肿瘤相关巨噬细胞内Notch⁃1通过抑制组织蛋白酶S过表达影响非小细胞肺癌腺癌侵袭转移的机制研究

目的探讨Notch-1在合并及未合并肝转移非小细胞肺癌腺癌患者肿瘤相关巨噬细胞内的表达情况,并通过体外细胞培养分析其对癌细胞侵袭转移的影响及相关机制。方法收集2021年1月至2022年12月收治的64例非小细胞肺癌腺癌患者标本,并分为未合并肝转移组32例和合并肝转移组32例,利用流式分选法分离CD68+肿瘤相关巨噬细胞,应用Western blot法检测2组CD68+肿瘤相关巨噬细胞Notch-1及相关蛋白表达水平。采用体外培养人单核/巨噬细胞THP-1细胞分别感染Notch-1 NC(阴性对照)和shRNA慢病毒载体后与人非小细胞肺癌A549共培养,应用Western blot法检测THP-1细胞相关蛋白表达情况,Transwell实验及划痕实验检测共培养体系中A549细胞迁移及侵袭能力。结果与未合并肝转移组比较,合并肝转移组CD68+肿瘤相关巨噬细胞内Notch-1表达水平显著降低,并伴随精氨酸酶-1及组织蛋白酶S表达水平显著增高(P<0.05)。与Notch-1 NC组比较,Notch-1 shRNA组THP-1细胞Notch-1表达水平显著降低,并伴随组织蛋白酶S表达水平升高(P<0.05)。与感染Notch-1 shRNA的THP-1细胞共培养后抑制表达Notch-1的A549细胞侵袭和迁移细胞数目明显增多,且细胞划痕距离明显减小(P<0.05)。结论肿瘤相关巨噬细胞内Notch-1蛋白表达具有抑制非小细胞肺癌腺癌细胞侵袭及转移的作用。

Model and method of permeability evaluation based on mud invasion effects

The evaluation of permeability in reservoir assessment is a complex problem. Thus, it is difficult to perform direct evaluation permeability with conventional well-logging methods. Considering that reservoir permeability significantly affects mud invasion during drilling, we derive a mathematical model to assess the reservoir permeability based on mud invasion. A numerical model is first used to simulate the process of mud invasion and mud cake growth. Then, based on Darcy＇s law, an approximation is derived to associate the depth of mud invasion with reservoir permeability. A mathematical model is constructed to evaluate the reservoir permeability as a function of the mud invasion depth in time-lapse logging. Sensitivity analyses of the reservoir porosity, permeability, and water saturation are performed, and the results suggest that the proposed model and method are well suited for oil layers or oil-water layers of low porosity and low permeability. Numerical simulations using field logging and coring data suggest that the evaluated and assumed permeability data agree, validating the proposed model and method.

“Speech on Hitler’s Invasion of the U.S.S.R.”(汉译文)的美学赏析

语言作为人类精神的产物和文明的载体应具有审美价值,而演讲作为一种特殊的语言表现形式,它的审美价值在于能够使受众在愉悦中自觉地去接受或容纳演讲的主旨。Speech on Hitler’s Invasion of the U.S.S.R.是丘吉尔政治生涯中重要的演讲作品之一,既具有时效性和实效性,又具有历久弥新的语言审美价值。

