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A regression approach for seismic first-break picking
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作者 Huan Yuan San-Yi Yuan +2 位作者 Jie Wu Wen-Jing Sang Yu-He Zhao 《Petroleum Science》 SCIE EI CAS CSCD 2024年第3期1584-1596,共13页
The picking efficiency of seismic first breaks(FBs)has been greatly accelerated by deep learning(DL)technology.However,the picking accuracy and efficiency of DL methods still face huge challenges in low signal-to-nois... The picking efficiency of seismic first breaks(FBs)has been greatly accelerated by deep learning(DL)technology.However,the picking accuracy and efficiency of DL methods still face huge challenges in low signal-to-noise ratio(SNR)situations.To address this issue,we propose a regression approach to pick FBs based on bidirectional long short-term memory(Bi LSTM)neural network by learning the implicit Eikonal equation of 3D inhomogeneous media with rugged topography in the target region.We employ a regressive model that represents the relationships among the elevation of shots,offset and the elevation of receivers with their seismic traveltime to predict the unknown FBs,from common-shot gathers with sparsely distributed traces.Different from image segmentation methods which automatically extract image features and classify FBs from seismic data,the proposed method can learn the inner relationship between field geometry and FBs.In addition,the predicted results by the regressive model are continuous values of FBs rather than the discrete ones of the binary distribution.The picking results of synthetic data shows that the proposed method has low dependence on label data,and can obtain reliable and similar predicted results using two types of label data with large differences.The picking results of9380 shots for 3D seismic data generated by vibroseis indicate that the proposed method can still accurately predict FBs in low SNR data.The subsequent stacked profiles further illustrate the reliability and effectiveness of the proposed method.The results of model data and field seismic data demonstrate that the proposed regression method is a robust first-break picker with high potential for field application. 展开更多
关键词 First-break picking Low signal-to-noiseratio Regression BiLSTM TRAVELTIME Geometry Noisy seismic data
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Automatic velocity picking based on optimal key points tracking algorithm
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作者 Yong-Hao Wang Wen-Kai Lu +3 位作者 Song-Bai Jin Yang Li Yu-Xuan Li Xiao-Feng Gu 《Petroleum Science》 SCIE EI CAS CSCD 2024年第2期903-917,共15页
Picking velocities from semblances manually is laborious and necessitates experience. Although various methods for automatic velocity picking have been developed, there remains a challenge in efficiently incorporating... Picking velocities from semblances manually is laborious and necessitates experience. Although various methods for automatic velocity picking have been developed, there remains a challenge in efficiently incorporating information from nearby gathers to ensure picked velocity aligns with seismic horizons while also improving picking accuracy. The conventional method of velocity picking from a semblance volume is computationally demanding, highlighting a need for a more efficient strategy. In this study, we introduce a novel method for automatic velocity picking based on multi-object tracking. This dynamic tracking process across different semblance panels can integrate information from nearby gathers effectively while maintaining computational efficiency. First, we employ accelerated density clustering on the velocity spectrum to discern cluster centers without the requirement for prior knowledge regarding the number of clusters. These cluster centers embody the maximum likelihood