A fragment with about 798 bp of cab gene was amplified from the first strand of Moso(Phyllostachys edulis)cDNA through RT-PCR method,named as cab-PhE4(cab gene 4 from Ph.edulis).The cab-PhE4(GenBank accession number:E...A fragment with about 798 bp of cab gene was amplified from the first strand of Moso(Phyllostachys edulis)cDNA through RT-PCR method,named as cab-PhE4(cab gene 4 from Ph.edulis).The cab-PhE4(GenBank accession number:EF405878)gene encodes 265 amino acid.The bioinformatics analysis indicated that the protein encoded by cab-PhE4 has a chlorophyll a/b-binding domain(64th-232nd position),a protein kinase C phosphorylation site(33rd-35th position),a N-myristoylation site(169th-174th position),and an involucrin repeat(207th-216th position).The amino acid sequence of cab-PhE4 showed high similarity with the cab genes of Zea mays,Triticum aestivum,Musa acuminata,Panax ginseng and Oryza sativa,more than 90%,respectively.展开更多
The light-harvesting chlorophyll a/b-protein complex plays an important role in photosynthesis of plants. A full-length cDNA of light-harvesting chlorophyll a/b （cab） gene was cloned from the first strand of Moso （...The light-harvesting chlorophyll a/b-protein complex plays an important role in photosynthesis of plants. A full-length cDNA of light-harvesting chlorophyll a/b （cab） gene was cloned from the first strand of Moso （Phyllostachys edulis） cDNA through RT-PCR and RACE methods, named as cabPhEIO （cab gene 10 from Ph. edulis）. The length of cab- PhEIO （GenBank accession number： EU118754） is 1 151 bp, which contains an open reading frame encoding 283 amino acids from 81st to 932nd position. The bioinformatics analysis indicated that the protein encoded by cab-PhElO had a chlorophll a/b binding domain （83rd -247th position）, two protein kinase C-phosphorylation sites, three Nmyristoylation sites and a yia A/B double helix domain.The amino acid sequence of cab-PhElO showed high similarity with the cab genes of Oryza sativa, Zea mays, Hordeum vulgare, and Vitis vinifera, more than 80%, respectively, which indicated that cab-PhElO gene belongs to lhcb5 gene family.展开更多
为了探究不同肥料对毛竹[Phyllostachys edulis(Carr.)H. de Lehaie]林出笋和新竹生长的影响,为毛竹林的精准施肥和科学经营提供理论依据。在江西省宜春市铜鼓县选择生长良好,具有代表性的毛竹纯林,在样地经垦复后,采用随机区组设计,设...为了探究不同肥料对毛竹[Phyllostachys edulis(Carr.)H. de Lehaie]林出笋和新竹生长的影响,为毛竹林的精准施肥和科学经营提供理论依据。在江西省宜春市铜鼓县选择生长良好,具有代表性的毛竹纯林,在样地经垦复后,采用随机区组设计,设置毛竹专用肥(ZYF)、矿渣肥(KZF)和不施肥(CK)处理,每个处理3次重复,共9块20 m×20 m的样地,研究不同肥料处理对毛竹出笋和新竹生长的影响。结果表明:两种肥料均在一定程度上提高了出笋数、退笋数和成竹数。毛竹专用肥处理出笋数和退笋数最高,分别较对照高出19.48%和51.17%,矿渣肥处理较对照处理分别高出16.05%和16.96%;而矿渣肥处理的成竹数最高,其次为毛竹专用肥处理,二者分别比对照高175、108株/hm^2。不同施肥处理间,竹笋地径总体呈毛竹专用肥处理最高,其次分别为矿渣肥和对照处理,而笋高在不同处理间的波动较小。2种肥料处理显著提高了新竹胸径和大径材毛竹的比例,并以毛竹专用肥处理效果更为明显。毛竹专用肥处理新竹枝下高显著高于对照处理,而矿渣肥与对照处理间无显著差异。结果表明毛竹专用肥更有利于作为以生产竹笋、大径材竹为目的的毛竹林培肥措施,矿渣肥对促进毛竹林优质、丰产的短期肥效低于毛竹专用肥,可能与其为缓释肥有关。展开更多
基金This project is supported by"948"introduction project(2004-4-60,2005-4-38)
文摘A fragment with about 798 bp of cab gene was amplified from the first strand of Moso(Phyllostachys edulis)cDNA through RT-PCR method,named as cab-PhE4(cab gene 4 from Ph.edulis).The cab-PhE4(GenBank accession number:EF405878)gene encodes 265 amino acid.The bioinformatics analysis indicated that the protein encoded by cab-PhE4 has a chlorophyll a/b-binding domain(64th-232nd position),a protein kinase C phosphorylation site(33rd-35th position),a N-myristoylation site(169th-174th position),and an involucrin repeat(207th-216th position).The amino acid sequence of cab-PhE4 showed high similarity with the cab genes of Zea mays,Triticum aestivum,Musa acuminata,Panax ginseng and Oryza sativa,more than 90%,respectively.
基金This project is supported by "948" Introduction Project (2004-4-60, 2005-4-38)
文摘The light-harvesting chlorophyll a/b-protein complex plays an important role in photosynthesis of plants. A full-length cDNA of light-harvesting chlorophyll a/b （cab） gene was cloned from the first strand of Moso （Phyllostachys edulis） cDNA through RT-PCR and RACE methods, named as cabPhEIO （cab gene 10 from Ph. edulis）. The length of cab- PhEIO （GenBank accession number： EU118754） is 1 151 bp, which contains an open reading frame encoding 283 amino acids from 81st to 932nd position. The bioinformatics analysis indicated that the protein encoded by cab-PhElO had a chlorophll a/b binding domain （83rd -247th position）, two protein kinase C-phosphorylation sites, three Nmyristoylation sites and a yia A/B double helix domain.The amino acid sequence of cab-PhElO showed high similarity with the cab genes of Oryza sativa, Zea mays, Hordeum vulgare, and Vitis vinifera, more than 80%, respectively, which indicated that cab-PhElO gene belongs to lhcb5 gene family.