经皮微创、Wiltse间隙微创入路与传统切开后入路治疗老年胸腰椎骨折临床疗效

目的探讨经皮微创、Wiltse间隙微创及传统切开后入路联合伤椎椎弓根内固定术治疗老年胸腰椎骨折的临床效果。方法回顾性分析2018年3月至2021年12月诊断为胸腰椎骨折的老年患者98例的临床资料。根据手术入路的不同,将患者分为经皮组、Wiltse组和切开组,经皮组31例,其中男14例,女17例。平均年龄(69.3±6.6)岁;Wiltse组33例,男12例,女21例;平均年龄(68.7±7.1)岁;切开组34例,其中男16例,女18例,平均年龄(67.7±6.9)岁。比较3组患者的手术时间、术中出血量、术后引流量、透视次数、住院时间、Cobb′s角、伤椎椎体前缘高度、视觉模拟评分(VAS)、Oswestry功能障碍指数(ODI)及相关并发症。结果3组术后伤椎前缘高度和Cobb′s角较术前均有明显改善(P<0.05),术后3组组间比较差异无统计学意义(P>0.05)。3组患者围手术期各项观察指标对比,经皮组的手术时间和透视次数比其他两组明显较多,差异有统计学意义(P<0.05);切开组术中出血量、住院时间与其他两组比较差异有统计学意义(P<0.05);3组间的术后引流量相互比较差异均有统计学意义(P<0.05)。3组患者术后VAS评分及ODI值较术前均降低(P<0.05),且经皮组及Wiltse组患者术后的VAS评分和ODI值均优于切开组患者(P<0.05)。切开组术后出现手术及基础疾病相关并发症较多,与其他两组比较差异有统计学意义(P<0.05)。结论治疗老年胸腰椎骨折,3种方法均可取得较好的临床效果,但经皮及Wiltse间隙微创入路相较于传统切开在软组织保护和术后功能康复方面更具优势,临床应用还需根据患者具体情况,采用合适的治疗方式才能够取得更好的疗效。

High expression of ErbB2 contributes to cholangiocarcinoma cell invasion and proliferation through AKT/p70S6K

AIM:To compare the impact of ErbB2 on cell invasion and proliferation in cholangiocarcinoma(CCA) cell lines.METHODS:Level of endogenous ErbB2 expression in three CCA cell lines,namely HuCCA-1,KKU-100 and KKU-M213,was determined by real-time reversetranscriptase polymerase chain reaction.Two ErbB2 inhibitory methods,a small molecule ErbB2 kinase inhibitor(AG825) and siRNA,were used to disrupt ErbB2 function in the cell lines.CCA cell invasion,motility and proliferation under ErbB2-disrupted conditions were detected using Transwell and 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide assays.In addition,ErbB2 downstream effectors were investigated by Western blotting analysis.RESULTS:Suppression of ErbB2 activity,using a specific kinase inhibitor(AG825),reduced invasion,motility and proliferation of all three CCA cell lines.The ability of this drug to inhibit neoplastic properties(invasion,motility and proliferation) increased concomitantly with the level of ErbB2 expression.Similarly,knockdown of ErbB2 level by siRNA inhibited cell invasion and proliferation of KKU-M213,a high-ErbB2-expressing cell,better than those of the lower-ErbB2-expressing cells,HuCCA-1 and KKU-100.Thus,both inhibitory methods indicated that there is more ErbB2-dependency for malignancy of the high-ErbB2-expressing cell,KKU-M213,than for that of low-ErbB2-expressing ones.In addition,interrupting ErbB2 activity decreased phosphorylation of AKT and p70S6K,but not extracellular signal-regulated kinase 1/2,in the high-ErbB2-expressing CCA cell line.CONCLUSION:Our data indicated that high ErbB2 expression enhances CCA invasion,motility and proliferation via the AKT/p70S6K pathway,which suggests the possibility of targeting these molecules for CCA therapy.

lncRNACNN3-206 activates intestinal epithelial cell apoptosis and invasion by sponging miR-212, an implication for Crohn’s disease

