Effects of Diet with Picria fel-tarrae Lour Particle on the Growth Performance, Blood Biochemical Indexes, Immune and Antioxidant Function of Hongguang Yellow Chickens

[Objectives]To investigate the effect of diet with Picria fel-tarrae Lour particle(PFLp)on growth performance,blood biochemical indexes,immune and antioxidant function of Hongguang yellow chickens(HYC).[Methods]80 HYC in age of 1 d were divided into 4 groups randomly and each group was assigned to 5 replicates with 24 chicks in each replicate.Group A was control group fed only basal diet.Group B,C and D were treatment groups and fed diets with 0.125%,0.25%and 0.50%of PFLp respectively during the trial of 49 d.Serum samples were taken from 10 chicks in each group in the age of 21,35 and 49 d respectively and analyzed for biochemical,immune and antioxidant parameters in blood.[Results]ADG from group C in 21 d was increased significantly than that from group A,B and D(P<0.01),and ADG in 35 d from group C was also significantly greater than that from group A,B and D(P<0.05),but not much difference was found on ADG among the 4 groups in 49 d(P>0.05);ADFI from group C in 35 d was significantly higher than that from group A,B and D(P<0.05).ADFI from group C in 21 and 49 d were also higher than that from group A(P>0.05).FCR from group C in 21 d was much better than that from the other three groups(P<0.01).Death rate of group C was 50 percent lower than group A;There was no difference occurred in serum biochemical and immune parameters among the 4 groups during the trial(P>0.05).However,the T-AOC from group C and D in 21 d was enhanced significantly than that from group A and B(P<0.01),and the one from group C in 35 d was also increased greatly than that from group A,B and D(P<0.05).GSH from group C and D in 21 d was much higher than that from group A and B(P<0.05),and the ones from group C and D in 35 d were also significantly higher than those from group A and B(P<0.01).T-SOD in three stages trended up also in group C and D(P>0.05).[Conclusions]The diet with 0.25%PFLp in 21 and 35 d could dramatically improve the growth performance,significantly increase the antioxidant capacity and effectively reduce death rate.Diet with 0.25%PFLp is the most appropriate among the three different additions.

Effects of Dietary Picria felterrae Lour Extracts on Serum Biochemical Indexes and Immune Function of Guangxi Partridge Chickens

[Objective] This trial was to investigate the effect of dietary Picria felterrae Lour extracts on serum biochemical indexes and immune function of Guangxi Partridge chickens. [Method] Totally 500 individuals of 1-day-old Guangxi Partridge chickens were randomly divided into five groups, five replicates each group and 20 chicks each replicate. Groups A, B and C were treatment groups supplemented with 0.70%, 0.35% and0.175% P. felterrae extracts in the basal diet, respectively; group D was a medical control group supplemented with 0.01% colistin sulfate premix in the basal diet; and group E was a control group with basal diet. Serum samples were collected from 10 chickens in each group at 21, 35 and 49 days of age, to analyze the biochemical and immune indexes. [Result] It had no significant impact on serum TP and GLU contents of chickens by adding different levels of P. felterrae extracts in the basal diet（P 〉0.05）. However, at 21 and 35 days of age, the TBILI content of chickens in groups A, B and C were extremely lower or significantly lower than that in group E（ P〈0.01; P〈0.05）. At 35 days of age, the serum AKP activities in groups A, B and C were extremely higher than that in group E（P〈0.01）, and the serum GOT activities at 21 and 35 days of age in group C were significantly higher than that in group E（P〈0.05）. At 49 days of age, the serum Ig G content in groups A, B and C and the IL-2 content in group B and group C were significantly lower or extremely lower than that in group E（ P〈0.05; P〈0.01）; the T-AOC content in group B was significantly higher than that in group E（P〈0.05）; the GSH content in groups A, B and C were significantly higher than that in group E（ P〈0.05）. Adding different levels of P. felterrae extracts in the diet significantly decreased the TBILI content, reduced the Ig G, IL-2 and IL-6 contents, and improved the AKP and GOT activities and the T-AOC and GSH contents of Guangxi Partridge chicken. [Conclusion] Adding 0.35% P. felterrae extracts in the basal diet received the best effort.

