Suppressed effects of Phyllanthus urinaria L.ethyl acetate extract on hepatitis B virus both in vitro and in vivo

Background:Phyllanthus urinaria L.(P.urinaria)extract(PUE)has been used to inhibit hepatitis B virus(HBV).However,the underlying mechanism remains unclear.To investigate which PUE fractions and main components lead to against HBV and approach the relevant molecular mechanisms.Methods:P.urinaria was extracted with water,and then the decoction was extracted by petroleum ether,ethyl acetate,and n-butanol in turn.The HepG2.2.15 cell was treated with aqueous fraction,petroleum ether fraction,ethyl acetate fraction and n-butanol fraction,gallic acid(GA,C7H6O5)and corilagin(CL,C27H22O18),respectively.The medium was collected for hepatitis B surface antigen(HBsAg)and hepatitis B e antigen assays.Cell counting kit-8 method was used to identify cell proliferation.Also,the levels of cellular oxygen consumption,reactive oxygen species,and reduced glutathione were detected.The HBV modeling mice were treated with ethyl acetate fraction,entecavir and physiological saline,respectively.The serum was collected for HBsAg and inflammatory cytokines assays.Liver tissue metabolites were screened by LC-MS/MS method.Results:The ethyl acetate fraction(EAF)of P.urinaria could significantly inhibit HBV secretion in HepG2.2.15(P<0.05).Furthermore,two main constitutes in ethyl acetate fraction,GA and CL,could significantly inhibit HBV secretion and reduced cell proliferation(P<0.05).Also,GA and CL could increase cellular oxygen consumption,intracellular superoxide anions level,superoxide dismutase level and glutathione depletion.Compared with the Modeling group,EAF significantly decreased the expression levels of HBsAg,IL-1β,IFN-α(P<0.05).LC-MS/MS analysis results showed that EAF dramatically up-regulate hydroxyproline,maltotriose,betaine and down-regulate glutathione disulfide,taurocholate,taurochenodeoxycholate(P<0.05).Kyoto Encyclopedia of Genes and Genomes results show that the differential metabolites were mainly enriched in ATP-binding cassette transporters pathway.Conclusions:P.urinaria exhibits suppressed effects on HBV by modulating reactive oxygen species formation or metabolomics both in vitro and in vivo.These data indicate that P.urinaria may be an alternative therapeutic agent for the treatment of HBV-related hepatitis.

Phytochemistry and ethnomedicinal qualities of metabolites from Phyllanthus emblica L.:A review

Phyllanthus emblica or Indian gooseberry is an integrated part of Ayurvedic and Traditional Chinese Medicines.For several decades,the well-known ancient herb has been extensively utilized in traditional medicine to cure diseases like fever,diabetes,constipation,jaundice,ulcers,biliousness,anemia,anorexia,and dyspepsia.In the traditional system,Indian gooseberry has various ethnomedicinal applications.In the Ayurvedic system,different methods of administration(anupan)have shown different ethnomedicinal properties of Indian gooseberry.Seventy well-known chemical components in Indian gooseberry have been identified through phytochemical evaluation,among which the flavonoids and phenols are most prominent.From the toxicity perspective,it is considered a safe herb in India,and is taken as a food supplement in European countries.The wide-spectrum pharmacological activities of the crude extracts and isolates of Indian gooseberry are attributed to the predominance of phenols and flavonoids.Thus,it is important to study the exact mechanism of the activity of the phytochemicals in Indian gooseberry,especially in anti-cancer activities.Extract of Indian gooseberry enhances proliferation in several cancer cells in vitro,including stem cells like ovarian cancer(OC)cells,and also has been observed to possess anti-proliferative characteristics in vivo.This review intends to explore the therapeutic potential of Indian gooseberry based on scientific reports and attempts to find the gaps for future research.

Network pharmacology and experimental validation to reveal the anti-hepatitis B virus pharmacological mechanism of Phyllanthus urinaria L.