BACKGROUND Statistics indicate that the incidence of Crohn’s disease(CD)is rising in many countries.The poor understanding on the pathological mechanism has limited the development of effective therapy against this disease.Previous studies showed that long noncoding RNAs(lncRNAs)could be involved in autoimmune diseases including CD,but the detailed molecular mechanisms remain unclear.AIM To identify the differentially expressed lncRNAs in the intestinal mucosa associated with CD,and to characterize their pathogenic role(s)and related mechanisms.METHODS The differential expression of lncRNAs was screened by high-throughput RNA sequencing,and the top candidate genes were validated in an expanded cohort by real-time PCR.The regulatory network was predicted by bioinformatic software and competitive endogenous RNA analysis,and was characterized in Caco-2 and HT-29 cell culture using methods of cell transfection,real-time PCR,Western blotting analysis,flow cytometry,and cell migration and invasion assays.Finally,these findings were confirmed in vivo using a CD animal model.RESULTS The 3'end of lncRNACNN3-206 and the 3’UTR of Caspase10 contain highaffinity miR212 binding sites.lncRNACNN3-206 expression was found to be significantly increased in intestinal lesions of CD patients.Activation of the lncRNACNN3-206-miR-212-Caspase10 regulatory network led to increased apoptosis,migration and invasion in intestinal epithelial cells.Knockdown of lncRNACNN3-206 expression alleviated intestinal mucosal inflammation and tissue damage in the CD mouse model.CONCLUSION lncRNACNN3-206 may play a key role in CD pathogenesis.lncRNACNN3-206 could be a therapeutic target for CD treatment.

Thymoquinone Suppresses Cellular Proliferation, Inhibits VEGF Production and Obstructs Tumor Progression and Invasion in the Rat Model of DMH-Induced Colon Carcinogenesis

A myriad of medicinal effects has been attributed to Thymoquinone (TQ), the major biological-active component of Nigella sativa. TQ has been shown to exhibit potent anti-tumor activities. The present work was undertaken to further explore TQ’s chemopreventive efficacy against 1, 2-dimethylhydrazine (DMH)-induced colon carcinogenesis in the rat model through a two-phase study (initiation and post-initiation) and to evaluate its potential impact on tumor progression and invasion in vivo. TQ treatment in the initiation phase significantly reduced tumor incidence, multiplicity and mean tumor volume. However, although mean tumor volume and multiplicity were decreased upon TQ treatment in the post-initiation phase, TQ did not reduce incidence significantly. Cellular proliferation, as assessed by expression of colonic PCNA, was shown to be inhibited in consequence to TQ treatment in both phases, with a more pronounced reduction in the initiation phase. In addition, our results demonstrated an appreciable negative impact of TQ on vascular endothelial growth factor (VEGF) production in tumor-bearing rats. Furthermore, we provided evidence that TQ-treatment, in both phases, tended to considerably suppress tumor progression and invasion. Taken together, the present study demonstrated that TQ, at an orally daily dose of 10 mg/kg, has a chemopreventive effect in the initiation phase, and has the potential to attenuate tumor burden, suppress progression of pre-neoplastic lesions and to inhibit tumor growth in the post-initiation phase of DMH-induced colon carcinogenesis, We surmise that such effects of TQ may be due to suppression of cellular proliferation and inhibition of VEGF production. The results could provide an effective chemopreventive approach in the primary prevention of colon cancer in humans in the next future, and illuminate a promising horizon to combat progression of benign colonic pre-neoplastic lesions into malignant metastatic tumors and to manage colon cancer.

Fluctuating resources,disturbance and plant strategies:diverse mechanisms underlying plant invasions

This paper examines the hypothesis that non-native plant invasions are related to fluctuating resource availability as proposed by Davis et al. （2000）. I measured relative functional responses of both invasive and native plants to changed resource availability due to nutrient enrichment and rainfall, and to increased disturbance. Data are presented from studies in two contrasting ecosystems. First is a series of glasshouse and field experiments on the invader Hieracium lepidulum and associated invasive and native species in subalpine temperate New Zealand. Second is a field study of invasive and native plant responses to altered disturbance regimes and rainfall from tropical savannas of north eastern Australia. Invaders responded differently from native species to changes in resource availability in both subalpine and tropical studies. However, invaders differed among themselves showing that different species exploit different functional niches to invade their respective habitats. These findings contribute to the contention that the fluctuating resource hypothesis does not provide a universal explanation for plant invasions. The diverse functional responses to increased resource availability among invaders in this and previous studies suggest that the cause of invasion depends on unique combinations of habitat and functional attributes of invaders and native assemblages. Such findings imply that universal predictions of what will happen under climate change scenarios across the globe will be difficult to make.

