Screening of Anti-Newcastle Disease Virus Ingredients of Phyllanthus urinaria

[ Objective ] The aim of the study was to screen the antiviral ingredients of Chinese herbal medicine Phyllanthus urinaria in order to devel- op new drugs for the treatment of viral diseases of poultry. [ Method ] The whole grass of phyllanthus was respectively decocted in 75% ethanol （E） and pure water （PF）, and then the active ingredients were separately extracted in petroleum ether （PE）, ethyl acetate （EA）, and n-butyl alcohol （BU）. The extracts were inoculated on chicken embryo fibroblast （CEF） to observe their inhibitory effect on cytopathic effect （CPE） of Newcastle disease virus （NDV）, inoculated into chicken embryos to observe the changes of the hemagglutination titer of NDV, and inoculated in chickens to determine the mortality and evaluate their effect of immune protection. [ Result] The E -PE and E -BU extracts, especially E -BU extract, inhibi- ted CPE of NDV on CEF and significantly inhibited the proliferation of NDV in chicken embryo （P〈0.05） ; the survival rate of E-PE and E-BU group was extremely significantly higher than that of ribavirin group and the normal saline control （ P 〈0.01 ）, with E - PE group more higher. [ Condusion] The water extract of phyllanthus is less effective; E -PE extract and E -BU extract should effectively inhibit the CPE and proliferation in chicken embryo of NDV and also have better immune protection against NDV.

Network pharmacology and experimental validation to reveal the anti-hepatitis B virus pharmacological mechanism of Phyllanthus urinaria L.

Background:To explore the pharmacological mechanism of the anti-hepatitis B virus of Phyllanthus urinaria L.through network pharmacological analysis and experimental validation.Method:The active ingredient,target of action and target of action related to hepatitis B were clarified by searching the herb group identification,GeneCards and OMIM databases,and the protein interaction relationship was obtained by using the String database,and the protein interaction network map was constructed by using Cytoscape software.We also performed gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of key targets of the anti-hepatitis B action of Phyllanthus urinaria L.and predicted the core targets and pathways of Phyllanthus urinaria L.anti-hepatitis B.The main targets predicted by network pharmacology were then validated by HepG2.2.15 cell experiments.Results:By searching active ingredient targets and hepatitis B disease targets,a total of 19 active ingredients and 64 related targets of action were retrieved from Phyllanthus urinaria L.,and a total of 51 common targets were obtained by mapping the obtained hepatitis B disease targets and drug targets.protein protein interaction network analysis indicated that targets including TNF,JUN,AKT1,IL-10,IL-1B,CAT,HMOX1,NFE2L2,and CASP3 and other targets may be the core targets.gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that the treatment of hepatitis B by Phyllanthus urinaria L.mainly included inflammation and oxidation-related processes,and the signaling pathways mainly included fluid shear stress and atherosclerosis,VEGF,and hepatocellular carcinoma.The results of the in vitro test showed that after the action of different concentrations of the extracts of the Phyllanthus urinaria L.in the safe concentration range on cells HepG2.2.15,HBsAg,HBeAg and hepatitis B virus DNA levels were significantly inhibited,and NFE2L2 and HMOX1 were affecting hepatitis B virus transcription and replication by regulating the oxidative stress response.Conclusion:Using an integrated network pharmacology approach,this study revealed the active components and potential targets of Phyllanthus urinaria L.for the treatment of the hepatitis B virus,providing a theoretical basis for the research and clinical application of Phyllanthus urinaria L..

Suppressed effects of Phyllanthus urinaria L.ethyl acetate extract on hepatitis B virus both in vitro and in vivo