Background:To explore the pharmacological mechanism of the anti-hepatitis B virus of Phyllanthus urinaria L.through network pharmacological analysis and experimental validation.Method:The active ingredient,target of action and target of action related to hepatitis B were clarified by searching the herb group identification,GeneCards and OMIM databases,and the protein interaction relationship was obtained by using the String database,and the protein interaction network map was constructed by using Cytoscape software.We also performed gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of key targets of the anti-hepatitis B action of Phyllanthus urinaria L.and predicted the core targets and pathways of Phyllanthus urinaria L.anti-hepatitis B.The main targets predicted by network pharmacology were then validated by HepG2.2.15 cell experiments.Results:By searching active ingredient targets and hepatitis B disease targets,a total of 19 active ingredients and 64 related targets of action were retrieved from Phyllanthus urinaria L.,and a total of 51 common targets were obtained by mapping the obtained hepatitis B disease targets and drug targets.protein protein interaction network analysis indicated that targets including TNF,JUN,AKT1,IL-10,IL-1B,CAT,HMOX1,NFE2L2,and CASP3 and other targets may be the core targets.gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that the treatment of hepatitis B by Phyllanthus urinaria L.mainly included inflammation and oxidation-related processes,and the signaling pathways mainly included fluid shear stress and atherosclerosis,VEGF,and hepatocellular carcinoma.The results of the in vitro test showed that after the action of different concentrations of the extracts of the Phyllanthus urinaria L.in the safe concentration range on cells HepG2.2.15,HBsAg,HBeAg and hepatitis B virus DNA levels were significantly inhibited,and NFE2L2 and HMOX1 were affecting hepatitis B virus transcription and replication by regulating the oxidative stress response.Conclusion:Using an integrated network pharmacology approach,this study revealed the active components and potential targets of Phyllanthus urinaria L.for the treatment of the hepatitis B virus,providing a theoretical basis for the research and clinical application of Phyllanthus urinaria L..

Two pyrrole acids isolated from Phyllanthus emblica L.and their bioactivities

An undescribed pyrrole acid,1-(4′-methoxy-4′-oxobutyl)-1 H-pyrrole-2,5-dicarboxylic acid(1)and one known pyr-role acid(2)were isolated from the fruits of Phyllanthus emblica.The structures of these compounds were elucidated via the comprehensive analyses of IR,HRESIMS,1D and 2D spectroscopic data.A series of biological assays revealed that compounds 1 and 2 could inhibit LPS-induced over-production of nitric oxide(NO),interleukin-6(IL-6),monocyte chemotactic protein 1(MCP-1)and tumor necrosis factor-α(TNF-α)by reducing the phosphorylation of extracellular regulated protein kinases(ERK)and c-Jun N-terminal kinases(JNK)in RAW 264.7 cells.Additionally,compounds 1 and 2 were found to reduce lipid deposition and increase the mRNA expression of ATP-binding cassette transporter A1 in oxidized low-density lipoprotein-treated RAW264.7 macrophages.

余甘子斑点病病原菌鉴定、生物学特性及防治药剂筛选

【目的】明确余甘子果实斑点病的病原菌种类,了解其生物学特性,筛选有效的防治药剂,为余甘子果实斑点病的科学诊断和田间防治提供理论依据。【方法】从广东省汕尾市陆河县余甘子产业园种植基地采集发生斑点病的余甘子果实,采用组织分离法对余甘子斑点病病果进行病原菌分离,通过形态学特征和基于多基因(ITS、TUB和EF1-α)的系统发育进化分析对病原菌进行鉴定;采用针刺接种法测定病原菌菌株的致病力;采用菌丝生长速率法测定不同培养条件对病原菌菌丝生长的影响;通过室内毒力测定评估12种常见杀菌剂对病原菌的抑菌活性。【结果】从余甘子病果中分离出1株病原真菌菌株PE3,致病性测定结果表明,该菌株为余甘子斑点病致病菌,其形态特征与间座壳菌Diaporthe phoenicicola相似,且其ITS、TUB和EF1-α基因系统发育进化树分析显示与D.phoenicicola聚在同一分支。生物学特性测定结果显示,菌株PE3菌丝生长的最适温度为25℃,最适p H为7,最适生长碳源为甘油和蔗糖,最适生长氮源为酵母提取物。室内毒力测定结果显示,450 g/L咪鲜胺乳油对菌株PE3菌丝生长的抑制作用最强,抑制中浓度(EC_(50))为0.019 mg/L;其次是8%氟硅唑微乳剂、10%苯醚甲环唑微乳剂和25%吡唑醚菌酯悬浮剂,EC_(50)分别为0.023、0.124和0.194 mg/L;而2%春雷霉素水剂对菌株PE3菌丝生长的抑制效果最差,EC_(50)为402.336 mg/L。【结论】间座壳菌D.phoenicicola是引起余甘子果实斑点病的病原菌,450 g/L咪鲜胺乳油、8%氟硅唑微乳剂、10%苯醚甲环唑微乳剂和25%吡唑醚菌酯悬浮剂能有效防治余甘子果实斑点病,可在余甘子种植中推广应用。