Knockdown of the Meq gene in Marek’s disease tumor cell line MSB1 might induce cell apoptosis and inhibit cell proliferation and invasion

Marek’s disease(MD),a highly cell-associated and contagious disease of chickens caused by Marek’s disease virus(MDV)can result in neural lesions,immunosuppression and neoplasia in chicken.The Meq gene is an important oncogene in the MDV genome,and it is expressed highly in MD tumor tissues and MD T-lymphoblastoid cell lines.An experiment was conducted to elucidate the role of Meq in MD tumor transformation.RNA interference technology was used to block its expression,and then analyzed the biological effects of Meq knockdown on the MD tumor cell line MSB1.A small interfering RNA with an interference efficiency of 70%(P<0.01)was transfected into MSB1 cells to knock down the expression of Meq gene.The cell proliferation,cycle and apoptosis were detected post-Meq knockdown.The results showed that MSB1 cell proliferation was downregulated remarkably at 48 h(P<0.01),60 h(P<0.05)and 72 h(P<0.01)post-Meq knockdown.The cell cycle was unaffected(P>0.05).B-cell lymphoma 2 gene(BCL2)was anti-apoptotic and caspase-6 was the effector in the apoptosis pathway.The activity of caspase-6 was upregulated(P<0.05)significantly and BCL2 gene expression was downregulated(P<0.05)significantly post-Meq knockdown,suggesting cell apoptosis might be induced.MSB1 cell migration did not exhibit any obvious change(P>0.05)post-Meq knockdown,but the expression of two genes(matrix metalloproteinase 2(MMP2)and MMP9)that are correlated closely to cell invasion was downregulated(P<0.05)remarkably post-Meq knockdown.The Meq knockdown might affect the main features of tumorous cells,including proliferation,apoptosis,and invasion,suggesting that the Meq gene might play a crucial role in interfering with lymphomatous cell transformation.

“Parenchyma transection-first”strategy is superior to“tunnel-first”strategy in robotic spleen-preserving distal pancreatectomy with conservation of splenic vessels

Background: Creating a tunnel between the pancreas and splenic vessels followed by pancreatic parenchyma transection(“tunnel-first” strategy) has long been used in spleen-preserving distal pancreatectomy(SPDP) with splenic vessel preservation(Kimura’s procedure). However, the operation space is limited in the tunnel, leading to the risks of bleeding and difficulties in suturing. We adopted the pancreatic “parenchyma transection-first” strategy to optimize Kimura’s procedure. Methods: The clinical data of consecutive patients who underwent robotic SPDP with Kimura’s procedure between January 2017 and September 2022 at our center were retrieved. The cohort was classified into a “parenchyma transection-first” strategy(P-F) group and a “tunnel-first” strategy(T-F) group and analyzed. Results: A total of 91 patients were enrolled in this cohort, with 49 in the T-F group and 42 in the P-F group. Compared with the T-F group, the P-F group had significantly shorter operative time(146.1 ± 39.2 min vs. 174.9 ± 46.6 min, P < 0.01) and lower estimated blood loss [40.0(20.0–55.0) m L vs. 50.0(20.0–100.0) m L, P = 0.03]. Failure of splenic vessel preservation occurred in 10.2% patients in the TF group and 2.4% in the P-F group( P = 0.14). The grade 3/4 complications were similar between the two groups( P = 0.57). No differences in postoperative pancreatic fistula, abdominal infection or hemorrhage were observed between the two groups. Conclusions: The pancreatic “parenchyma transection-first” strategy is safe and feasible compared with traditional “tunnel-first strategy” in SPDP with Kimura’s procedure.