velocities of the main subsurface structures. Second, our proposed method tracks key points within the semblance volume. Kalman filter is adopted to adjust the tracking process, followed by interpolation on these tracked points to construct the final velocity model. Our synthetic data example demonstrates that our proposed algorithm can effectively rectify the picking errors of the clustering algorithm. We further compare the performances of the clustering method(CM), the proposed tracking method(TM), and the variational method(VM) on a field dataset from the Gulf of Mexico. The results attest that our method offers superior accuracy than CM, achieves comparable accuracy with VM, and benefits from a reduced computational cost. 展开更多
关键词 Velocity picking Multi-object tracking Density clustering Kalman filter
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First-Arrival Picking Method for Active Source Data with Ocean Bottom Seismometers Based on Spatial Waveform Variation Characteristics
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作者 LIU Hongwei XING Lei +3 位作者 ZHU Henghua ZHANG Jin ZHANG Jing LIU Huaishan 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第4期970-980,共11页
The precision and reliability of first-arrival picking are crucial for determining the accuracy of geological structure inversion using active source ocean bottom seismometer(OBS)refraction data.Traditional methods fo... The precision and reliability of first-arrival picking are crucial for determining the accuracy of geological structure inversion using active source ocean bottom seismometer(OBS)refraction data.Traditional methods for first-arrival picking based on sample points are characterized by theoretical errors,especially in low-sampling-frequency OBS data because the travel time of seismic waves is not an integer multiple of the sampling interval.In this paper,a first-arrival picking method that utilizes the spatial waveform variation characteristics of active source OBS data is presented.First,the distribution law of theoretical error is examined;adjacent traces exhibit variation characteristics in their waveforms.Second,a label cross-correlation superposition method for extracting highfrequency signals is presented to enhance the first-arrival picking precision.Results from synthetic and field data verify that the proposed approach is robust,successfully overcomes the limitations of low sampling frequency,and achieves precise outcomes that are comparable with those of high-sampling-frequency data. 展开更多
关键词 first-arrival picking spatial waveform variation label cross-correlation superposition method
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CREDIT-X1local:A reference dataset for machine learning seismology from ChinArray in Southwest China
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作者 Lu Li Weitao Wang +1 位作者 Ziye Yu Yini Chen 《Earthquake Science》 2024年第2期139-157,共19页
High-quality datasets are critical for the development of advanced machine-learning algorithms in seismology.Here,we present an earthquake dataset based on the ChinArray Phase I records(X1).ChinArray Phase I was deplo... High-quality datasets are critical for the development of advanced machine-learning algorithms in seismology.Here,we present an earthquake dataset based on the ChinArray Phase I records(X1).ChinArray Phase I was deployed in the southern north-south seismic zone(20°N-32°N,95°E-110°E)in 2011-2013 using 355 portable broadband seismic stations.CREDIT-X1local,the first release of the ChinArray Reference Earthquake Dataset for Innovative Techniques(CREDIT),includes comprehensive information for the 105,455 local events that occurred in the southern north-south seismic zone during array observation,incorporating them into a single HDF5 file.Original 100-Hz sampled three-component waveforms are organized by event for