叶下珠黄化植原体和丛枝植原体的分子鉴定

2022年8月在海南省文昌市一个槟榔黄化病园内,分别发现叶片黄化和丛枝症状的叶下珠,前期检测均为植原体感染。为了明确叶下珠黄化植原体和丛枝植原体的分类鉴定,本研究通过克隆16S rDNA基因和核糖体蛋白(rp)基因,并进行基因序列一致性、系统发育树及虚拟RFLP等分析。结果显示:克隆获得叶下珠黄化植原体16S rDNA片段1246 bp,rp基因1212 bp;叶下珠丛枝植原体16S rDNA片段1827 bp,rp基因1240 bp。基因序列一致性显示,叶下珠黄化植原体与丛枝植原体的16S rDNA基因序列均与16SrⅠ组的植原体一致性高达98%以上,与槟榔黄化植原体海南株系的16S rDNA序列一致性达100%;而二者的rp基因序列均与rpⅠ组的植原体一致性高达99%以上。系统发育树分析显示,叶下珠黄化植原体和丛枝植原体16S rDNA基因均与翠菊黄化组(16SrⅠ)植原体聚于一个大分支,且与16SrⅠ-B亚组的槟榔黄化植原体聚于同一个小分支,亲缘关系接近;而rp基因均与翠菊黄化组(rpⅠ)植原体集聚于一个大分支,且与rpⅠ-B亚组的翠菊黄化植原体聚于同一个小分支,亲缘关系接近。虚拟RFLP分析显示,叶下珠黄化植原体和丛枝植原体的16S rDNA基因序列虚拟RFLP图谱与16SrⅠ-B的洋葱黄化植原体的参考图谱相同,且相似系数为1.00。综上表明,叶下珠黄化植原体和丛枝植原体均属于16SrⅠ-B亚组成员。研究结果可对采用铲除槟榔黄化病中间寄主的防控手段提供理论依据。

Screening of Anti-Newcastle Disease Virus Ingredients of Phyllanthus urinaria

[ Objective ] The aim of the study was to screen the antiviral ingredients of Chinese herbal medicine Phyllanthus urinaria in order to devel- op new drugs for the treatment of viral diseases of poultry. [ Method ] The whole grass of phyllanthus was respectively decocted in 75% ethanol （E） and pure water （PF）, and then the active ingredients were separately extracted in petroleum ether （PE）, ethyl acetate （EA）, and n-butyl alcohol （BU）. The extracts were inoculated on chicken embryo fibroblast （CEF） to observe their inhibitory effect on cytopathic effect （CPE） of Newcastle disease virus （NDV）, inoculated into chicken embryos to observe the changes of the hemagglutination titer of NDV, and inoculated in chickens to determine the mortality and evaluate their effect of immune protection. [ Result] The E -PE and E -BU extracts, especially E -BU extract, inhibi- ted CPE of NDV on CEF and significantly inhibited the proliferation of NDV in chicken embryo （P〈0.05） ; the survival rate of E-PE and E-BU group was extremely significantly higher than that of ribavirin group and the normal saline control （ P 〈0.01 ）, with E - PE group more higher. [ Condusion] The water extract of phyllanthus is less effective; E -PE extract and E -BU extract should effectively inhibit the CPE and proliferation in chicken embryo of NDV and also have better immune protection against NDV.

余甘子(Phyllanthus emblica L.)Vc含量分析

采用2,6-二氯酚靛酚滴定法分析比较了余甘子各器官之间、同品种不同植株之间、不同余甘子品种之间以及与野生资源之间的Vc含量以及不同预处理方法对Vc测定的影响。结果表明,Vc在余甘子各器官中均有分布,以果实与初生幼叶中居多,茎、根中含量较低;皇帝甘与粉甘含量较多,但与其它品种的差异不大;5个野生资源单株Vc含量差异极显著,其中有3个野生单株的果肉Vc含量高于主栽品种粉甘。

