Picea crassifolia Kom, a perennial arbor spe- cies is recognized as one of the most adaptable plants found to date in Qilian Mountains. To explore the cutting reproduction technology of P. crassifolia and reveal its r...Picea crassifolia Kom, a perennial arbor spe- cies is recognized as one of the most adaptable plants found to date in Qilian Mountains. To explore the cutting reproduction technology of P. crassifolia and reveal its rooting mechanism, cuttings of P. crassifolia with different cutting types （softwood, hardwood and root）, positions （top, upper, middle and bottom） and ages （7, 10, 15, 20, 25 year-old） were cultivated in a field experiment. One- year old softwood and hardwood cuttings were collected from 7-, 10-, 15-, 20-, and 25 year-old healthy ortets to analyze the changes from endogenous hormones and organic nutrients. Results indicate that the softwood cut- tings （0.5-1.0 cm in diameter） from upper branches of 15 year-old ortets shows better growth performance by improving rooting indexes, including a significant increasein rooting rate and a decrease in basal rot rate. Concomi- tantly, increasing rooting quantity and root length also increased. It is noteworthy that the high rooting rate of P. crassifolia cuttings due to its ability to accumulate high concentrations of indole-3-acetic acid （IAA） and total carbon （TC） rather than abscisic acid （ABA） and total nitrogen （TN）. The rooting rate was mainly regulated by the IAA/ABA and TC/TN ratio. In summary, our results suggest that the softwood cuttings （0.5-1.0 cm in diameter） from upper branches of 15 year-old P. crassifolia can be considered as an effective strategy to improve cutting rooting rate, and the IAA/ABA and TC/TN ratio was one of the main factors limiting the cutting rooting rate of P. crassifolia.展开更多
为了解青海云杉插穗生根的外在因素和内在生理生化基础,采用网袋容器和自动间歇喷雾扦插繁殖技术,系统研究了青海云杉插穗母株年龄、部位、类型及激素处理等对插穗生根的影响,并采用高效液相色谱法测定了不同插穗类型内源激素吲哚乙酸(I...为了解青海云杉插穗生根的外在因素和内在生理生化基础,采用网袋容器和自动间歇喷雾扦插繁殖技术,系统研究了青海云杉插穗母株年龄、部位、类型及激素处理等对插穗生根的影响,并采用高效液相色谱法测定了不同插穗类型内源激素吲哚乙酸(IAA)、脱落酸(ABA)、赤霉素(GA3)、玉米素(Z)、玉米素核苷(ZR)、异戊烯基腺嘌呤(IPA)、酚类化合物和糖类的含量。结果表明,以7年生母株枝条作穗条扦插繁殖效果最佳;一级侧枝的生根情况优于二级侧枝和顶枝穗条,树冠下部穗条生根优于上部枝条;激素处理能显著提高硬枝插穗的生根率,其中以IBA 200 m g/kg处理3 h的生根效果最好;邻苯二酸和儿茶酸2种酚类化合物可能对生根有抑制作用;枝条和针叶中三糖含量随母株年龄增加而增大,而葡萄糖含量却呈下降趋势;高含量的Z和ZR均有利于插条生根,IAA对生根无促进作用,IPA对插条生根可能具有一定的抑制作用,单一的某种激素对青海云杉插穗生根无明显影响。展开更多
基金supported by the National Natural Science Foundation of China(Grant No.31360086)the grant from the major state basic research development program of china(973 program,No.2013CB429903)
文摘Picea crassifolia Kom, a perennial arbor spe- cies is recognized as one of the most adaptable plants found to date in Qilian Mountains. To explore the cutting reproduction technology of P. crassifolia and reveal its rooting mechanism, cuttings of P. crassifolia with different cutting types （softwood, hardwood and root）, positions （top, upper, middle and bottom） and ages （7, 10, 15, 20, 25 year-old） were cultivated in a field experiment. One- year old softwood and hardwood cuttings were collected from 7-, 10-, 15-, 20-, and 25 year-old healthy ortets to analyze the changes from endogenous hormones and organic nutrients. Results indicate that the softwood cut- tings （0.5-1.0 cm in diameter） from upper branches of 15 year-old ortets shows better growth performance by improving rooting indexes, including a significant increasein rooting rate and a decrease in basal rot rate. Concomi- tantly, increasing rooting quantity and root length also increased. It is noteworthy that the high rooting rate of P. crassifolia cuttings due to its ability to accumulate high concentrations of indole-3-acetic acid （IAA） and total carbon （TC） rather than abscisic acid （ABA） and total nitrogen （TN）. The rooting rate was mainly regulated by the IAA/ABA and TC/TN ratio. In summary, our results suggest that the softwood cuttings （0.5-1.0 cm in diameter） from upper branches of 15 year-old P. crassifolia can be considered as an effective strategy to improve cutting rooting rate, and the IAA/ABA and TC/TN ratio was one of the main factors limiting the cutting rooting rate of P. crassifolia.
文摘为了解青海云杉插穗生根的外在因素和内在生理生化基础,采用网袋容器和自动间歇喷雾扦插繁殖技术,系统研究了青海云杉插穗母株年龄、部位、类型及激素处理等对插穗生根的影响,并采用高效液相色谱法测定了不同插穗类型内源激素吲哚乙酸(IAA)、脱落酸(ABA)、赤霉素(GA3)、玉米素(Z)、玉米素核苷(ZR)、异戊烯基腺嘌呤(IPA)、酚类化合物和糖类的含量。结果表明,以7年生母株枝条作穗条扦插繁殖效果最佳;一级侧枝的生根情况优于二级侧枝和顶枝穗条,树冠下部穗条生根优于上部枝条;激素处理能显著提高硬枝插穗的生根率,其中以IBA 200 m g/kg处理3 h的生根效果最好;邻苯二酸和儿茶酸2种酚类化合物可能对生根有抑制作用;枝条和针叶中三糖含量随母株年龄增加而增大,而葡萄糖含量却呈下降趋势;高含量的Z和ZR均有利于插条生根,IAA对生根无促进作用,IPA对插条生根可能具有一定的抑制作用,单一的某种激素对青海云杉插穗生根无明显影响。
