The competition between Bidens pilosa and Setaria viridis alters soil microbial composition and soil ecological function

Bidens pilosa is recognized as one of the major invasive plants in China.Its invasion has been associated with significant losses in agriculture,forestry,husbandry,and biodiversity.Soil ecosystems play an important role in alien plant invasion.Microorganisms within the soil act as intermediaries between plants and soil ecological functions,playing a role in regulating soil enzyme activities and nutrient dynamics.Understanding the interactions between invasive plants,soil microorganisms,and soil ecological processes is vital for managing and mitigating the impacts of invasive species on the environment.In this study,we conducted a systematic analysis focusing on B.pilosa and Setaria viridis,a common native companion plant in the invaded area.To simulate the invasion process of B.pilosa,we constructed homogeneous plots consisting of B.pilosa and S.viridis grown separately as monocultures,as well as in mixtures.The rhizosphere and bulk soils were collected from the alien plant B.pilosa and the native plant S.viridis.In order to focus on the soil ecological functional mechanisms that contribute to the successful invasion of B.pilosa,we analyzed the effects of B.pilosa on the composition of soil microbial communities and soil ecological functions.The results showed that the biomass of B.pilosa increased by 27.51% and that of S.viridis was significantly reduced by 66.56%.The organic matter contents in the bulk and rhizosphere soils of B.pilosa were approximately 1.30 times those in the native plant soils.The TN and NO_(3)^(-)contents in the rhizosphere soil of B.pilosa were 1.30 to 2.71 times those in the native plant soils.The activities of acid phosphatase,alkaline phosphatase,and urease in the rhizosphere soil of B.pilosa were 1.98-2.25 times higher than in the native plant soils.Using high-throughput sequencing of the16S rRNA gene,we found that B.pilosa altered the composition of the soil microbial community.Specifically,many genera in Actinobacteria and Proteobacteria were enriched in B.pilosa soils.Further correlation analyses verified that these genera had significantly positive relationships with soil nutrients and enzyme activities.Plant biomass,soil p H,and the contents of organic matter,TN,NO_(3)^(-),TP,AP,TK,and AK were the main factors affecting soil microbial communities.This study showed that the invasion of B.pilosa led to significant alterations in the composition of the soil microbial communities.These changes were closely linked to modifications in plant traits as well as soil physical and chemical properties.Some microbial species related to C,N and P cycling were enriched in the soil invaded by B.pilosa.These findings provide additional support for the hypothesis of soil-microbe feedback in the successful invasion of alien plants.They also offer insights into the ecological mechanism by which soil microbes contribute to the successful invasion of B.pilosa.Overall,our research contributes to a better understanding of the complex interactions between invasive plants,soil microbial communities,and ecosystem dynamics.

Antidiabetic Properties of Bidens pilosa and Its Polyacetylenic Compounds for Management of Diabetes: Systematic Review

Bidens pilosa is a member of the Asteraceae family that is widely distributed across the tropics. It has been utilized by different communities both as food and medicinal herb. This plant and its polyacetylenic compounds hold potential as a natural antidiabetic intervention that can be used to combat this global public health problem. Bioactive compounds found in this plant constitute promising interventions for combating obesity which is a major risk factor for the development of type 2 diabetes. These phytocompounds can work independently or synergistically to modulate appetite, lipase activity, adipogenesis and adipocyte apoptosis. However, the efficacy, mode of action and scope of management of diabetes by these compounds remains elusive. The current review aims to summarize data on efficacy in the management of diabetes, an antidiabetic candidate polyacetylenic compound and possible biological activities as an antidiabetic agent from the available literature. Much emphasis has been directed to cytopiloyne as a representative of polyacetylenic compounds extracted from Bidens pilosa and its activity on diabetic animal models. The majority of the studies conducted on animal models described antidiabetic mechanisms that range from hypoglycemic to secretagogue activity of cytopiloyne in a dose-dependent manner. A clinical trial pilot indicated improved glycemic control of Bidens pilosa formulation among diabetic patients in the study. Bidens pilosa and its compounds are highly potent antidiabetic agent(s) that should be graduated to an intervention for management of diabetes through pre-clinical and clinical trials to elucidate its efficacy and safety.