Background:Phyllanthus urinaria L.(P.urinaria)extract(PUE)has been used to inhibit hepatitis B virus(HBV).However,the underlying mechanism remains unclear.To investigate which PUE fractions and main components lead to against HBV and approach the relevant molecular mechanisms.Methods:P.urinaria was extracted with water,and then the decoction was extracted by petroleum ether,ethyl acetate,and n-butanol in turn.The HepG2.2.15 cell was treated with aqueous fraction,petroleum ether fraction,ethyl acetate fraction and n-butanol fraction,gallic acid(GA,C7H6O5)and corilagin(CL,C27H22O18),respectively.The medium was collected for hepatitis B surface antigen(HBsAg)and hepatitis B e antigen assays.Cell counting kit-8 method was used to identify cell proliferation.Also,the levels of cellular oxygen consumption,reactive oxygen species,and reduced glutathione were detected.The HBV modeling mice were treated with ethyl acetate fraction,entecavir and physiological saline,respectively.The serum was collected for HBsAg and inflammatory cytokines assays.Liver tissue metabolites were screened by LC-MS/MS method.Results:The ethyl acetate fraction(EAF)of P.urinaria could significantly inhibit HBV secretion in HepG2.2.15(P<0.05).Furthermore,two main constitutes in ethyl acetate fraction,GA and CL,could significantly inhibit HBV secretion and reduced cell proliferation(P<0.05).Also,GA and CL could increase cellular oxygen consumption,intracellular superoxide anions level,superoxide dismutase level and glutathione depletion.Compared with the Modeling group,EAF significantly decreased the expression levels of HBsAg,IL-1β,IFN-α(P<0.05).LC-MS/MS analysis results showed that EAF dramatically up-regulate hydroxyproline,maltotriose,betaine and down-regulate glutathione disulfide,taurocholate,taurochenodeoxycholate(P<0.05).Kyoto Encyclopedia of Genes and Genomes results show that the differential metabolites were mainly enriched in ATP-binding cassette transporters pathway.Conclusions:P.urinaria exhibits suppressed effects on HBV by modulating reactive oxygen species formation or metabolomics both in vitro and in vivo.These data indicate that P.urinaria may be an alternative therapeutic agent for the treatment of HBV-related hepatitis.

Progress in Pharmacological Research of Phyllanthus urinaria

Phyllanthi Urinariae Herba is dry grass of Phyllanthus urinaria,and has the effects of calming liver,clearing heat,diuresis and detoxification in traditional Chinese medicine. Studies have shown that the effects of anti-hepatitis B virus,hepatoprotective,anti-tumor,antipathogenic microorganism,anti-oxidation,and anti-thrombosis were good and worthy of further research and development. The research progress on the pharmacological effects of P. urinaria was reviewed in order to provide a reference for the rational development of this product.

Ideas and Recommendations for Development of Gauri Gvong Phyllanthus embica Linn Industry

Gauri Gvong Phyllanthus embica Linn is fine variety domesticated by Lujiang Town farmers in Longyang District through many years. It has high economic and ecological benefits and is a medicinal and edible fruit in hot-dry valley regions. Gauri Gvong Phyllanthus embica Linn planting ranks the first place in Baoshan City and even in the whole Yunnan Province. It introduced current situations of Gauri Gvong Phyllanthus embica Linn industry in Longyang District and came up with development ideas,objectives and recommendations.

Neuroprotective effect of Phyllanthus urinaria extract on ischemic brain injury in rats

Objective:To investigate the neuroprotective effect of Phyllanthus urinaria on ischemic stroke and its primary mechanism.Methods:Adult SD rats were selected as the research object,and the right middle cerebral artery infarction rat model was established by the modified suture method(MCAO).Observe the neurological deficit score at 24h,48h and 72h after the model is successfully prepared,then TTC staining method to detect the area of cerebral infarction,the content of superoxide dismutase(SOD),nitric oxide(NO)and endothelial NOS(eNOS)in brain tissue;Immunofluorescence method was used to detect the expression of Caspase-3 positive cells in brain tissue;Western blot method was used to detect the expression of PI3K and AKT protein in brain tissue.72 experimental animals were randomly divided into 4 groups,sham operation group,model group(MCAO),extract of Phyllanthus urinaria low dose group(PuL5 g/kg),high dose group(PuL10 g/kg),and make MCAO model after 7 days of continuous administration,and continue to infuse the medicine once/d until the material is obtained.Results:No neurological deficits in the sham operation group,The 24h,48h and 72h of the modelling showed that the neurological impairment of the two doses of extract of Phyllanthus urinaria and the MCAO group was more severe than that of the sham operation group(P<0.01),however,with the prolongation of the modeling time,the neurological function scores of the two doses of extract of Phyllanthus urinaria were lower than those of the MCAO group,the most significant at 72h(P<0.01);The infarct size of the rats in the two dose groups of extract of Phyllanthus urinaria was lower than that of the MCAO group(P<0.01),and there was no dose dependence between the two groups,the content of SOD in the MCAO group was reduced,and the content of NO and eNOS was increased than the sham operation group(P<0.05),Compared with the MCAO group,the two administration groups significantly increased the content of SOD,decreased the content of NO and eNOS(P<0.05);Although the expression of Caspase-3 positive cells in the two administration groups was higher than that in the sham operation group,it was significantly lower than that in the MCAO group(Plow<0.05,Phigh<0.01);The expression of PI3K and AKT protein in the brain tissue of the MCAO group was significantly lower than that of the sham operation group(P<0.05),but the expression of PI3K and AKT protein in brain tissue of extract of Phyllanthus urinaria in high and low dose groups was significantly higher than that in MCAO group(P<0.05).Conclusion:Extract of Phyllanthus urinaria can improve neurological damage and cerebral infarction area in rats,and reduce the expression of Caspase-3 positive cells in MCAO rats,and then increase the expression levels of PI3K and AKT proteins to protect ischemic brain injury.

