[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to constru...[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to construct the full-length cDNA library by using Oligo-capping method. [Result] The constructed library has a total capacity of 6.5×10^6 recombinant clones, and a low proportion of clones without inserted frag- ments; the size of inserted fragments ranges between 0.3-5.0 kb, with strict classifi- cation and ideal consistency. Furthermore, the proportion of clones harboring long in- serted fragments （1.0-5.0 kb） is as high as 30%, achieving the standard for high- quality full-length cDNA library. [Conclusion] The full-length cDNA library of germinat- ing seeds of P. heterocycla was successfully constructed, which laid important foun- dation for the functional genomics research of bamboo plants.展开更多
Biomineralization of Si by plants into phytolith formation and precipitation of Si into clays during weathering are two important processes of silicon’s biogeochemical cycle. As a silicon-accumulating plant, the wide...Biomineralization of Si by plants into phytolith formation and precipitation of Si into clays during weathering are two important processes of silicon’s biogeochemical cycle. As a silicon-accumulating plant, the widely distributed and woody Phyl-lostachys heterocycla var. pubescens (moso bamboo) contributes to storing silicon by biomineralization and, thus, prevents eu-trophication of nearby waterbodies through silicon’s erosion of soil particles. A study on the organic pool and biological cycle of silicon (Si) of the moso bamboo community was conducted in Wuyishan Biosphere Reserve, China. The results showed that: (1) the standing crop of the moso bamboo community was 13355.4 g/m2, of which 53.61%, 45.82% and 0.56% are represented by the aboveground and belowground parts of moso bamboos, and the under-story plants, respectively; (2) the annual net primary production of the community was 2887.1 g/(m2·a), among which the aboveground part, belowground part, litterfalls, and other fractions, accounted for 55.86%, 35.30%, 4.50% and 4.34%, respec-tively; (3) silicon concentration in stem, branch, leaf, base of stem, root, whip of bamboos, and other plants was 0.15%, 0.79%, 3.10%, 4.40%, 7.32%, 1.52% and 1.01%, respectively; (4) the total Si accumulated in the standing crop of moso bamboo com-munity was 448.91 g/m2, with 99.83% of Si of the total community stored in moso bamboo populations; (5) within moso bamboo community, the annual uptake, retention, and return of Si were 95.75, 68.43, 27.32 g/(m2·a), respectively; (6) the turnover time of Si, which is the time an average atom of Si remains in the soil before it is recycled into the trees or shrubs, was 16.4 years; (7) the enrichment ratio of Si in the moso bamboo community, which is the ratio of the mean concentration of nutrients in the net primary production to the mean concentration of nutrients in the biomass of a community, was 0.64; and lastly, (8) moso bamboo plants stored about 1.26×1010 kg of silicon in the organic pool made up by the moso bamboo forests in the subtropical area of China.展开更多
With the increase of the utilization of bamboo, we must find out more in bamboo properties. According to the national standard GB/T15780 -1995, this paper analyzed the air-dried density and mechanical properties of Mo...With the increase of the utilization of bamboo, we must find out more in bamboo properties. According to the national standard GB/T15780 -1995, this paper analyzed the air-dried density and mechanical properties of Moso bamboo in different ages and stalk parts. The results showed that both the age and the stalk part have remarkable effects on air-dried density and mechanical properties. The effect of age is much more prominent than that of stalk part. The performance shows: upper > middle > down, which increases with the age, and reaches to the peak values at the age of 4 and show a downward trend at the age of 6. According to the tests, the optimum age of bamboo for cutting is about at 4. Moreover, there is a positive correlation between air-dried density and mechanical properties. The correlation coefficients between air-dried density and crushing strength parallel to the grain, bending strength, modulus of elasticity and tensile strength parallel to the grain are 0.917 3, 0.783 9, 0.745 8 and 0.507 4, respectively. In addition, there is a good relativity among mechanical properties, δt: δc: MOR: MOE = 2.79: 1.00: 2.40: 0.15.展开更多
In order to further research bamboo cultivation and expand its application field, air-dried density and mechanical properties of Moso bamboo from the 2 plantations under the same site condition were measured and analy...In order to further research bamboo cultivation and expand its application field, air-dried density and mechanical properties of Moso bamboo from the 2 plantations under the same site condition were measured and analyzed by using the national standard The Testing Methods for Physical and Mechanical Properties of Bamboo (GB / T 15780-1995). And the single linear regression analysis was carried out. The results showed that air-dried density and mechanical properties of the 2 kinds of Moso bamboo increase with the age increasing, and at the same age, those of Moso bamboo from the seedling plantation are much higher than those from the common plantation afforested by transplanting, in other words, Moso bamboo from the conventional plantation reaches a stabilization the at age of 4, while that from the seedling plantation reaches a stabilization at the age of 6 or more. There is a positive correlation between air-dried density and mechanical properties of the 2 kinds of bamboo.展开更多
Bamboo as an important biological resource has become an alternative to wood gradually,but the variation in mechanical properties leads to difficulty in its use to some extent.So deep understanding of the nature in ba...Bamboo as an important biological resource has become an alternative to wood gradually,but the variation in mechanical properties leads to difficulty in its use to some extent.So deep understanding of the nature in bamboo is needed to satisfy the utilization effectively.As we have known,the density is one of mechanical properties of bamboo.Therefore,this paper studied the variation in bamboo density with the weighing method.The results showed that the density decreases gradually from the outer to the inn...展开更多
基金Supported by Specialized Fund for the Basic Research Operating Expenses Program of International Centre for Bamboo and Rattan(163201300812618-7)Special Fund for Research and Development of Forestry Nonprofit Industry(200704001)~~
文摘[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to construct the full-length cDNA library by using Oligo-capping method. [Result] The constructed library has a total capacity of 6.5×10^6 recombinant clones, and a low proportion of clones without inserted frag- ments; the size of inserted fragments ranges between 0.3-5.0 kb, with strict classifi- cation and ideal consistency. Furthermore, the proportion of clones harboring long in- serted fragments （1.0-5.0 kb） is as high as 30%, achieving the standard for high- quality full-length cDNA library. [Conclusion] The full-length cDNA library of germinat- ing seeds of P. heterocycla was successfully constructed, which laid important foun- dation for the functional genomics research of bamboo plants.