余甘子提取物与四种中药提取物复配的促消化效果的研究

通过小鼠小肠运动、胃排空、大鼠摄食量、体重增加、食物利用率、胃蛋白酶测定等实验对比余甘子提取物分别与四种提取物(陈皮、茯苓、山楂及白术)两两复配(两两复配产品),及五种提取物复配产品的促消化效果。结果表明两两复配产品及五种提取物复配产品均能显著提高小鼠小肠推进率,并能显著提高其胃排空率;五种提取物复配产品组较空白组显著提升大鼠食物利用率(P<0.05);余甘子山楂复配产品组,余甘子茯苓复配产品组及五种提取物复配产品组相较空白组大鼠的胃蛋白酶活性都有明显提升(P<0.05);除了余甘子陈皮复配提取物外,相较空白组,其他两两复配产品组均有提升大鼠胃蛋白酶排出量的效果(P<0.05)。其作用可能与提高胃肠动力及提高胃蛋白酶活力相关,本研究为余甘子等复配提取物改善胃肠消化功能提供理论依据,并为研发提高胃肠动力的产品提供思路。

超声辅助不同小分子糖渗透处理后真空冷冻干燥油柑的品质比较

该研究评价了超声辅助五种小分子糖渗透处理后冻干油柑的理化特性及体外抗氧化活性。结果表明:相比对照组,经超声辅助渗糖处理的油柑水分损失率、固形物增加率升高;冻干油柑内部孔隙更加致密,硬度显著提高、脆度基本保持,复水性下降;低聚异麦芽糖处理组的吸湿率(18.25%)低于对照组(22.54%);葡萄糖和麦芽糖处理组的冻干油柑色差ΔE小,分别为2.73和1.71,即颜色保留率高;低聚麦芽糖和蔗糖对冻干油柑的风味强化效果较好;蔗糖、葡萄糖和麦芽糖处理组的冻干油柑可滴定酸含量较低;与对照组玻璃化转变温度(T_(g)=43.66℃)相比,麦芽糖处理组的T_(g)升高到45.53℃,其他处理组则显著降低了样品的T_(g);除木糖醇处理组(9.44 mg/g)外,不同超声渗糖处理组的总酚保留率均较高;蔗糖和低聚异麦芽糖渗透处理可有效保持冻干油柑的体外抗氧化能力。总的来说,超声辅助蔗糖和低异聚麦芽糖渗透处理的冻干油柑品质较好,其中低异聚麦芽糖可作为一种蔗糖替代品用于开发功能性冻干油柑。

3个余甘子优良无性系在广西贵港市种植表现及品质特性分析

为筛选出适合在广西贵港市种植的余甘子优良无性系,为余甘子良种培育和生产提供科学依据。以野生余甘子为对照,调查和分析“平丹一号”“平丹二号”“平丹三号”3个余甘子优良无性系在广西贵港市的种植表现及品质特性。结果表明,“平丹一号”和“平丹二号”在广西贵港市表现为早熟或中早熟,“平丹三号”为中熟;3个余甘子优良无性系丰产性较好,5年生树单株鲜果产量在46.10~50.95 kg之间,单果质量在9.32~10.54 g之间,都显著高于野生余甘子。不同余甘子优良无性系果实可溶性固形物含量在10.60%以上,果实单宁、维生素C、可溶性糖、可溶性蛋白等内含物含量较高。“平丹一号”“平丹二号”和“平丹三号”在广西贵港市综合表现较好,抗病性较强,可选择培育成良种并加以推广。

余甘子水提物对黑腹果蝇寿命及攀爬能力的保护作用

目的:余甘子(PE)是一种富含多种酚类物质和有机酸等化合物的药食两用植物资源。本研究拟探讨PE水提物对正常生理条件和镉(Cd)胁迫条件下果蝇寿命及攀爬能力的影响。方法:采用1.6、8、16 mg/mL PE水提物分别作用于野生型雌雄红黑腹果蝇,寻找有效提高其寿命及攀爬能力的最佳PE水提物浓度和最佳处理时间,并检测最佳浓度PE水提物处理后对雌雄果蝇体内SOD、CAT活性、MDA含量的影响。用5、10、20、40 mg/L CdCl_(2)单独作用于雌雄果蝇,摸索有效降低果蝇寿命的最适CdCl_(2)浓度,在此基础上采用1.6、8、16 mg/mL PE水提物联合最适浓度CdCl_(2)处理雌雄果蝇,探讨PE水提物对Cd胁迫下雌雄果蝇寿命及攀爬能力的影响。结果:与对照组相比,16 mg/mL PE水提物有助于延长正常生理条件下雌雄果蝇寿命(P<0.05);在最佳浓度(雌性1.6 mg/mL、雄性8 mg/mL)PE水提物处理下,有效提高雌雄果蝇攀爬能力的最佳处理时间呈现出性别差异,雌果蝇的最佳处理时间为22 d,雄果蝇为10 d,但最佳浓度PE水提物分别处理雌雄果蝇10、22、33、45 d均不能提高其体内抗氧化酶活性。与对照组相比,40 mg/L CdCl_(2)作用下雌雄果蝇的平均寿命降低最多(P<0.01),后续以40 mg/L作为最适Cd胁迫浓度。与对照组比较,8和16 mg/mL PE水提物有助于延长40 mg/L CdCl_(2)胁迫下雄果蝇的寿命但处理18 d后其攀爬能力显著降低(P<0.05),1.6、8和16 mg/mL PE水提物处理对雌果蝇的寿命和攀爬能力则均无显著影响(P>0.05)。结论:PE水提物有助于延长正常生理条件下雌雄果蝇的寿命和攀爬能力,延长Cd胁迫下雄果蝇的寿命但降低其攀爬能力,表明PE对果蝇的保护作用存在性别差异。