stations within epicenter distances of 1,000 km,and records of≥200 s are included for each waveform.Two types of phase labels are provided.The first includes manually picked labels for 5,999 events with magnitudes≥2.0,providing 66,507 Pg,42,310 Sg,12,823 Pn,and 546 Sn phases.The second contains automatically labeled phases for 105,442 events with magnitudes of−1.6 to 7.6.These phases were picked using a recurrent neural network phase picker and screened using the corresponding travel time curves,resulting in 1,179,808 Pg,884,281 Sg,176,089 Pn,and 22,986 Sn phases.Additionally,first-motion polarities are included for 31,273 Pg phases.The event and station locations are provided,so that deep learning networks for both conventional phase picking and phase association can be trained and validated.The CREDIT-X1local dataset is the first million-scale dataset constructed from a dense seismic array,which is designed to support various multi-station deep-learning methods,high-precision focal mechanism inversion,and seismic tomography studies.Additionally,owing to the high seismicity in the southern north-south seismic zone in China,this dataset has great potential for future scientific discoveries. 展开更多
关键词 earthquake dataset machine learning Pg/Sg/Pn/Sn phase picking P-wave first-motion polarity
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Manual Order Picking Route Optimization in Distribution Warehouse of Chain Furniture Retail Enterprise
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作者 Yongzhen Zeng Junkun Wang +1 位作者 Ching-Kuei Kao King-Zoo Tang 《American Journal of Operations Research》 2024年第3期105-136,共32页
Due to the effects of the COVID-19 pandemic and the rise of online shopping, the offline sales of IKEA Fuzhou have been declining since 2020. Because the cost of distribution warehouse is a major expense for offline c... Due to the effects of the COVID-19 pandemic and the rise of online shopping, the offline sales of IKEA Fuzhou have been declining since 2020. Because the cost of distribution warehouse is a major expense for offline chain furniture retailers, and the picking process is a key activity in distribution warehouse operations. To reduce the cost of distribution warehouse and alleviate the survival pressure of the offline chain furniture retailers, this paper focuses on optimizing the picking route of the IKEA Fuzhou distribution warehouse. It starts by creating a two-dimensional coordinate system for the storage location of the distribution warehouse using the traditional S-type picking strategy to calculate the distance and time of the sorting route. Then, the problem of optimizing the picking route is then transformed into the travelling salesman problem (TSP), and picking route optimization model is developed using a genetic algorithm to analyze the sorting efficiency and picking route optimization. Results show that the order-picking route using the genetic algorithm strategy is significantly better than the traditional S-type picking strategy, which can improve overall sorting efficiency and operations, reduce costs, and increase efficiency. Thus, this establishes an implementation process for the order-picking path based on genetic algorithm optimization to improve overall sorting efficiency and operations, reduce costs, increase efficiency, and alleviate the survival pressure of pandemic-affected enterprises. 展开更多
关键词 S-Shaped Picking Strategy Picking Route Traveling Salesman Problem Genetic Algorithm
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Rich Pickings for Children
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作者 AN ZI LIU XIAOHONG 《Women of China》 1999年第9期35-35,共1页