Karma Chameleon: Performative Acts, Gender Constitution, and the Second British Invasion

The aim is to examine the performative acts and gender constitution in the context of the Second British Invasion.Despite the pervasive character of patriarchy and the prevalence of sexual difference as an operative culturaldistinction, gender was not passively scripted on the bodies of many British singers. The subversive performancesdid not exclude suffering and marginalization but simultaneously undermined compulsory coherence.

Correlation of S100A13 and FOXA1 expression with cell cycle and cell invasion in fine needle aspiration thyroid carcinoma tissue

Objective: To study the correlation of S100A13 and FOXA1 expression with cell cycle and cell invasion in fine needle aspiration thyroid carcinoma tissue. Methods: Patients who received ultrasound-guided thyroid nodule fine needle aspiration in Haiyang People's Hospital between April 2015 and February 2017 were selected, and the tissues were divided into malignant thyroid tissue and benign thyroid nodules according to the pathological results after biopsy. The expression of S100A13, FOXA1, cell cycle molecules and cell invasion molecules were measured. Results: S100A13, FOXA1, CDK2, CyclinD1, MCM2, MCM7, SKP2, CLOCK, STAT3, STAT5, N-cadherin, MT1-MMP and ADAM17 mRNA expression in thyroid carcinoma tissue were significantly higher than those in benign thyroid nodule;CDK2, CyclinD1, MCM2, MCM7, SKP2 and CLOCK mRNA expression in thyroid carcinoma tissue with high FOXA1 expression were significantly higher than those in thyroid carcinoma tissue with low FOXA1 expression;STAT3, STAT5, N-cadherin, MT1-MMP and ADAM17 mRNA expression in thyroid carcinoma tissue with high S100A13 expression were significantly higher than those in thyroid cancer tissue with low S100A13 expression. Conclusions: High expression of S100A13 and FOXA1 in thyroid carcinoma can promote cell invasion and cell cycle progression.

GSPT1 Functions as a Tumor Promoter in Human Liver Cancer

Objective This study analyzed the role of G1 to S phase transition 1 protein(GSPT1)in promoting progression of liver cancer cells.Methods A bioinformatics database was used to analyze the expression levels of GSPT1 in liver cancer tissues and the prognosis of patients.Subsequently,Western blotting and quantitative PCR were used to verify the expression levels of GSPT1 between normal hepatocytes and hepatoma cells.We used a CRISPR/Cas9 system to construct knockouts of GSPT1 in HepG2 and HCCLM9 liver cancer cells.The effect of GSPT1 on liver cancer cell migration and invasion was analyzed using flow cytometry,migration,and tumor formation assays.Results The Cancer Genome Atlas Liver Hepatocellular Carcinoma dataset indicated that GSPT1 expression was upregulated in liver cancer cell lines,and patients with liver cancer had poor prognosis.Knockout of GSPT1 in cells significantly inhibited tumor proliferation,cell migration,and growth in vivo.Conclusion In this study,we found that GSPT1 promotes the migration and invasion of liver cancer cells.

Relationship between Prosopis juliflora invasion and livelihood diversification in the South Afar region,Northeast Ethiopia

This study assessed the effects of Prosopis juliflora invasion on livelihood diversification and evaluated the determinants of livelihood diversification in two districts in the South Afar region of Northeast Ethiopia.Using random sampling method,154 households and 2 focus groups composed of 10 individuals were selected from four sites in the two districts.The relationship between P.juliflora invasion and incomes obtained from P.juliflora varied across the study sites.In Awash Fentale District and Amibara District,11.0%and 3.2%of households,respectively,earned income from P.juliflora charcoal production.In total,14.3%of households were involved in charcoal production.In addition,19.9%of households earned income from selling P.juliflora pods and wood materials in the two districts.With the exception of income from P.juliflora charcoal production,the sex of households did not affect incomes from other livelihood activities.Moreover,household educational attainment significantly affected income obtained from vegetables,fruits,and cereal crops.Male householders were engaged in more diverse livelihood activities than female householders.Reversing the severe invasion of P.juliflora on grazing and woodlands in the region is advisable through integrated natural resources,pastoralist participation,and multidisciplinary research strategies.

