[Objective] The aim of this study was to improve the purification and protective potency of HP-PRRS inactivated vaccine. [Method] HP-PRRS virus that had been multiplied inside Marc-145 cells was collected and concentr...[Objective] The aim of this study was to improve the purification and protective potency of HP-PRRS inactivated vaccine. [Method] HP-PRRS virus that had been multiplied inside Marc-145 cells was collected and concentrated 50 times and then inactivated. Complete virions were separated and collected by chromatography with Sepharose 4 Fast Flow. Oil adjuvant was added to prepare purified inactivated vaccine. [Result] Viral protein was separated from other proteins by purification and the viral protein contents ranged from 76.7% to 82.4%, and 96% of the expected serum proteins were removed. Protective potency of purified vaccine was above 4/5 and positive conversion rate of antibody was over 86%, both higher than that of unpurified vaccine. The differences were significant. [Conclusion] The experiment il-lustrated that the immune efficacy of vaccine can be enhanced through concentrat- ing and purifying, while the non-viral protein can be removed, so that allergic reaction and stress response cadsed by vaccine inoculation can be avoided.展开更多
目的比较国产腮腺炎单价疫苗与进口含腮腺炎成分的联合疫苗(MMR)在免疫原性的差异,并系统分析国产腮腺炎单价疫苗保护效果,为免疫规划工作提供科学依据。方法检索中国生物医学文献光盘数据库、中国期刊全文数据库、维普中文科技期刊、...目的比较国产腮腺炎单价疫苗与进口含腮腺炎成分的联合疫苗(MMR)在免疫原性的差异,并系统分析国产腮腺炎单价疫苗保护效果,为免疫规划工作提供科学依据。方法检索中国生物医学文献光盘数据库、中国期刊全文数据库、维普中文科技期刊、万方数字化期刊、Pubmed、EMbase、Web of Science等数据库,筛选符合研究目的的文献,对符合纳入标准的文章采用Stata10.0进行Meta分析。结果提取8篇关于含腮腺炎疫苗成分的国产单价疫苗与进口联合疫苗接种后抗体阳转情况比较的文献资料,分析结果显示:国产单价疫苗和进口MMR组接种后腮腺炎抗体阳性率差异无统计学意义[RR=1.01,95%CI(0.95,1.07),P=0.780];入选的8篇文章均比较了国产单价疫苗的接种组和未接种组的保护效果的研究,合并后接种组腮腺炎发病率和未接种组腮腺炎发病率差异有统计学意义[OR=0.16,95%CI(0.06,0.45),P<0.001],接种国产单苗发病率显著低于未接种组,其疫苗保护率为84%。结论国产单价疫苗在免疫原性方面和进口MMR无统计学差异,国产单价腮腺炎疫苗对预防儿童腮腺炎有保护作用。展开更多
为提高目前高致病性蓝耳病(HR-PRRS)灭活疫苗的免疫效果,将经Marc-145细胞培养收获的HP-PRRS病毒原液超滤浓缩50倍,再经灭活后用Sepharose 4 Fast Flow分子筛柱层析,收集完整的病毒粒子,加油佐剂制成纯化HP-PRRS灭活疫苗。纯化后疫苗的...为提高目前高致病性蓝耳病(HR-PRRS)灭活疫苗的免疫效果,将经Marc-145细胞培养收获的HP-PRRS病毒原液超滤浓缩50倍,再经灭活后用Sepharose 4 Fast Flow分子筛柱层析,收集完整的病毒粒子,加油佐剂制成纯化HP-PRRS灭活疫苗。纯化后疫苗的攻毒保护率达到4/5以上,血清抗体检测阳转率达86%以上,均高于同批病毒液制成的未纯化灭活疫苗,且差异显著。试验证明,HR-PRRS病毒液经浓缩纯化制成疫苗后,增强了免疫效果。展开更多
基金Supported by Science and Technical Development Plan of Jilin City(2013210029)Fund for Supporting Key Subjects in Jilin Agricultural Science and Technology College(2013x023)~~
文摘[Objective] The aim of this study was to improve the purification and protective potency of HP-PRRS inactivated vaccine. [Method] HP-PRRS virus that had been multiplied inside Marc-145 cells was collected and concentrated 50 times and then inactivated. Complete virions were separated and collected by chromatography with Sepharose 4 Fast Flow. Oil adjuvant was added to prepare purified inactivated vaccine. [Result] Viral protein was separated from other proteins by purification and the viral protein contents ranged from 76.7% to 82.4%, and 96% of the expected serum proteins were removed. Protective potency of purified vaccine was above 4/5 and positive conversion rate of antibody was over 86%, both higher than that of unpurified vaccine. The differences were significant. [Conclusion] The experiment il-lustrated that the immune efficacy of vaccine can be enhanced through concentrat- ing and purifying, while the non-viral protein can be removed, so that allergic reaction and stress response cadsed by vaccine inoculation can be avoided.
文摘目的比较国产腮腺炎单价疫苗与进口含腮腺炎成分的联合疫苗(MMR)在免疫原性的差异,并系统分析国产腮腺炎单价疫苗保护效果,为免疫规划工作提供科学依据。方法检索中国生物医学文献光盘数据库、中国期刊全文数据库、维普中文科技期刊、万方数字化期刊、Pubmed、EMbase、Web of Science等数据库,筛选符合研究目的的文献,对符合纳入标准的文章采用Stata10.0进行Meta分析。结果提取8篇关于含腮腺炎疫苗成分的国产单价疫苗与进口联合疫苗接种后抗体阳转情况比较的文献资料,分析结果显示:国产单价疫苗和进口MMR组接种后腮腺炎抗体阳性率差异无统计学意义[RR=1.01,95%CI(0.95,1.07),P=0.780];入选的8篇文章均比较了国产单价疫苗的接种组和未接种组的保护效果的研究,合并后接种组腮腺炎发病率和未接种组腮腺炎发病率差异有统计学意义[OR=0.16,95%CI(0.06,0.45),P<0.001],接种国产单苗发病率显著低于未接种组,其疫苗保护率为84%。结论国产单价疫苗在免疫原性方面和进口MMR无统计学差异,国产单价腮腺炎疫苗对预防儿童腮腺炎有保护作用。
文摘为提高目前高致病性蓝耳病(HR-PRRS)灭活疫苗的免疫效果,将经Marc-145细胞培养收获的HP-PRRS病毒原液超滤浓缩50倍,再经灭活后用Sepharose 4 Fast Flow分子筛柱层析,收集完整的病毒粒子,加油佐剂制成纯化HP-PRRS灭活疫苗。纯化后疫苗的攻毒保护率达到4/5以上,血清抗体检测阳转率达86%以上,均高于同批病毒液制成的未纯化灭活疫苗,且差异显著。试验证明,HR-PRRS病毒液经浓缩纯化制成疫苗后,增强了免疫效果。