ALTHOUGH Chinese televisionworkers didn’t neglect children asan audience even in the early days oftelevision broadcasting in China,in thelate 1950’s,a lack of equipment confinedchildren’s programs to photographs an... ALTHOUGH Chinese televisionworkers didn’t neglect children asan audience even in the early days oftelevision broadcasting in China,in thelate 1950’s,a lack of equipment confinedchildren’s programs to photographs andpictures.These have become part of 展开更多
关键词 CI CL Rich pickings for Children
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USTC-Pickers:a Unified Set of seismic phase pickers Transfer learned for China 被引量:8
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作者 Jun Zhu Zefeng Li Lihua Fang 《Earthquake Science》 2023年第2期95-112,共18页
Current popular deep learning seismic phase pickers like PhaseNet and EQTransformer suffer from performance drop in China.To mitigate this problem,we build a unified set of customized seismic phase pickers for differe... Current popular deep learning seismic phase pickers like PhaseNet and EQTransformer suffer from performance drop in China.To mitigate this problem,we build a unified set of customized seismic phase pickers for different levels of use in China.We first train a base picker with the recently released DiTing dataset using the same U-Net architecture as PhaseNet.This base picker significantly outperforms the original PhaseNet and is generally suitable for entire China.Then,using different subsets of the DiTing data,we fine-tune the base picker to better adapt to different regions.In total,we provide 5 pickers for major tectonic blocks in China,33 pickers for provincial-level administrative regions,and 2 special pickers for the Capital area and the China Seismic Experimental Site.These pickers show improved performance in respective regions which they are customized for.They can be either directly integrated into national or regional seismic network operation or used as base models for further refinement for specific datasets.We anticipate that this picker set will facilitate earthquake monitoring in China. 展开更多
关键词 phase picking transfer learning model customization
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Benchmark on the accuracy and efficiency of several neural network based phase pickers using datasets from China Seismic Network 被引量:4
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作者 Ziye Yu Weitao Wang Yini Chen 《Earthquake Science》 2023年第2期113-131,共19页
Seismic phase pickers based on deep neural networks have been extensively used recently,demonstrating their advantages on both performance and efficiency.However,these pickers are trained with and applied to different... Seismic phase pickers based on deep neural networks have been extensively used recently,demonstrating their advantages on both performance and efficiency.However,these pickers are trained with and applied to different data.A comprehensive benchmark based on a single dataset is therefore lacking.Here,using the recently released DiTing dataset,we analyzed performances of seven phase pickers with different network structures,the efficiencies are also evaluated using both CPU and GPU devices.Evaluations based on F1-scores reveal that the recurrent neural network(RNN)and EQTransformer exhibit the best performance,likely owing to their large receptive fields.Similar performances are observed among PhaseNet(UNet),UNet++,and the lightweight phase picking network(LPPN).However,the LPPN models are the most efficient.The RNN and EQTransformer have similar speeds,which are slower than those of the LPPN and PhaseNet.UNet++requires the most computational effort among the pickers.As all of the pickers perform well after being trained with a large-scale dataset,users may choose the one suitable for their applications.For beginners,we provide a tutorial on training and validating the pickers using the DiTing dataset.We also provide two sets of models trained using datasets with both 50 Hz and 100 Hz sampling rates for direct application by end-users.All of our models are open-source and publicly accessible. 展开更多