Fournier’s gangrene after insertion of thermo-expandable prostatic stent for benign prostatic hyperplasia:A case report

BACKGROUND Thermo-expandable urethral stent(Memokath 028)implantation is an alternative treatment for older patients with lower urinary tract symptoms and benign prostatic obstruction.Following prostatic urethral stent implantation,minor complications such as urinary tract infection,irritative symptoms,gross hematuria,and urethral pain have been observed;however,there are no reports of life-threatening events.Herein,we report a critical case of Fournier’s gangrene that occurred 7 years after prostatic stenting.CASE SUMMARY An 81-years-old man with benign prostatic hyperplasia(volume,126 ccs;as measured by transrectal ultrasound)had undergone insertion of a thermoexpandable urethral stent(Memokath 028)as he was unfit for surgery under general anesthesia.However,the patient had undergone a suprapubic cystostomy for recurrent acute urinary retention 4 years after the insertion of prostatic stent(Memokath 028).We had planned to remove the Memokath 028;however,the patient was lost to follow-up.The patient presented to the emergency department 3 years after the suprapubic cystostomy with necrotic changes from the right scrotum to the right inguinal area.In digital rectal examination,tenderness and heat of prostate was identified.Also,the black skin color change with foulsmelling from right scrotum to right inguinal area was identified.In computed tomography finding,subcutaneous emphysema was identified to same area.He was diagnosed with Fournier’s gangrene based on the physical examination and computed tomography findings.In emergency room,Fournier’s gangrene severity index value is seven points.Therefore,he underwent emergent extended surgical debridement and removal of the Memokath 028.Broad-spectrum intravenous antibiotics were administered and additional necrotic tissue debridement was performed.However,the patient died 14 days after surgery due to multiorgan failure.CONCLUSION If Memokath 028 for benign prostatic hyperplasia is not working in older patients,its rapid removal may help prevent severe complications.

circHERC4通过调节miR-105-5p/Skp2轴抑制结直肠癌细胞的增殖、迁移和侵袭

目的 探究circHERC4对结直肠癌(CRC)细胞增殖、迁移和侵袭的影响与机制。方法 收集2020年6月~2021年12月在邵阳学院附属第一医院进行手术治疗的68例CRC病人的CRC组织和对应的癌旁组织。采用实时荧光定量PCR(qRT-PCR)检测组织中circHERC4、miR-105-5p、细胞S期激酶相关蛋白2(Skp2) mRNA表达水平;Western blot检测细胞中Skp2蛋白表达;CCK-8、平板细胞克隆实验、划痕愈合实验、Transwell实验分别检测细胞增殖、迁移、侵袭情况。结果 circHERC4、Skp2 mRNA在CRC组织中表达上调,而miR-105-5p表达下调(P<0.05),且CRC组织中circHERC4、miR-105-5p、Skp2 mRNA表达水平之间互呈相关性(P<0.05)。敲低circHERC4或过表达miR-105-5p可抑制HCT116细胞增殖、迁移和侵袭及细胞中Skp2表达(P<0.05),而过表达Skp2可部分逆转miR-105-5p过表达对HCT116细胞增殖、迁移和侵袭的抑制作用(P<0.05),且抑制miR-105-5p表达可部分逆转敲低circHERC4对HCT116细胞增殖、迁移和侵袭的抑制作用(P<0.05)。结论 敲低circHERC4可通过调节miR-105-5p/Skp2轴抑制HCT116细胞增殖、迁移和侵袭。

Extramammary Paget’s Disease Manifested by Intraepithelial Adenocarcinoma of the Vulva and Anus Combined with Invasive Adenocarcinoma of the Ampullary Part of the Rectum