Accumulation behavior of heavy metals by Bidens pilosa L.from metallurgical slag:effects on plant physiology and absorption characteristics

Many of the abandoned mining and industrial land in villages and towns are seriously polluted by heavy metals in China,it is necessary for sustainable development to adopt efficient and economical ways to restore the ecology of abandoned mining and industrial land.Pollution level of topsoil contaminated with metallurgical slag from nonferrous metal smelting waste site in Baoding,North China and the heavy metals(HMs)accumulation behavior of Bidens pilosa L.(B.pilosa,native pioneer plant)were studied.Two selected study sites were mainly contaminated by As(270~434 mg/kg),Cd(63~95 mg/kg),Pb(5496~24504 mg/kg)and Zn(4500~21300 mg/kg),which exceed the national standard severely.Investigation of multi-metal accumulation in different parts of B.pilosa indicated that the absorption of toxic metals varied by types,concentration and species of HMs under stress conditions,soil property and plant tissues.The results showed that B.pilosa had excellent ability to accumulate HMs under different HMs stress condition,with the highest accumulation concentration of 85 mg/kg for As,380 mg/kg for Cd,4000 mg/kg for Pb,and 7500 mg/kg for Zn in roots under experimental conditions,respectively.The growth trend of B.pilosa declined with the increase of HMs stress concentration in tested soils.HMs stress led to different degrees of plant toxicity and obstruction of physiological metabolism.Among the plant physiological index,Chla and ChlT decreased 28.0%and 28.1%,37.3%and 35.5%under different stress treatments,respectively.Indicators related to physiological metabolic strength and stress resistance of plant,such as MDA(Malondiadehyde),CAT(catalase),SOD(superoxide dismutase)and SP(soluble protein),all increased with the increase of HMs stress concentration.

干旱生境中接种丛枝菌根真菌对三叶鬼针草(Bidens pilosa L.)光合特征的影响

为阐明丛枝菌根真菌对石灰岩地区适生植物三叶鬼针草(Bidens pilosaL.)光合作用的影响,设置正常浇水(A)、中度干旱胁迫(B)和重度干旱胁迫(C)3个水分处理梯度,比较了不同水分处理条件下接种丛枝菌根真菌Glomus mosseae和未接种三叶鬼针草之间净光合速率、气孔导度、蒸腾速率、胞间CO2浓度、羧化效率、水分利用效率等特征的差异。结果表明,水分胁迫显著降低三叶鬼针草的净光合速率、气孔导度、蒸腾速率和羧化效率。胞间CO2浓度在处理的前期(7d)因干旱胁迫而降低,在后期随土壤含水量的降低而升高;水分利用效率则是中度胁迫的植株、正常浇水处理植株、重度胁迫植株依次降低。在正常浇水条件下接种G.mosseae对三叶鬼针草光合参数没有产生显著性影响;在中度胁迫条件下,接种植株较未接种植株在水分处理的前28d有更高的净光合速率、气孔导度、蒸腾速率和羧化效率;在重度胁迫条件下,虽然净光合速率、气孔导度、蒸腾速率和羧化效率接种植株高于未接种植株,但是二者并不显著。研究认为,干旱胁迫对三叶鬼针草光合作用的影响在水分处理的前期表现为气孔因素制约,在后期则主要是非气孔因素的影响;在正常浇水条件下接种G.mosseae对三叶鬼针草的光合作用没有显著性影响,在干旱胁迫条件下,丛枝菌根真菌通过改善三叶鬼针草气孔导度和羧化效率等减弱干旱胁迫对植株的伤害,但是这种保护作用因为土壤水分的严重匮乏以及土壤干旱的时间延长而受到限制。

镉、铅胁迫对三叶鬼针草(Bidens pilosa L.)种子萌发的影响

采用沙培法研究铅、镉胁迫对三叶鬼针草种子萌发的影响。结果发现:在胁迫初期,种子的发芽率表现出与对照相同的趋势,后期抑制作用较为明显。低浓度镉、铅胁迫对三叶鬼针草种子的萌发有促进作用;高浓度胁迫对种子的萌发均有较强的抑制作用。

江苏泰县五通组观山段胞石Grahnichitina pilosa的发现及其意义

根据江苏泰县N 4井五通组观山段顶部的胞石Grahnichitinapilosa的发现 ,确定观山段的时代为中泥盆世吉维特期。据此认为 :擂鼓台段的两层暗色泥岩 ,应分别对比为南洞页岩、长顺页岩 ;中泥盆世大海侵时 ,华南海沿苏皖河经皖南、苏南入侵苏北。