基金Project (No. 30370275) supported by the National Natural Science Foundation of China
文摘Biomineralization of Si by plants into phytolith formation and precipitation of Si into clays during weathering are two important processes of silicon’s biogeochemical cycle. As a silicon-accumulating plant, the widely distributed and woody Phyl-lostachys heterocycla var. pubescens (moso bamboo) contributes to storing silicon by biomineralization and, thus, prevents eu-trophication of nearby waterbodies through silicon’s erosion of soil particles. A study on the organic pool and biological cycle of silicon (Si) of the moso bamboo community was conducted in Wuyishan Biosphere Reserve, China. The results showed that: (1) the standing crop of the moso bamboo community was 13355.4 g/m2, of which 53.61%, 45.82% and 0.56% are represented by the aboveground and belowground parts of moso bamboos, and the under-story plants, respectively; (2) the annual net primary production of the community was 2887.1 g/(m2·a), among which the aboveground part, belowground part, litterfalls, and other fractions, accounted for 55.86%, 35.30%, 4.50% and 4.34%, respec-tively; (3) silicon concentration in stem, branch, leaf, base of stem, root, whip of bamboos, and other plants was 0.15%, 0.79%, 3.10%, 4.40%, 7.32%, 1.52% and 1.01%, respectively; (4) the total Si accumulated in the standing crop of moso bamboo com-munity was 448.91 g/m2, with 99.83% of Si of the total community stored in moso bamboo populations; (5) within moso bamboo community, the annual uptake, retention, and return of Si were 95.75, 68.43, 27.32 g/(m2·a), respectively; (6) the turnover time of Si, which is the time an average atom of Si remains in the soil before it is recycled into the trees or shrubs, was 16.4 years; (7) the enrichment ratio of Si in the moso bamboo community, which is the ratio of the mean concentration of nutrients in the net primary production to the mean concentration of nutrients in the biomass of a community, was 0.64; and lastly, (8) moso bamboo plants stored about 1.26×1010 kg of silicon in the organic pool made up by the moso bamboo forests in the subtropical area of China.
基金supported by The 11th Five-Year Plan: The Project of National Science and Technology Supporting (NO.2006BAD19B0402)
文摘With the increase of the utilization of bamboo, we must find out more in bamboo properties. According to the national standard GB/T15780 -1995, this paper analyzed the air-dried density and mechanical properties of Moso bamboo in different ages and stalk parts. The results showed that both the age and the stalk part have remarkable effects on air-dried density and mechanical properties. The effect of age is much more prominent than that of stalk part. The performance shows: upper > middle > down, which increases with the age, and reaches to the peak values at the age of 4 and show a downward trend at the age of 6. According to the tests, the optimum age of bamboo for cutting is about at 4. Moreover, there is a positive correlation between air-dried density and mechanical properties. The correlation coefficients between air-dried density and crushing strength parallel to the grain, bending strength, modulus of elasticity and tensile strength parallel to the grain are 0.917 3, 0.783 9, 0.745 8 and 0.507 4, respectively. In addition, there is a good relativity among mechanical properties, δt: δc: MOR: MOE = 2.79: 1.00: 2.40: 0.15.
文摘In order to further research bamboo cultivation and expand its application field, air-dried density and mechanical properties of Moso bamboo from the 2 plantations under the same site condition were measured and analyzed by using the national standard The Testing Methods for Physical and Mechanical Properties of Bamboo (GB / T 15780-1995). And the single linear regression analysis was carried out. The results showed that air-dried density and mechanical properties of the 2 kinds of Moso bamboo increase with the age increasing, and at the same age, those of Moso bamboo from the seedling plantation are much higher than those from the common plantation afforested by transplanting, in other words, Moso bamboo from the conventional plantation reaches a stabilization the at age of 4, while that from the seedling plantation reaches a stabilization at the age of 6 or more. There is a positive correlation between air-dried density and mechanical properties of the 2 kinds of bamboo.
基金supported by The 11th Five-year Plan "The Project of National Science and Technology Supporting(2006BAD19B0402)"
文摘Bamboo as an important biological resource has become an alternative to wood gradually,but the variation in mechanical properties leads to difficulty in its use to some extent.So deep understanding of the nature in bamboo is needed to satisfy the utilization effectively.As we have known,the density is one of mechanical properties of bamboo.Therefore,this paper studied the variation in bamboo density with the weighing method.The results showed that the density decreases gradually from the outer to the inn...