The anti-HIV-1 activity of polyphenols from Phyllanthus urinaria and the pharmacokinetics and tissue distribution of its marker compound, gallic acid

Objective:To investigate the in vitro anti-HIV-1 activities and its associated mechanism of action of an extract isolated from Phyllanthus urinaria (P.urinaria) and to develop an HPLC test method for detecting gallic acid (GA) in plasma and tissues to study its pharmacokinetics and tissue distribution in rats.Methods:An extract of P.urinaria was isolated and purified by phytochemistry and chromatography techniques.The anti-HIV-1 activities and toxicities of the extract and its component GA were determined in human T lymph cells (MT-4) by theMTTr method.The mechanism of its anti-HIV-1 action was studied to examine the in vitro binding of its components with HIV-1 target proteins by Biacore technique.The pharmacokinetics and tissue distribution of GA were investigated after oral administration of polyphenol extract (PE) and pure GA in rats.The concentrations of GA in plasma and tissues were determined by HPLC.Results:The PE and GA isolated from P.urinaria had anti-HIV-1 activities with IC50s of 0.61 μg/mL and 0.76 μg/mL,respectively.The Biacore study indicated that PE and GA interacted with HIV-1 RT,gp120,and P24.The pharmacokinetic parameters Tmax,Cm ax,AUC0-t,and T1/2 for GA were (60.0 ± 3.0) minutes,(2.87 ± 0.50) μg·mL-1,(343.5 ± 11.2) mg·min·L-1,and (113.3 ± 9.3) minutes while the parameters for GA in the PE were (10.0 ± 1.3) minutes,(3.89 ± 0.90) μg·mL-1,(394.7 ± 14.0) mg· min· L-1,and (81.7 ± 4.1) minutes,respectively.GA was detected in rat lungs,liver,kidneys,heart and spleen.Conclusion:APE isolated from P.urinaria containing GA has anti-HIV-1 activities.GA is quickly absorbed and slowly eliminated in rats after oral administration.The pharmacokinetics of GA administered as a PE is desirable,and it is widely distributed in the main tissues of lung and liver.Both its properties and anti-HIV-1 activities make it of interest for further studies.

Development of species-specific SCAR markers for identification of three medicinal species of Phyllanthus

Phyllanthus amarus Schum. & Thonn. has been widely used in traditional medicine in Thailand as an antipyretic, a diuretic, to treat liver diseases and viral infections. Two closely related species, P. debilis L. and P. urinaria Klein ex Willd., with different and less effective medicinal properties, are less commonly used. These three species are similar in morphology and often occur in overlapping populations in nature. The latter two species can easily be mistaken for P. amarus and collected for medicinal uses, which can lead to undesirable results. DNA fingerprints of these species were obtained using RAPD-PCR techniques. RAPD markers specific for each species were identified. Primers for highly specific sequence-characterized-amplified-regions (SCAR) were then designed from nucleotide sequences of specific RAPD markers. These primers efficiently amplified SCAR markers of 408, 501 and 319 bp unique to P. amarus, P. debilis and P. urinaria respectively. This method of plant identification was rapid and highly specific when tested against DNA of several closely related species and was able to amplify specific markers from mixed DNA samples.