云南野生余甘子鞣花酸和漆黄素的减肥降脂作用

余甘子(Phyllanthus emblica,PE)是近年广受关注的药食两用水果,但关于其减肥降脂功效及具体作用成分鲜有报道。该研究提取了云南野生PE富含的黄酮多酚物质(Phyllanthus Emblica Extract,PEE),发现其在小鼠前脂肪细胞(3T3-L1)模型中,能够显著抑制3T3-L1细胞分化至成熟脂肪细胞,减少脂滴生成量,300μg/mL作用时,细胞总甘油三酯(Triglyceride,TG)、总胆固醇(Total Cholesterol,TC)相对含量分别降低至66.47%、40.87%,这表明,云南野生PE中的PEE具有减肥降脂潜力。通过HPLC分析测得,云南野生PE中含有3030.78μg/g鞣花酸,560.70μg/g漆黄素。将鞣花酸和漆黄素以5:1的比例还原搭配成混合物(Ellagic Acid and Fisetin,EF),研究其对高脂饮食(High Fat Diet,HFD)小鼠模型的影响。结果显示,EF能够抑制HFD小鼠的体质量和白色脂肪增长,高剂量干预时,血清TG、TC、LDL-C含量分别降低了45.48%、12.06%、37.54%,HDL-C水平提高了18.75%;HFD小鼠的肝脏指数、血清中ALT和AST分别降低了7.80%、21.59%、41.64%。EF具有减肥降脂功效,且对肝脏具有一定的修复作用,是云南野生PE减肥降脂作用的重要成分。

热带地区叶下株内生菌的分离筛选和抑菌活性研究

为挖掘具有生物学活性的内生菌资源,给临床新型药物研究提供更多的筛选源,采用组织分离技术和划线法,从海南地区的新鲜叶下珠中分离纯化内生菌,通过试管梯度稀释法测定内生菌对大肠埃希菌、金黄色葡萄球菌、白假丝酵母菌等常见病原菌的抑菌活性。结果显示,从叶下珠中分离得到13株菌,其中YXZ-10对大肠埃希菌具有良好的抑菌效果,说明海南地区叶下株内生菌资源丰富,部分具有抑菌活性。

余甘(Phyllanthus emblica L.)大小孢子发生及雌雄配子体发育研究

采用常规石蜡切片技术,对平丹1号余甘大小孢子发生及雌雄配子体发育进行研究。结果表明:药壁发育为双子叶型;绒毡层为腺质绒毡层;小孢子母细胞减数分裂时胞质分裂为同时型;产生四面体形四分体;成熟的花粉粒为二细胞型花粉;平丹1号余甘的雌蕊为上位子房,3心皮3室,每室含倒生胚珠2枚,为厚珠心,具珠心喙,胚囊为蓼形;平丹1号余甘部分大孢子母细胞、大孢子及胚囊发育异常,致使不能形成正常的卵细胞,不能进行受精。