关键词 neural network deep learning seismic phase picking earthquake detection open-source science
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Pick-up strategies for and electrical characterization of ZnO nanowires with a SEM-based nanomanipulator 被引量:1
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作者 Mei Liu Lingdi Kong +5 位作者 Weilin Su Aristide Djoulde Kai Cheng Jinbo Chen Jinjun Rao Zhiming Wang 《Nanotechnology and Precision Engineering》 CAS CSCD 2023年第1期26-31,共6页
Because of their unique mechanical and electrical properties,zinc oxide(ZnO)nanowires are used widely in microscopic and nanoscopic devices and structures,but characterizing them remains challenging.In this paper,two ... Because of their unique mechanical and electrical properties,zinc oxide(ZnO)nanowires are used widely in microscopic and nanoscopic devices and structures,but characterizing them remains challenging.In this paper,two pick-up strategies are proposed for characterizing the electrical properties of ZnO nanowires using SEM equipped with a nanomanipulator.To pick up nanowires efficiently,direct sampling is compared with electrification fusing,and experiments show that direct sampling is more stable while electrification fusing is more efficient.ZnO nanowires have cut-off properties,and good Schottky contact with the tungsten probes was established.In piezoelectric experiments,the maximum piezoelectric voltage generated by an individual ZnO nanowire was 0.07 V,and its impedance decreased with increasing input signal frequency until it became stable.This work offers a technical reference for the pick-up and construction of nanomaterials and nanogeneration technology. 展开更多
关键词 ZnO nanowires nanomanipulator picking up strategy piezoelectric properties electrical characterization
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MMP15、PICK1基因在山羊睾丸中的表达研究
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作者 汪艳妃 张艳 +1 位作者 惠茂茂 陈祥 《山地农业生物学报》 2023年第4期18-25,共8页
MMP15、PICK1基因均是哺乳动物性腺组织中影响性腺生理功能正常发挥的重要影响因子,但它们在黔北麻羊睾丸组织中的表达规律尚不明晰。为研究黔北麻羊不同月龄的睾丸中MMP15、PICK1基因的表达情况,本试验以黔北麻羊为研究对象,通过qRT-PC... MMP15、PICK1基因均是哺乳动物性腺组织中影响性腺生理功能正常发挥的重要影响因子,但它们在黔北麻羊睾丸组织中的表达规律尚不明晰。为研究黔北麻羊不同月龄的睾丸中MMP15、PICK1基因的表达情况,本试验以黔北麻羊为研究对象,通过qRT-PCR技术分别检测MMP15与PICK1基因在1、3、6、9、12月龄的黔北麻羊睾丸中的表达水平并建立基因表达的标准曲线。结果显示:MMP15、PICK1基因在黔北麻羊不同月龄睾丸组织中均有表达;MMP15基因在黔北麻羊不同月龄睾丸中的表达量从高到低排列为:12月龄>1月龄>9月龄>3月龄>6月龄;12月龄与1月龄的表达差异不显著(P>0.05),二者表达量均极显著高于3月龄、6月龄、9月龄(P<0.01);9月龄表达显著高于3月龄(P<0.05),极显著高于6月龄(P<0.01);3月龄表达极显著高于6月龄(P<0.01)。PICK1基因在黔北麻羊不同月龄睾丸中的表达量从高到低排列分别是:3月龄>6月龄>12月龄>9月龄>1月龄;3、6月龄其表达量极显著高于9、12月龄(P<0.01);1月龄与其他几个月龄相比,其表达量均极显著低于其他月龄(P<0.01)。结合黔北麻羊的生理机能发育情况,黔北麻羊在6月龄时已经基本达到性成熟,所以此时睾丸内细胞活动增强,睾丸机能活跃。本次试验结果表明,黔北麻羊睾丸生精功能的正常发挥或与MMP15及PICK1基因的表达量有关。 展开更多
关键词 黔北麻羊 MMP15 PICK1 相对表达量 睾丸
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Truncating PICK1 Variant Identified in Azoospermia Affected Mitochondrial Dysfunction in Knockout Mice
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作者 Yao-qiang DU Chong-yi SHU +11 位作者 Min ZHENG Wei-de XU Yue SUN Lu SHEN Chen ZHANG Yu-xin ZHANG Qian-ni WANG Kai-qiang LI Bing-yu CHEN Ke HAO Jian-xin LYU Zhen WANG 《Current Medical Science》 SCIE CAS 2023年第2期313-323,共11页
Objective The protein interacting with C kinase 1(PICK1)plays a critical role in vesicle trafficking,and its deficiency in sperm cells results in abnormal vesicle trafficking from Golgi to acrosome,which eventually di... Objective The protein interacting with C kinase 1(PICK1)plays a critical role in vesicle trafficking,and its deficiency in sperm cells results in abnormal vesicle trafficking from Golgi to acrosome,which eventually disrupts acrosome formation and leads to male infertility.Methods An azoospermia sample was filtered,and the laboratory detection and clinical phenotype indicated typical azoospermia in the patient.We