不同生境中鬼针草(Bidens pilosa)碳氮磷化学计量特征及其营养利用策略

该研究通过野外采样和实验室测定的方法,研究了三种生境中鬼针草叶和根碳(C)、氮(N)、磷(P)化学计量特征及其与土壤养分的相关性。结果表明:(1)鬼针草的生境具有不同的资源水平。三种生境的土壤全氮(TN)和速效氮贫乏,有机碳(C)和全磷(TP)较充裕;生境Ⅰ土壤TN含量显著低于其它两个生境,生境II土壤TP含量显著低于其它两个生境,生境III土壤TN、TP含量均大于其它两个生境。(2)不同生境的鬼针草对磷(P)分配策略不同。低N生境的鬼针草叶片P含量>根P含量,P较多地分配到植物体地上部分;N、P含量较高的生境中鬼针草根P含量>叶P含量,P更多地分配到地下部分。(3)不同生境的鬼针草其地上部分和根的生长速率不同。低N生境的鬼针草叶片N/P和C/P值小,植物体具有较高的相对生长速率,具有地上生长竞争优势;低P生境的植物叶片N/P和C/P值大,植物体具有较慢的相对生长速率;高N、高P生境中根N/P和C/P值小,根具有较高的生长速率,保证了鬼针草的地下生长竞争优势。(4)鬼针草叶片N/P和根N/P之间呈现不显著的负相关关系,植物地上部分和地下部分为异速生长。不同生境的鬼针草具有不同的营养利用和分配策略,保证了植物强大的竞争力和入侵性。

Analgesic Appraisal of Bidens pilosa (Asteraceae) Leaf Extracts Used in Management of Oral Lesion Pain in HIV/AIDS Patients in Rodents

Oral lesions, diarrhoea, Pneumocystis carinii pneumonia, tuberculosis and urinary tract infections are some of the opportunistic infections (OIs) which arise when the CD4 cells of the HIV/AIDS patient fall below 200 cells/mm3. HIV/AIDS infection complications include tissue damage from oral lesions accompanied with pains. Pain is a disagreeable sensory and sensitive experience associated with actual or potential tissue damage. This condition requires immediate treatment with analgesics and antibiotics. However, the inability of rural dwellers to afford readily available drugs is a consequence for using herbs like Bidens pilosa whose local usefulness in the management of oral lesions of HIV/AIDS has not been proven scientifically. Therefore, the objective of this study was to provide the scientific basis in rats for the traditional healers’ use of Bidens pilosa leaves’ extracts in managing pain associated with oral lesions of HIV/AIDS patients in South Western Uganda. Assessment of the analgesic effects of Bidens pilosa was conducted using acetic acid in mice, formalin-induced pain and tail flick methods in rats. Both aqueous and ethanolic extracts of the leaves of Bidens pilosa produced statistically significant dose dependent inhibition of acetic acid induced pain, non dose dependent pain reduction in formalin induced pain, (p < 0.05;student t-test) and non dose dependent tail withdrawal pattern (p < 0.05, Multivariate ANOVA test). Hence, we conclude that extracts of Bidens pilosa have an analgesic basis for their local use in treatment of oral lesions associated pain in HIV/AIDS patients in South-Western Uganda.

Comparison on the phytoextraction efficiency of Bidens pilosa at heavy metal contaminated site in natural and electrokinetic conditions

The plant samples of Bidens pilosa were collected from a coal gangue vacant site and its surrounding area,located in central China,to study the remediation effect of the plant species on heavy metal(HM)contamination in both natural and electrokinetic(EK)conditions.The analytical results showed that the effect of phytoextraction and bioconcentration on the heavy metals in the sample of the EK group is more significant than those in the other control group.Compared with the results of natural condition,under the EK condition the concentrations of cadmium(Cd),lead(Pb),copper(Cu)and zinc(Zn)in the stems and leaves of the Bidens pilosa increased to 0.40 mg/kg,4.23 mg/kg,7.27 mg/kg,830.24 mg/kg,respectively,with their increments of 292%,1731%,141%,2076%.For root samples,the Cd,Pb,Cu and Zn concentrations increased to 0.52 mg/kg,4.36 mg/kg,10.87 mg/kg,and 98.12 mg/kg and the increase rates were 1034%,140%,29%,and 181%,respectively.The phytoextraction efficiency of the Bidens pilosa was significantly higher than that of control group.The removal efficiency of Cd,Pb,Cu and Zn in soil increased to 26%,72%,27%,and 79%with the EK applied.In addition,the mechanism of HM migration,extraction and enrichment in Bidens pilosa under the EK condition was discussed.