叶下珠黄化植原体和丛枝植原体的分子鉴定

2022年8月在海南省文昌市一个槟榔黄化病园内,分别发现叶片黄化和丛枝症状的叶下珠,前期检测均为植原体感染。为了明确叶下珠黄化植原体和丛枝植原体的分类鉴定,本研究通过克隆16S rDNA基因和核糖体蛋白(rp)基因,并进行基因序列一致性、系统发育树及虚拟RFLP等分析。结果显示:克隆获得叶下珠黄化植原体16S rDNA片段1246 bp,rp基因1212 bp;叶下珠丛枝植原体16S rDNA片段1827 bp,rp基因1240 bp。基因序列一致性显示,叶下珠黄化植原体与丛枝植原体的16S rDNA基因序列均与16SrⅠ组的植原体一致性高达98%以上,与槟榔黄化植原体海南株系的16S rDNA序列一致性达100%;而二者的rp基因序列均与rpⅠ组的植原体一致性高达99%以上。系统发育树分析显示,叶下珠黄化植原体和丛枝植原体16S rDNA基因均与翠菊黄化组(16SrⅠ)植原体聚于一个大分支,且与16SrⅠ-B亚组的槟榔黄化植原体聚于同一个小分支,亲缘关系接近;而rp基因均与翠菊黄化组(rpⅠ)植原体集聚于一个大分支,且与rpⅠ-B亚组的翠菊黄化植原体聚于同一个小分支,亲缘关系接近。虚拟RFLP分析显示,叶下珠黄化植原体和丛枝植原体的16S rDNA基因序列虚拟RFLP图谱与16SrⅠ-B的洋葱黄化植原体的参考图谱相同,且相似系数为1.00。综上表明,叶下珠黄化植原体和丛枝植原体均属于16SrⅠ-B亚组成员。研究结果可对采用铲除槟榔黄化病中间寄主的防控手段提供理论依据。

余甘子水提物对黑腹果蝇寿命及攀爬能力的保护作用

目的:余甘子(PE)是一种富含多种酚类物质和有机酸等化合物的药食两用植物资源。本研究拟探讨PE水提物对正常生理条件和镉(Cd)胁迫条件下果蝇寿命及攀爬能力的影响。方法:采用1.6、8、16 mg/mL PE水提物分别作用于野生型雌雄红黑腹果蝇,寻找有效提高其寿命及攀爬能力的最佳PE水提物浓度和最佳处理时间,并检测最佳浓度PE水提物处理后对雌雄果蝇体内SOD、CAT活性、MDA含量的影响。用5、10、20、40 mg/L CdCl_(2)单独作用于雌雄果蝇,摸索有效降低果蝇寿命的最适CdCl_(2)浓度,在此基础上采用1.6、8、16 mg/mL PE水提物联合最适浓度CdCl_(2)处理雌雄果蝇,探讨PE水提物对Cd胁迫下雌雄果蝇寿命及攀爬能力的影响。结果:与对照组相比,16 mg/mL PE水提物有助于延长正常生理条件下雌雄果蝇寿命(P<0.05);在最佳浓度(雌性1.6 mg/mL、雄性8 mg/mL)PE水提物处理下,有效提高雌雄果蝇攀爬能力的最佳处理时间呈现出性别差异,雌果蝇的最佳处理时间为22 d,雄果蝇为10 d,但最佳浓度PE水提物分别处理雌雄果蝇10、22、33、45 d均不能提高其体内抗氧化酶活性。与对照组相比,40 mg/L CdCl_(2)作用下雌雄果蝇的平均寿命降低最多(P<0.01),后续以40 mg/L作为最适Cd胁迫浓度。与对照组比较,8和16 mg/mL PE水提物有助于延长40 mg/L CdCl_(2)胁迫下雄果蝇的寿命但处理18 d后其攀爬能力显著降低(P<0.05),1.6、8和16 mg/mL PE水提物处理对雌果蝇的寿命和攀爬能力则均无显著影响(P>0.05)。结论:PE水提物有助于延长正常生理条件下雌雄果蝇的寿命和攀爬能力,延长Cd胁迫下雄果蝇的寿命但降低其攀爬能力,表明PE对果蝇的保护作用存在性别差异。