sequenced all of the exons in the PICK1 gene and found that there was a novel homozygous variant in the PICK1 gene,c.364delA(p.Lys122SerfsX8),and this protein structure truncating variant seriously affected the biological function.Then we constructed a PICK1 knockout mouse model using clustered regularly interspaced short palindromic repeat cutting technology(CRISPRc).Results The sperm from PICK1 knockout mice showed acrosome and nucleus abnormalities,as well as dysfunctional mitochondrial sheath formation.Both the total sperm and motility sperm counts were decreased in the PICK1 knockout mice compared to wild-type mice.Moreover,the mitochondrial dysfunction was verified in the mice.These defects in the male PICK1 knockout mice may have eventually led to complete infertility.Conclusion The c.364delA novel variant in the PICK1 gene associated with clinical infertility,and pathogenic variants in the PICK1 may cause azoospermia or asthenospermia by impairing mitochondrial function in both mice and humans. 展开更多
关键词 PICK1 AZOOSPERMIA truncating variant knockout mice mitochondrial dysfunction
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Pregnancy outcomes following supplementation of single dose GnRH agonist to sustain the luteal phase in antagonist fresh embryo transfer cycles:A multicentric prospective cohort study
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作者 Tatapudi S V Parvathi Devi Alka Gahlot +3 位作者 Sangita Sharma Manisha Choudhary Ravikant Soni Meeta Sharma 《Asian pacific Journal of Reproduction》 2023年第4期162-169,共8页
Objective:To determine whether a single dose of gonadotropin-releasing hormone(GnRH)agonist administered subcutaneously in addition to the regular progesterone supplementation could provide a better luteal support in ... Objective:To determine whether a single dose of gonadotropin-releasing hormone(GnRH)agonist administered subcutaneously in addition to the regular progesterone supplementation could provide a better luteal support in antagonist protocol fresh embryo transfer cycles.Methods:This prospective,multicentric,cohort study included total 140 women,70 in each group.Controlled ovarian stimulation was carried out as per fixed GnRH antagonist protocol.The trigger was given with hCG.In vitro fertilization/intracytoplasmic sperm injection(IVF/ICSI)was performed and day-3 embryos were transferred.Patients were divided into groups 1 and 2 based on computer generated randomization sheet.Six days following oocyte retrieval,group 1 received 0.2 mg decapeptyl subcutaneously in addition to regular progesterone support while group 2 received progesterone only.Luteal support was given for 14 days to both groups;if pregnancy was confirmed luteal support was continued till 12 weeks of gestation.The clinical pregnancy rate was the primary outcome.The implantation rate,miscarriage rate,live birth delivery rate,and multiple pregnancy rates were the secondary outcomes.Results:A total of 140 patients were analysed,70 in each group.Clinical pregnancy rates(47.1%vs.35.7%;P=0.17),implantation rates(23.4%vs.18.1%,P=0.24),live birth delivery rates(41.4%vs.27.1%,P=0.08),and multiple pregnancy rates(21.2%vs.16.0%,P=0.74)were higher in group 1 than in group 2.Group 1 had a lower miscarriage rate than group 2(5.7%vs.8.6%;P=0.75).However,these differences were not statistically significant between the two groups.Conclusions:Administration of a single dose of GnRH agonist in addition to regular natural micronized vaginal progesterone as luteal support in GnRH antagonist protocol cycles marginally improves implantation rates,clinical pregnancy rates,and live birth delivery rates.However,more studies with higher sample sizes are needed before any conclusive statements about GnRH agonist as luteal phase support can be made. 展开更多
关键词 GnRH agonist TRIPTORELIN IVF Ovum pick up Luteal phase support Antagonist protocol Cleavage stage Fresh embryo transfer Live birth delivery rate