Assessment of in Situ Anthelminthic Activity of Ethanolic Extract of Bidens pilosa against Adult’s Gastro-Intestinal Strongyle of Small Ruminants

Infections caused by gastrointestinal nematodes are major threats to livestock industry in the developing countries. They cause direct effects in form of loss in production and indirect economic losses due to high cost of anthelmintic drugs. Various alternative strategies are in practice to control parasitism which includes the usage of medicinal plants. This study evaluates the in situ anthelminthic activity of Bidens pilosa ethanolic extract on gastro-intestinal strongyles of small ruminants. Twenty (20) naturally infested goats of both sexes were distributed into 5 groups (n = 4) corresponding to groups receiving Tween 80 at 2.8%, Albendazole at 5 mg/kg and the three doses of extract (125, 250 and 500 mg/kg). The doses of extract and 2.8% Tween 80 were administered twice daily for 3 consecutive days while Albendazole was administered in a single dose. Faecal eggs count reduction, body weight, packed cell volume (PCV) and total worm count reduction were determined. Results showed that the ethanolic extract of B. pilosa was not effective against adult worms while Albendozole inflicted a 100% reduction of eggs count and total worm count of digestive strongyle. Body weight and PCV remain almost constant. This study indicates that ethanolic extract of B. pilosa at tested doses has no anthelminthic activity. However, further optimization and standardization of the ethanolic extract of B. pilosa could enhance its anthelminthic activity.

楚雄地区黄龙尾(Agrimonia pilosa var.nepalensis)内生菌的分离及初步鉴定

从野生黄龙尾健康植株内分离得到的29株内生菌中,8株细菌全部为杆菌,有4株产芽孢;10株放线菌,都属于链霉菌属(Streptomyces sp.);11株真菌,其中7株为镰刀菌属(Fusarium sp.)、2株为炭疽菌属(Colletotrichum sp.)、1株为青霉属(Penicillium sp.)、1株为黑曲霉(Aspergillus niger)。生长特性实验结果表明,所有供试菌株的最适生长NaCl浓度范围在3%-5%之间,最适生长pH范围在6.0~8.0之间,最适生长温度在20℃~35℃之间,细菌的生长温度范围比放线菌的更为广泛。在生理生化特性实验中,供试菌株B-3-5-4对碳源、氮源的利用范围广,菌株B-3-1-6对碳源、氮源的利用范围窄。研究结果表明,黄龙尾不同组织内部分布着不同类群的内生菌群。本研究可为黄龙尾的进一步开发提供数据支持。

Comparison of Agrimophol Content in Agrimonia pilosa Ledeb. from Luzhou at Different Harvest Time

[ Objectives] This study was conducted to compare agrimophol contentsin Agrimonia pilosa Ledeb. at different harvest time, so as to select its best harvest time. [Methods] High performance liquid chromatography was used for the determination of agfmophol in A. pilosa Ledeb. at different harvest time in Luzhou. [ Results ] The content of agrimophol could be detected by HPLC. Agrimophol had good linearity in 0.01 - 0.1 mg/ml （ r = 0.999 9 ）, the average recov- ery was 101.5%, and RSD was 1.10%. The content of agrimophol in A. pilosa Ledeb. in Luzhou was 0.010 8% in June, and 0.013 1% in July, much higher than any other months. [ Conclusions] This experiment established a method for detecting the content of agrimophol by HPLC, which is simple, with high precision, high accuracy, good repeatability and recovery. The reasonable harvest time of A. pilosa Ledeb. in Luzhou is June and July.