叶下珠饮片质量标准优化研究

目的:优化叶下珠饮片的质量标准。方法:采用薄层色谱(TLC)法对叶下珠饮片进行定性鉴别;采用电子显微镜观察叶下珠饮片粉末及横切面特征;采用高效液相色谱法测定没食子酸、柯里拉京的含量;参照2020年版《中华人民共和国药典》测定叶下珠饮片中水分、总灰分、酸不溶灰分和醇溶性浸出物的含量。结果:TLC分析中,叶下珠饮片与对照药材在相近位置均显示紫红色斑点。显微鉴别中,饮片粉末、横切面所含各种组织结构均可作为鉴别依据。含量分析中,没食子酸的线性回归方程为Y=15068X-14509(r=0.9998),含量为0.0110%~0.2582%;柯里拉京的线性回归方程为Y=38265X-7032(r=0.9999),含量为0.1267%~0.3291%;水分、总灰分、酸不溶灰分及醇溶性浸出物含量分别为9.49%~11.29%、6.02%~9.92%、0.64%~1.67%、15.73%~30.25%。结论:该质量标准可为准确、全面控制叶下珠饮片质量提供技术支持。

叶下珠的有效部位和药理作用研究进展

叶下珠是临床常用治疗癌症的中药材,其发挥作用的有效部位包括水提物、醇提物、叶下珠生物碱、叶下珠多糖等。除显著的抗肝癌、肺癌、胃癌、乳腺癌等抗癌作用,叶下珠还具有抗病毒、抗氧化、抑菌、抗炎等药理作用。结合近5年的研究成果,文章对上述叶下珠有效部位和药理作用进行分析整理,同时提出今后关于叶下珠研究的建议,以此反映最新的叶下珠研究成果,为叶下珠临床应用提供理论依据,也为研究者下一步工作提供参考。

泡滇橄榄中一株酵母菌的分离鉴定及其耐受性研究

以泡滇橄榄为原料,对分离出的一株产气酵母菌进行鉴定并对其耐受性进行分析。从胀袋泡滇橄榄产品中分离获得一株产气酵母菌株,利用26S rDNA进行分子生物学鉴定,并开展耐受性及消毒剂抑制效果试验。结果表明:泡滇橄榄中产气菌株为葡萄汁有孢汉逊酵母(Hanseniaspora uvarum);该酵母菌具有耐酸性,在pH 2.5条件下依旧能够生长,而其耐热性能较差,75℃处理5 min便可将其杀死;pH值较低时,两种防腐剂(山梨酸钾、苯甲酸钠)对该酵母菌的抑制效果差异不明显,但pH值较高时,山梨酸钾的抑制效果较佳;4种消毒剂中对酵母菌抑制效果最好的为过氧乙酸,其次为次氯酸钠,而二氧化氯和二溴海因几乎无抑制作用。综上,泡滇橄中产气酵母为葡萄汁有孢汉逊酵母,具有较强耐酸性,过氧乙酸对其抑制效果较好。本研究为泡滇橄榄的工业化生产及滇橄榄系列产品开发提供一定的理论指导。

叶下珠水提液胃肠促动作用初探

目的对叶下珠水提液胃肠促动作用进行初步探究。方法应用在体小鼠胃排空和小肠向前推进性运动试验法,以及离体的家兔回肠肠管和豚鼠胃试验法,观察叶下珠水提液对动物胃、小肠活动情况的作用。结果相较于正常对照组,叶下珠水提液低、中、高剂量(2.5、5.0、10.0 g生药/kg)组能较明显地减少在体小鼠胃内残留率,也能较明显增加小肠向前推进运动百分比例,差异有统计学意义(P<0.05);在离体实验中,叶下珠水提液给药后明显增加离体家兔回肠肠管平滑肌收缩的幅度,相较于给药前差异有统计学意义(P<0.01),明显增加豚鼠胃底环形肌收缩的振幅和速度,相较于给药前差异也有统计学意义(P<0.01)。结论叶下珠水提液对胃肠具有明显促动作用。