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Flower Characteristics and Resource Evaluation of Fruit Mulberry
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作者 Lianjun WANG 《Asian Agricultural Research》 2023年第5期44-45,48,共3页
[Objectives]In order to study the flower characteristics of mulberry and evaluate the resources of fruit mulberry.[Methods]Firstly,the resources of fruit mulberry were collected,and four varieties of fruit mulberry(‘... [Objectives]In order to study the flower characteristics of mulberry and evaluate the resources of fruit mulberry.[Methods]Firstly,the resources of fruit mulberry were collected,and four varieties of fruit mulberry(‘Dashi’mulberry,‘Changguo’milk mulberry,‘agate’mulberry and‘Xiangjin’milk mulberry)were collected.Then,the flower characteristics,fruit quality,and adaptability of fruit mulberry were studied.[Results]Mulberry is a dioecious plant,and the inflorescences are lurocatkin.The male flowers will fall off automatically,while the female flowers will develop into fruit.The four varieties of fruit mulberry have good adaptability in Shanghai,and their fruits have their own characteristics.At the same time,in order to use the resources of fruit mulberry,mulberry fruit picking activities were carried out for many times.[Conclusions]This study provides a reference for the cultivation,collection,utilization and evaluation of mulberry. 展开更多
关键词 Fruit mulberry Flower characteristics ADAPTABILITY PICKING
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Niemann-Pick A/B Disease in a 13-Year-Old Child and Review of the Literature
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作者 Issam Taha Ilham Tadmori Moustapha Hida 《Open Journal of Pediatrics》 2023年第6期907-913,共7页
Niemann-Pick disease (NPD) refers to a group of patients who have varying degrees of lipid storage and foam cell infiltration in tissues, as well as overlapping clinical features, including hepatosplenomegaly, insuffi... Niemann-Pick disease (NPD) refers to a group of patients who have varying degrees of lipid storage and foam cell infiltration in tissues, as well as overlapping clinical features, including hepatosplenomegaly, insufficiency pulmonary and/or central nervous system (CNS). Thanks to the pioneering work of Roscoe Brady and colleagues, we now know that there are two distinct metabolic abnormalities that explain NPD. The first is due to the deficient activity of the acid sphingomyelinase enzyme (ASM;NPD “types A and B”), and the second is due to defective functioning in the transport of cholesterol (NPD “type C”). We report the case of a 13-year-old adolescent diagnosed with Niemann-Pick A/B disease. 展开更多
关键词 Nieman Pick SPLENOMEGALY HEPATOMEGALY Enzymatic Test
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PICK1参与调控芬太尼诱导的大鼠痛觉过敏机制的研究
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作者 金宇 《生物化工》 CAS 2023年第6期6-9,39,共5页
目的:探讨与蛋白激酶C相互作用的蛋白质1(PICK1)参与调控阿片类药物诱导的痛觉过敏(OIH)的分子机制。方法:颈部皮下注射枸橼酸芬太尼建立大鼠OIH模型,通过PCR实验检测Pick1在大鼠背根神经节(DRG)中表达水平;构建Pick1的过表达载体,包装p... 目的:探讨与蛋白激酶C相互作用的蛋白质1(PICK1)参与调控阿片类药物诱导的痛觉过敏(OIH)的分子机制。方法:颈部皮下注射枸橼酸芬太尼建立大鼠OIH模型,通过PCR实验检测Pick1在大鼠背根神经节(DRG)中表达水平;构建Pick1的过表达载体,包装pLV-Pick1重组慢病毒后对大鼠进行鞘内注射,电生理测定大鼠DRG神经元兴奋性变化,观察慢病毒注射前(T0)、慢病毒表达后(T1)和芬太尼给药后(T2)大鼠机械痛阈。结果:与Control组相比,OIH组大鼠的机械痛阈明显降低;Pick1在OIH大鼠DRG中表达量有明显下调;芬太尼孵育后DRG细胞的动作电位的阈电流明显降低,鞘内注射pLV-Pick1慢病毒的大鼠DRG细胞在芬太尼孵育后,阈电流相无显著下调。结论:在DRG水平上,Pick1可参与调控痛觉过敏,在DRG上干涉Pick1的表达,可以缓解芬太尼诱导的痛觉过敏。 展开更多
关键词 背根神经节 Pick1 痛觉过敏 芬太尼
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AMPA受体和相关蛋白在束缚应激大鼠相关脑区的表达变化及逍遥散对其影响 被引量:11
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作者 岳广欣 王竹风 +4 位作者 张巧丽 赵歆 岳利峰 丁杰 陈家旭 《中国应用生理学杂志》 CAS CSCD 北大核心 2008年第2期129-132,共4页
