from the total DNA from green leaf tissues of Zhenshan 97A with SCAR primers, but not Zhenshan 97B. With the corresponding primers, the specific fragment could also be amplified from the total DNA of green leaves of other two CMS lines with wild abortive type cytoplasm (CMS-WA), namely Zhenpin A and Tianfeng A, but not in their maintainer lines. Moreover, using total DNA as template, each of the four pairs of SCAR primers could also be used to amplify the 1 588 bp fragment in CMS-ID (Indonesia paddy type) line 11-32A but not in 11-32B, and the specific fragment was amplified from the DNA of both F1 and F2 seedlings of Shanyou 63. The results of detecting the genetic purity of a man-made mixture of the seeds of Zhenshan 97A using CHI20F3/CHI23R3 were completely consistent with the phenotypes. Taken together, these results indicated that the specific 1 588 bp-fragment amplified by CHI20F3/CHI23R3 was the unique amplification products of CMS mitochondrial DNA, and could be used to distinguish CMS-WA and CMS-ID lines from their corresponding maintainer lines at the seedling stage. 展开更多
关键词 Oryza safiva cytoplasmic male sterile line maintainer line genetic purity sequence characterized amplification region marker
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Morphological and cytological assessments reveal pollen degradation causes pollen abortion in cotton cytoplasmic male sterility lines
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作者 PEI Qingyu LIU Jinshan +10 位作者 GUO Chunping MA Xiaomei LIU Xiaoyan YOU Chunyuan LIN Hairong LI Zhibo ZHAO Ruihai ZHU Bo WU Yuanlong PAN Zhenyuan NIE Xinhui 《Journal of Cotton Research》 CAS 2024年第3期266-276,共11页
Background Understanding the mechanism of male sterility is crucial for producing hybrid seeds and developing sterile germplasm resources.However,only a few cytoplasmic male sterility(CMS)lines of cotton have been pro... Background Understanding the mechanism of male sterility is crucial for producing hybrid seeds and developing sterile germplasm resources.However,only a few cytoplasmic male sterility(CMS)lines of cotton have been produced due to several challenges,like inadequate variation of agronomic traits,incomplete sterility,weak resilience of restorer lines,and difficulty in combining strong dominance.Therefore,the morphological and cytological identification of CMS in cotton will facilitate hybrid breeding.Results Two F_(2) segregating populations of cotton were constructed from cytoplasmic male sterile lines(HaA and 01A,maternal)and restorer lines(HaR and 26R,paternal).Genetic analysis of these populations revealed a segregation ratio of 3:1 for fertile to sterile plants.Phenotypic analysis indicated no significant differences in traits of flower bud development between sterile and fertile plants.However,sterile plants exhibited smaller floral organs,shortened filament lengths,and anther atrophy on the flowering day in comparison with the fertile plants.When performed scanning electron microscopy(SEM),the two F_(2) populations revealed morphological variations in the anther epidermis.Cellular analysis showed no significant differences in pollen development before pollen maturation.Interestingly,between the pollen maturation and flowering stages,the tapetum layer of sterile plants degenerated prematurely,resulting in abnormal pollen grains and gradual pollen degradation.Conclusion The results of this study suggest that fertility-restoring genes are controlled by a single dominant gene.Sterile plants exhibit distinctive floral morphology,which is characterized by stamen atrophy and abnormal anthers.Pollen abortion occurs between pollen maturity and flowering,indicating that premature tapetum degradation may be the primary cause of pollen abortion.Overall,our study provides a theoretical basis for utilizing CMS in hybrid breeding and in-depth investigation of the dominant configuration of cotton hybrid combinations,mechanisms of sterility,and the role of sterile and restorer genes. 展开更多
关键词 COTTON Cytoplasmic male sterility genetic analysis Morphological characteristics Pollen development
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Studies on the Photoperiod Sensitive Characters of Male Fertility Alteration of Peiai64S' Main Male Genic Sterile Gene
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作者 ZENG Han-lai,ZHANG Duan-pin,ZHANG Zhi-yu,YI Wen-kai,ZHU Xin and MENG Hui-jun(National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University , Wuhan 430070 , P. R . China ) 《Agricultural Sciences in China》 CAS CSCD 2002年第5期481-485,共5页