叶下珠有效成分提纯工艺和质量控制研究进展

叶下珠主要成分包括黄酮类、酚酸类、木脂素类、生物碱类及多糖等,具有清热解毒、利水消肿、明目消积的功效,以及抗菌、抗氧化、抗病毒、抗血栓、提高脾细胞活性、长期使用毒副作用小的特点。临床上,叶下珠联合其他药物治疗乙型肝炎、脂肪肝效果理想。由于药效明确,开发利用前景良好,关于叶下珠各类成分提取利用和质量控制方面的研究及技术不断革新。为提供叶下珠药材开发利用的参考依据,本研究结合相关文献,综述了叶下珠部分有效成分的提纯工艺和近10年来质量控制方面的研究,发现(1)对于叶下珠中总酚类的提取,超声法适用于工业化生产,而柯里拉京的提取方面,膜过滤技术和柱色谱分离技术最有望用于工业生产;其他成分的提取工艺目前主要以传统方法为主,新方法处于实验探索阶段。(2)尽管有关叶下珠药理作用的研究较多,但是单个活性成分的构效关系研究较少,不利于进一步开发新药。(3)质量控制多集中在含量测定和指纹图谱方面,对重金属和33种农残的研究未见报道;市场上仍存在部分易混品常见的有苦味叶下珠、珠子草等,目前仍缺乏简单有效的鉴别手段。

叶下珠化学成分及其抗乙肝病毒活性的研究

对大戟科叶下珠属植物叶下珠的干燥全草进行了化学成分及其抗乙肝病毒活性的研究，共分得１１个化合物，其中２个新化合物为去氢诃子次酸甲酯、短叶苏木酚酸甲酯。通过抗病毒、抗乙肝表面抗原及保肝试验。

叶下珠提取物对酪氨酸酶的抑制和抗氧化作用

叶下珠为传统民间草药,具有平肝清热、利水解毒之功效.以叶下珠为研究对象,经乙醇提取,溶剂极性递增分离法制备获得叶下珠植物成分,测定了叶下珠植物对酪氨酸酶的抑制作用,以及运用1,1-二苯基-2-苦苯肼(DPPH)自由基法检测其清除自由基的能力.结果表明:叶下珠乙酸乙酯提取物和正丁醇提取物对酪氨酸酶二酚酶有较强抑制作用,IC50值分别达到0.26和0.49mg/mL,动力学研究表明:叶下珠乙酸乙酯提取物和正丁醇提取物对酪氨酸酶抑制作用均是可逆的,其抑制类型为混合型抑制作用;叶下珠乙酸乙酯提取物和正丁醇提取物对DPPH自由基具有一定的清除作用,在较低的浓度下有较高的清除效果,IC50值分别为0.012和0.008mg/mL,说明叶下珠植物具有开发成天然酪氨酸酶抑制剂和抗氧化剂的潜力.

不同产地叶下珠及其联合用药抗鸭乙型肝炎病毒的实验研究

本文应用重庆麻鸭乙型肝炎动物模型进行了不同产地中草药叶下珠及其联合中药苦参及抗菌药环丙沙星的抗鸭乙型肝炎病毒（ｄｕｃｋｈｅｐａｔｉｔｉｓＢＶｉｒｕｓ，ＤＨＢＶ）疗效初探，连续用药１个月后，结果表明：广西叶下珠、云南叶下珠能使鸭血清中鸭乙型肝炎病毒脱氧核糖核酸（ＤＨＢＶＤＮＡ）滴度下降（Ｐ＜０．０５），而重庆叶下珠无此作用（ｐ＞０．０５）；云南叶下珠停药后有血清ＤＨＢＶＤＮＡ回升现象；广西叶下珠与苦参合用，无明显协同作用，但与环丙沙星合用，似有增强抗病毒的作用（Ｐ＜０．０５）。

苦味叶下珠提取物对人肝癌细胞株PLC/PRF/5产生HBsAg的影响

实验表明较高浓度(4mg/ml)的叶下珠对人肝癌细胞株PLC/PRF/5细胞株细胞外HBsAg含量有明显的抑制作用,且主要影响HBsAg在该细胞内的合成。叶下珠与阿糖腺苷联合有一定的协同作用。

叶下珠抗肝细胞损伤作用机制

叶下珠能明显降低ＣＣｌ４引起的小鼠血清ＡＬＴ的升高，抑制肝脏ＭＤＡ的生成。叶下珠体外与大鼠肝细胞共同孵育，能抑制ＣＣｌ４所致肝细胞膜流动性降低，降低〔Ｃａ２＋〕ｉ浓度。结果提示，叶下珠保护肝脏损伤作用可能与其抗指质过氧化和膜保护作用有关。