Study on Seed Dormancy and Germination Characteristics of Portulaca pilosa

To improve understanding of Portulaca pilosa seed germination and lay technical foundation for seedling cultivation,this paper studies the characteristics of dormancy and germination of Portulaca pilosa seed. Research indicates that its primary seed dormancy period is about25 d,dormancy can be completely released by storage at room temperature for 30 d; the seed is positively photoblastic seed that can't sprout and can be induced into dormancy in low light conditions; the dormancy-released seed is more sensitive to light when compared with the freshly picked seed,and the germination peak arises 1 d earlier; Portulaca pilosa seed favorites humid conditions,and the higher the soil moisture,the higher the germination rate and germination energy of the seed. The dormancy-released seeds should be used for Portulaca pilosa seedling cultivation,and after the germination is accelerated for 2- 3 d in the light conditions,the seeds are sown on the soil surface,and covered with transparent plastic film to keep moisture and light,which is conducive to the emergence of seeds.

Hypoglycemic and High Dosage Effects of <i>Bidens pilosa</i>in Type-1 Diabetes Mellitus

Ethno-pharmaceutical products have received a lot of international attention in the scientific community in the management of diabetes mellitus (DM). In this study we determined the anti-diabetic and high dosage effects of Bidens pliosa in type 1 DM (T1DM). Methodology: Thirty rats were divided into six groups and subgrouped into the extract and non extract treatment groups. The extract treated group was subdivided into three groups which received 200 mg/kg, 400 mg/ kg and 800 mg/kg dosage treatments respectively. The blood glucose levels were monitored using a standard glucometer for one month, and biochemical analysis of the two liver function enzymes;Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) were carried out at the Institute of Biomedical Research (IBR-KIU-WC) at the end of week IV. The study revealed that Bidens pilosa maintained hypoglycemia for a period of two weeks and this status was lost in subsequent weeks. T1DM rats treated with a dosage of 200 mg/kg showed a better recovery (355.25 - 164.5 mg/dl) of the glucose levels, followed by those that were being treated at 400 mg/kg. The AST and ALT enzymes in blood varied with a mean ± SEM (33.72 ± 32.32 to -7.23 ± 12.61 IU and 22.98 ± 11.12 to 42 ± 38.2 IU, respectively) in both the glibencimide? and in the 800 mg/ kg treatment groups in the study. High dosages of extract were associated (P = 0.049) with increased systemic enzyme leakage. In conclusion, tissue degeneration caused by high levels of the extract was accompanied by leakage of various enzymes (AST and ALT) into the blood, which could be a major etiological factor for the development of secondary systemic pathologies, thus potentially worsening the effects of an existing T1DM prognosis in human patients. The preliminary results indicate that a dose of Bidens pilosa has an anti-diabetic effect for a limited initial duration before starting to cause systemic toxicological effects. It is highly recommended that further investigation into the cellular mechanisms and consequences of any therapy involving Bidens pilosa be carried out.

In vitro Antibacterial Efficacy of <i>Bidens pilosa</i>, <i>Ageratum conyzoides</i>and <i>Ocimum suave</i>Extracts against HIV/AIDS Patients’ Oral Bacteria in South-Western Uganda

The objective of the study was to determine the antibacterial efficacy of Bidens pilosa Aqueous (BPA), Bidens pilosa Ethanolic (BPE), Ageratum conyzoides Aqueous (ACA), Ageratum conyzoides Ethanolic (ACE), Ocimum suave Aqueous (OSA) and Ocimum suave Ethanolic (OSE) extracts on HIV/AIDS patients’ oral bacteria. Healthy green leaves of the plants were collected in Ishaka Uganda, processed and portions separately extracted with hot distilled water and cold ethanol. The susceptibility, MIC and MBC of each extract were determined using standard protocols. The bacteria had significant (p Bidens pilosa, Ageratum conyzoides and Ocimum suave on oral lesions of HIV/AIDS patients made by traditional healers and local people in South-Western Uganda. We recommend a detailed study of structural identities and activities of the active antibacterial principle(s) in these plants for possible new drug entities and verification of the interactive effects of the principle(s) with ARVs and cotrimoxazole used daily by HIV/AIDS patients.

