Main Agronomic Characters and Grain Quality of Rice Blast Resistance Gene Pi-d2 Transgenic Rice

[Objective] The aim of this study was to provide metabolic evidence for the analysis of the ecological and safety assessment of Pi-d2-transgenic rice.[Method] The main agronomic characters of Pi-d2-transgenic rice were observed in field experiment and the grain chemical characters and amino acid content were measured.[Results] Introduction of foreign gene Pi-d2 resulted in stably hereditable variation in agronomic characteristics in the descents.Most of the transgenic lines grew normally and orderly.Compared with the control（wild type plants）,about half of transgenic plants showed an increased or reduced plant height.There was no observable difference between transgenic plants and controls in tiller number,length of panicle,panicles per plant,seed-setting rate and 1 000-grain weight.Total amino acid content in transgenic rice was reduced,while the starch content,GC and GT were not altered in comparison with the control.[Conclusion] Introduction of foreign gene Pi-d2 has remarkable influence on plant height,while little on grain chemical characters.

转稻瘟病抗性基因Pi-d2水稻的主要农艺性状和米质研究

[目的]对农杆菌介导转化获得的稻瘟病抗性基因Pi-d2的水稻植株稳定后代的主要农艺性状和米质进行了分析,以期为转基因水稻的生态学和安全性评价提供依据。[方法]在田间调查了转Pi-d2基因水稻植株的主要农艺性状,并对转基因水稻稻米理化特性和氨基酸含量进行测定。[结果]外源基因Pi-d2的导入可引起受体植株后代农艺性状可遗传的变异。大部分转基因植株生长正常,整齐一致,与对照相比,一半左右植株株高表现显著差异;分蘖数、穗长、有效穗、结实率和千粒重与对照相比无明显差异,少部分表现增加或减少的趋势。稻米的氨基酸测定结果表明转抗病基因水稻改变了原亲本的总氨基酸含量,呈下降趋势,但淀粉含量、胶稠度和糊化温度并未改变。[结论]将Pi-d2基因转入水稻中对植株株高影响明显,但对稻米的理化特性影响不大。

水稻抗稻瘟病基因Pi-d2基因标签的建立与应用

建立抗稻瘟病基因的分子标记对培育抗稻瘟病品种有重要意义。研究利用71个广西地方菌株检测地谷对稻瘟病菌的抗性,并利用地谷中Pi-d2基因与广恢998等位基因在基因组序列上一个碱基的差异,设计出以抗病基因本身序列为引物的基因标签M-Pid2,通过分析地谷与广恢998的F2群体的基因型与对稻瘟病抗感分离的吻合度来验证该标签的准确性,用M-Pid2扩增62份水稻种质验证该标签的特异性。结果表明,地谷能抗71个地方稻瘟病菌株中的59个菌株,抗性频率达83.1%。用M-Pid2扩增地谷与广恢998的F2群体的基因型与其对稻瘟病的抗感分离完全吻合;用M-Pid2扩增62份水稻种质,仅在地谷中扩增出629bp特异条带。从而确认抗稻瘟病基因Pi-d2为地谷的主效抗稻瘟病基因,且在广西有育种利用价值;标记M-Pid2能准确用于抗稻瘟病基因Pi-d2的辅助选择。