A pico-HPLC-LIF system for the amplification-free determination of multiple miRNAs in cells

MicroRNAs are a class of important biomarkers,and the simultaneous detection of multiple miRNAs can provide valuable information about many diseases and biological processes.Amplification-free determination has been developed for the analysis of multiple miRNAs because of its characteristic low cost and high fidelity.Herein,a method for the amplification-free analysis and simultaneous detection of multiple miRNAs based on a so-called pico-HPLC-LIF system is described.In this process,a bare open capilla ry with an inner diameter of 680 nm is used as a sepa ration column for a sample volume of several hundreds of femtoliters(300 fL),followed by separation and detection.The technique has a zeptomolar limit of detection.The method was applied to detect cellular miRNA from adenocarcinomic human alveolar basal epithelial(A549)cell extracts,and the simultaneous detection of the mir-182,miR-155,and let-7 a was achieved.The results showed that the expression of mir-182 and miR-155 was up-regulated and that of let-7 a was down-regulated in A549 cells.This method for multiple miRNAs detection is expected to have broad applications in miRNA-based disease diagnosis,prognosis,treatment,and monitoring.

HPLC Pico－Tag法分析人胎盘的氨基酸成分

ＨＰＬＣＰｉｃｏ－Ｔａｇ法分析人胎盘的氨基酸成分陈柏林，郭敏，陈莉娟（四川农业大学动物营养研究所，雅安６２５０１４）ＴｈｅＨＰＬＣＰｉｃｏ－ＴａｇＡｎａｌｙｓｉｓｏｆＡｍｉｎｏＡｃｉｄｓｉｎＰｌａｃｅｎｔａ￥ＣｈｅｎＢｏｌｉｎ；ＧｕｏＭｉｎ；Ｃｈｅｎ...

鸡肠中氨基酸含量的高效液相色谱分析

本文采用以异硫氰酸苯酯（ＰＩＴＣ）为柱前衍生化的高效液相色谱法测定氨基酸，并对方法测定条件进行了研究，得出流动相ｐＨ６．４０时测定的ＲＳＤ值最低，为０．５９％，样品经微波水解７ｍｉｎ（３００Ｗ功率），且稀释液加入５ｍｉｎ后进样方能得到最佳结果。用本法对鸡肠中氨基酸进行了分析，必需氨基酸总量占３４．９％，具有可开发利用之价值。

改良的PICO—TAG方法用于复方氨基酸制剂的成份分析

本文应用改良的PICO—TAG柱前衍生法,以HPLC替代氨基酸专用分析仪对复方氨基酸制剂肝醒灵注射液进行成份分析,经PICO—TAG氨基酸分析柱,在UV254nm处检测出该注射液含6种氨基酸成份,它们是Asp(5.41％)、Glu(19.28％)、Arg(31.22％)、Val(13.42％)、Ile(13.36％)、Leu(21.03％)。方法精确、省时。