余甘子提取物及其复配物改善大鼠的功能性消化不良症状

该研究通过《保健食品功能检验与评价方法(2022年版)》中的促进消化功能检验方法中小鼠小肠运动、大鼠增重、摄食量、食物利用率、胃蛋白酶测定等试验研究了余甘子单方和四种复方的促消化效果。结果表明,余甘子(单方组),余甘子、山楂、茯苓和陈皮(复方1组),余甘子、山楂、白术和麦芽(复方2组),余甘子、山楂、茯苓、白术和陈皮(复方3组),余甘子、山楂、茯苓、白术、麦芽和陈皮(复方4组)小鼠小肠推进率分别提高了65.99%、80.53%、107.91%、67.47%、90.47%(P<0.05,P<0.01,P<0.0001,P<0.05,P<0.001),大鼠胃蛋白酶活性分别增强了89.24%、60.42%、103.07%、74.56%、106.84%(P<0.01,P≥0.05,P<0.01,P<0.05,P<0.01),大鼠胃蛋白酶排出量分别增强了89.30%、60.39%、103.14%、74.69%、106.98%(P<0.01,P≥0.05,P<0.01,P<0.05,P<0.01)。此外,复方4组大鼠食物利用率增加了15.35%(P<0.05),体质量增长、摄食量无显著差异(P≥0.05),其他组别的上述3项指标均无显著影响(P≥0.05)。研究表明余甘子提取物及其复配物可有效改善大鼠功能性消化不良症状。该研究为余甘子及其复配物促消化功能产品的开发和推广提供了相应的理论参考。

余甘果酱的研制及其体外降脂功效研究

以余甘果、山楂为原料,辅以一定的荷叶、葛根、薏苡仁浸提液,研制一种具有降脂功效的余甘果酱。以感官评价、黏度、色差为评价指标,采用单因素及正交试验,确定余甘果酱的最佳配方,并研究其体外降脂功效。结果表明:以余甘果和山楂总质量为基准,余甘果酱最佳参数为余甘果和山楂质量比97∶3、木糖醇添加量30%、蜂蜜添加量22%、黄原胶添加量0.4%。体外降脂功效表明:余甘果酱对花生油的吸附量为9.82 mg/g,对胰脂肪酶能起一定的抑制作用,且随着浓度的增加抑制效果显著增大;余甘果酱在pH 7.0条件下对胆固醇的吸附量达176.08 mg/g。余甘果酱酸甜可口,有较好的降脂功效。

余甘子多酚提取工艺及测定方法研究进展

余甘子是我国常用的药食同源植物,多酚类成分是余甘子的主要活性成分之一。现代药理学研究表明,余甘子多酚具有抗氧化、抗炎、抗病毒、抗肿瘤及保肝护肝等作用。因此,余甘子中多酚类成分的高效提取及含量测定研究对于深度开发利用余甘子及其相关功能保健产品开发具有重要意义。本文对近年来关于余甘子多酚的提取工艺及测定方法的研究进行综述,以期为余甘子多酚的进一步开发利用提供参考依据。

基于模糊数学和响应面法优化余甘子果汁饮料生产工艺

以新鲜余甘子果实为原料制备余甘子果汁饮料。在单因素试验的基础上,以综合得分为指标,通过正交试验优化纤维素酶酶解工艺;再在单因素试验的基础上,以综合感官评分为指标,通过响应面法优化余甘子果汁饮料配方。结果表明:最佳酶解工艺为纤维素酶添加量0.15%(以余甘子原汁质量计)、酶解温度55℃、酶解时间40 min;最佳饮料配方为以余甘子果汁饮料质量为基准,余甘子原汁添加量30%、白砂糖添加量11%、食盐添加量0.13%、柠檬酸添加量0.18%,在此条件下制得的余甘子果汁饮料色、香、味佳,综合感官评分为88.10。

叶下珠饮片质量标准优化研究

目的:优化叶下珠饮片的质量标准。方法:采用薄层色谱(TLC)法对叶下珠饮片进行定性鉴别;采用电子显微镜观察叶下珠饮片粉末及横切面特征;采用高效液相色谱法测定没食子酸、柯里拉京的含量;参照2020年版《中华人民共和国药典》测定叶下珠饮片中水分、总灰分、酸不溶灰分和醇溶性浸出物的含量。结果:TLC分析中,叶下珠饮片与对照药材在相近位置均显示紫红色斑点。显微鉴别中,饮片粉末、横切面所含各种组织结构均可作为鉴别依据。含量分析中,没食子酸的线性回归方程为Y=15068X-14509(r=0.9998),含量为0.0110%~0.2582%;柯里拉京的线性回归方程为Y=38265X-7032(r=0.9999),含量为0.1267%~0.3291%;水分、总灰分、酸不溶灰分及醇溶性浸出物含量分别为9.49%~11.29%、6.02%~9.92%、0.64%~1.67%、15.73%~30.25%。结论:该质量标准可为准确、全面控制叶下珠饮片质量提供技术支持。