Peiai64S, an indica male sterile rice with a male fertility alteration under different environments, is selected from the offspring of indica rice crossed with Nongken58S. Nongken58S, a japonica pho-toperiod sensitive... Peiai64S, an indica male sterile rice with a male fertility alteration under different environments, is selected from the offspring of indica rice crossed with Nongken58S. Nongken58S, a japonica pho-toperiod sensitive genie male sterile rice (PGMS), deriving from a natural mutant plant individual of normal japonica rice variety, Nongken58, is used as a male sterile gene donor of Peiai64S. But Peiai64S is not a typical PGMS rice, the male fertility is sensitive to temperature just as thermo-sensitive genie male sterile rice (TGMS). We have selected typical PGMS plants in F2 population of Peiai64S× Nongken58, whose ratio of fertile plants to sterile plants is nearly 3:1. The sterility inheritance conformed to one pair of gene segregation model. The result indicates the main male sterile gene in Peiai64S is not other than the PGMS gene, and comes from Nongken58S. The genetic background affects effective expression of the PGMS gene. This suggests that we ought to focus on optimizing the genetic background of the PGMS gene in PGMS rice breeding, and select an ideal genetic background as a transgenic background in molecular breeding. 展开更多
关键词 Peiai64S photoperiod-sensitive genie male sterility (PGMS) genetic background Gene expression
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Rapid Constructing a Genetic Linkage Map by AFLP Technique and Mapping a New Gene tms5 被引量:9
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作者 贾建航 李传友 +1 位作者 邓启云 王斌 《Acta Botanica Sinica》 CSCD 2003年第5期614-620,共7页
In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F-2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkag... In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F-2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkage map of rice. Total of 142 newly screened AFLP markers and 30 anchor markers (25 SSR markers and 5 RFLP markers) were mapped on the 12 chromosomes covering 1537.4 cM of rice genome. The average interval between these markers was 9.0 cM. The total work which usually was finished in more than one year was finished within only 3 months by one person. This is the first plant AFLP map developed in China. A new thermosensitive genic male sterile gene in rice, tms5, was Egged and mapped onto chromosome 2 during the development of the linkage map. 展开更多
关键词 AFLP rapid constructing genetic linkage map rice thermosensitive genic male sterile gene tms5
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Influence of Soil Moisture and Air Temperature on the Stability of Cytoplasmic Male Sterility (CMS) in Maize (Zea mays L.) 被引量:3
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作者 Heidrun Bueckmann Katja Thiele Joachim Schiemann 《Agricultural Sciences》 2016年第2期70-81,共12页
Cytoplasmic male sterility (CMS) is a maternally inherited trait that suppresses the production of viable pollen. CMS is a useful biological tool for confinement strategies to facilitate coexistence of genetically mod... Cytoplasmic male sterility (CMS) is a maternally inherited trait that suppresses the production of viable pollen. CMS is a useful biological tool for confinement strategies to facilitate coexistence of genetically modified (GM) and non-GM crops in case where it is required. The trait is reversible and can be restored to fertility in the presence of nuclear restorer genes (Rf genes) and by environmental impacts. The aim of this study was to investigate the influence of the level of irrigation on the stability of CMS maize hybrids under defined greenhouse conditions. Additionally the combination of irrigation and air temperature was studied. Three CMS maize hybrids were grown with different levels of irrigation and in different temperature regimes. Tassel characteristics, pollen production and fertility were assessed. The CMS stability was high in hot air temperatures and decreased in lower temperatures. The level of irrigation had no major effect on the level of sterility. The extent of these phenomena was depending on the genotype of CMS maize and should be known before using CMS for coexistence purposes. 展开更多
关键词 Soil Moisture Air Temperature Biological Confinement Cytoplasmic male sterility (CMS) genetically Modified (GM) Maize (Zea mays L.)