The Object of the Study: The author of the given paper describes an unusual combination of two diseases: extramammary Paget’s disease manifested by intraepithelial adenocarcinoma of the vulva and anus combined with invasive adenocarcinoma of the ampullary part of the rectum and describes the atypical manifestations of these diseases. The Content: The content of this research paper includes a description of the patient, an analysis of the clinical picture, diagnostic methods and therapeutic interventions used, a report of the following disease, and the result of the presented case. The Result of the Research Work: The result of the research work is the analysis of a clinical case with two different tumors, where such a combination of tumors is rarely described in the literature. Moreover, no large specific sample with this combination of diseases is available. Patient Characteristics: The given case report describes a patient of the Palliative Care Unit of the Gerontology Clinic with a primary diagnosis of C20-rectal adenocarcinoma in the background of the anal canal, perineal skin Paget’s disease, stage IV. The presented complications of the patient’s primary diagnosis are multiple metastases in the liver;status post palliative chemotherapy;hepatomegaly;metastases to abdominal lymph nodes, inguinal lymph nodes;metastases at Th12, L4 level;pain syndrome. The presented above combination of diagnosed diseases is very rare. Applied Diagnostics: In October 2021, it was performed diagnostic manipulation: biopsy and the pathologist have provided a microscopic description. The first tissue fragment had a pronounced electrothermal lesion and the epithelial structures were not valuable. The second skin tissue fragment was covered with hyperplastic and acanthotic epithelium;its basal and middle layers contained multiple large cells proliferates extending into the medial epidermis, and the cytoplasm of these cells reacted positively with PAS (Periodic Acid Schiff reaction). It needs to be noted that the patient had previously had several years of biopsies from the perineal and anal epidermis, where Paget’s disease had also been diagnosed. The performed immunohistochemistry showed these cells to be CK20 positive, CK7 rare positive and p16 negative. The following pathohistological findings were made: morphological and immunohistochemical picture is consistent with Paget’s disease. According to the ICD-10, the patient was diagnosed with C51 malignant neoplasm of the female external genitalia. Using imaging diagnostics, it became clear that the patient’s rectal adenocarcinoma had progressed to metastatic stage with distant liver metastases in the background of anal canal, perineal skin Paget’s disease. Therapeutic Plan of the Patient: Based on the patient’s main diagnoses, the complications of the principal diagnosis, the patient’s overall severe condition, pain syndrome, age and comorbidities, palliative chemotherapy was approved as a therapeutic option in council of doctors. Monitoring and Outcome of the Patient: The patient’s general condition was becoming worse over time, and she was diagnosed with exitus latalis in December 2022. At that time, the patient was discharged from hospital and was on palliative care at home under the control of her family physician.

论伏邪在SARS发病中的作用

应用量表学的方法,制作调查问卷,并运用德尔菲法重新修订问卷结构和内容,进行一线专家大样本SARS中医证候及其演变规律的问卷调查。运用频数、决策树和神经网络明确证候因素的判断标准,归纳出主要证素。其中伏邪的证候因素在初期和极期较高,进入恢复前期和恢复后期依次递减。说明"伏邪致病"作为本病的证候因素是有其理论基础和科学依据的。

20(S)-原人参二醇衍生物对HT1080细胞侵袭转移的影响

检测了所合成的20(S)-原人参二醇衍生物GY1和GY2的抗肿瘤活性.GY1对HT1080、SMMC7721、A549的增殖有显著的抑制作用,呈明显的剂量依赖关系,IC50分别为:35.2±0.9 mg/L,29.6±1.1 mg/L,24.1±1.5 mg/L;而GY2并没有表现出的明显的细胞毒作用,其IC50大于100 mg/L.5 mg/L GY1作用48 h可显著降低HT1080细胞对基底膜成分的粘附和侵袭能力,并对细胞的运动能力有一定影响;而相同浓度的GY2虽然对细胞的粘附性和侵袭性有一定影响,但对细胞的运动性却无明显的影响.