In Vitro Evaluation of Sida pilosa Retz (Malvaceae) Aqueous Extract and Derived Fractions on Schistosoma mansoni

Sida pilosa Retz. (Malvaceae) is a medicinal plant used in Africa for the treatment of dysmenorrhea, lower abdominal pains and intestinal helminthiasis. S. pilosa aqueous extract and derived fractions were investigated for their bioactivity against Schistosoma mansoni. The aqueous extract from S. pilosa aerial parts (1.25 - 40 mg/mL) and derived fractions (n-hexane, DCM, EtOAc and n-BuOH: 0.25 - 8 mg/mL) were tested on adult S. mansoni maintained in a GMEN culture medium. Praziquantel was used as the reference drug. After 24 h of incubation, worms were monitored for their viability and egg output. The antioxidant activity of S. pilosa was evaluated by the ability to scavenge the 2,2-diphenyl-1-picrylhydrazyl free radicals. The chemical composition of the n-BuOH fraction was investigated by HPLC-MS analysis. S. pilosa aqueous extract and fractions significantly increased worm mortality in a concentration-dependent manner. The n-BuOH fraction was the most active with a LC50 of 1.25 mg/mL. Significant reduction of motor activity (25% to 100%) was recorded for surviving worms incubated in different concentrations of the extract and fractions. Incubation of S. mansoni in different concentrations of S. pilosa extract and fractions led to significant reduction of egg laying (52% to 100%). The aqueous extract and derived fractions exhibited antioxidant activity in a concentration-dependent manner. The highest antioxidant activity was found with the EtOAc fraction, followed by the DCM and n-BuOH fractions. HPLC-MS analysis of the n-butanol fraction revealed the presence of two indoloquinoline alkaloids. This study disclosed the schistosomicidal activity of the n-butanol fraction from S. pilosa aqueous extract. This activity is probably related to the indoloquinoline alkaloids identified in the fraction.

Analysis and Assessment of Agrimonia Pilosa Ledeb from Different Sources Using FT-IR Spectroscopy

To get the IR spectrums of Agrimonia Pilosa Ledeb(APL)from China and Japan areas,and to find out the characters of IR spectrums through the content of different chemical constituents,to provide a fast and effective analysis method monitor the inherent qualities of traditional Chinese medicine-APL.Fourier Transform Infrared Spectroscopy(FT-IR)was applied to detect sample of APL from China and Japan areas.This study showed that the IR spectra of APL from China and Japan areas have their unique IR fingerprint features.The contents of tannin and calcium phosphate in APL from China is different APL from Japan.So FT-IR is a very quick,effective and well repetitive method for monitoring and distinguishing the traditional Chinese medicine.

鬼针草对重金属镉的耐受性和抗氧化酶响应特征研究

采用营养液栽培的方式,设置不同浓度Cd(0、20、40、80 mg/L和160 mg/L)胁迫处理,探究不同浓度下鬼针草的生长、耐受能力和抗氧化酶变化特征.结果表明:随Cd胁迫浓度升高,鬼针草生长受抑制加重,株高和生物量显著下降,Cd浓度为20 mg/L时根系耐性系数达最大为0.60,高于该浓度鬼针草生长发育受阻.植株Cd含量随胁迫浓度增加而升高,根部最高含量达1203.64 mg/kg,Cd1(20 mg/L)处理时Cd富集总量最大,后逐渐减小.抗氧化酶活性(过氧化物酶、超氧化物歧化酶和过氧化氢酶)呈现先升后降再升高的趋势,表现出对Cd胁迫的响应过程,其中超氧化物歧化酶活性变化与鬼针草Cd的吸收呈极显著正相关(P<0.01).研究表明鬼针草对Cd有较强吸收能力,适用于有效Cd浓度低于20 mg/L的污染土壤修复.

仙鹤草HPLC指纹图谱建立及化学计量学分析

目的建立仙鹤草的HPLC指纹图谱,并结合化学计量学分析评价不同产地的仙鹤草药材整体质量。方法采用YMC-Pack ODS-A色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,流速1.0 ml/min,柱温30℃,检测波长270 nm;进样量10μl。建立HPLC指纹图谱并进行相似度评价,结合聚类分析(CA)、主成分分析(PCA)、偏最小二乘判别分析(PLS-DA)对15批样品进行分析。结果HPLC指纹图谱共标定14个共有峰,指认4个成分,分别为1号峰没食子酸,4号峰儿茶素,8号峰鞣花酸,10号峰槲皮苷;15批仙鹤草指纹图谱相似度0.778~0.978,表明不同产地仙鹤草质量存在差异,经CA、PCA分析样品分为两类,结合PLS-DA筛选出6个差异标志物。结论不同来源的仙鹤草药材存在一定的质量差异。通过指纹图谱、聚类分析、主成分分析及偏最小二乘法判别分析等方法相结合可全面地评价仙鹤草的质量,此方法的建立可以为仙鹤草的质量控制、产品评价提供一定参考依据。