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Predicting Reference Evaporation for the Ethiopian Highlands
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作者 Anwar A. Adem Dessalew W. Aynalem +1 位作者 Seifu A. Tilahun Tammo S. Steenhuis 《Journal of Water Resource and Protection》 2017年第11期1244-1269,共26页
Water is likely the most limiting factor in increasing agricultural production in large parts of Africa. Reference evaporation (ET0) is a key hydrological parameter to use efficiently the scarce supply. Several method... Water is likely the most limiting factor in increasing agricultural production in large parts of Africa. Reference evaporation (ET0) is a key hydrological parameter to use efficiently the scarce supply. Several methods are available for predicting reference evaporation, but the accuracy of any of the methods has not been established for the Ethiopian highlands. The objective of this study is, therefore, to select the best methods for calculating the reference evaporation ET0. For the section, meteorological data of the Bahir Dar station were used, because all data needed for this study including the Class A pan Evaporation were recorded on a daily basis. Pan evaporation was considered as the best estimator of the reference evaporation. The results showed that the FAO-Penman Monteith (using solar radiation, wind speed, temperature and relative humidity) and Enku method (using only maximum daily temperatures) have acceptable daily ET0 ranges and predicted to Class A pan evaporation with correlation coefficients greater than 90% in a monthly basis. Next best was the Thornthwaite’s method with correlation coefficient of 89% with pan evaporation. Piche methods performed relatively well with correlation coefficient of greater than 70%. Blaney-Criddle, Priestley & Taylor, and Hargreaves performed the poorest in predicting pan evaporation. These methods should be recalibrated for local condition and therefore not recommended for use in the Ethiopian highlands. In summary, the FAO-Penman Monteith is recommended for locations where the input data are available;otherwise, the Enku method using maximum daily temperature is best for estimating the reference evaporation. 展开更多
关键词 PAN piche AFRICA Potential EVAPORATION FAO-56
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Modeling and Free Vibration Behavior of Rotating Composite Thin-walled Closed-section Beams with SMA Fibers 被引量:4
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作者 REN Yongsheng YANG Shulian DU Xianghong 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2012年第5期1029-1043,共15页
Smart structure with active materials embedded in a rotating composite thin-walled beam is a class of typical structure which is using in study of vibration control of helicopter blades and wind turbine blades. The dy... Smart structure with active materials embedded in a rotating composite thin-walled beam is a class of typical structure which is using in study of vibration control of helicopter blades and wind turbine blades. The dynamic behavior investigation of these structures has significance in theory and practice. However, so far dynamic study on the above-mentioned structures is limited only the rotating composite beams with piezoelectric actuation. The free vibration of the rotating composite thin-walled beams with shape memory alloy(SMA) fiber actuation is studied. SMA fiber actuators are embedded into the walls of the composite beam. The equations of motion are derived based on Hamilton's principle and the asymptotically correct constitutive relation of single-cell cross-section accounting for SMA fiber actuation. The partial differential equations of motion are reduced to the ordinary differential equations of motion by using the Galerkin's method. The formulation for free vibration analysis includes anisotropy, pitch and precone angle, centrifugal force and SMA actuation effect. Numerical results of natural frequency are obtained for two configuration composite beams. It is shown that natural frequencies of the composite thin-walled beam decrease as SMA fiber volume and initial strain increase and the decrease in natural frequency becomes more significant as SMA fiber volume increases. The actuation performance of SMA fibers is found to be closely related to the rotational speeds and ply-angle. In addition, the effect of the pitch angle appears to be more significant for the lower-bending mode ones. Finally, in all cases, the precone angle appears to have marginal effect on free vibration frequencies. The developed model can be capable of describing natural vibration behaviors of rotating composite thin-walled beam with active SMA fiber actuation. The present work extends the previous analysis done for modeling passive rotating composite thin-walled beam. 展开更多
关键词 free vibration thin-walled composite beams shape memory alloy rotating beams pich angle precone angle
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可诱导型敲低PICH的MDA-MB-231细胞系的构建及鉴定
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作者 李万金 黄燕 +3 位作者 吕璐冶 韩秋影 周涛 陈亮 《生物技术通讯》 CAS 2019年第2期153-157,共5页
目的:构建稳定表达可诱导型polo样激酶1相关检查点解旋酶(PICH)短发夹RNA(shRNA)的三阴乳腺癌细胞系MDA-MB-231,并通过检测加入诱导剂后细胞的增殖评价敲低效果。方法:设计并合成2条特异靶向PICH的shRNA序列,用分子克隆技术构建pLKO.1-t... 目的:构建稳定表达可诱导型polo样激酶1相关检查点解旋酶(PICH)短发夹RNA(shRNA)的三阴乳腺癌细胞系MDA-MB-231,并通过检测加入诱导剂后细胞的增殖评价敲低效果。方法:设计并合成2条特异靶向PICH的shRNA序列,用分子克隆技术构建pLKO.1-tet-on-shPICH-Puromycin质粒,经菌落PCR及基因测序验证;慢病毒包装并感染细胞,通过抗性筛选获得嘌呤霉素抗性的细胞;经强力霉素(DOX)诱导表达后,Western印迹检测PICH的敲低效果,并通过MTT法对细胞增殖进行检测。结果:菌落PCR凝胶电泳及DNA测序结果显示pLKO.1-tet-on-shPICHPuromycin质粒构建成功;Western印迹结果表明,在DOX诱导下,MDA-MB-231细胞中的PICH蛋白质表达下降,表明可诱导型PICH稳定敲低细胞系构建成功;进一步实验结果显示,MDA-MB-231细胞的增殖随PICH的表达下调而受到显著抑制。结论:构建了可诱导稳定敲低PICH的三阴乳腺癌细胞系MDA-MB-231,为进一步研究PICH在三阴乳腺癌中的作用以及具体的分子机制奠定了实验基础。 展开更多
关键词 polo样激酶1相关检查点解旋酶(PICH) 三阴乳腺癌 可诱导稳定敲低 细胞增殖
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