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Selection and Characterization of a Novel Photoperiod-Sensitive Male Sterile Line in Upland Cotton 被引量:3
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作者 Jianhui Ma Hengling Wei +6 位作者 Ji Liu Meizhen Song Chaoyou Pang Long Wang Wenxiang Zhang Shuli Fan Shuxun Yu 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2013年第7期608-618,共11页
Upland cotton (Gossypium hirsutum L.) shows strong heterosis. However, heterosis is not widely utilized owing to the high cost of hybrid seed production. Creation of a photoperiod-sensitive genetic male sterile line... Upland cotton (Gossypium hirsutum L.) shows strong heterosis. However, heterosis is not widely utilized owing to the high cost of hybrid seed production. Creation of a photoperiod-sensitive genetic male sterile line could substantially reduce the cost of hybrid seed production in upland cotton. Such a mutant with virescent marker was found by space mutation in near-earth orbit and its traits had been stable after 4 years of selection in Anyang and Sanya, China. This mutant was fertile with an 11-12.5 h photoperiod when the temperature was higher than 21.5 ℃ and was sterile with a 13-14.5 h photoperiod. Genetic analysis indicated that both traits were controlled by a single recessive gene or two closely linked genes. Also, the cytological observations and transcriptome profiling analysis showed that the degradation of pollen grain cytoplasm should be the primary reason why the mutant line were male sterile under long-day conditions. 展开更多
关键词 photoperiod-sensitive genetic male sterile sterility mechanism upland cotton virescent marker.
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Cytological Investigation of Pollen Development in Sorghum Line with Male Sterility Induced by Sodium Ascorbate in Tissue Culture
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作者 Marina I. Tsvetova Lev A. Elkonin 《American Journal of Plant Sciences》 2013年第7期11-18,共8页
Investigation of male sterility mutations is an effective approach for identification of genes involved in anther and pollen development. The comparison of “cytological phenotypes” of newly induced mutants with phen... Investigation of male sterility mutations is an effective approach for identification of genes involved in anther and pollen development. The comparison of “cytological phenotypes” of newly induced mutants with phenotypes determined by already known genes favors elucidation of genetic control of diverse microsporo- and gametogenesis stages. In this paper, we describe pollen development in the grain sorghum line Zh10-asc1 with mutation of male sterility. This line was obtained from callus culture treated by sodium ascorbate. A wide spectrum of abnormalities in microsporogenesis have been found, such as cytomixis, chromosomal laggards, chromosome disjunction, adhesion of chromosomes, disturbed cytokinesis, and others. In tapetum, the cells with one nucleus, with unequal nuclei, and with micronuclei have been observed. During pollen grain (PG) maturation abnormalities in starch accumulation and delay of development often took place. In mature anthers, a variety of pollen grain types have been revealed: fertile, of irregular shape, incompletely filled with starch, PGs delayed at the uni-nucleate or bi-nucleate gametophyte stages, with partially or fully degenerated contents, and with abnormal coloration. Variation in spectrum and the frequency of disturbances between the flowers of one and the same plant have been revealed. The reasons for significant genetic and epigenetic instability are discussed. 展开更多
关键词 male sterilITY MICROSPOROGENESIS Sodium ASCORBATE In Vitro Mutagenesis genetic Instability SORGHUM BICOLOR (L.) Moench
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pms3 is the locus causing the original photoperiod-sensitive male sterility mutation of‘Nongken 58S' 被引量:15
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作者 梅明华 陈亮 +3 位作者 章志宏 李子银 徐才国 张启发 《Science China(Life Sciences)》 SCIE CAS 1999年第3期316-322,共7页
Photoperiod-sensitive genie male sterile (PSGMS) rice is a very useful germplasm for hybrid rice development. It was first found as a spontaneous mutant in a japonic a cultivar 'Nongken 58' . pms3 on chromosom... Photoperiod-sensitive genie male sterile (PSGMS) rice is a very useful germplasm for hybrid rice development. It was first found as a spontaneous mutant in a japonic a cultivar 'Nongken 58' . pms3 on chromosome 12 was determined to be the locus where the original PSGMS mutation occurred, changing the normal cultivar Nongken 58 to PSGMS Nongken 58S. Large amounts of RAPD and AFLP analyses were also conducted for the fine mapping of the pms3 genomic region, which resulted in 4 molecular markers linked to pms3. Although these markers somewhat increased the marker density of this region, the pms3 locus is still located in a marker-sparse region. 展开更多
关键词 photoperiod-sensitive genie male sterile (PSGMS) rice gene mapping restriction FRAGMENT LENGTH POLYMORPHISM (RFLP) randomly amplified polymorphic DNA (RAPD) amplified FRAGMENT LENGTH POLYMORPHISM (AFLP).
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