目的:观察海马及杏仁核α-氨基羟甲基恶唑丙酸(AMPA)受体亚基和相关调节蛋白在束缚应激状态下蛋白表达变化及逍遥散的调节作用。方法:使用每天捆绑3 h的方法制作慢性束缚应激动物模型,并用逍遥散进行干预,分别于7 d后和21 d后用Western ... 目的:观察海马及杏仁核α-氨基羟甲基恶唑丙酸(AMPA)受体亚基和相关调节蛋白在束缚应激状态下蛋白表达变化及逍遥散的调节作用。方法:使用每天捆绑3 h的方法制作慢性束缚应激动物模型,并用逍遥散进行干预,分别于7 d后和21 d后用Western blot方法检测各组大鼠海马CA1区、CA3区、齿状回(DG)和杏仁核的AMPA受体亚基GluR2/3及N-乙基顺丁烯二酰亚胺敏感性的融合蛋白(NSF)、PKC作用蛋白1(PICK1)蛋白表达的情况。结果:7 d应激可使DG和杏仁核的GluR2/3、NSF表达显著降低(P均<0.05),使PICK1在CA1区的表达量显著增多(P<0.05),逍遥散对PICK1变化显示出一定调节作用。21 d应激可使CA1区的GluR2/3、NSF表达升高,其中GluR2/3有显著性差异(P<0.01),而在杏仁核表达有降低趋势,逍遥散对其均有显著调节作用(均为P<0.05),21 d应激使杏仁核PICK1表达量出现升高趋势,逍遥散可显著降低其表达(P<0.05)。结论:AMPA受体在短期重复应激和慢性应激状态下反应不同,海马和杏仁核反应相反,逍遥散对慢性应激状态下AMPA受体表达的调节作用较短期重复应激强。 展开更多
关键词 束缚应激 突触可塑性 Western blot AMPA受体 NSF PICK1 逍遥散
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实行BOPS模式是否总是有益的?与传统双渠道的对比研究 被引量:38
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作者 刘咏梅 周笛 《运筹与管理》 CSSCI CSCD 北大核心 2018年第2期168-177,共10页
针对企业线上线下渠道融合战略中提出的BOPS(buy-online-and-pick-up-in-store)模式,在集中式和分散式两情境下分别构建并对比了BOPS和传统双渠道模型,探讨了在传统双渠道基础之上引入BOPS服务是否对企业而言总是有好处的,并分析了传统... 针对企业线上线下渠道融合战略中提出的BOPS(buy-online-and-pick-up-in-store)模式,在集中式和分散式两情境下分别构建并对比了BOPS和传统双渠道模型,探讨了在传统双渠道基础之上引入BOPS服务是否对企业而言总是有好处的,并分析了传统型消费者占比和消费者的服务敏感性程度对企业实施BOPS的影响。研究表明,企业是否在传统双渠道模式基础上增设BOPS服务,取决于市场中传统型消费者占比规模(即偏好传统零售的消费者)以及各类型消费者对线下服务的敏感性程度;BOPS模式下,当消费者BOPS行为的真正动机是出于欲体验线下零售服务时,对实施BOPS的制造商企业而言更为有利;同时,企业的产品定价和线下服务水平不仅需要考量生产、服务成本,同样需对消费者的服务敏感性有准确的了解和定位。 展开更多
关键词 BUY ONLINE and PICK up in STORE 渠道整合 传统双渠道 服务
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圆头精子症患者的遗传学检测与成功辅助育孕 被引量:4
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作者 郑灵燕 袁萍 +4 位作者 张宁峰 梁中锟 王文军 张清学 杨冬梓 《中山大学学报(医学科学版)》 CAS CSCD 北大核心 2015年第6期955-960,共6页
【目的】分析圆头精子症患者的遗传学发病机制。【方法】回顾分析1例圆头精子症患者,对其进行多种遗传学方法的检测,包括染色体检查、Y染色体微缺失基因检测及通过array-CGH芯片技术和Sanger测序筛查候选基因DPY19L2、PICK1、SPATA16。... 【目的】分析圆头精子症患者的遗传学发病机制。【方法】回顾分析1例圆头精子症患者,对其进行多种遗传学方法的检测,包括染色体检查、Y染色体微缺失基因检测及通过array-CGH芯片技术和Sanger测序筛查候选基因DPY19L2、PICK1、SPATA16。【结果】该患者在我中心行两个卵胞浆内单精子显微注射(ICSI)周期后,其妻孕足月剖宫产一对异卵双胞胎女儿。患者染色体核型为46,XY、Y染色体微缺失基因检测未见异常,array-CGH芯片检测排除了DPY19L2、SPATA16和PICK1基因及其他该芯片所涵盖区域的100kb以上的片段重复或缺失。Sanger测序PICK1、SPATA16基因也未发现点突变和小片段插入缺失等病理性改变。【结论】圆头精子症患者可通过ICSI方式获得健康后代,本研究排除了已知的候选基因DPY19L2、PICK1、SPATA16与该圆头精子症患者的致病相关,可能存在其他致病基因。 展开更多
关键词 圆头精子 卵胞浆内单精子显微注射 DPY19L2 PICK1 SPATA16
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PICK1蛋白生理功能及其作为药物新靶点研究进展 被引量:2
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作者 段贤春 汪永忠 +9 位作者 高家荣 冯艺戎 李翔 吴健 刘晓闯 魏良兵 何云娇 朱红 李瑛 夏伦祝 《中国药理学通报》 CAS CSCD 北大核心 2013年第11期1606-1610,共5页
PICK1蛋白(protein interacting with C alpha kinase 1)是一种同时具有PDZ和BAR区域的支架蛋白,在哺乳动物体内与多种蛋白质相互作用,并被证明在多种生理过程中发挥重要的调节作用,同时参与了多种疾病病理过程。因此,PICK1蛋白可能成... PICK1蛋白(protein interacting with C alpha kinase 1)是一种同时具有PDZ和BAR区域的支架蛋白,在哺乳动物体内与多种蛋白质相互作用,并被证明在多种生理过程中发挥重要的调节作用,同时参与了多种疾病病理过程。因此,PICK1蛋白可能成为极具前景的疾病治疗靶点。该文通过对近年来国内外发表的相关文献进行整理与分析,综述了PICK1蛋白的生理功能与其作为药物靶点的研究新进展,旨在为PICK1蛋白的深入研究提供理论支持。 展开更多
关键词 PICK1 PDZ结构域 生理功能 中风 脑病 药物靶点
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EphB4/ephrinB2逆向信号对RAW264.7破骨细胞分化中PDZ结构域蛋白表达变化的研究 被引量:2
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作者 毛英杰 黄旭 +2 位作者 赵鹃 史月华 谷志远 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2011年第5期464-472,共9页
Eph受体是酪氨酸蛋白激酶受体家族中最大的亚家族,ephrin(Eph受体相互作用蛋白)是其配体,它们是膜结合蛋白,相互依赖进行信号转导.内居蛋白(syntenin)与Pick1属于PDZ结构域(PSD-95/Dlg-/Zo-1 domain)蛋白,报道称能与ephrinB配体结合,但... Eph受体是酪氨酸蛋白激酶受体家族中最大的亚家族,ephrin(Eph受体相互作用蛋白)是其配体,它们是膜结合蛋白,相互依赖进行信号转导.内居蛋白(syntenin)与Pick1属于PDZ结构域(PSD-95/Dlg-/Zo-1 domain)蛋白,报道称能与ephrinB配体结合,但是否受Eph受体调控尚未见报道.以RAW264.7细胞株为研究对象,通过蛋白质印迹及/或免疫荧光分析显示RAW264.7细胞经RANKL诱导的破骨细胞表达ephrinB2、内居蛋白(syntenin)和Pick1三个蛋白质.将提前成簇的可溶性EphB4蛋白加入培养液,与ephrinB2配体结合,用来研究EphB4/ephrinB2逆向信号对syntenin和Pick1表达水平变化的影响.免疫印迹及Real-time RT-PCR分析结果显示,在EphB4-Fc实验组中Pick1的蛋白质及mRNA水平都有明显增加,然而在EphB4-Fc实验组与Fc对照组别间syntenin的蛋白质及mRNA水平未见明显变化.免疫共沉淀结果显示,syntenin和Pick1不能与ephrinB2共沉淀.以上结果初步探索了体外破骨细胞分化过程中,EphB4/ephrinB2逆向信号对PDZ结构域蛋白(ephrinB2配体潜在的下游信号分子)表达变化的调控. 展开更多
关键词 EPHB4 EPHRINB2 内居蛋白(syntenin) Pick1 RAW264.7细胞株
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