多指标综合评分法优选苦玄参皂苷提取工艺

目的:确定提取苦玄参皂苷的工艺条件。方法:采用正交试验法,以苦玄参总苷和苦玄参苷ⅠA、ⅠB的含量为考察指标进行提取工艺优选。结果:最佳提取工艺为:用20倍量70%乙醇浸泡3h,于80℃回流提取2次,提取用时第一次3h,第二次1h。结论:优选出的工艺简便,实用,准确度高。该工艺可用于生产参考。

苦玄参提取物对仔猪生长性能的影响研究

[目的]试验旨在探讨苦玄参提取物对仔猪生长性能的影响,为其在动物生产中更好地应用提供参考依据。[方法]选取体重相近的28～35日龄杜×长×大三元杂交的健康断奶仔猪250头,随机分为5组(A、B、C、D、E组),每组设5个重复,每个重复10头。其中,A、B、C组依次为苦玄参提取物高、中、低3个剂量试验组,分别在基础日粮中添加3.500、1.750和0.875 g/kg苦玄参提取物;D组在基础日粮中添加对照药物七补散4.0 g/kg;E组为空白对照组,饲喂基础日粮,预饲期7 d,正试期30 d。于试验正式开始的第0天、第15天和第30天7:00分别对各组的每一头试验猪进行空腹称重,并每天记录每个组试验猪的采食量,计算平均日采食量(ADFI)、平均日增重(ADG)和料重比(F/G)。[结果]添加苦玄参提取物后,A、B、C组仔猪的体重、ADG及ADFI,与E组相比显著或极显著(P<0.05或P<0.01)增加,而料重比降低;其中苦玄参提取物中剂量组仔猪的体重、平均日增重及平均日采食量提升最明显,料重比也极显著低于D组与E组(P<0.01)。[结论]苦玄参提取物对仔猪生长具有一定的促进作用,以1.750 g/kg的添加剂量添加在饲料中效果最佳。

苦玄参提取物对广西麻鸡生长性能及免疫器官指数的影响

试验旨在研究常规日粮添加苦玄参提取物对广西麻鸡生长性能及免疫器官指数的影响。选用1日龄广西麻鸡500羽,随机分5组,A、B和C组为试验组,在基础日粮中分别添加0.70%、0.35%和0.175%的苦玄参提取物;D组为阳性药物对照组,添加0.01%硫酸粘菌素预混料;E组为空白对照组,饲喂基础日粮。试期105d,期间于第21、35和49日龄时分别从每组抽取5羽宰杀,测定免疫器官指数。结果表明:日粮添加0.175%的苦玄参提取物可在一定程度上提高广西麻鸡的平均日采食量,也明显提高其生长性能和21日龄的法氏囊指数。

苦玄参提取物对仔猪血清生化指标及免疫指标的影响

试验旨在探讨苦玄参提取物对仔猪血液生化指标、抗氧化功能和部分免疫指标的影响,为苦玄参提取物在仔猪生产中的合理利用提供理论依据。选取28~35日龄、体重相近的杜×长×大三元杂交断奶仔猪250头,随机分为5组(A、B、C、D、E组),每组5个重复,每个重复10头。其中A、B、C组依次为苦玄参提取物高、中、低剂量组,分别在基础日粮中添加3.5、1.75和0.875g/kg苦玄参提取物;D组为药物对照组,在基础日粮中添加4.0g/kg七补散;E组为空白对照组,饲喂基础日粮。预饲期7d,正试期30d。分别在用药14d后和停药14d后时,从每组中随机选取接近平均体重的10头仔猪于晨饲前进行前腔静脉采血,测定血清生化指标、抗氧化指标和免疫指标。结果显示:(1)各组仔猪血液生化指标差异均不显著(P>0.05),说明苦玄参提取物对仔猪体内糖代谢、脂代谢及消化系统无显著影响;(2)从抗氧化指标看,添加苦玄参提取物14d后,B组一氧化氮(NO)含量极显著高于其他组(P<0.01),A组总抗氧化能力(T-AOC)极显著高于其他组(P<0.01),B、D组显著或极显著高于C、E组(P<0.05;P<0.01);而停药14d后,E组谷胱甘肽(GSH)含量显著高于B、C、D组,各组其余指标差异均不显著(P>0.05);(3)从免疫指标看,添加苦玄参提取物14d后,B组免疫球蛋白G (IgG)含量极显著高于其他组(P<0.01),A组白细胞介素6(IL-6)含量显著高于B、D、E组(P<0.05);而停药14d后,各组免疫指标差异不显著(P>0.05);综上所述,日粮中添加0.875~3.5g/kg苦玄参提取物对仔猪无不良影响,且可在一定程度上增强机体的抗氧化功能和机体免疫水平。